GenomeNet

Database: Pfam
Entry: MmeI_C
LinkDB: MmeI_C
Original site: MmeI_C 
#=GF ID   MmeI_C
#=GF AC   PF20467.1
#=GF DE   MmeI, C-terminal domain
#=GF AU   Paysan-Lafosse T;0000-0001-5663-0894
#=GF SE   MGnify-UniProt clustering
#=GF GA   27.00 27.00;
#=GF TC   27.00 27.10;
#=GF NC   26.80 26.90;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Domain
#=GF RC   Paper describing PDB structure 5hr4
#=GF RN   [1]
#=GF RM   27082731
#=GF RT   Structure of Type IIL Restriction-Modification Enzyme MmeI in
#=GF RT   Complex with DNA Has Implications for Engineering New
#=GF RT   Specificities.
#=GF RA   Callahan SJ, Luyten YA, Gupta YK, Wilson GG, Roberts RJ, Morgan
#=GF RA   RD, Aggarwal AK;
#=GF RL   PLoS Biol. 2016;14:e1002442.
#=GF RN   [2]
#=GF RM   18931376
#=GF RT   MmeI: a minimal Type II restriction-modification system that
#=GF RT   only modifies one DNA strand for host protection.
#=GF RA   Morgan RD, Bhatia TK, Lovasco L, Davis TB;
#=GF RL   Nucleic Acids Res. 2008;36:6558-6570.
#=GF RN   [3]
#=GF RM   3016643
#=GF RT   Isolation and computer-aided characterization of MmeI, a type II
#=GF RT   restriction endonuclease from Methylophilus methylotrophus.
#=GF RA   Boyd AC, Charles IG, Keyte JW, Brammar WJ;
#=GF RL   Nucleic Acids Res. 1986;14:5255-5274.
#=GF RN   [4]
#=GF RM   9858752
#=GF RT   Two intertwined methylation activities of the MmeI
#=GF RT   restriction-modification class-IIS system from Methylophilus
#=GF RT   methylotrophus.
#=GF RA   Tucholski J, Zmijewski JW, Podhajska AJ;
#=GF RL   Gene. 1998;223:293-302.
#=GF DR   SO; 0000417; polypeptide_domain;
#=GF CC   Type IIL Restriction-Modification Enzyme MmeI is a large enzyme
#=GF CC   that integrates DNA recognition and methyltransferase and
#=GF CC   endonuclease activities within the same polypeptide [2, 3, 4].
#=GF CC   MmeI is composed of five domains. An N-terminal endonuclease
#=GF CC   domain (residues 1-155), connects to a DNA-methyltransferase
#=GF CC   domain (MTase, residues  301-320) via a multi-helical spacer
#=GF CC   (residues 156-300).  These are followed by the target
#=GF CC   recognition domain (TRD, residues 621-825), and a final
#=GF CC   C-terminal helical bundle (residues 826-919) [1]. The DNA is
#=GF CC   embedded between the TRD and the MTase domain. The TRD makes
#=GF CC   contacts to the DNA bases primarily in the major groove, while
#=GF CC   the MTase domain makes several contacts to the DNA in the minor
#=GF CC   groove [1]. This domain corresponds to the C-terminal region.
#=GF SQ   674
#=GS A0A2V1ITJ4_9BACT/752-826    AC A0A2V1ITJ4.1
#=GS A0A0P7KKD6_9RHOB/807-889    AC A0A0P7KKD6.1
#=GS A0A1I2GE58_9PROT/306-388    AC A0A1I2GE58.1
#=GS A0A087DP47_9BIFI/848-929    AC A0A087DP47.1
#=GS A0A5C6RHM1_9BACT/833-915    AC A0A5C6RHM1.1
#=GS C8NB92_CARH6/832-917        AC C8NB92.1
#=GS E8LIJ7_SUCHY/499-580        AC E8LIJ7.1
#=GS A0A5B7ZND6_9GAMM/833-915    AC A0A5B7ZND6.1
#=GS A0A1W2EBF4_9RHIZ/138-210    AC A0A1W2EBF4.1
#=GS A0A2T5ZYC5_9RHOB/66-148     AC A0A2T5ZYC5.1
#=GS A0A364RHH2_9BACT/34-116     AC A0A364RHH2.1
#=GS A0A3A4FIR7_9MICC/844-923    AC A0A3A4FIR7.1
#=GS A0A4Q2SCA2_9RHIZ/1-80       AC A0A4Q2SCA2.1
#=GS A0A552A5S6_MICAE/313-395    AC A0A552A5S6.1
#=GS A0A1G9X3P9_9SPHI/821-903    AC A0A1G9X3P9.1
#=GS E5Y919_BILW3/824-906        AC E5Y919.1
#=GS A0A418Q7B9_9CORY/860-942    AC A0A418Q7B9.1
#=GS A0A3A4F6L8_9MICC/865-949    AC A0A3A4F6L8.1
#=GS A0A3A9G515_9FIRM/842-923    AC A0A3A9G515.1
#=GS A0A3S0VAG2_9MICC/1-64       AC A0A3S0VAG2.1
#=GS A0A5P8E9G2_9BACT/843-925    AC A0A5P8E9G2.1
#=GS A0A0P6WXK1_9SPHN/835-916    AC A0A0P6WXK1.1
#=GS A0A1J6WZ98_9BACI/840-922    AC A0A1J6WZ98.1
#=GS G6EF39_9SPHN/835-916        AC G6EF39.1
#=GS A0A521DWQ4_9RHOB/832-914    AC A0A521DWQ4.1
#=GS A0A1I5JMG2_9BACT/893-975    AC A0A1I5JMG2.1
#=GS A0A1I1P041_9BACT/821-915    AC A0A1I1P041.1
#=GS A0A2G8R7Y3_9RHOB/824-906    AC A0A2G8R7Y3.1
#=GS A0A4V3AMY5_9GAMM/837-927    AC A0A4V3AMY5.1
#=GS H6LJZ8_ACEWD/845-927        AC H6LJZ8.1
#=GS Q3JF55_NITOC/816-897        AC Q3JF55.1
#=GS A0A2S7XM57_9GAMM/824-906    AC A0A2S7XM57.1
#=GS W1TVE4_9ACTO/1-69           AC W1TVE4.1
#=GS A0A0L6CH57_9MICO/809-891    AC A0A0L6CH57.1
#=GS A0A418VFH6_9DEIO/169-254    AC A0A418VFH6.1
#=GS A0A0Q7I2D0_9RHIZ/842-924    AC A0A0Q7I2D0.1
#=GS A0A4Y7RCT2_9FIRM/830-913    AC A0A4Y7RCT2.1
#=GS A0A3P1SH52_9ACTO/846-927    AC A0A3P1SH52.1
#=GS A0A6N9PZF7_9FIRM/843-925    AC A0A6N9PZF7.1
#=GS I3Z8S6_BELBD/809-891        AC I3Z8S6.1
#=GS D6XYY1_BACIE/805-884        AC D6XYY1.1
#=GS A0A5B8NTZ8_9CORY/491-578    AC A0A5B8NTZ8.1
#=GS A0A2G1QHE7_9RHIZ/833-914    AC A0A2G1QHE7.1
#=GS Q2GBR4_NOVAD/821-902        AC Q2GBR4.1
#=GS A0A1H9TYY5_9CORY/25-112     AC A0A1H9TYY5.1
#=GS A0A196QUB9_9RHOB/824-906    AC A0A196QUB9.1
#=GS A0A1I1GAF0_9LACO/839-917    AC A0A1I1GAF0.1
#=GS A0A1M5EDC8_9FLAO/805-887    AC A0A1M5EDC8.1
#=GS A0A4R5PIK0_9RHIZ/825-906    AC A0A4R5PIK0.1
#=GS A0A1G6D8X3_9FIRM/845-928    AC A0A1G6D8X3.1
#=GS R7F520_9BACT/835-915        AC R7F520.1
#=GS A0A1T4Q9I1_9SPIO/1017-1095  AC A0A1T4Q9I1.1
#=GS A0A5C8Z533_9GAMM/826-907    AC A0A5C8Z533.1
#=GS C0QCT5_DESAH/795-876        AC C0QCT5.1
#=GS X5EG18_9CORY/851-932        AC X5EG18.1
#=GS A0A0B8Y406_9SPHI/866-948    AC A0A0B8Y406.1
#=GS A0A2S6FU25_9CLOT/833-915    AC A0A2S6FU25.1
#=GS A0A1H6S5J7_9GAMM/817-898    AC A0A1H6S5J7.1
#=GS A0A0U5BEW5_9PROT/810-891    AC A0A0U5BEW5.1
#=GS A0A1M7KEC6_9RHOB/832-914    AC A0A1M7KEC6.1
#=GS A0A160F1B0_9BACI/829-911    AC A0A160F1B0.1
#=GS A0A099S3X3_9CLOT/828-908    AC A0A099S3X3.1
#=GS W7YB08_9BACT/185-277        AC W7YB08.1
#=GS A0A1H3YBQ5_9BURK/832-914    AC A0A1H3YBQ5.1
#=GS W0RNF3_9BACT/832-913        AC W0RNF3.1
#=GS A0A171DER4_9SYNE/836-918    AC A0A171DER4.1
#=GS A0A098S1U6_9BACT/829-910    AC A0A098S1U6.1
#=GS A0A377R0Q9_9NEIS/270-363    AC A0A377R0Q9.1
#=GS A0A1G7EUH7_9ACTO/595-682    AC A0A1G7EUH7.1
#=GS A0A0F5AQ87_9GAMM/837-918    AC A0A0F5AQ87.1
#=GS A0A1Y4TW38_9FIRM/852-935    AC A0A1Y4TW38.1
#=GS G4E8K6_9GAMM/1-68           AC G4E8K6.1
#=GS A0A021VVW6_9CELL/844-927    AC A0A021VVW6.1
#=GS A0A6M5J711_9SPHN/847-928    AC A0A6M5J711.1
#=GS A0A1H3E0N1_ALLWA/508-590    AC A0A1H3E0N1.1
#=GS C8PBJ7_9LACO/818-904        AC C8PBJ7.1
#=GS A7BZ09_9GAMM/627-709        AC A7BZ09.1
#=GS A0A6I3IXD8_9MICO/817-899    AC A0A6I3IXD8.1
#=GS A0A0C9NA46_9FUNG/171-253    AC A0A0C9NA46.1
#=GS S1QW52_9ENTE/826-905        AC S1QW52.1
#=GS A0A495BMN1_9BACT/830-910    AC A0A495BMN1.1
#=GS A0A126RHI6_9SPHN/833-915    AC A0A126RHI6.1
#=GS A0A1M4MY96_9RHOB/810-892    AC A0A1M4MY96.1
#=GS A0A1H9HX15_9RHOB/829-911    AC A0A1H9HX15.1
#=GS A0A239DID9_9SPHN/833-914    AC A0A239DID9.1
#=GS A0A1Y6D4N2_9GAMM/668-750    AC A0A1Y6D4N2.1
#=GS A0A142LKB7_9PROT/833-914    AC A0A142LKB7.1
#=GS A0A6J4FMZ4_9PROT/833-915    AC A0A6J4FMZ4.1
#=GS A0A023D765_ACIMT/825-906    AC A0A023D765.1
#=GS A0A4P6UZ58_9RHIZ/826-911    AC A0A4P6UZ58.1
#=GS A0A1H4HG81_9SPHI/823-904    AC A0A1H4HG81.1
#=GS A0A345QCU7_9RHOB/810-894    AC A0A345QCU7.1
#=GS A0A369US98_9GAMM/832-914    AC A0A369US98.1
#=GS K0YMU4_9ACTN/342-421        AC K0YMU4.1
#=GS A0A5C6HLT6_9BACT/810-890    AC A0A5C6HLT6.1
#=GS A0A239I2N6_9FIRM/796-876    AC A0A239I2N6.1
#=GS A0A3A5MRP7_9MICO/835-918    AC A0A3A5MRP7.1
#=GS A0A4R1LZV2_9SPHI/798-879    AC A0A4R1LZV2.1
#=GS A0A316J4V5_9RHIZ/845-926    AC A0A316J4V5.1
#=GS A0A2P7TND3_9SPHI/830-914    AC A0A2P7TND3.1
#=GS R5ZT04_9BACT/830-910        AC R5ZT04.1
#=GS A0A0M1J5W1_9GAMM/459-541    AC A0A0M1J5W1.1
#=GS A0A2A3YCS9_9MICO/810-888    AC A0A2A3YCS9.1
#=GS L1MEK9_9CORY/804-886        AC L1MEK9.1
#=GS A0A370G670_9BACI/807-887    AC A0A370G670.1
#=GS J0DF14_9BIFI/847-928        AC J0DF14.1
#=GS A0A3A9F6Q7_9BACT/844-927    AC A0A3A9F6Q7.1
#=GS A0A1H3Z7J3_9BACT/881-965    AC A0A1H3Z7J3.1
#=GS A0A366W224_9GAMM/830-910    AC A0A366W224.1
#=GS A0A6L7G4B0_9RHOB/808-890    AC A0A6L7G4B0.1
#=GS A0A0A1YPE4_9PSED/851-933    AC A0A0A1YPE4.1
#=GS A0A1H3ZFI8_9GAMM/838-917    AC A0A1H3ZFI8.1
#=GS F7QPC8_9BRAD/858-940        AC F7QPC8.1
#=GS A0A2T5G0L4_9SPHN/830-911    AC A0A2T5G0L4.1
#=GS A0A2S7XTU3_9GAMM/123-201    AC A0A2S7XTU3.1
#=GS A0A2V5BMY6_9SPHN/834-916    AC A0A2V5BMY6.1
#=GS A0A5Q2RIW3_9ACTN/828-911    AC A0A5Q2RIW3.1
#=GS A0A098SE32_9BACT/834-915    AC A0A098SE32.1
#=GS A0A1B7LVL2_9MICC/850-932    AC A0A1B7LVL2.1
#=GS A0A011SFG6_9LACT/842-922    AC A0A011SFG6.1
#=GS A0A2X0VRJ5_9GAMM/89-170     AC A0A2X0VRJ5.1
#=GS A0A2T4J690_9RHOB/113-195    AC A0A2T4J690.1
#=GS A0A3M0GH30_9FLAO/820-902    AC A0A3M0GH30.1
#=GS E6KUZ3_9ACTO/816-898        AC E6KUZ3.1
#=GS A0A1A6C7Y9_9GAMM/1009-1090  AC A0A1A6C7Y9.1
#=GS A0A447G1M0_9ARCH/817-890    AC A0A447G1M0.1
#=GS K7VU03_9NOST/821-903        AC K7VU03.1
#=GS A0A0M1J3J5_9GAMM/8-90       AC A0A0M1J3J5.1
#=GS A0A399RCY7_9PROT/833-914    AC A0A399RCY7.1
#=GS A0A1E3RY95_9MYCO/77-159     AC A0A1E3RY95.1
#=GS A0A516X9G9_9ACTN/858-940    AC A0A516X9G9.1
#=GS M7MNY6_9MICC/810-892        AC M7MNY6.1
#=GS A0A1E5SZV8_9BACT/833-914    AC A0A1E5SZV8.1
#=GS A0A1H2FWY3_9FLAO/803-885    AC A0A1H2FWY3.1
#=GS A0A560WEG2_9MICO/834-917    AC A0A560WEG2.1
#=GS A0A4R6TZP3_9GAMM/832-914    AC A0A4R6TZP3.1
#=GS A0A0A0EMT7_9GAMM/839-921    AC A0A0A0EMT7.1
#=GS I8HXC3_9GAMM/787-869        AC I8HXC3.1
#=GS A0A2W7NJT4_9BACT/797-878    AC A0A2W7NJT4.1
#=GS A0A494YYA6_9BACI/838-920    AC A0A494YYA6.1
#=GS A0A423PKD0_9GAMM/842-924    AC A0A423PKD0.1
#=GS A0A327JKG2_9RHIZ/833-914    AC A0A327JKG2.1
#=GS A0A1H9VJJ6_9RHOB/837-917    AC A0A1H9VJJ6.1
#=GS U5Q7Z1_9BACT/811-891        AC U5Q7Z1.1
#=GS A0A0G3GRJ2_9CORY/844-924    AC A0A0G3GRJ2.1
#=GS C6XNS2_HIRBI/832-914        AC C6XNS2.1
#=GS A0A286GNU7_9PROT/825-907    AC A0A286GNU7.1
#=GS C0W4W1_9ACTO/853-943        AC C0W4W1.1
#=GS A0A2I1DMU7_9PROT/830-912    AC A0A2I1DMU7.1
#=GS A0A1H6FDI5_9GAMM/877-940    AC A0A1H6FDI5.1
#=GS A0A257HC02_9RHIZ/847-929    AC A0A257HC02.1
#=GS N0DY75_9MICO/840-923        AC N0DY75.1
#=GS A0A1I1P304_9BACT/452-505    AC A0A1I1P304.1
#=GS A0A2C8YI70_9MICO/830-912    AC A0A2C8YI70.1
#=GS A0A1Q8J468_9BURK/853-935    AC A0A1Q8J468.1
#=GS A0A5B7ZSA9_9GAMM/823-914    AC A0A5B7ZSA9.1
#=GS A0A1M5DML0_9BURK/836-918    AC A0A1M5DML0.1
#=GS A0A512H4Q7_9PROT/833-913    AC A0A512H4Q7.1
#=GS A0A3M9N857_9BACT/870-952    AC A0A3M9N857.1
#=GS F0TCX4_9PROT/833-914        AC F0TCX4.1
#=GS A0A3R8PEB9_9CORY/521-595    AC A0A3R8PEB9.1
#=GS A1WIP5_VEREI/835-917        AC A1WIP5.1
#=GS Q3JB44_NITOC/819-900        AC Q3JB44.1
#=GS B1XIC9_SYNP2/821-903        AC B1XIC9.1
#=GS A0A1H3H736_ALLWA/385-466    AC A0A1H3H736.1
#=GS A0A5J5G6J7_9GAMM/812-894    AC A0A5J5G6J7.1
#=GS A0A437N415_9SPHN/833-914    AC A0A437N415.1
#=GS A0A1M5XLI3_9BURK/850-932    AC A0A1M5XLI3.1
#=GS D5X1C9_THIK1/834-916        AC D5X1C9.1
#=GS R2PVM3_9ENTE/779-858        AC R2PVM3.1
#=GS A0A1I3FTR1_9FLAO/821-903    AC A0A1I3FTR1.1
#=GS A0A1R4KGQ4_9MICO/811-893    AC A0A1R4KGQ4.1
#=GS A0A0D7EHK7_RHOPL/835-920    AC A0A0D7EHK7.1
#=GS A0A4Q2RGW4_9RHIZ/824-909    AC A0A4Q2RGW4.1
#=GS A0A1N7LU15_9GAMM/834-915    AC A0A1N7LU15.1
#=GS A0A3S1AHY3_ANAVA/1-53       AC A0A3S1AHY3.1
#=GS A0A425W4A9_9FIRM/865-947    AC A0A425W4A9.1
#=GS A0A0N0J6E6_9RHIZ/825-906    AC A0A0N0J6E6.1
#=GS A0A143X918_9ACTN/842-926    AC A0A143X918.1
#=GS A0A0R1ZJH3_9LACO/803-890    AC A0A0R1ZJH3.1
#=GS K9SSQ6_9SYNE/821-903        AC K9SSQ6.1
#=GS A0A1Y0EE53_9RHOB/808-890    AC A0A1Y0EE53.1
#=GS A0A0F5P833_9SPHN/839-920    AC A0A0F5P833.1
#=GS A0A022KWJ4_9MICO/849-930    AC A0A022KWJ4.1
#=GS A0A1H2H925_9PSED/831-913    AC A0A1H2H925.1
#=GS C4GLG3_9NEIS/844-935        AC C4GLG3.1
#=GS A0A135WBG3_9BACL/824-905    AC A0A135WBG3.1
#=GS K9RTC7_SYNP3/835-920        AC K9RTC7.1
#=GS A0A1G6XC33_9GAMM/823-914    AC A0A1G6XC33.1
#=GS A0A368TP77_9GAMM/827-909    AC A0A368TP77.1
#=GS A0A1D8UYF5_9PROT/803-885    AC A0A1D8UYF5.1
#=GS A0A3B0MD11_9RHOB/833-915    AC A0A3B0MD11.1
#=GS A0A6N9PQA5_9FIRM/465-545    AC A0A6N9PQA5.1
#=GS A0A1Q2MGR8_9BACT/822-904    AC A0A1Q2MGR8.1
#=GS A0A2A2AJE0_9BURK/253-341    AC A0A2A2AJE0.1
#=GS A0A2V5M046_9MICC/808-889    AC A0A2V5M046.1
#=GS B1VE06_CORU7/33-115         AC B1VE06.1
#=GS Q7VCW0_PROMA/833-915        AC Q7VCW0.1
#=GS D5A6G3_ARTPN/188-273        AC D5A6G3.1
#=GS I0IC19_PHYMF/1-49           AC I0IC19.1
#=GS A0A0Q4VBU9_9MICO/811-892    AC A0A0Q4VBU9.1
#=GS A0A0S6WTK8_9SPHN/21-102     AC A0A0S6WTK8.1
#=GS A0A1H6W1T9_9LACT/849-931    AC A0A1H6W1T9.1
#=GS A0A3A9JBJ1_9PROT/833-914    AC A0A3A9JBJ1.1
#=GS A0A418ZVI1_9RHOB/850-932    AC A0A418ZVI1.1
#=GS A0A1L7CEY4_9CORY/812-893    AC A0A1L7CEY4.1
#=GS A0A504J3B8_9FLAO/805-885    AC A0A504J3B8.1
#=GS A0A4U3KZH5_9BACT/806-888    AC A0A4U3KZH5.1
#=GS A0A1S9CIW8_9FIRM/832-914    AC A0A1S9CIW8.1
#=GS A0A1Q8YAK8_9BURK/914-1004   AC A0A1Q8YAK8.1
#=GS S3YGI9_9MICO/313-395        AC S3YGI9.1
#=GS A0A6I0FI71_9CORY/810-892    AC A0A6I0FI71.1
#=GS A0A1G8UY96_9RHOB/807-889    AC A0A1G8UY96.1
#=GS A0A2S1SIR8_9FLAO/821-903    AC A0A2S1SIR8.1
#=GS A0A1Y3XEL1_9FIRM/837-919    AC A0A1Y3XEL1.1
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