#=GF ID   RE_ScaI
#=GF AC   PF09569.16
#=GF DE   ScaI restriction endonuclease
#=GF AU   Coggill P;0000-0001-5731-1588
#=GF SE   Bateman A
#=GF GA   25.00 25.00;
#=GF TC   31.90 31.90;
#=GF NC   22.00 20.80;
#=GF BM   hmmbuild  HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 90746521 --cpu 8 -E 1000 HMM pfamseq
#=GF TP   Family
#=GF CL   CL0236
#=GF RN   [1]
#=GF RM   9862476
#=GF RT   Cloning and expression of the ApaLI, NspI, NspHI, SacI, ScaI,
#=GF RT   and SapI restriction-modification systems in Escherichia coli. 
#=GF RA   Xu SY, Xiao JP, Ettwiller L, Holden M, Aliotta J, Poh CL, Dalton
#=GF RA   M, Robinson DP, Petronzio TR, Moran L, Ganatra M, Ware J, Slatko
#=GF RA   B, Benner J; 
#=GF RL   Mol Gen Genet. 1998;260:226-231.
#=GF RN   [2]
#=GF RM   22638584
#=GF RT   Sequence, structure and functional diversity of PD-(D/E)XK
#=GF RT   phosphodiesterase superfamily.
#=GF RA   Steczkiewicz K, Muszewska A, Knizewski L, Rychlewski L, Ginalski
#=GF RA   K;
#=GF RL   Nucleic Acids Res. 2012;40:7016-7045.
#=GF DR   INTERPRO; IPR019069;
#=GF DR   SO; 0100021; polypeptide_conserved_region;
#=GF CC   This family includes the ScaI (recognises and cleaves AGT^ACT)
#=GF CC   restriction endonuclease.
#=GF SQ   24
#=GS A0A7W6CNW3_9HYPH/4-194   AC A0A7W6CNW3.1
#=GS A0A917D2B6_9BACL/1-174   AC A0A917D2B6.1
#=GS A0A927AP40_9CYAN/3-193   AC A0A927AP40.1
#=GS A0A0F5YM08_9CYAN/1-180   AC A0A0F5YM08.1
#=GS A0A1Z4BVI4_9GAMM/5-196   AC A0A1Z4BVI4.1
#=GS A0A8D5JX30_9PROT/2-191   AC A0A8D5JX30.1
#=GS A0A1M4VPM4_9BURK/3-191   AC A0A1M4VPM4.1
#=GS A0A160T471_9CHLR/3-193   AC A0A160T471.2
#=GS B7K946_GLOC7/2-54        AC B7K946.1
#=GS A0A5J4FEU3_MICAE/3-193   AC A0A5J4FEU3.1
#=GS A0A2G8RF53_9RHOB/1-173   AC A0A2G8RF53.1
#=GS E6SPG6_BACT6/3-192       AC E6SPG6.1
#=GS A0A2K8UHG8_9GAMM/3-194   AC A0A2K8UHG8.1
#=GS A0A1J1LN27_9CYAN/3-42    AC A0A1J1LN27.1
#=GS A0A0V8JK51_9BACI/3-197   AC A0A0V8JK51.1
#=GS A0A926YBP6_9CYAN/3-193   AC A0A926YBP6.1
#=GS A0AB33BWW4_MICA7/3-193   AC A0AB33BWW4.1
#=GS A0YQ31_LYNSP/4-194       AC A0YQ31.1
#=GS A0A261KN56_9CYAN/3-182   AC A0A261KN56.1
#=GS A0A073CGK1_PLAA1/3-192   AC A0A073CGK1.1
#=GS A0A1G6P910_9BURK/3-191   AC A0A1G6P910.1
#=GS A0A1J1LLZ0_9CYAN/1-46    AC A0A1J1LLZ0.1
#=GS A0A939S6C9_9MICO/7-195   AC A0A939S6C9.1
#=GS A0A6I4NV34_9MICO/11-201  AC A0A6I4NV34.1
A0A7W6CNW3_9HYPH/4-194              ..SPYAGHPREEWPEITRRLVDVFPLQPDQLLQFVDSAWSNLFNSSF..GEsgL..KIG...TDILL...PAQATGVLLERLIAVEMQKSL-EGWRGGRTKTEKDIICETDLKFSFEIKTSSSRS.GLYGNRSTGHRAEGRT.--KFRSGYYLVINYKLP...TEDNSSREIINVRFGWIDDDDWVGQAQPTGQQASIGVGLARLKLITI-r...............
A0A917D2B6_9BACL/1-174              .m-------------------NSHPLKPEDIVEAVLESWEGILTTKI.aGE..L..QLG...VDVFP...VPQIMGSFLHELIPVKLSKKYPASWRRERDKGDKDLVYIPEDFYSIEIKTSSNKT.NIFGNRSYGQENSENN.SGKSKSGFYITINFEKF...EDD-IIPEITKVRFGWIDHSDWKAQKSATGQQASLSKEARDYKLLLLY................
A0A927AP40_9CYAN/3-193              ..SPYLGRPSHEWFSITQTLVSNHPLEPQEIVDTVLFCWQSILETKL..GSrnF..QIG...KDIFP...KPQIMGFFLHELIPLELEAKYPGFWRGERTAFDKDIVYLPDDSFSIEVKTSSAPN.RIYGNRSYAQETGRDK.--KRKSGYYLAINFEKF...SISR-MPSIRLIRFGWIDSSDWIAQKAATGQQSRLAVEVEKYKLLELY................
A0A0F5YM08_9CYAN/1-180              ma-------------ITRQVIDEFPLSSEVLISTVEEAWKDLYSSSF..GD..FrlQIG...RDIFL...PAQAIGIILERLIAVRLAHQN-SGWRGSQAKHEKDIVCTFNDRYSFEVKTSSSKS.SFYGNRSTGHRSENRL.--KYRTGYYLIINYKLP...KEDDLQRKIWKIRFGWIDDEDWVGQNKPTGQQASIGAKVASLKLVTL-k...............
A0A1Z4BVI4_9GAMM/5-196              ..SPYAQSAKSDWLQITHDLIANHPLDEQEIVGFCFAAWQGLFSSTI..GRhrL..KIG...EHIFP...KPQVIGALLHELIPAEIAASYPGIWQREQEKYDKDIVYLPDDRYSIELKTSSNPN.QIFANRSYAQTPSQQK.--KSKDGYYLAVNFEQI...KAKTQQPRILMIRFGWLDHTDWIGQASETGQQAKLAPETYRLKLKTLY................
A0A8D5JX30_9PROT/2-191              ..SPYATASIDQWPDITNQLISDHPLHPAEILDIAQVCWESVWETQI..GS..G..STIinlIEISP...PATVIGYFFEKLFAKELARRYPAEWRGGVTGNDKDLVCIADNQKSIEIKASGQLGlKIYGNRSYGQEAENAHlVKKDKSGYYITVNFY--...-----ANVLTLVRFGWIDASDWQAQKAATGQMAGLNPNVYEYKLLPL-k...............
A0A1M4VPM4_9BURK/3-191              ..SPYLGAPVQQWAGITQQLVQQHPLTPDLILDAAMLSWTRLWNTWV..GD..T..AVG...FPIAEidpPATVIGYMFEKLFAKELAVRLPGAWRGGVG-SEKDLHCIQNDSLSVEMKASGQLGyKIYGNRSYGQVLENADaAKKDKSGYYITVNFY--...-----GQTLTLLRFGWIDSSDWQAQKSPTGQMAGLPPEVYLHKLMPI-v...............
A0A160T471_9CHLR/3-193              ..SPYHELPIEMWVTRTRELIDAHPLDPNEIYEVVLTVWDDIFNSKI..GSkpF..KIG...QDLFP...RPQIMAYFLHELIPLEFSSRYPGVWRREETADEKDLVYIPDPNYSIEIKTSSSAK.RIFGNRSYTQEAENSK.--KSKSGYYLAINFQRF...SSS-QKPRIIGVRFGWIDHFDWIGQATATGQQARLDLASERYKLLN--lp..............
B7K946_GLOC7/2-54                   ..SPYDNIPQHEWSAKTRELIERHPLQLETIREIALKSWDVLWQTTI..GE..G..Q--...-----...-------------------------------------------------------.----------------.-----------------...------------------------------------------------tdpn............
A0A5J4FEU3_MICAE/3-193              ..SPYADVSPSNWLSITQRLVERHPLSTDAIVDVVLTSWQSIFNSQL..GTkgF..RIG...RDIFP...KPQIMGFLLHELIPLELKAKSPDLWRGDISISDKDIVYIPDDFFSIEVKTSSDPR.HIYGNRSYAQNSNN--.SKKGKSGYYLAVNFEKF...SHTT-NPQIKLIRFGWIDSGDWIGQKAATGQQSRLSSDVENYKLLQLY................
A0A2G8RF53_9RHOB/1-173              ..-----------------MIDEHPLDPDDIVDTVLTCWTAIFESRL.gGK..F..RIG...HDIQP...KPQIMGFLLHELIPLELQSRHAGQWRPEKDKVDKDLVYIPDSGLSVELKTSSHAS.QIFGNRSYAQPQQG--.AGKSKDGYYMTVNFEKF...SKD-GLPKVRLVRFGWLDHTDWIGQAAATGQQARIRPGSDRAKLLRLY................
E6SPG6_BACT6/3-192                  ..SPYNGLNVEDWKSKTEELIKAHPLK-EKIVPIVLKAWDDIFNSQM..GS..F..KIG...KDIFP...SPQIMSFFLHELVAHYLSLEYPKEYKVGEAKKEKDVHNIINPQMGIEIKASSHPT.QIFANRSYAQPSSSSE.-EKDKNGYYIGINFEKF...SESNPKPQILQIRFGYLEHSDWIAQKAATGQQARLSTNADKNKLITIY................
A0A2K8UHG8_9GAMM/3-194              ..SPYHNVSPENWRTVTEKLISKHPLGSKEIVDIVLDAWQSIFTSAI..GShsF..KIG...KDIFP...KPQIMGALLHELIPLEVAARYPKEWRGEQTADDKDIVYVPNDSFSIELKTSSHRD.QIFGNRSYAQDARKDK.--KSKSGYYLTVNFEKF...APQGTEPTILLIRFGWLDHADWIGQRAATGQQSRLTSDIYAGKLKTLY................
A0A1J1LN27_9CYAN/3-42               ..SPYQGLSIEEWTTKTQELIADHPLHLEMIREIALKSY--------..--..-..---...-----...-------------------------------------------------------.----------------.-----------------...------------------------------------------------llk.............
A0A0V8JK51_9BACI/3-197              ..SPYDNLPMEQWINVTEELVGNHPLNNDDIVSIVLEAWDRILDTKI.gGE..L..QIG...VDVFP...GPQIMGNYLHELIPALLSRKFPQYWRKEIIKEDKDVVYIPNNDYSVEIKTSSNPN.NVYGNRSYGQENSENN.SGKSKSGYYITVNFEKFd.vENPANKPEIKKIRFGWIDHTDWKAQKSQTGQAAPISKEARDNKLVLIY................
A0A926YBP6_9CYAN/3-193              ..SPYSGLSVSEWLRVTQEIVERHPLSAEEIVDAVLASWESILDSRL..GTkgF..RIG...KDIFP...KPQIMGFLLHELIPLELAAKHPNNWRGDTSSSDKDLVYIPDDSFSIEVKTSSDPK.HIYGNRSYAQGASKGK.--KGKSGYYLAVNFEKF...SDTRN-SSIRLIRFGWIDSNDWIGQKAATGQQSRLSSDVENYKLLQLY................
A0AB33BWW4_MICA7/3-193              ..SPYADVSPSNWLSITQGLVERHPLSTDAIVDVVLTSWQSIFNSQL..GTkgF..RIG...RDIFP...KPQIMGFLLHELIPLELKAKYPDLWRGDISISDKDIVYIPDDFFSIEVKTSSDPR.HIYGNRSYAQNSNN--.SKKGKSGYYLAVNFEKF...SNTT-NPQIKLIRFGWIDSGDWIGQKAATGQQSRLSSDVENYKLLQLY................
A0YQ31_LYNSP/4-194                  ..SPYRGYQEHEWSAITRQLIEEFPLSSEVIISTVEAAWEDLYSSSF..GD..FrlQIG...RDIFL...PAQAIGVILERLIAVRLAHQN-SGWRGSQTKPEKDIVCTFNERYSFEIKTSSSKN.SFYGNRSTGYRSDNRL.--KSRTGYYLIINYKLP...KEDDLERKIWKIRFGWIDDEDWIGQNKPTGQQASIGTKKAGLKLVTL-k...............
A0A261KN56_9CYAN/3-182              ..SPYAGLPVEAWEAKTLELIKQHPLNYNEIYELINQVWHEIFESNItsSA..Y..KIG...IDLFP...RPQIMGYFLHELIPLELARKYPNIWRREENDNEKYIVCLTNEYYSIEMKTSSSKR.SIYGNRSYAQKSTTNSrLKKNKSGYYLAINFEKFnrnSPASERPKITLVRFGWLDQDDWQGQVAATG------------------................
A0A073CGK1_PLAA1/3-192              ..SPYEGLSIEQWKSKTQELIENHPLHLEMIREIALKSWDILWQTTI..GE..G..ELA...IPLYSldvPAMVVGYFFEKLFAKELQKREPQLWRGGVSKEEKDLVYISDQLYSIEIKTSGQLGlKIFGNRSYGKSVENPDlAKKEKSGYYITVNFY--...-----DRIINLIRFGWIDHSDWKAQSSESGQSAGLSEEIYTYKLIPIA................
A0A1G6P910_9BURK/3-191              ..SPYFGRPVDEWSAITHQLVQSHPLTRDLILEAATLSWTRLWNTWV..GD..A..IVG...FPIAEidpPATVIGYMFEKLFAKELAKRLPGAWRGGIG-SEKDLHCLQDQALSVEMKASGQLGyKIFGNRSYGQVLENADaAKKDKSGYYITVNFY--...-----GQTLTLLRFGWIDASDWQAQKSATGQMAGLPSEVYEHKLLPI-f...............
A0A1J1LLZ0_9CYAN/1-46               .m---------------------------------------------..--..-..---...-----...-------------------REPQVWRGGISKEEKDLVYIPNQFYSILV-------.----------------.-----------------...------------------------------------------------gliiqigkhnyhkqvk
A0A939S6C9_9MICO/7-195              .d-PYAGVDESDWLEVTRQLVDEYPLALPALREVVLDSWSRILTTKI.gGE..L..QIG...DEYKP...SPQMMGNFLHNMIPIVLERNHPSEWRKDDSRDEKDLIYLPDSRFDTEIKTSSS-N.RIFANRSYAQPSAPGS.--KDKSGYYLAINFEAF...KHT-RTPKITMIRLGWLSHRDWIPQTAATGQAAHLAPITYQTKFIS--am..............
A0A6I4NV34_9MICO/11-201             ..SPYFDVPDNERQAVTRELLREHPLSGQDVTNAVLDAWEDIFTSTI..GV..G..RIG...IDIFP...TPQIMGFLLHELIPLRVSSSH-NGWRKDSEASEKDLVFEPDLRFSTEVKTSSNSN.LIYGNRSYGVENSGRG.-KKAKSGYYIAVNFQSW..rEAGRNQPRIRRVRFGWIDSTDWIAQTAETGQASSLPSIVYGSQL----aivf............
#=GC seq_cons                       ..SPYtshs.ppW.slTppLlppHPLss-tIl-hVLpuWpslasopl..Gp..h..pIG...hDIaP....sQlhGhhLccLIshcLtt+hPshWRsp.sts-KDlVhlssstaSlElKTSSp.s.pIaGNRSYuQpspstp...KsKSGYYlslNFch....p.s..tspIhhlRFGWIDpsDWhuQpusTGQtupLssps.phKLl.l.................
//