#=GF ID NMO
#=GF AC PF03060.18
#=GF DE Nitronate monooxygenase
#=GF PI NPD;
#=GF AU Griffiths-Jones SR;0000-0001-6043-807X
#=GF AU Bateman A;0000-0002-6982-4660
#=GF SE Pfam-B_2634 (release 6.4)
#=GF GA 28.00 28.00;
#=GF TC 28.00 28.00;
#=GF NC 27.90 27.90;
#=GF BM hmmbuild HMM.ann SEED.ann
#=GF SM hmmsearch -Z 61295632 -E 1000 --cpu 4 HMM pfamseq
#=GF TP Domain
#=GF CL CL0036
#=GF RN [1]
#=GF RM 9501443
#=GF RT Purification, characterization, and mechanism of a flavin
#=GF RT mononucleotide-dependent 2-nitropropane dioxygenase from
#=GF RT Neurospora crassa.
#=GF RA Gorlatova N, Tchorzewski M, Kurihara T, Soda K, Esaki N;
#=GF RL Appl Environ Microbiol 1998;64:1029-1033.
#=GF RN [2]
#=GF RM 15582992
#=GF RT Involvement of a flavosemiquinone in the enzymatic oxidation of
#=GF RT nitroalkanes catalyzed by 2-nitropropane dioxygenase.
#=GF RA Francis K, Russell B, Gadda G;
#=GF RL J Biol Chem. 2005;280:5195-5204.
#=GF RN [3]
#=GF RM 16682407
#=GF RT Crystal structure of 2-nitropropane dioxygenase complexed with
#=GF RT FMN and substrate. Identification of the catalytic base.
#=GF RA Ha JY, Min JY, Lee SK, Kim HS, Kim do J, Kim KH, Lee HH, Kim HK,
#=GF RA Yoon HJ, Suh SW;
#=GF RL J Biol Chem. 2006;281:18660-18667.
#=GF RN [4]
#=GF RM 19577534
#=GF RT Nitronate monooxygenase, a model for anionic flavin semiquinone
#=GF RT intermediates in oxidative catalysis.
#=GF RA Gadda G, Francis K;
#=GF RL Arch Biochem Biophys. 2010;493:53-61.
#=GF RN [5]
#=GF RM 19683782
#=GF RT Kinetic evidence for an anion binding pocket in the active site
#=GF RT of nitronate monooxygenase.
#=GF RA Francis K, Gadda G;
#=GF RL Bioorg Chem. 2009;37:167-172.
#=GF DR INTERPRO; IPR004136;
#=GF DR SO; 0000417; polypeptide_domain;
#=GF CC Nitronate monooxygenase (NMO), formerly referred to as
#=GF CC 2-nitropropane dioxygenase (NPD) (EC:1.13.11.32), is an
#=GF CC FMN-dependent enzyme that uses molecular oxygen to oxidize
#=GF CC (anionic) alkyl nitronates and, in the case of the enzyme from
#=GF CC Neurospora crassa, (neutral) nitroalkanes to the corresponding
#=GF CC carbonyl compounds and nitrite. Previously classified as
#=GF CC 2-nitropropane dioxygenase [1,2,3], but it is now recognized
#=GF CC that this was the result of the slow ionization of nitroalkanes
#=GF CC to their nitronate (anionic) forms [4]. The enzymes from the
#=GF CC fungus Neurospora crassa and the yeast Williopsis saturnus var.
#=GF CC mrakii (formerly classified as Hansenula mrakii) contain
#=GF CC non-covalently bound FMN as the cofactor. Active towards linear
#=GF CC alkyl nitronates of lengths between 2 and 6 carbon atoms and,
#=GF CC with lower activity, towards propyl-2-nitronate. The enzyme from
#=GF CC N. crassa can also utilize neutral nitroalkanes, but with lower
#=GF CC activity. One atom of oxygen is incorporated into the carbonyl
#=GF CC group of the aldehyde product. The reaction appears to involve
#=GF CC the formation of an enzyme-bound nitronate radical and an
#=GF CC a-peroxynitroethane species, which then decomposes, either in
#=GF CC the active site of the enzyme or after release, to acetaldehyde
#=GF CC and nitrite.
#=GF SQ 19058
#=GS A1WJN3_VEREI/28-329 AC A1WJN3.1
#=GS A0A0Q6VS71_9BURK/4-307 AC A0A0Q6VS71.1
#=GS T1I102_RHOPR/1-72 AC T1I102.1
#=GS A0A166R3L1_9ACTN/91-231 AC A0A166R3L1.1
#=GS A0A1L6I5V1_9BURK/7-353 AC A0A1L6I5V1.1
#=GS B7G6R5_PHATC/58-322 AC B7G6R5.1
#=GS IPDC_RHOJR/79-340 AC Q0S7Q1.1
#=GS A0A2S6FXE9_9CLOT/5-352 AC A0A2S6FXE9.1
#=GS A0A1C6MRB2_9NEIS/12-308 AC A0A1C6MRB2.1
#=GS A0A158HE08_9BURK/6-375 AC A0A158HE08.1
#=GS A0A4R3KCV5_9FIRM/2-83 AC A0A4R3KCV5.1
#=GS A3CQ58_STRSV/2-305 AC A3CQ58.1
#=GS A0A371SNE6_9BACI/4-309 AC A0A371SNE6.1
#=GS A0A511Z935_9BACL/4-79 AC A0A511Z935.1
#=GS A0A2N5ML51_9BACI/8-312 AC A0A2N5ML51.1
#=GS A0A5N6FJ29_PETAA/4-312 AC A0A5N6FJ29.1
#=GS A1UM47_MYCSK/2-359 AC A1UM47.1
#=GS D7C593_STRBB/2-345 AC D7C593.1
#=GS A0A231H112_9NOCA/10-359 AC A0A231H112.1
#=GS A0A1U7EAI8_9LACT/75-305 AC A0A1U7EAI8.1
#=GS A0A4S5EQE8_9ACTN/125-414 AC A0A4S5EQE8.1
#=GS A0A4Q5NX27_9BRAD/3-309 AC A0A4Q5NX27.1
#=GS A0A0Q4XWL2_9BURK/68-306 AC A0A0Q4XWL2.1
#=GS A0A017RZE0_9CLOT/2-300 AC A0A017RZE0.1
#=GS A0A0R2D245_9LACO/1-79 AC A0A0R2D245.1
#=GS A0A5N5DP17_9PEZI/121-343 AC A0A5N5DP17.1
#=GS A0A4S8RT39_9FLAO/4-307 AC A0A4S8RT39.1
#=GS A0A5C8NHL8_9ACTN/1-340 AC A0A5C8NHL8.1
#=GS A0A1M6UUD2_9FIRM/3-348 AC A0A1M6UUD2.1
#=GS A0A0Q7ZF79_9ACTN/73-294 AC A0A0Q7ZF79.1
#=GS A0A5R9M4Y0_9ACTN/2-345 AC A0A5R9M4Y0.1
#=GS A0A0L6X0L6_9AGAR/3-311 AC A0A0L6X0L6.1
#=GS D9Y1Q9_9ACTN/34-374 AC D9Y1Q9.1
#=GS J2KV83_9BURK/7-310 AC J2KV83.1
#=GS A0A2J6QIJ0_9HELO/108-351 AC A0A2J6QIJ0.1
#=GS A0A6C2D334_9RHOO/116-309 AC A0A6C2D334.1
#=GS A0A3M0CKV3_9PROT/2-310 AC A0A3M0CKV3.1
#=GS A0A3B7ADG2_9RHOO/9-309 AC A0A3B7ADG2.1
#=GS A0A427YUY3_9TREE/3-315 AC A0A427YUY3.1
#=GS A0A5C8NRX9_9BURK/5-64 AC A0A5C8NRX9.1
#=GS A0A0A6Q8E6_9BURK/9-307 AC A0A0A6Q8E6.1
#=GS G6Y3F2_9RHIZ/10-244 AC G6Y3F2.1
#=GS A0A6N6VLB7_9RHIZ/5-347 AC A0A6N6VLB7.1
#=GS A0A3N2DK96_9GAMM/3-342 AC A0A3N2DK96.1
#=GS A0A0D2CRU6_9EURO/4-339 AC A0A0D2CRU6.1
#=GS A0A2C5XLS1_9PEZI/8-341 AC A0A2C5XLS1.1
#=GS K7L7D2_SOYBN/69-320 AC K7L7D2.1
#=GS A0A2N5MGM9_9BACI/6-307 AC A0A2N5MGM9.1
#=GS L1LSD4_PSEPU/64-301 AC L1LSD4.1
#=GS A0A399RJ21_9PROT/2-253 AC A0A399RJ21.1
#=GS A0A150VIW0_9PEZI/115-253 AC A0A150VIW0.1
#=GS A0A1E3B143_9EURO/12-78 AC A0A1E3B143.1
#=GS A0A5N7DQE4_9EURO/4-298 AC A0A5N7DQE4.1
#=GS F2BYA0_9FIRM/79-303 AC F2BYA0.1
#=GS A0A4P9W0N5_9FUNG/25-93 AC A0A4P9W0N5.1
#=GS A0A1C4QH40_9ACTN/4-76 AC A0A1C4QH40.1
#=GS A0A501XIP4_9SPHN/96-326 AC A0A501XIP4.1
#=GS A5I7Y0_CLOBH/4-351 AC A5I7Y0.1
#=GS A0A6N9YSP2_9ACTN/1-344 AC A0A6N9YSP2.1
#=GS A0A127FDB0_STEDE/2-310 AC A0A127FDB0.1
#=GS Q04DM9_OENOB/4-308 AC Q04DM9.1
#=GS A0A1H8Y127_9PSEU/2-337 AC A0A1H8Y127.1
#=GS A0A7G3URF6_STRT9/2-337 AC A0A7G3URF6.1
#=GS A0A173L0N7_9SPHN/2-309 AC A0A173L0N7.1
#=GS G7VAD6_THELD/75-302 AC G7VAD6.1
#=GS A0A248JPP2_9PROT/6-350 AC A0A248JPP2.1
#=GS S2K2Y6_MUCC1/4-311 AC S2K2Y6.1
#=GS A0A257EQZ4_9PROT/13-470 AC A0A257EQZ4.1
#=GS A0A516SHL3_9BURK/2-100 AC A0A516SHL3.1
#=GS A0A068RUF7_9FUNG/4-308 AC A0A068RUF7.1
#=GS A0A1H9NWY3_9BACI/5-310 AC A0A1H9NWY3.1
#=GS A0A1Y2DUX3_9PEZI/8-358 AC A0A1Y2DUX3.1
#=GS A0A2C1YT16_9BACI/2-303 AC A0A2C1YT16.1
#=GS A0A346YIM8_9RHIZ/5-308 AC A0A346YIM8.1
#=GS A0A0D2F4L4_9EURO/8-331 AC A0A0D2F4L4.1
#=GS A0A1Y2BTI8_9FUNG/7-318 AC A0A1Y2BTI8.1
#=GS Q899P2_CLOTE/10-307 AC Q899P2.1
#=GS A0A1J9QVH1_9PEZI/8-331 AC A0A1J9QVH1.1
#=GS A0A0A2K6L2_PENEN/122-337 AC A0A0A2K6L2.1
#=GS A0A1Q9PZV0_9BACI/71-312 AC A0A1Q9PZV0.1
#=GS K6W4I1_9ACTN/1-176 AC K6W4I1.1
#=GS A0A1H3WZG6_9BACT/72-302 AC A0A1H3WZG6.1
#=GS A0A4S3JMF4_9EURO/4-334 AC A0A4S3JMF4.1
#=GS A0A5P8WDZ8_9NOSO/103-396 AC A0A5P8WDZ8.1
#=GS A0A6H0Y2T0_9PEZI/68-326 AC A0A6H0Y2T0.1
#=GS A0A221STJ0_9DEIO/226-356 AC A0A221STJ0.1
#=GS A0A7H9BG16_9NEIS/14-319 AC A0A7H9BG16.1
#=GS A0A4R1URH0_9SPHN/85-328 AC A0A4R1URH0.1
#=GS A0A1G9BYB5_9CLOT/75-301 AC A0A1G9BYB5.1
#=GS R6AT14_9PROT/12-350 AC R6AT14.1
#=GS A0A6I3L5Q2_9NOCA/10-359 AC A0A6I3L5Q2.1
#=GS A0A0C2W2N8_9BACL/3-350 AC A0A0C2W2N8.1
#=GS L0NBV8_9RHIZ/4-308 AC L0NBV8.1
#=GS A0A6H2DME5_9SPHN/86-328 AC A0A6H2DME5.1
#=GS A0A542DDK5_9PSEU/5-83 AC A0A542DDK5.1
#=GS A0A0C5G640_9ACTN/695-958 AC A0A0C5G640.1
#=GS A0A1H0DV73_9BACI/5-325 AC A0A1H0DV73.1
#=GS A0A097EC76_9SPHN/6-306 AC A0A097EC76.1
#=GS V8D4Q2_9NOCA/5-339 AC V8D4Q2.1
#=GS A0A5M3XB15_9ACTN/1-333 AC A0A5M3XB15.1
#=GS A0A1H2ZJ20_9FLAO/3-316 AC A0A1H2ZJ20.1
#=GS A0A1G6IH19_9GAMM/6-310 AC A0A1G6IH19.1
#=GS A5V7X4_SPHWW/22-328 AC A5V7X4.1
#=GS A0A1I6JTU0_9SPHN/13-333 AC A0A1I6JTU0.1
#=GS A0A239E022_9PROT/61-309 AC A0A239E022.1
#=GS A0A371SK31_9BACI/3-351 AC A0A371SK31.1
#=GS N1MS53_9SPHN/11-94 AC N1MS53.1
#=GS A0A1I4X0C2_9PSED/9-352 AC A0A1I4X0C2.1
#=GS A0A1H5B6M7_9ACTN/2-343 AC A0A1H5B6M7.1
#=GS U1ZR54_9BURK/6-350 AC U1ZR54.1
#=GS A0A1S1QC23_9ACTN/70-309 AC A0A1S1QC23.1
#=GS A0A268E8X0_9BACI/3-351 AC A0A268E8X0.1
#=GS A0A2K2G212_9SPHN/75-301 AC A0A2K2G212.1
#=GS K6DJP2_BACAZ/4-79 AC K6DJP2.1
#=GS G1XGK5_ARTOA/6-329 AC G1XGK5.1
#=GS U4V082_9RHOB/54-308 AC U4V082.1
#=GS Q12H21_POLSJ/91-335 AC Q12H21.1
#=GS A0A199XS57_9FLAO/3-316 AC A0A199XS57.1
#=GS A0A2S6NBL1_RHOGL/98-469 AC A0A2S6NBL1.1
#=GS A0A2I0AYB9_9ASPA/3-79 AC A0A2I0AYB9.1
#=GS A0A328AIH4_9CAUL/4-344 AC A0A328AIH4.1
#=GS A0A1Y1T2W2_9FLAO/3-303 AC A0A1Y1T2W2.1
#=GS A0A1B8EPU7_9PEZI/8-340 AC A0A1B8EPU7.1
#=GS A0A067NTJ0_PLEOS/6-332 AC A0A067NTJ0.1
#=GS R5PN16_9BACT/15-315 AC R5PN16.1
#=GS A0A519ZMK1_9BURK/2-345 AC A0A519ZMK1.1
#=GS A0A1T4YU18_9ACTN/4-298 AC A0A1T4YU18.1
#=GS A0A0U3BE30_9ALTE/8-307 AC A0A0U3BE30.1
#=GS A0A365L1T4_9BACL/4-78 AC A0A365L1T4.1
#=GS A1WKR8_VEREI/39-281 AC A1WKR8.1
#=GS A0A3L7K5B4_9BACI/74-309 AC A0A3L7K5B4.1
#=GS A0A2V5HN87_ASPV1/8-340 AC A0A2V5HN87.1
#=GS A0A069DPL4_9BACL/4-319 AC A0A069DPL4.1
#=GS A0A516KJI9_9BACI/4-351 AC A0A516KJI9.1
#=GS A0A496PF99_9MICC/8-343 AC A0A496PF99.1
#=GS A0A4R2PQM6_9SPHN/6-313 AC A0A4R2PQM6.1
#=GS A0A1M7HZ25_9RHOB/57-311 AC A0A1M7HZ25.1
#=GS F6EHB7_HOYSD/3-309 AC F6EHB7.1
#=GS A0A3N4V672_9BURK/4-308 AC A0A3N4V672.1
#=GS A0A0L8ERJ0_9BURK/43-309 AC A0A0L8ERJ0.1
#=GS A0A2I0K477_PUNGR/4-76 AC A0A2I0K477.1
#=GS A0A1L9V5E9_ASPGL/5-340 AC A0A1L9V5E9.1
#=GS A0A418Y0N2_9BURK/2-310 AC A0A418Y0N2.1
#=GS A0A1B6Z701_9SPHN/79-303 AC A0A1B6Z701.1
#=GS A0A4Y9AFB9_9BACI/4-79 AC A0A4Y9AFB9.1
#=GS A0A1H3HEX7_9PSEU/2-337 AC A0A1H3HEX7.1
#=GS A0A2S0MUU9_9RHOB/15-322 AC A0A2S0MUU9.1
#=GS A0A1I4XIH7_9PSEU/3-361 AC A0A1I4XIH7.1
#=GS A0A2S7WVJ5_9FLAO/73-302 AC A0A2S7WVJ5.1
#=GS A0A6P1IVA7_9BURK/6-305 AC A0A6P1IVA7.1
#=GS A0A5S9PSL4_9GAMM/11-361 AC A0A5S9PSL4.1
#=GS A0A5M3MRU4_CONPW/3-311 AC A0A5M3MRU4.1
#=GS A0A402BUX0_9FIRM/2-81 AC A0A402BUX0.1
#=GS A0A4V6KEU6_HATHI/3-301 AC A0A4V6KEU6.1
#=GS A0A559UU24_9ACTN/13-351 AC A0A559UU24.1
#=GS A0A0P0MFZ6_9BURK/2-81 AC A0A0P0MFZ6.1
#=GS A0A423PW20_9GAMM/1-340 AC A0A423PW20.1
#=GS A6VPW3_ACTSZ/2-89 AC A6VPW3.1
#=GS A0A386DTB7_9RHIZ/5-308 AC A0A386DTB7.1
#=GS A0A1H5UXB5_9BACT/7-353 AC A0A1H5UXB5.1
#=GS L7KG44_9ACTN/7-368 AC L7KG44.1
#=GS A0A1I0CGL5_9RHOB/3-343 AC A0A1I0CGL5.1
#=GS A0A177P6I1_9RHIZ/57-205 AC A0A177P6I1.1
#=GS A0A0B4S2J5_9FIRM/72-298 AC A0A0B4S2J5.1
#=GS A0A075KC23_9FIRM/5-310 AC A0A075KC23.1
#=GS A0A2J6QHX6_9HELO/128-350 AC A0A2J6QHX6.1
#=GS A0A163DLE3_DIDRA/7-330 AC A0A163DLE3.1
#=GS A0A0P7AWE3_9HYPO/8-332 AC A0A0P7AWE3.1
#=GS A0A2N0EUF8_9FLAO/73-302 AC A0A2N0EUF8.1
#=GS A0A1Q3H3Y4_9BACT/2-310 AC A0A1Q3H3Y4.1
#=GS 2NDP_YEAST/25-396 AC P47177.2
#=GS A0A437M8S1_9SPHN/1-345 AC A0A437M8S1.1
#=GS A0A248YL13_9ACTN/6-283 AC A0A248YL13.1
#=GS A0A1G9I4G4_9PSED/2-348 AC A0A1G9I4G4.1
#=GS R6CNM0_9FIRM/5-304 AC R6CNM0.1
#=GS A0A6A6YFC8_9PEZI/4-339 AC A0A6A6YFC8.1
#=GS A0A0E9MYU5_9BACT/1-345 AC A0A0E9MYU5.1
#=GS C1GS26_PARBA/21-353 AC C1GS26.2
#=GS A0A1S3C1I8_CUCME/72-320 AC A0A1S3C1I8.1
#=GS A0A2I0QU66_9BACI/3-306 AC A0A2I0QU66.1
#=GS A0A0U1M781_TALIS/8-340 AC A0A0U1M781.1
#=GS B6YQY3_AZOPC/2-348 AC B6YQY3.1
#=GS A0A067Q608_9AGAM/100-338 AC A0A067Q608.1
#=GS A0A1H8LKA3_9PROT/84-308 AC A0A1H8LKA3.1
#=GS A0A3P4B9V4_9BURK/54-284 AC A0A3P4B9V4.1
#=GS A0A2R3QBP7_9BURK/4-347 AC A0A2R3QBP7.1
#=GS A0A3S2UL60_9SPHI/3-346 AC A0A3S2UL60.1
#=GS A0A4R6WWE8_9PROT/78-325 AC A0A4R6WWE8.1
#=GS Q0AVV2_SYNWW/65-311 AC Q0AVV2.1
#=GS A0A5N6KLH3_9HELO/121-341 AC A0A5N6KLH3.1
#=GS U1KPP7_9GAMM/42-331 AC U1KPP7.1
#=GS K6G5L1_9GAMM/2-310 AC K6G5L1.1
#=GS A0A0C3D2M3_9PEZI/18-346 AC A0A0C3D2M3.1
#=GS A0A370HP61_9RHIZ/4-348 AC A0A370HP61.1
#=GS A0A1R1S9U1_9ACTN/10-345 AC A0A1R1S9U1.1
#=GS A0A1V8TBT2_9PEZI/112-262 AC A0A1V8TBT2.1
#=GS F1W3G4_9BURK/1-344 AC F1W3G4.1
#=GS A0A0Q5DMP2_9BURK/13-292 AC A0A0Q5DMP2.1
#=GS A0A150LQQ4_9BACI/13-89 AC A0A150LQQ4.1
#=GS A0A5Q0K273_9FLAO/2-302 AC A0A5Q0K273.1
#=GS A0A2P1NK95_9BURK/3-307 AC A0A2P1NK95.1
#=GS F5L9G3_CALTT/74-315 AC F5L9G3.1
#=GS A0A0Q2V505_9RHOB/12-346 AC A0A0Q2V505.1
#=GS A0A0A0DKU4_9BURK/3-309 AC A0A0A0DKU4.1
#=GS A0A1L8RHT6_9ENTE/66-179 AC A0A1L8RHT6.1
#=GS A0A1Y1XK82_9FUNG/91-316 AC A0A1Y1XK82.1
#=GS A0A3N4V2M3_9ACTN/14-310 AC A0A3N4V2M3.1
#=GS A0A6V8KTB8_9ACTN/7-319 AC A0A6V8KTB8.1
#=GS Q0RXY5_RHOJR/2-334 AC Q0RXY5.1
#=GS A0A093YQ82_9PEZI/5-340 AC A0A093YQ82.1
#=GS A0A4U6XI88_9PEZI/8-344 AC A0A4U6XI88.1
#=GS R5ZI46_9FIRM/201-388 AC R5ZI46.1
#=GS R4KAC5_CLOPA/5-352 AC R4KAC5.1
#=GS A0A5N6UQF6_9EURO/4-328 AC A0A5N6UQF6.1
#=GS A0A3R9YDT7_9ENTE/78-307 AC A0A3R9YDT7.1
#=GS A0A0U3MQR2_9BURK/25-368 AC A0A0U3MQR2.1
#=GS Q0CLM2_ASPTN/4-259 AC Q0CLM2.1
#=GS A0A4P6TUQ7_STRSO/2-343 AC A0A4P6TUQ7.1
#=GS I3ZXC0_ORNRL/73-301 AC I3ZXC0.1
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#=GS A0A239ATN7_9FLAO/2-81 AC A0A239ATN7.1
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#=GS Q9WZQ7_THEMA/4-85 DR PDB; 3BO9 A; 75-300;
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#=GS Q9WZQ7_THEMA/4-85 DR PDB; 5GVJ B; 75-300;
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#=GS Q7MAW0_PORGI/73-301 DR PDB; 4IQL B; 73-301;
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