GenomeNet

Database: Pfam
Entry: bCoV_NSP1
LinkDB: bCoV_NSP1
Original site: bCoV_NSP1 
#=GF ID   bCoV_NSP1
#=GF AC   PF11501.10
#=GF DE   Betacoronavirus replicase NSP1
#=GF PI   Nsp1;
#=GF AU   Pollington J;0000-0002-8158-8998
#=GF AU   Finn RD;0000-0001-8626-2148
#=GF AU   Chuguransky S;0000-0002-0520-0736
#=GF SE   pdb_2gdt
#=GF GA   25.00 25.00;
#=GF TC   106.80 105.80;
#=GF NC   21.90 21.10;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -Z 57096847 -E 1000 --cpu 4 HMM pfamseq
#=GF TP   Family
#=GF RN   [1]
#=GF RM   17202208
#=GF RT   Novel beta-barrel fold in the nuclear magnetic resonance
#=GF RT   structure of the replicase nonstructural protein 1 from the
#=GF RT   severe acute respiratory syndrome coronavirus. 
#=GF RA   Almeida MS, Johnson MA, Herrmann T, Geralt M, Wuthrich K; 
#=GF RL   J Virol. 2007;81:3151-3161.
#=GF RN   [2]
#=GF RM   22174690
#=GF RT   SARS coronavirus nsp1 protein induces template-dependent
#=GF RT   endonucleolytic cleavage of mRNAs: viral mRNAs are resistant to
#=GF RT   nsp1-induced RNA cleavage.
#=GF RA   Huang C, Lokugamage KG, Rozovics JM, Narayanan K, Semler BL,
#=GF RA   Makino S;
#=GF RL   PLoS Pathog. 2011;7:e1002433.
#=GF RN   [3]
#=GF RM   19838190
#=GF RT   A two-pronged strategy to suppress host protein synthesis by
#=GF RT   SARS coronavirus Nsp1 protein.
#=GF RA   Kamitani W, Huang C, Narayanan K, Lokugamage KG, Makino S;
#=GF RL   Nat Struct Mol Biol. 2009;16:1134-1140.
#=GF RN   [4]
#=GF RM   25432065
#=GF RT   Coronavirus nonstructural protein 1: Common and distinct
#=GF RT   functions in the regulation of host and viral gene expression.
#=GF RA   Narayanan K, Ramirez SI, Lokugamage KG, Makino S;
#=GF RL   Virus Res. 2015;202:89-100.
#=GF RN   [5]
#=GF RM   23035226
#=GF RT   Severe acute respiratory syndrome coronavirus protein nsp1 is a
#=GF RT   novel eukaryotic  translation inhibitor that represses multiple
#=GF RT   steps of translation initiation.
#=GF RA   Lokugamage KG, Narayanan K, Huang C, Makino S;
#=GF RL   J Virol. 2012;86:13598-13608.
#=GF DR   INTERPRO; IPR021590;
#=GF DR   SO; 0100021; polypeptide_conserved_region;
#=GF CC   This entry represents the non structural protein NSP1 from
#=GF CC   Betacoronavirus NSP1 is the N-terminal cleavage product from the
#=GF CC   viral replicase that mediates RNA replication and processing
#=GF CC   [1]. Structurally, the protein consists of a mixed
#=GF CC   parallel/antiparallel 6-stranded beta barrel with an alpha helix
#=GF CC   covering one end of the barrel and another helix alongside the
#=GF CC   barrel [1]. NSP1 binds to the 40S ribosomal subunit and inhibits
#=GF CC   translation, and it also induces a template-dependent
#=GF CC   endonucleolytic cleavage of host mRNAs [2,3]. NSP1 also
#=GF CC   suppresses the host innate immune functions by inhibiting type I
#=GF CC   interferon expression and host antiviral signalling pathways
#=GF CC   [4,5].
#=GF SQ   39
#=GS A0A088DIE1_9BETC/7-140   AC A0A088DIE1.1
#=GS R1A_SARS/7-144           AC P0C6U8.1
#=GS R1AB_BCHK9/7-145         AC P0C6W5.1
#=GS T2B9U0_MERS/22-163       AC T2B9U0.1
#=GS B8Q8S4_SARS/7-144        AC B8Q8S4.1
#=GS E0ZN59_BCHK9/7-145       AC E0ZN59.1
#=GS B8Q8U7_SARS/7-144        AC B8Q8U7.1
#=GS Q6UZF5_SARS/7-144        AC Q6UZF5.1
#=GS A0A1B3Q5W8_9BETC/7-145   AC A0A1B3Q5W8.1
#=GS B8Q8U6_SARS/7-144        AC B8Q8U6.1
#=GS U5KNA9_9BETC/22-165      AC U5KNA9.1
#=GS R1AB_SARS2/7-144         AC P0DTD1.1
#=GS A0A0U1WHK4_SARS/7-144    AC A0A0U1WHK4.1
#=GS R1AB_BCHK4/22-165        AC P0C6W3.1
#=GS A0A023YA54_MERS/22-165   AC A0A023YA54.1
#=GS A3EXE7_BCHK5/22-165      AC A3EXE7.1
#=GS B8Q8S5_SARS/7-144        AC B8Q8S5.1
#=GS A3EXA2_BCHK4/22-165      AC A3EXA2.1
#=GS E0XIZ2_9BETC/7-144       AC E0XIZ2.1
#=GS R9QTB2_SARS/7-144        AC R9QTB2.1
#=GS A3EXC0_BCHK4/22-165      AC A3EXC0.1
#=GS F2YDB1_SARS/7-144        AC F2YDB1.1
#=GS T2B9I2_MERS/22-163       AC T2B9I2.1
#=GS R1AB_BCHK5/22-165        AC P0C6W4.1
#=GS A0A0U1UZ65_BCHK4/22-165  AC A0A0U1UZ65.1
#=GS R1A_BCHK9/7-145          AC P0C6T6.1
#=GS R1A_BCHK5/22-165         AC P0C6T5.1
#=GS A0A0K1YZY7_SARS/7-144    AC A0A0K1YZY7.1
#=GS R1A_BC279/7-144          AC P0C6F5.1
#=GS R1A_SARS2/7-144          AC P0DTC1.1
#=GS R1AB_SARS/7-144          AC P0C6X7.1
#=GS R1AB_SARS/7-144          DR PDB; 2HSX A; 1-116;
#=GS R1AB_SARS/7-144          DR PDB; 2GDT A; 1-116;
#=GS A0A0K1Z0N1_SARS/7-144    AC A0A0K1Z0N1.1
#=GS E0ZN51_BCHK9/7-145       AC E0ZN51.1
#=GS R1A_BCHK4/22-165         AC P0C6T4.1
#=GS R1AB_BC279/7-144         AC P0C6V9.1
#=GS A0A0U1WHG0_SARS/7-144    AC A0A0U1WHG0.1
#=GS A3EXI9_BCHK9/7-145       AC A3EXI9.1
#=GS U5LR11_9BETC/22-165      AC U5LR11.1
#=GS F2YDB2_SARS/7-144        AC F2YDB2.1
A0A088DIE1_9BETC/7-140              .e-LNQKTHFSVELPWIQIGE.-NPIKPWSVTPAEALNTVYAAIAE.-GKAAFIPPFK.GMHKYLPELCVFLVR--....NHGNPTRPFDLQELAVREGD....VNYGRSGETVGAIVPL.DGQEPYGTITIQLRR...RGKNAGGVYAWTNTRD.......
R1A_SARS/7-144                      .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalstNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
R1AB_BCHK9/7-145                    .p-PKLKSMVVTTLKWCDPFA.NPNVTGWDIPIEEALEYAKQQLRTpEPQLVFVPYYL.SHAPGISGDRVVITDSIwyatNFGWQ----PIRELAMDKDG....VRYGRGG-THGVLLPMqDPSFIMGDIDIQIRK...YGIGANSPPDVLPLWD.......
T2B9U0_MERS/22-163                  .s-EKHQDHVSLTVPLCGSGNlVEKLSPWFMDGENAYEVVKAMLLK.KEPLLYVPIRLaGHTRHLPGPRVYLVERL....IACEN--PFMVNQLAYSSSAngslVGTTLQGKPIGMFFPY.DIELVTGKQNILLRK...YGRGGYHYT-------pfhyerd
B8Q8S4_SARS/7-144                   .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalrtNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
E0ZN59_BCHK9/7-145                  .p-PKLKSMVTVTFKWIDPNI.MPSVTGWDVSMQEALELVKAELRKpTPKLVFVPNYL.CHTPLIGRDRIVITDSIyratVMGWI----PIRELAFDENN....IRCGRSG-TFGVLLPEqDPKYVMGEVDIDMRK...YGVGACSEKPVPLLWD.......
B8Q8U7_SARS/7-144                   .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalrtNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
Q6UZF5_SARS/7-144                   .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalstNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
A0A1B3Q5W8_9BETC/7-145              ..TNKLRSMVSITLDWIDPRN.VPRLTNWEMPLQEAAEYVKRELSKpNPDVVFVPNYL.LDSAQIPQNRVVITNSVwrcyEMGWK----PVREIAFDLDG....VRFGRGG-THGVLLPRqPPQYICGTVSIELRK...YGVGGGAEIFIPKLWD.......
B8Q8U6_SARS/7-144                   .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalrtNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
U5KNA9_9BETC/22-165                 ..NEKRHDHVALTVPCCGTEAkVTALSPWFMDGMLAYETVKEMLLK.GEQLLFAPSNLsGYIKFLPGPRVYLVERL....TGGTYSEPFIVNQLAFSDEQdgpmMGTTLQGKPIGFFFPF.DEELVTGTYTFKLRK...NGLGGQLFREVPWFEN.......
R1AB_SARS2/7-144                    gf--NEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEVLSEARQHLKD.-GTCGLVEVEK.GVLPQLEQPYVFIKRSDartaPHGHV-----MVELVAELEG....IQYGRSGETLGVLVPH.VGEIPVAYRKVLLRKngnKGAGGHSYGADLKSFD.......
A0A0U1WHK4_SARS/7-144               .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalstKHGHK-----VVELVAELDG....IQCGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSFGIDLKSYD.......
R1AB_BCHK4/22-165                   ..NEKRSDHVACTVPLCDTDDmACKLTPWFEDGETAFNQVSSILKE.KGKILFVPMHMqRAMKFLPGPRVYLVERL....TGGMLSKHFLVNQLAYKDQVgaamMRTTLNAKPLGMFFPY.DSSLETGEYTFLLRK...NGLGGQLFRERPWDR-k......
A0A023YA54_MERS/22-165              ..NEKRQDHVSQTVSFCGAGDlAMKLSPWFADGETACNTVKATLKN.KGKILFVPVKLaSFVKHLPGPRVYLVERL....TGGNYSDPFMVSQLAYSSSArdaiVGTTLQGKPVGYFFPY.DPALETGDYTFLLRK...DGFGGNYFRKPRPNPD.......
A3EXE7_BCHK5/22-165                 ..TEKRTDHVSLKASLCDAGDlVLKISPWFMDGESAYKHVSEQLSK.GSKLLFVPQTLkGFIRHLPGPRVYLVERL....TGGTYSDPFMVNQLAYQNAAgegvIGTTLQGKRVGMFFPF.DADLVTGEFQFLLRK...KGFGGNRFRDAPWDYN.......
B8Q8S5_SARS/7-144                   .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalrtNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
A3EXA2_BCHK4/22-165                 ..NEKRSDHVACTVPLCDTDDmACKLTPWFEDGETAFNQVSSILKE.KGKILFVPMHMqRAMKFLPGPRVYLVERL....TGGMLSKHFLVNQLAYKDQVgaamMRTTLNAKPLGMFFPY.DSSLETGEYTFLLRK...NGLGGQLFRERPWDR-k......
E0XIZ2_9BETC/7-144                  gf--NEKTHVQLSLPVLQVRD.-VLVRGFGDTVEEAVAEARQHLIE.-GTCGIVDLQK.GVLPQLEQPYIFLKRCDartaPHGHV-----MVELVAELDG....VQYGRSGESLGVLVPH.VGETPIGYRKVLVRKngnKGAGGHLYGADLRFYD.......
R9QTB2_SARS/7-144                   gv--SEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDaqstNHGHK-----VVELVAELNG....VQYGRSGTTLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
A3EXC0_BCHK4/22-165                 ..NEKRSDHVACTVPLCDTDDmACKLTPWFEDGETAFNQVSSILKE.KGKILFVPMHMqRAMKFLPGPRVYLVERL....TGGMLSKHFLVNQLAYKDHVgaalMRTTLNAKPLGMFFPY.DSSLETGEYTFLLRK...NGLGGQLFRERPWDR-k......
F2YDB1_SARS/7-144                   .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalstNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
T2B9I2_MERS/22-163                  .s-EKHQDHVSLTVPLCGSGNlVEKLSPWFMDGENAYEVVKAMLLK.KEPLLYVPIRLaGHTRHLPGPRVYLVERL....IACEN--PFMVNQLAYSSSAngslVGTTLQGKPIGMFFPY.DIELVTGKQNILLRK...YGRGGYHYT-------pfhyerd
R1AB_BCHK5/22-165                   ..TEKRTDHVSLKASLCDAGDlVLKISPWFMDGESAYKHVSEQLSK.GSKLLFVPQTLkGFIRHLPGPRVYLVERL....TGGTYSDPFMVNQLAYQNAAgegvIGTTLQGKRVGMFFPF.DADLVTGEFQFLLRK...KGFGGNRFRDAPWDYN.......
A0A0U1UZ65_BCHK4/22-165             ..NEKRSDHIACTVPLCDTEDmACKLTPWFEDGETAFNQVSSILKE.KGKILFVPMHMqMAMKFLPGPRVYLVERL....TGGMLSKHFLVNQLAYKDHVgaamMRTTLNAKPLGMFFPY.DSSLETGEYTFLLRK...NGLGGQLFRERPWNR-k......
R1A_BCHK9/7-145                     .p-PKLKSMVVTTLKWCDPFA.NPNVTGWDIPIEEALEYAKQQLRTpEPQLVFVPYYL.SHAPGISGDRVVITDSIwyatNFGWQ----PIRELAMDKDG....VRYGRGG-THGVLLPMqDPSFIMGDIDIQIRK...YGIGANSPPDVLPLWD.......
R1A_BCHK5/22-165                    ..TEKRTDHVSLKASLCDAGDlVLKISPWFMDGESAYKHVSEQLSK.GSKLLFVPQTLkGFIRHLPGPRVYLVERL....TGGTYSDPFMVNQLAYQNAAgegvIGTTLQGKRVGMFFPF.DADLVTGEFQFLLRK...KGFGGNRFRDAPWDYN.......
A0A0K1YZY7_SARS/7-144               .g-VNEKTHVQLSLPVLQVGD.-VLVRGFGDTVEEALTEAREHLKN.-GTCGLVELEK.GVLRQLEEPYVFIKRSEalstTHGHK-----VVELVAEMNG....IQFGRSGTTLGVLVPH.VGETPIAYRNVLLRKsgnKGAGGHSYGIDLKSYD.......
R1A_BC279/7-144                     gv--SEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALAEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSD....AQGTN-HGYKVVELVAELDG....IQYGRSGTTLGVLVPH.VGETPVAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
R1A_SARS2/7-144                     gf--NEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEVLSEARQHLKD.-GTCGLVEVEK.GVLPQLEQPYVFIKRSDartaPHGHV-----MVELVAELEG....IQYGRSGETLGVLVPH.VGEIPVAYRKVLLRKngnKGAGGHSYGADLKSFD.......
R1AB_SARS/7-144                     .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalstNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
#=GR R1AB_SARS/7-144          SS    .X-XXXX--EEEEEEE--TTT.--S---S-SSHHHHHHHHHHHHHH.-T-EEEE---T.TSGGGS-S-EEEEE--SS-----SS--------EEEEESS-....TTTTSSSS--EEEEE-.-S---SEEEEEEE----XXXXXXXXXXXXXXXXX.......
A0A0K1Z0N1_SARS/7-144               .g-VNEKTHVQLSLPVLQVGD.-VLVRGFGDTVEEALTEAREHLKN.-GTCGLVELEK.GVLRQLEEPYVFIKRSEalstTHGHK-----VVELVAEMNG....IQFGRSGTTLGVLVPH.VGETPIAYRNVLLRKsgnKGAGGHSYGIDLKSYD.......
E0ZN51_BCHK9/7-145                  .p-PKLRSMVTITLKWIDPNI.MPSVTGWDMPMCEALEIVKDELRKpEPDLVFVPKYL.CHTPLIGRNRVVITDAT....YRTTVLGRIPIRELAYDESE....TRYGRSG-TYGVLLPMqDSKYIMGSIDIDMRK...YGAGAGSDRPEPMLWD.......
R1A_BCHK4/22-165                    ..NEKRSDHVACTVPLCDTDDmACKLTPWFEDGETAFNQVSSILKE.KGKILFVPMHMqRAMKFLPGPRVYLVERL....TGGMLSKHFLVNQLAYKDQVgaamMRTTLNAKPLGMFFPY.DSSLETGEYTFLLRK...NGLGGQLFRERPWDR-k......
R1AB_BC279/7-144                    gv--SEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALAEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSD....AQGTN-HGYKVVELVAELDG....IQYGRSGTTLGVLVPH.VGETPVAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
A0A0U1WHG0_SARS/7-144               .g-VNEKTHVQLSLPVLQVSD.-VLVRGFGDTVEEALAEAREHLKN.-GTCGLVELEK.GVLRQLEEPYVFIKRSEalstTHGHK-----VVELVAEMNG....IQFGRSGTTLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
A3EXI9_BCHK9/7-145                  .p-PKLKSMVTVTFKWIDPNI.MPSVTGWDMPMQEALEVVKNELRKpEPQLVFVPKYL.CHTPLIGEDRIVVTDSTfratNMGWV----PIRELAFDENR....IRFGRSG-TFGVLLPMqDAKYIMGHIDIDMRK...YGVGAGGDKDEPLLWD.......
U5LR11_9BETC/22-165                 ..NEKRHDHVALTVPCCGTEAkVTALSPWFMDGMLAYETVKEMLLK.GEQLLFAPSNLsGYIKFLPGPRVYLVERL....TGGTYSEPFIVNQLAFSDEQdgpmMGTTLQGKPIGFFFPF.DEELVTGTYTFKLRK...NGLGGQLFREVPWFEN.......
F2YDB2_SARS/7-144                   .g-VNEKTHVQLSLPVLQVRD.-VLVRGFGDSVEEALSEAREHLKN.-GTCGLVELEK.GVLPQLEQPYVFIKRSDalstNHGHK-----VVELVAEMDG....IQYGRSGITLGVLVPH.VGETPIAYRNVLLRKngnKGAGGHSYGIDLKSYD.......
#=GC SS_cons                        .X-XXXX--EEEEEEE--TTT.--S---S-SSHHHHHHHHHHHHHH.-T-EEEE---T.TSGGGS-S-EEEEE--SS-----SS--------EEEEESS-....TTTTSSSS--EEEEE-.-S---SEEEEEEE----XXXXXXXXXXXXXXXXX.......
#=GC seq_cons                       ....pcKoHVpLoLPllpscD..shVpGas-ssEEALsps+ppLKp..GphshV.hch.Gsl.pLtpPhValpcu.....stGph.....VsELsh-.su....lpaGRuGhTLGVLlPa.su-hshuhhslLLRK...cGhGGpuat.c.hsaD.......
//
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