GenomeNet

Database: Pfam
Entry: GPS
LinkDB: GPS
Original site: GPS 
#=GF ID   GPS
#=GF AC   PF01825.24
#=GF DE   GPCR proteolysis site, GPS, motif 
#=GF PI   GPS; GAIN; GAIN-GPS;
#=GF AU   Bateman A;0000-0002-6982-4660
#=GF SE   SMART
#=GF GA   22.50 22.50;
#=GF TC   22.50 22.50;
#=GF NC   22.40 22.40;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Motif
#=GF CL   CL0661
#=GF RN   [1]
#=GF RM   9920906
#=GF RT   Structural requirements for alpha-latrotoxin binding and alpha-
#=GF RT   latrotoxin-stimulated secretion. A study with
#=GF RT   calcium-independent receptor of alpha-latrotoxin (CIRL) deletion
#=GF RT   mutants. 
#=GF RA   Krasnoperov V, Bittner MA, Holz RW, Chepurny O, Petrenko AG; 
#=GF RL   J Biol Chem 1999;274:3590-3596.
#=GF RN   [2]
#=GF RM   9830014
#=GF RT   alpha-Latrotoxin receptor CIRL/latrophilin 1 (CL1) defines an
#=GF RT   unusual family of ubiquitous G-protein-linked receptors.
#=GF RT   G-protein coupling not required for triggering exocytosis. 
#=GF RA   Sugita S, Ichtchenko K, Khvotchev M, Sudhof TC; 
#=GF RL   J Biol Chem 1998;273:32715-32724.
#=GF RN   [3]
#=GF RM   10469603
#=GF RT   A latrophilin/CL-1-like GPS domain in polycystin-1. 
#=GF RA   Ponting CP, Hofmann K, Bork P; 
#=GF RL   Curr Biol 1999;26:585-588.
#=GF RN   [4]
#=GF RM   17525154
#=GF RT   Characterization of cis-autoproteolysis of polycystin-1, the
#=GF RT   product of human polycystic kidney disease 1 gene. 
#=GF RA   Wei W, Hackmann K, Xu H, Germino G, Qian F; 
#=GF RL   J Biol Chem. 2007;282:21729-21737.
#=GF RN   [5]
#=GF RM   12270923
#=GF RT   Post-translational proteolytic processing of the
#=GF RT   calcium-independent receptor of  alpha-latrotoxin (CIRL), a
#=GF RT   natural chimera of the cell adhesion protein and the G
#=GF RT   protein-coupled receptor. Role of the G protein-coupled receptor
#=GF RT   proteolysis site (GPS) motif.
#=GF RA   Krasnoperov V, Lu Y, Buryanovsky L, Neubert TA, Ichtchenko K,
#=GF RA   Petrenko AG;
#=GF RL   J Biol Chem. 2002;277:46518-46526.
#=GF RN   [6]
#=GF RM   22333914
#=GF RT   A novel evolutionarily conserved domain of cell-adhesion GPCRs
#=GF RT   mediates autoproteolysis.
#=GF RA   Arac D, Boucard AA, Bolliger MF, Nguyen J, Soltis SM, Sudhof TC,
#=GF RA   Brunger AT;
#=GF RL   EMBO J. 2012;31:1364-1378.
#=GF DR   INTERPRO; IPR000203;
#=GF DR   SMART; GPS;
#=GF DR   MEROPS; T6;
#=GF DR   SO; 0001067; polypeptide_motif;
#=GF CC   The GPS motif is found in GPCRs, and is the site for
#=GF CC   auto-proteolysis, so is thus named, GPS [1,2,3,4]. The GPS motif
#=GF CC   is a conserved sequence of ~40 amino acids containing canonical
#=GF CC   cysteine and tryptophan residues, and is the most highly
#=GF CC   conserved part of the domain. In most, if not all, cell-adhesion
#=GF CC   GPCRs these undergo autoproteolysis in the GPS between a
#=GF CC   conserved aliphatic residue (usually a leucine) and a threonine,
#=GF CC   serine, or cysteine residue [5]. In higher eukaryotes this motif
#=GF CC   is found embedded in the C-terminal beta-stranded part of a GAIN
#=GF CC   domain - GPCR-Autoproteolysis INducing (GAIN). The GAIN-GPS
#=GF CC   domain adopts a fold in which the GPS motif, at the C-terminus,
#=GF CC   forms five beta-strands that are tightly integrated into the
#=GF CC   overall GAIN domain. The GPS motif, evolutionarily conserved
#=GF CC   from tetrahymena to mammals, is the only extracellular domain
#=GF CC   shared by all human cell-adhesion GPCRs and PKD proteins, and is
#=GF CC   the locus of multiple human disease mutations. The GAIN-GPS
#=GF CC   domain is both necessary and sufficient functionally for
#=GF CC   autoproteolysis, suggesting an autoproteolytic mechanism whereby
#=GF CC   the overall GAIN domain fine-tunes the chemical environment in
#=GF CC   the GPS to catalyse peptide bond hydrolysis [6]. In the
#=GF CC   cell-adhesion GPCRs and PKD proteins, the GPS motif is always
#=GF CC   located at the end of their long N-terminal extracellular
#=GF CC   regions, immediately before the first transmembrane helix of the
#=GF CC   respective protein.
#=GF SQ   17360
#=GS A0A671YRK3_SPAAU/774-818    AC A0A671YRK3.1
#=GS A0A6P4Y4H0_BRABE/110-158    AC A0A6P4Y4H0.1
#=GS A0A6P8USS4_GYMAC/439-481    AC A0A6P8USS4.1
#=GS A0A6P4YTL9_BRABE/2567-2607  AC A0A6P4YTL9.1
#=GS A0A2I3TMZ4_PANTR/548-590    AC A0A2I3TMZ4.1
#=GS A0A6P8WSQ4_GYMAC/773-817    AC A0A6P8WSQ4.1
#=GS A0A093EJU2_TYTAL/758-802    AC A0A093EJU2.1
#=GS A0A1S3IC84_LINUN/3050-3091  AC A0A1S3IC84.1
#=GS K7E7I1_ORNAN/86-124         AC K7E7I1.2
#=GS A0A3P9MT75_POERE/791-835    AC A0A3P9MT75.1
#=GS A0A384B2L8_BALAS/989-1033   AC A0A384B2L8.1
#=GS A0A5F8GJ22_MONDO/73-108     AC A0A5F8GJ22.1
#=GS H0ZSL3_TAEGU/959-1002       AC H0ZSL3.2
#=GS A0A1Y3BPD0_EURMA/217-262    AC A0A1Y3BPD0.1
#=GS A0A1S3IQY4_LINUN/1345-1389  AC A0A1S3IQY4.1
#=GS A0A3B5PWQ3_XIPMA/724-769    AC A0A3B5PWQ3.1
#=GS A0A673YJM6_SALTR/421-466    AC A0A673YJM6.1
#=GS A0A3Q1HK07_ANATE/1077-1118  AC A0A3Q1HK07.2
#=GS A0A3Q7SRG9_VULVU/884-928    AC A0A3Q7SRG9.1
#=GS A0A091T2A5_PELCR/9-53       AC A0A091T2A5.1
#=GS A0A6J3B3V5_VICPA/786-830    AC A0A6J3B3V5.1
#=GS A0A2K5VUE8_MACFA/2453-2498  AC A0A2K5VUE8.2
#=GS A0A6P6JK57_CARAU/807-851    AC A0A6P6JK57.1
#=GS L5LH99_MYODS/16-52          AC L5LH99.1
#=GS A0A2K5KX80_CERAT/800-845    AC A0A2K5KX80.1
#=GS A0A287B0F5_PIG/442-485      AC A0A287B0F5.1
#=GS A0A2Y9IJ99_ENHLU/2615-2659  AC A0A2Y9IJ99.1
#=GS A0A267DL10_9PLAT/509-548    AC A0A267DL10.1
#=GS A0A1A6HPL1_NEOLE/15-50      AC A0A1A6HPL1.1
#=GS F1R5S5_DANRE/90-134         AC F1R5S5.1
#=GS A0A6P8IAN5_ACTTE/653-699    AC A0A6P8IAN5.1
#=GS A0A2K6EMM4_PROCO/755-800    AC A0A2K6EMM4.1
#=GS A0A6J0TG78_9SAUR/809-853    AC A0A6J0TG78.1
#=GS A0A1S3LBX9_SALSA/2429-2473  AC A0A1S3LBX9.1
#=GS A0A668V7X7_OREAU/841-885    AC A0A668V7X7.1
#=GS F6US58_CIOIN/2164-2211      AC F6US58.2
#=GS A0A2K5F1T0_AOTNA/773-817    AC A0A2K5F1T0.1
#=GS A0A383Z852_BALAS/841-886    AC A0A383Z852.1
#=GS G3TEN7_LOXAF/793-837        AC G3TEN7.1
#=GS A0A7M7N283_STRPU/487-528    AC A0A7M7N283.1
#=GS H9GED6_ANOCA/545-589        AC H9GED6.1
#=GS AGRL2_RAT/790-834           AC O88923.2
#=GS A0A091DKW7_FUKDA/27-69      AC A0A091DKW7.1
#=GS A0A6A5FJI2_PERFL/282-321    AC A0A6A5FJI2.1
#=GS A0A401RQB7_CHIPU/289-331    AC A0A401RQB7.1
#=GS A0A3Q0CVI3_MESAU/556-596    AC A0A3Q0CVI3.1
#=GS A0A7R5KC47_9PASS/824-868    AC A0A7R5KC47.1
#=GS A0A3Q7WTG9_URSAR/382-427    AC A0A3Q7WTG9.1
#=GS A0A1S3LD88_SALSA/2429-2473  AC A0A1S3LD88.1
#=GS A0A6Q2Y782_ESOLU/813-858    AC A0A6Q2Y782.1
#=GS A0A673IM26_9TELE/733-777    AC A0A673IM26.1
#=GS T1IIR4_STRMM/628-675        AC T1IIR4.1
#=GS A0A341BKB5_NEOAA/180-225    AC A0A341BKB5.1
#=GS A0A226PIU5_COLVI/945-986    AC A0A226PIU5.1
#=GS A0A673UCW6_SURSU/859-904    AC A0A673UCW6.1
#=GS A0A091F2G0_CORBR/95-137     AC A0A091F2G0.1
#=GS G3RK52_GORGO/884-928        AC G3RK52.2
#=GS A0A287BC71_PIG/381-421      AC A0A287BC71.1
#=GS A0A669EU23_ORENI/804-849    AC A0A669EU23.1
#=GS A0A3P8VCC3_CYNSE/795-839    AC A0A3P8VCC3.1
#=GS A0A665WYU4_ECHNA/2429-2474  AC A0A665WYU4.1
#=GS A0A5F4CKN8_CANLF/247-289    AC A0A5F4CKN8.1
#=GS A0A7E6F2A7_OCTVU/835-879    AC A0A7E6F2A7.1
#=GS A0A074ZM52_9TREM/1976-2021  AC A0A074ZM52.1
#=GS A0A6Q2XAH1_ESOLU/968-1012   AC A0A6Q2XAH1.1
#=GS A0A455APD4_PHYMC/850-899    AC A0A455APD4.1
#=GS A0A6P6JSH6_CARAU/761-805    AC A0A6P6JSH6.1
#=GS A0A7R5KC40_9PASS/824-868    AC A0A7R5KC40.1
#=GS A0A6J2R3E5_COTGO/815-860    AC A0A6J2R3E5.1
#=GS A0A6P5LX81_PHACI/571-615    AC A0A6P5LX81.1
#=GS A0A672GUS7_SALFA/813-857    AC A0A672GUS7.1
#=GS A0A3Q1FR14_9TELE/1321-1362  AC A0A3Q1FR14.1
#=GS A0A667XU58_9TELE/262-306    AC A0A667XU58.1
#=GS A0A6P5AAU9_BRABE/2116-2156  AC A0A6P5AAU9.1
#=GS A0A091JW42_COLST/387-431    AC A0A091JW42.1
#=GS M7BJM6_CHEMY/475-518        AC M7BJM6.1
#=GS A0A2K6QDQ6_RHIRO/644-689    AC A0A2K6QDQ6.1
#=GS A0A6J1ZCU1_ACIJB/777-821    AC A0A6J1ZCU1.1
#=GS H2YF53_CIOSA/1-40           AC H2YF53.1
#=GS A0A6P7IV17_9TELE/2902-2947  AC A0A6P7IV17.1
#=GS A0A341CHZ2_NEOAA/537-579    AC A0A341CHZ2.1
#=GS A0A668UGW4_OREAU/371-413    AC A0A668UGW4.1
#=GS A0A3Q0EAN7_CARSF/805-850    AC A0A3Q0EAN7.1
#=GS A0A091HEU3_BUCRH/374-412    AC A0A091HEU3.1
#=GS A0A6P5CCM0_BOSIN/773-818    AC A0A6P5CCM0.1
#=GS A0A671TRM1_SPAAU/808-853    AC A0A671TRM1.1
#=GS A0A232FMS6_9HYME/660-705    AC A0A232FMS6.1
#=GS A0A498LDW1_LABRO/120-160    AC A0A498LDW1.1
#=GS G3VRU6_SARHA/345-389        AC G3VRU6.2
#=GS F7AR30_ORNAN/2261-2305      AC F7AR30.3
#=GS H9GWK0_CANLF/170-208        AC H9GWK0.2
#=GS A0A671Y6T3_SPAAU/5749-5797  AC A0A671Y6T3.1
#=GS A0A286XGC3_CAVPO/786-830    AC A0A286XGC3.1
#=GS A0A3P9PG49_POERE/811-855    AC A0A3P9PG49.1
#=GS A0A553RFF3_9TELE/488-529    AC A0A553RFF3.1
#=GS A0A6J0CH93_PERMB/799-843    AC A0A6J0CH93.1
#=GS A0A3Q3ABB9_CHICK/512-556    AC A0A3Q3ABB9.1
#=GS A0A5F5XMN3_FELCA/618-661    AC A0A5F5XMN3.1
#=GS A0A6A5DX85_PERFL/1072-1112  AC A0A6A5DX85.1
#=GS A0A2K6DDV9_MACNE/786-830    AC A0A2K6DDV9.1
#=GS A0A2K6Q7J0_RHIRO/444-487    AC A0A2K6Q7J0.1
#=GS A0A673H2R5_9TELE/777-824    AC A0A673H2R5.1
#=GS A0A4W4DY24_ELEEL/42-88      AC A0A4W4DY24.1
#=GS A0A6P8VPF8_GYMAC/859-904    AC A0A6P8VPF8.1
#=GS A0A1S3A0F6_ERIEU/889-933    AC A0A1S3A0F6.1
#=GS A0A2R9B8I2_PANPA/392-435    AC A0A2R9B8I2.1
#=GS A0A060Y7I5_ONCMY/602-647    AC A0A060Y7I5.1
#=GS A0A6P8EQW4_CLUHA/476-513    AC A0A6P8EQW4.1
#=GS A0A485NY96_LYNPA/994-1038   AC A0A485NY96.1
#=GS A0A7N8XEW1_9TELE/781-825    AC A0A7N8XEW1.1
#=GS A0A383ZIR7_BALAS/772-816    AC A0A383ZIR7.1
#=GS H0W4I1_CAVPO/241-283        AC H0W4I1.2
#=GS A0A452IQ45_9SAUR/32-68      AC A0A452IQ45.1
#=GS A0A5K1UFM2_CALJA/2317-2362  AC A0A5K1UFM2.1
#=GS A0A2K5PXL2_CEBIM/547-591    AC A0A2K5PXL2.1
#=GS A0A6P8IHF1_ACTTE/539-583    AC A0A6P8IHF1.1
#=GS A0A3B3IGY3_ORYLA/3097-3142  AC A0A3B3IGY3.1
#=GS A0A6J3QG02_TURTR/77-122     AC A0A6J3QG02.1
#=GS A0A5G2QWR4_PIG/447-490      AC A0A5G2QWR4.1
#=GS K7FHJ6_PELSI/514-549        AC K7FHJ6.1
#=GS A0A2K6MQN9_RHIBE/489-532    AC A0A2K6MQN9.1
#=GS A0A671VFN4_SPAAU/861-906    AC A0A671VFN4.1
#=GS A0A2K6QE19_RHIRO/2478-2523  AC A0A2K6QE19.1
#=GS A0A3B3HBE5_ORYLA/859-904    AC A0A3B3HBE5.1
#=GS A0A672NC49_SINGR/857-901    AC A0A672NC49.1
#=GS A0A671EV73_RHIFE/544-588    AC A0A671EV73.1
#=GS A0A093DLN0_CHAPE/329-372    AC A0A093DLN0.1
#=GS A0A3Q7NRY8_CALUR/641-686    AC A0A3Q7NRY8.1
#=GS A0A6P6KIX4_CARAU/803-847    AC A0A6P6KIX4.1
#=GS A0A553PWE2_9TELE/5097-5138  AC A0A553PWE2.1
#=GS A0A6J1ZC00_ACIJB/790-834    AC A0A6J1ZC00.1
#=GS A0A3Q2H2P2_HORSE/790-834    AC A0A3Q2H2P2.1
#=GS A0A3Q2CJ85_CYPVA/791-835    AC A0A3Q2CJ85.1
#=GS A0A6P7LX76_BETSP/791-835    AC A0A6P7LX76.1
#=GS A0A099ZNM2_TINGU/834-877    AC A0A099ZNM2.1
#=GS A0A4W5KPY5_9TELE/374-418    AC A0A4W5KPY5.1
#=GS A0A674MML2_TAKRU/787-831    AC A0A674MML2.1
#=GS A0A2K6V7G0_SAIBB/569-613    AC A0A2K6V7G0.1
#=GS A0A665WYG2_ECHNA/2142-2187  AC A0A665WYG2.1
#=GS A0A673ZNH3_SALTR/482-524    AC A0A673ZNH3.1
#=GS A0A484CPW1_PERFV/786-830    AC A0A484CPW1.1
#=GS A0A485MGS4_LYNPA/2325-2370  AC A0A485MGS4.1
#=GS A0A671Y956_SPAAU/873-917    AC A0A671Y956.1
#=GS A0A3Q2DUM7_CYPVA/206-246    AC A0A3Q2DUM7.1
#=GS A0A672SEV7_SINGR/2367-2412  AC A0A672SEV7.1
#=GS A0A6P6RLP9_CARAU/399-440    AC A0A6P6RLP9.1
#=GS A0A553QYP7_9TELE/650-702    AC A0A553QYP7.1
#=GS A0A673IM53_9TELE/733-777    AC A0A673IM53.1
#=GS H2M385_ORYLA/819-864        AC H2M385.2
#=GS A0A2K5U820_MACFA/798-843    AC A0A2K5U820.2
#=GS A0A096MDE5_POEFO/792-836    AC A0A096MDE5.1
#=GS A0A2K5ZBT9_MANLE/187-232    AC A0A2K5ZBT9.1
#=GS A0A1S3PSM4_SALSA/344-386    AC A0A1S3PSM4.1
#=GS A0A6J2W3T2_CHACN/683-723    AC A0A6J2W3T2.1
#=GS A0A3Q4GX60_NEOBR/291-332    AC A0A3Q4GX60.1
#=GS A0A384C5C4_URSMA/535-575    AC A0A384C5C4.1
#=GS A0A287BDX1_PIG/720-764      AC A0A287BDX1.1
#=GS A0A3P8THV7_AMPPE/80-123     AC A0A3P8THV7.1
#=GS E7ENK1_HUMAN/816-860        AC E7ENK1.1
#=GS A0A6J0E8F1_PERMB/229-274    AC A0A6J0E8F1.1
#=GS T1FD34_HELRO/3-43           AC T1FD34.1
#=GS A0A3Q7SXA5_VULVU/773-817    AC A0A3Q7SXA5.1
#=GS A0A6J1UBR0_9SAUR/1281-1322  AC A0A6J1UBR0.1
#=GS I3KIY9_ORENI/39-84          AC I3KIY9.2
#=GS A0A2K5K0X8_COLAP/805-850    AC A0A2K5K0X8.1
#=GS A0A2U4B8T6_TURTR/300-342    AC A0A2U4B8T6.1
#=GS A0A671EJB6_RHIFE/303-345    AC A0A671EJB6.1
#=GS A0A553N3G9_9TELE/586-630    AC A0A553N3G9.1
#=GS A0A6P4YJ35_BRABE/29-69      AC A0A6P4YJ35.1
#=GS A0A663EUM7_AQUCH/2247-2292  AC A0A663EUM7.1
#=GS A0A0G4EFL7_VITBC/2713-2755  AC A0A0G4EFL7.1
#=GS A0A673GFT6_9TELE/555-593    AC A0A673GFT6.1
#=GS G1PDA0_MYOLU/214-256        AC G1PDA0.1
#=GS A0A3M0K5U3_HIRRU/709-753    AC A0A3M0K5U3.1
#=GS A7RGF9_NEMVE/1923-1963      AC A7RGF9.1
#=GS A0A3M6U9T4_POCDA/366-411    AC A0A3M6U9T4.1
#=GS A0A5N4DQ74_CAMDR/280-325    AC A0A5N4DQ74.1
#=GS A0A663FHG9_AQUCH/203-249    AC A0A663FHG9.1
#=GS A0A671PMF0_9TELE/860-904    AC A0A671PMF0.1
#=GS A0A1L8G3F4_XENLA/1-35       AC A0A1L8G3F4.1
#=GS A0A3Q2H3S5_HORSE/952-996    AC A0A3Q2H3S5.1
#=GS A0A3P9PAX8_POERE/3069-3114  AC A0A3P9PAX8.1
#=GS A0A3Q2TWY2_CHICK/440-484    AC A0A3Q2TWY2.1
#=GS A0A7M7NQA6_STRPU/196-251    AC A0A7M7NQA6.1
#=GS A0A3Q1G4P5_9TELE/1-33       AC A0A3Q1G4P5.1
#=GS A0A1D2N1S9_ORCCI/561-593    AC A0A1D2N1S9.1
#=GS A0A1S3JJW8_LINUN/61-106     AC A0A1S3JJW8.1
#=GS A0A099Z184_TINGU/762-806    AC A0A099Z184.1
#=GS A0A6G0HT77_LARCR/862-907    AC A0A6G0HT77.1
#=GS A0A6J1ZFZ3_ACIJB/804-849    AC A0A6J1ZFZ3.1
#=GS H9H0N4_MELGA/567-612        AC H9H0N4.1
#=GS A0A0L8GEB5_OCTBM/1191-1239  AC A0A0L8GEB5.1
#=GS A0A2P6N9P3_9EUKA/1106-1150  AC A0A2P6N9P3.1
#=GS A0A384D169_URSMA/205-250    AC A0A384D169.1
#=GS A0A667XKB3_9TELE/2590-2635  AC A0A667XKB3.1
#=GS H2MLE4_ORYLA/668-707        AC H2MLE4.2
#=GS A0A341BH59_NEOAA/599-641    AC A0A341BH59.1
#=GS A0A452C9F9_BALAS/813-858    AC A0A452C9F9.1
#=GS A0A668UCL5_OREAU/158-200    AC A0A668UCL5.1
#=GS L9L0T5_TUPCH/754-790        AC L9L0T5.1
#=GS A0A2Y9JP86_ENHLU/874-918    AC A0A2Y9JP86.1
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