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Database: Pfam
Entry: TbpB_A
LinkDB: TbpB_A
Original site: TbpB_A 
#=GF ID   TbpB_A
#=GF AC   PF17484.4
#=GF DE   N-Lobe handle Tf-binding protein B
#=GF PI   Domain_A_TbpB;
#=GF AU   Finn RD;0000-0001-8626-2148
#=GF AU   Bateman A;0000-0002-6982-4660
#=GF AU   El-Gebali S;0000-0003-1378-5495
#=GF SE   Pfam-B_893 (release 3.0)
#=GF GA   25.00 25.00;
#=GF TC   27.80 27.80;
#=GF NC   24.40 24.20;
#=GF BM   hmmbuild --amino HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Domain
#=GF RN   [1]
#=GF RM   18186471
#=GF RT   The solution structure of the outer membrane lipoprotein OmlA
#=GF RT   from Xanthomonas axonopodis pv. citri reveals a protein fold
#=GF RT   implicated in protein-protein interaction.
#=GF RA   Vanini MM, Spisni A, Sforca ML, Pertinhez TA, Benedetti CE;
#=GF RL   Proteins. 2008;71:2051-2064.
#=GF RN   [2]
#=GF RM   19716795
#=GF RT   Insights into the bacterial transferrin receptor: the structure
#=GF RT   of transferrin-binding protein B from Actinobacillus
#=GF RT   pleuropneumoniae.
#=GF RA   Moraes TF, Yu RH, Strynadka NC, Schryvers AB;
#=GF RL   Mol Cell. 2009;35:523-533.
#=GF RN   [3]
#=GF RM   25800619
#=GF RT   Patterns of structural and sequence variation within isotype
#=GF RT   lineages of the Neisseria meningitidis transferrin receptor
#=GF RT   system.
#=GF RA   Adamiak P, Calmettes C, Moraes TF, Schryvers AB;
#=GF RL   Microbiologyopen. 2015;4:491-504.
#=GF RN   [4]
#=GF RM   23836816
#=GF RT   Conserved regions of gonococcal TbpB are critical for surface
#=GF RT   exposure and transferrin iron utilization.
#=GF RA   Ostberg KL, DeRocco AJ, Mistry SD, Dickinson MK, Cornelissen CN;
#=GF RL   Infect Immun. 2013;81:3442-3450.
#=GF DR   INTERPRO; IPR035313;
#=GF DR   SO; 0000417; polypeptide_domain;
#=GF CC   Bacterial lipoproteins represent a large group of specialized
#=GF CC   membrane proteins that perform a variety of functions including
#=GF CC   maintenance and stabilization of the cell envelope, protein
#=GF CC   targeting and transit to the outer membrane, membrane
#=GF CC   biogenesis, and cell adherence [1]. Pathogenic Gram-negative
#=GF CC   bacteria within the Neisseriaceae and Pasteurellaceae families
#=GF CC   rely on a specialized uptake system, characterized by an
#=GF CC   essential surface receptor complex that acquires iron from host
#=GF CC   transferrin (Tf) and transports the iron across the outer
#=GF CC   membrane. They have an iron uptake system composed of surface
#=GF CC   exposed lipoprotein, Tf-binding protein B (TbpB), and an
#=GF CC   integral outer-membrane protein, Tf-binding protein A (TbpA),
#=GF CC   that together function to extract iron from the host iron
#=GF CC   binding glycoprotein (Tf). TbpB is a bilobed (N and C lobe)
#=GF CC   lipid-anchored protein with each lobe consisting of an
#=GF CC   eight-stranded beta barrel flanked by a 'handle' domain made up
#=GF CC   of four (N lobe) or eight (C lobe) beta strands [2]. TbpB
#=GF CC   extends from the outer membrane surface by virtue of an
#=GF CC   N-terminal peptide region that is anchored to the outer membrane
#=GF CC   by fatty acyl chains on the N-terminal cysteine and is involved
#=GF CC   in the initial capture of iron-loaded Tf [3]. The 4-residue
#=GF CC   conserved LSAC motif found at the amino terminus of TbpB
#=GF CC   represents a prototypical lipobox, with the cysteine residue
#=GF CC   serving as the first amino acid in the mature protein which is
#=GF CC   subsequently modified by the addition of a diacyl glycerol. A
#=GF CC   second conserved motif of interest is located two amino acids
#=GF CC   downstream of the LSAC site. This region consists of four
#=GF CC   glycine residues in tandem. Deletion of the conserved
#=GF CC   polyglycine motif has significant negative effects on growth in
#=GF CC   certain conditions, while mutational analysis revealed that the
#=GF CC   LSAC motif constituting the lipobox of TbpB is necessary for
#=GF CC   lipidation and hence tethering of TbpB to the bacterial surface
#=GF CC   [4]. This domain family is found on the N-terminal region of
#=GF CC   TbpB proteins, which comprises the N lobe handle consisting of a
#=GF CC   four-stranded antiparallel beta sheets held together by a short
#=GF CC   surface-exposed alpha helix. Tf-binding activity primarily
#=GF CC   resides in the TbpB N lobe [2].
#=GF SQ   29
#=GS D5VBA9_MORCB/155-266      AC D5VBA9.1
#=GS A0A263HG41_9PAST/3-70     AC A0A263HG41.1
#=GS M4R4K2_BIBTR/44-172       AC M4R4K2.1
#=GS A0A1T0CTV0_9GAMM/46-176   AC A0A1T0CTV0.1
#=GS L2FAC4_9GAMM/72-167       AC L2FAC4.1
#=GS A3MYW7_ACTP2/48-184       AC A3MYW7.1
#=GS D5VBA9_MORCB/79-166       AC D5VBA9.1
#=GS A3N2L6_ACTP2/46-156       AC A3N2L6.1
#=GS A0A3P2A500_9NEIS/46-180   AC A0A3P2A500.1
#=GS A0A377I256_HAEPH/43-168   AC A0A377I256.1
#=GS A0A238HJX1_9NEIS/51-183   AC A0A238HJX1.1
#=GS Q9JYK4_NEIMB/42-189       AC Q9JYK4.1
#=GS A0A3S5F9J2_9NEIS/33-171   AC A0A3S5F9J2.1
#=GS TBPB_HAEIN/39-164         AC P44971.1
#=GS B0USI8_HISS2/46-201       AC B0USI8.1
#=GS A0A379CCD2_9PAST/53-196   AC A0A379CCD2.1
#=GS A0A448VQK9_9NEIS/42-192   AC A0A448VQK9.1
#=GS A0A4R2N7W3_9PAST/69-205   AC A0A4R2N7W3.1
#=GS A0A066UDY8_9GAMM/56-186   AC A0A066UDY8.1
#=GS Q5F6Q3_NEIG1/43-182       AC Q5F6Q3.1
#=GS F5S961_9NEIS/85-217       AC F5S961.1
#=GS A0A1B8PL73_MORNO/74-164   AC A0A1B8PL73.1
#=GS A0A066UEJ6_9GAMM/57-193   AC A0A066UEJ6.1
#=GS A0A420XHM2_9PAST/44-175   AC A0A420XHM2.1
#=GS A0A4V2SIV6_9PAST/40-182   AC A0A4V2SIV6.1
#=GS TBPB_NEIMB/43-193         AC Q9K0V0.1
#=GS TBPB_NEIMB/43-193         DR PDB; 3V8U B; 37-173;
#=GS TBPB_NEIMB/43-193         DR PDB; 3V8U A; 37-173;
#=GS A0A1B8PL73_MORNO/159-259  AC A0A1B8PL73.1
#=GS A0A1T0CEK1_9GAMM/81-269   AC A0A1T0CEK1.1
#=GS A0A3P2A7K4_9NEIS/66-203   AC A0A3P2A7K4.1
D5VBA9_MORCB/155-266                 gymakmdkadknaigdrik------.-----...--------------------------.--..-KDN............kDKSLS.EAEL.........................................aEKIK..E....DVRKS.PDFQK...--.......VLSSIKA..KTFHSNDG...................TTKATTRDLKYVDYGYYL....--VND.ANYLTVKTDKL..WNSGPVGGVFYNGSTTA...........................
A0A263HG41_9PAST/3-70                .........kfdknntelg------.-----...--------------------------.--..----.............-----.----..........................................----..-....-----.-----...--.......------D..EGIIYSHN...................EKQPNQRSLTYVRSGYVL....--GSN.KFDIRVKENTQ..YNAGLMGYVFYQGHQPS...........................
M4R4K2_BIBTR/44-172                  ..................s-LQDDN.SSARRtinNTEAAALLQPGFGFSAKIPRRNQRGE.A-..----.............FEKI-.-GDI..........................................TAIN..G....ELLSI.PHEQE...VR.......TYGEDED..DFIHSHDG...................KKLHRIRDLQFVRSGYLL....-HLGA.KLELNREPKVL..-RAGPTGYVYYLGTKPA...........................
A0A1T0CTV0_9GAMM/46-176              ................alt---DVAgTPRPS...DETLEALMEPSLGAAIKIPMRNTYPN.L-..-VEN.............EANLN.SDNI..........................................ITLA..D....GTLDI.PFVNE...LT.......STNGSSG..WVTHTHDG...................YGDTHKRKFDYVRSGYVQ....-DIEG.-RYIDRENKTW..-YRGANGYVYYRGINPA...........................
L2FAC4_9GAMM/72-167                  ...................TYQDVT.TEKRT...EENLSDLQKPALGMGMDLKVRNWHPS.N-..-KEE.............KVDLK.ETDL..........................................AKLD..G....NLTTQ.PYDKQ...IL.......DNLKNSK..S-IQDQAN...................------------------....-----.-----------..-----------------rertaitdntkrdnk............
A3MYW7_ACTP2/48-184                  ..................e-FGDDV.TEAKP...YEQLPEFLQPTLGSEIAIPRRIIGPK.A-..--EE.............RVELS.PDNI..........................................KRLG..G....SLDEI.PHAKE...IEdh..pavKKSGEKF..SLVYSHDN...................KSPQRTRNMDYVRSGFVF....ALGGF.STKKNEKQENV..YRSGKIGYVFYKGINPS...........................
D5VBA9_MORCB/79-166                  ...................KYQDVP.TDKNE...KAEVSDIQKPAMGYGMALSKINLYEQ.K-..----.............DISLD.ANNI..........................................ITLD..G....KKQVA.EGKK-...--.......-------..--------...................------------------....-----.-----------..-----------------splpfsldvenklldgymakmdkadkn
A3N2L6_ACTP2/46-156                  ..................n-YTDEE.TQKRK...KEELDKLMEPALGYVTKIPVNIPSVR.KT..EISE.............IDTV-.----..........................................--TD..E....SLSLV.PNEDK...LR.......TIANENY..GSVVTKSG...................SNT-----MNFVRSGYTI....-----.----DVDHYGL..---RDKGYVYYKGVHPS...........................
A0A3P2A500_9NEIS/46-180              ..................s-YTDEK.TTPRP..tEAQLSELMQPGLGFSAKIPRRSTGAI.RS..ELEE.............TATVK.PESI..........................................QRLN..G....NLNEP.PHQDE...IR.......KNAKFSG..TIIHSHDG...................LKPVRQRDLQYVRSGWVM....-DPLV.GLQISNGPPKR..VEYGQVGYVYYQGTQPS...........................
A0A377I256_HAEPH/43-168              ..................q-FKDEE.SQLTPv.pSKNLSALQSPTLGTAMKLKAHNLFDR.S-..--GK.............ENLFS.EEEY..........................................ETLD..F....NMIKA.RV---...--.......AASTADG..SIVDSNDG...................SSPYFTRLLNYVRSGVYY....---ND.SWRERDIPNKR..FLSGFYGYAFYFGENPS...........................
A0A238HJX1_9NEIS/51-183              ...................KYQDEA.TKKRK...TEDLNAMQEPELGFAMQIKRRNNHPR.N-..-KEE.............HVPLL.AEDT..........................................IKLN..Ge..tELDNF.KQREE...VQ.......KKNGEEI..HSFDFSDG..................kSRATGYTDFKYVRLGYFL....--KNA.VSEIDFSKNIY..-KNGPFGYVFYKGINPS...........................
Q9JYK4_NEIMB/42-189                  ...................KSKDVP.TPPPAg.sSVETTPVNRPAVGAAMRLPRRNIASY.KQ..DGTEipdk....hqaeeHLPLK.EKDI..........................................LFLD..G....TLKEQ.ADKLK...--.......KKINERY..SDVRVITS...................KKEEEKYQYQFVRAGYVFt..rAEGKD.NEKEKTSDGKEfvNRFSYDGFVYYSGERPS...........................
A0A3S5F9J2_9NEIS/33-171              ...................KFQDEK.TQPRT...EEEVSAFMQPGLGASIEIPRRGTGLA.GT..LSEE.............RVALT.PENI..........................................KRIG..G....-DLDI.PYVKD...--.......IKNHNKYtgSLIHSHDN..................sAGDHRKRDLEYVRSGWVAd..aNVGLD.FVNGDFGNGITrkVLFGSIGHVYYKGVQPA...........................
TBPB_HAEIN/39-164                    ...................RYQDDT.SNQRK...KSNLEKLSIPSLGGGMKLVAQNLRGR.EP..SLLN.............EDGYIiFSSL..........................................SKIE..D....DFKKE.NQSQE...--.......PTIGSID..EPSET---...................NSPQNHHGQQYVYSGLYY....----I.QSWRNFSNGKF..-YSGYYGYAYYFGKQTA...........................
B0USI8_HISS2/46-201                  ...................TYQDVP.TGQRQ...QEIVEEINSPALGYATEIPRRNISPM.PT..TGTKes.........naRVAIT.AQQVapls..................................mpfnSIKE..Df..iKRLIA.ENTKKn.aRGr....dvKYFDDTD..DVLFAHDG...................SNLAHKRDLQYVRVGYVLg..tRKIEL.VFSHDKKTRDQ..FPAGWVGYVFYQGTSPA...........................
A0A379CCD2_9PAST/53-196              ................kki---DDS.SDVKKs.nEQISDDFYKPKTGATADIPQAVTKGMwGN..TVDE.............EKEIV.IDEFf........................................aTDRN..DnpkhDELQA.DHDYQ...MG.......KPFGNAT..VIQHSNDG...................LNESKKRNYQFTNNGFEF....YETGL.AHHDKTTGNKA..IKGKLKGYIFYKGVNPS...........................
A0A448VQK9_9NEIS/42-192              .............vekvpe------.--PRQ..nPEEQAALKQPAAGFVMKGLRRNRAKH.DQ..NGNPlldasgnpteaeiYIPLN.PNDV..........................................EAVN..D....ADLTI.PKLKE...MQ.......NDPNNRG..AHHVYHTG...................KQQEGK--YQYIRFGHVTadypEVIEE.TVRDKEGDLKWytQREGWNVHTFYHGTSAA...........................
A0A4R2N7W3_9PAST/69-205              .......vddvraadkdgv------.-----...--------TPSLGVEVKIPRRSFGLG.GT..LDEVva.........piG-DLTsVYDV..........................................TQID..N...nDSKDA.PHYKE...VNe.....hNKSDPEV..RPIHSHDKl................dkDPRRNRSHFKYVKAGFIV....KSTSL.GFEGEGKNKRI..-EMGNNGYVYYKGVNPA...........................
A0A066UDY8_9GAMM/56-186              ................kad----DN.ANADT..kNRADSRLTMPVLGSVLAIPKRNQAYD.KNmnHLEE.............YVPLD.ENNI..........................................TTAH..T....DPVPA.LTEKL...--.......-KESYGD..KKIHQ---...................---SNDGRYNFVKAGWIFtglsPYETI.ETDKDTDQPKQ..-YTKGDGYLYYYGENP-t..........................
Q5F6Q3_NEIG1/43-182                  ...................KYQDVP.SKKPE...----ARKDQGGYGFAMRFKRRNWYPP.SN..PKEN.............EIRLS.EGDW..........................................EQTG..N....GNIKN.PSKQKn.iID.......ALSGNGE..APLQDSSQqg..............egiSKVTDYHDFKYVWSGFFY....KQIGN.TIKKDDSSSKIieARNGPDGYIFYKGTDPS...........................
F5S961_9NEIS/85-217                  ...................KYQDEA.TKKRK...TEDLNAMQEPELGFAMQIKRRNNHPS.N-..-KEE.............HVPLL.AEDI..........................................IKLN..Ge..tELDNF.KQREE...VQ.......KKNGEEI..HSFDFSDG..................kSRTTGYTDFKYVRLGYFL....--KNA.VSEIDFSKNIY..-KNGPFGYVFYKGINPS...........................
A0A1B8PL73_MORNO/74-164              ...................KYQDVP.TTPNN...KEQVSSIQEPAMGYAMELKLRNWMRG.Q-..--EE.............HAQIS.TDDV..........................................IKLE..G....GLEHK.PFDNS...IW.......QNIKNSK..EVQNAYNQ...................EKQHI-------------....-----.-----------..-----------------eeqir......................
A0A066UEJ6_9GAMM/57-193              ...................TYQDEQ.NTRRT...-LDV-DTQEPALGYAVEVPRRVFAMR.DT..PPEE............lTVNIM.PDKV..........................................KPTN..H....RLEDL.PKVFSetlVE.......HPKYVED..SLSYSHDV...................KSKDAIRDLQFVRSGYVI....AEKGI.EFDRTGGVFRQ..NPAGQYGHVFYQGVNPA...........................
A0A420XHM2_9PAST/44-175              ..................v-LKDVE.VKPRE..rVEQAEGIMKPAFGYEISTPIRNRHPN.GT..ENTG.............DITLS.SNNI..........................................KPLM..N....PDMDI.PHLDE...--.......IIQEAGY..AIYTHNPN..................gKKNQKREQYDHIKFGYVL....--NDE.YRKLDWSSNIY..-ETGPKGYIYFRGNEPS...........................
A0A4V2SIV6_9PAST/40-182              ........essqdskkiik------.-----...EGSTKKVEDLYLGYQVSLVRRNIATH.KD..GKLN.............EEKIK.VYHVrtgt.................................engsgLFLE..E....EIAQN.EQKLS...LQ.......NRQGRIG..KAFIL---...................TRDDRKQGFKHISLGFED...yNELGEpEEKGLKGKEDI..LRTGPTGTLYYQGENAS...........................
TBPB_NEIMB/43-193                    ...................KYQDVF.SEKPQ...----AQKDQGGYGFAMRLKRRNWYPQ.A-..-KED.............EVKLD.ESDW..........................................EATGlpD....EPKEL.PKRQKs.vIE.......KVETDSD..NNIYSSPYlkpsnhqngntgnginqpkNQAKDYENFKYVYSGWFY....KHAKR.EFNLKVEPKSA..-KNGDDGYIFYHGKEPS...........................
#=GR TBPB_NEIMB/43-193         SS    ...................XXXXXX.XXXXX...----XXXX---SEEEEE--B----TT.T-..-T--.............-B---.GGG-..........................................EE----S....STTS-.-HHHHH.HHH.......TS---S-..---EE-TT--------------------B-SSS---SSEEEEEEE....EEEEE.EEESSSTT-EE..-EEEEEEEEEEEESSB-...........................
A0A1B8PL73_MORNO/159-259             ................iee------.-----...----------------QIRRDNKQRP.DK..EKLD.............EVAL-.EAHI..........................................KKTL..D....DHLTN.LAKPI...--.......-YER---..NINYSHDK...................QNKARTRDLTYVRSGYIY....--HSG.YSNIDIPNKIA..-KTGFDGALFYKGEQTA...........................
A0A1T0CEK1_9GAMM/81-269              ...................KYQDVP.TTPNN...KEQVSSIQEPAMGFGMALSKINLYDR.QD..TPLDeknii..tldgkkQ----.---VakgeksplpfsldvenklldgymakmdqadknaigekikrdnKQIS..D....AELAK.KIKED...VRkspyfqqVLSSMQA..KTSHSNDQ...................TTKATTRDLQYVDYGYYL...vNDGNY.LTVRKTDDPKL..WNSGPVGGVFYNGTTTA...........................
A0A3P2A7K4_9NEIS/66-203              .................te--KDEV.TPPKV..sQQEAEGVMKPALGYSMPIPRRNVSRY.DA..DKKLlpdg....kqveeYLPLN.PQDL..........................................KALH..P....TDITAaPHAAD...--.......--LAKRQ..EGVRI-DT...................SN-PAQRGYEFVHAGYIF....-SDFH.RSKGEEAAGLL..-REGADGYIFYQGRNPA...........................
#=GC SS_cons                         ...................XXXXXX.XXXXX...----XXXX---SEEEEE--B----TT.T-..-T--.............-B---.GGG-..........................................EE----S....STTS-.-HHHHH.HHH.......TS---S-..---EE-TT--------------------B-SSS---SSEEEEEEE....EEEEE.EEESSSTT-EE..-EEEEEEEEEEEESSB-...........................
#=GC seq_cons                        ....................hpD...op.pp...ppphsth.pPuhGhuhpls+RNhtst.p....hpp.............chslp..psl..........................................ppls..s....shhph.spppc...h........thtsppt..phhpspss...................pptpppcshpaV+sGahh......ht....phchpstph...psG..GYlaYpGhpPu...........................
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