LOCUS NC_001133 230218 bp DNA linear CON 23-JAN-2026
DEFINITION Saccharomyces cerevisiae S288C chromosome I, complete sequence.
ACCESSION NC_001133
VERSION NC_001133.9
DBLINK BioProject: PRJNA128
Assembly: GCF_000146045.2
KEYWORDS RefSeq.
SOURCE Saccharomyces cerevisiae S288C
ORGANISM Saccharomyces cerevisiae S288C
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina;
Saccharomycetes; Saccharomycetales; Saccharomycetaceae;
Saccharomyces.
REFERENCE 1 (bases 1 to 230218)
AUTHORS Engel,S.R., Wong,E.D., Nash,R.S., Aleksander,S., Alexander,M.,
Douglass,E., Karra,K., Miyasato,S.R., Simison,M., Skrzypek,M.S.,
Weng,S. and Cherry,J.M.
TITLE New data and collaborations at the Saccharomyces Genome Database:
updated reference genome, alleles, and the Alliance of Genome
Resources
JOURNAL Genetics 220 (4) (2022)
PUBMED 34897464
REFERENCE 2 (bases 1 to 230218)
AUTHORS Goffeau,A., Barrell,B.G., Bussey,H., Davis,R.W., Dujon,B.,
Feldmann,H., Galibert,F., Hoheisel,J.D., Jacq,C., Johnston,M.,
Louis,E.J., Mewes,H.W., Murakami,Y., Philippsen,P., Tettelin,H. and
Oliver,S.G.
TITLE Life with 6000 genes
JOURNAL Science 274 (5287), 546 (1996)
PUBMED 8849441
REFERENCE 3 (bases 1 to 230218)
AUTHORS Bussey,H., Kaback,D.B., Zhong,W., Vo,D.T., Clark,M.W., Fortin,N.,
Hall,J., Ouellette,B.F., Keng,T., Barton,A.B. et al.
TITLE The nucleotide sequence of chromosome I from Saccharomyces
cerevisiae
JOURNAL Proc. Natl. Acad. Sci. U.S.A. 92 (9), 3809-3813 (1995)
PUBMED 7731988
REFERENCE 4 (bases 1 to 230218)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (23-JAN-2026) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 5 (bases 1 to 230218)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (31-MAR-2011) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
REMARK Sequence update by submitter
REFERENCE 6 (bases 1 to 230218)
CONSRTM Saccharomyces Genome Database
TITLE Direct Submission
JOURNAL Submitted (11-DEC-2009) Department of Genetics, Stanford
University, Stanford, CA 94305-5120, USA
COMMENT REVIEWED REFSEQ: This record has been curated by SGD. The reference
sequence is identical to BK006935.
On Apr 26, 2011 this sequence version replaced NC_001133.8.
##Genome-Annotation-Data-START##
Annotation Provider :: SGD
Annotation Status :: Full Annotation
Annotation Version :: R64-5-1
URL :: http://www.yeastgenome.org/
##Genome-Annotation-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..230218
/organism="Saccharomyces cerevisiae S288C"
/mol_type="genomic DNA"
/strain="S288C"
/db_xref="taxon:559292"
/chromosome="I"
telomere complement(1..801)
/note="TEL01L; Telomeric region on the left arm of
Chromosome I; composed of an X element core sequence, X
element combinatorial repeats, and a short terminal
stretch of telomeric repeats"
/db_xref="SGD:S000028862"
rep_origin 707..776
/note="ARS102; Autonomously Replicating Sequence"
/db_xref="SGD:S000121252"
gene complement(<1807..>2169)
/gene="PAU8"
/locus_tag="YAL068C"
/db_xref="GeneID:851229"
mRNA complement(<1807..>2169)
/gene="PAU8"
/locus_tag="YAL068C"
/product="seripauperin PAU8"
/transcript_id="NM_001180043.1"
/db_xref="GeneID:851229"
CDS complement(1807..2169)
/gene="PAU8"
/locus_tag="YAL068C"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:12586695]"
/note="hypothetical protein; member of the seripauperin
multigene family encoded mainly in subtelomeric regions"
/codon_start=1
/product="seripauperin PAU8"
/protein_id="NP_009332.1"
/db_xref="GeneID:851229"
/db_xref="SGD:S000002142"
/translation="MVKLTSIAAGVAAIAATASATTTLAQSDERVNLVELGVYVSDIR
AHLAQYYMFQAAHPTETYPVEVAEAVFNYGDFTTMLTGIAPDQVTRMITGVPWYSSRL
KPAISSALSKDGIYTIAN"
gene <2480..>2707
/locus_tag="YAL067W-A"
/db_xref="GeneID:1466426"
mRNA <2480..>2707
/locus_tag="YAL067W-A"
/product="uncharacterized protein"
/transcript_id="NM_001184582.1"
/db_xref="GeneID:1466426"
CDS 2480..2707
/locus_tag="YAL067W-A"
/note="hypothetical protein; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878038.1"
/db_xref="GeneID:1466426"
/db_xref="SGD:S000028593"
/translation="MPIIGVPRCLIKPFSVPVTFPFSVKKNIRILDLDPRTEAYCLSL
NSVCFKRLPRRKYFHLLNSYNIKRVLGVVYC"
gene complement(<7235..>9016)
/gene="SEO1"
/locus_tag="YAL067C"
/db_xref="GeneID:851230"
mRNA complement(<7235..>9016)
/gene="SEO1"
/locus_tag="YAL067C"
/product="putative permease SEO1"
/transcript_id="NM_001178208.1"
/db_xref="GeneID:851230"
CDS complement(7235..9016)
/gene="SEO1"
/locus_tag="YAL067C"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:40288644]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:genetic interaction:GO:0042938
dipeptide transport [PMID:40288644]"
/experiment="EXISTENCE:genetic interaction:GO:0071916
dipeptide transmembrane transporter activity
[PMID:40288644]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:8893857]"
/note="High affinity dipeptide transmembrane transporter;
involved in the transport of the gamma-Glu-met dipeptide
across the plasma membrane; member of the allantoate
transporter subfamily of the major facilitator
superfamily; transcriptionally upregulated in cells grown
on gamma-Glu-met; mutation confers resistance to ethionine
sulfoxide"
/codon_start=1
/product="putative permease SEO1"
/protein_id="NP_009333.1"
/db_xref="GeneID:851230"
/db_xref="SGD:S000000062"
/translation="MYSIVKEIIVDPYKRLKWGFIPVKRQVEDLPDDLNSTEIVTISN
SIQSHETAENFITTTSEKDQLHFETSSYSEHKDNVNVTRSYEYRDEADRPWWRFFDEQ
EYRINEKERSHNKWYSWFKQGTSFKEKKLLIKLDVLLAFYSCIAYWVKYLDTVNINNA
YVSGMKEDLGFQGNDLVHTQVMYTVGNIIFQLPFLIYLNKLPLNYVLPSLDLCWSLLT
VGAAYVNSVPHLKAIRFFIGAFEAPSYLAYQYLFGSFYKHDEMVRRSAFYYLGQYIGI
LSAGGIQSAVYSSLNGVNGLEGWRWNFIIDAIVSVVVGLIGFYSLPGDPYNCYSIFLT
DDEIRLARKRLKENQTGKSDFETKVFDIKLWKTIFSDWKIYILTLWNIFCWNDSNVSS
GAYLLWLKSLKRYSIPKLNQLSMITPGLGMVYLMLTGIIADKLHSRWFAIIFTQVFNI
IGNSILAAWDVAEGAKWFAFMLQCFGWAMAPVLYSWQNDICRRDAQTRAITLVTMNIM
AQSSTAWISVLVWKTEEAPRYLKGFTFTACSAFCLSIWTFVVLYFYKRDERNNAKKNG
IVLYNSKHGVEKPTSKDVETLSVSDEK"
rep_origin 7997..8547
/note="ARS103; Autonomously Replicating Sequence;
replication origin of very weak function"
/db_xref="SGD:S000121253"
gene complement(<11565..>11951)
/locus_tag="YAL065C"
/db_xref="GeneID:851232"
mRNA complement(<11565..>11951)
/locus_tag="YAL065C"
/product="uncharacterized protein"
/transcript_id="NM_001179897.1"
/db_xref="GeneID:851232"
CDS complement(11565..11951)
/locus_tag="YAL065C"
/note="hypothetical protein; shows sequence similarity to
FLO1 and other flocculins"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_009335.1"
/db_xref="GeneID:851232"
/db_xref="SGD:S000001817"
/translation="MNSATSETTTNTGAAETTTSTGAAETKTVVTSSISRFNHAETQT
ASATDVIGHSSSVVSVSETGNTKSLITSGLSTMSQQPRSTPASSIIGSSTASLEISTY
VGIANGLLTNNGISVFISTVLLAIVW"
gene <12046..>12426
/locus_tag="YAL064W-B"
/db_xref="GeneID:851233"
mRNA <12046..>12426
/locus_tag="YAL064W-B"
/product="uncharacterized protein"
/transcript_id="NM_001180042.1"
/db_xref="GeneID:851233"
CDS 12046..12426
/locus_tag="YAL064W-B"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/note="Fungal-specific hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_009336.1"
/db_xref="GeneID:851233"
/db_xref="SGD:S000002141"
/translation="MAGEAVSEHTPDSQEVTVTSVVCCLDSVVEIGHHVVYSVVTPLI
VAVLIDTMAGEAVLEHTSDSQEEIVTTVVCSVVPLVCFVVSVVCFVISVVEIGHHVVY
SVVAPLTVTVAVETIAEEMDSVHT"
gene complement(<13363..>13743)
/gene="TDA8"
/locus_tag="YAL064C-A"
/gene_synonym="YAL065C-A"
/db_xref="GeneID:851234"
mRNA complement(<13363..>13743)
/gene="TDA8"
/locus_tag="YAL064C-A"
/gene_synonym="YAL065C-A"
/product="Tda8p"
/transcript_id="NM_001180041.1"
/db_xref="GeneID:851234"
CDS complement(13363..13743)
/gene="TDA8"
/locus_tag="YAL064C-A"
/gene_synonym="YAL065C-A"
/note="hypothetical protein; null mutant is sensitive to
expression of the top1-T722A allele; not an essential
gene"
/codon_start=1
/product="Tda8p"
/protein_id="NP_058136.1"
/db_xref="GeneID:851234"
/db_xref="SGD:S000002140"
/translation="MTGYFLPPQTSSYTFRFAKVDDSAILSVGGDVAFGCCAQEQPPI
TSTNFTINGIKPWQGRLPDNIAGTVYMYAGFYCPMKIVYSNAVSWHTLPVSVELPDVT
TVSDDFAGHVYSFDDDLTAQLYYP"
gene <21566..>21850
/locus_tag="YAL064W"
/db_xref="GeneID:851235"
mRNA <21566..>21850
/locus_tag="YAL064W"
/product="uncharacterized protein"
/transcript_id="NM_001178206.2"
/db_xref="GeneID:851235"
CDS 21566..21850
/locus_tag="YAL064W"
/note="hypothetical protein; may interact with ribosomes,
based on co-purification experiments"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_009337.2"
/db_xref="GeneID:851235"
/db_xref="SGD:S000000060"
/translation="MNPFASLEGQDNISSVFFLHMQQFESQVKDRFRFPIFRLERKTF
GNSCYQVETLKVKCRPRHAKSCNLLTLLFKSRTQSVLVPNFGFLILNSEP"
repeat_region 22230..22552
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006787"
gene complement(<22395..>22685)
/locus_tag="YAL063C-A"
/db_xref="GeneID:1466427"
mRNA complement(<22395..>22685)
/locus_tag="YAL063C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184642.1"
/db_xref="GeneID:1466427"
CDS complement(22395..22685)
/locus_tag="YAL063C-A"
/note="hypothetical protein; identified by expression
profiling and mass spectrometry"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878039.1"
/db_xref="GeneID:1466427"
/db_xref="SGD:S000028813"
/translation="MCPRTVLLIININHWFYDKNIVRIILTFRLDSGHISDICFINKN
LANALITADISLLKRHDIRCTKYIITYYQRYRNKEKGKFISLCKNTIISSSV"
gene complement(<24000..>27968)
/gene="FLO9"
/locus_tag="YAL063C"
/db_xref="GeneID:851236"
mRNA complement(<24000..>27968)
/gene="FLO9"
/locus_tag="YAL063C"
/product="flocculin FLO9"
/transcript_id="NM_001178205.1"
/db_xref="GeneID:851236"
CDS complement(24000..27968)
/gene="FLO9"
/locus_tag="YAL063C"
/experiment="EXISTENCE:mutant phenotype:GO:0000128
flocculation [PMID:19420680|PMID:19087208]"
/experiment="EXISTENCE:mutant phenotype:GO:0031589
cell-substrate adhesion [PMID:19087208]"
/note="Lectin-like protein with similarity to Flo1p;
thought to be expressed and involved in flocculation"
/codon_start=1
/product="flocculin FLO9"
/protein_id="NP_009338.1"
/db_xref="GeneID:851236"
/db_xref="SGD:S000000059"
/translation="MSLAHYCLLLAIVTLLGLTNVVSATTAACLPANSRKNGMNVNFY
QYSLRDSSTYSNAAYMAYGYASKTKLGSVGGQTDISIDYNIPCVSSSGTFPCPQEDLY
GNWGCKGIGACSNNPIIAYWSTDLFGFYTTPTNVTLEMTGYFLPPQTGSYTFKFATVD
DSAILSVGGSIAFECCAQEQPPITSTNFTINGIKPWNGSPPDNITGTVYMYAGFYYPM
KIVYSNAVAWGTLPISVTLPDGTTVSDDFEGYVYTFDNNLSQPNCTIPDPSNYTVSTT
ITTTEPWTGTFTSTSTEMTTVTGTNGVPTDETVIVIRTPTTASTIITTTEPWNSTFTS
TSTELTTVTGTNGVRTDETIIVIRTPTTATTAITTTEPWNSTFTSTSTELTTVTGTNG
LPTDETIIVIRTPTTATTAMTTTQPWNDTFTSTSTELTTVTGTNGLPTDETIIVIRTP
TTATTAMTTTQPWNDTFTSTSTELTTVTGTNGLPTDETIIVIRTPTTATTAMTTTQPW
NDTFTSTSTEITTVTGTNGLPTDETIIVIRTPTTATTAMTTTQPWNDTFTSTSTEMTT
VTGTNGLPTDETIIVIRTPTTATTAITTTEPWNSTFTSTSTEMTTVTGTNGLPTDETI
IVIRTPTTATTAITTTQPWNDTFTSTSTEMTTVTGTNGLPTDETIIVIRTPTTATTAM
TTTQPWNDTFTSTSTEITTVTGTNGLPTDETIIVIRTPTTATTAMTTTQPWNDTFTST
STEMTTVTGTNGVPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEMTTVTGTNGQ
PTDETVIVIRTPTSEGLVTTTTEPWTGTFTSTSTEMTTITGTNGQPTDETVIIVKTPT
TAISSSLSSSSGQITSFITSARPIITPFYPSNGTSVISSSVISSSDTSSLVISSSVTS
SLVTSSPVISSSFISSPVISSTTTSASILSESSKSSVIPTSSSTSGSSESETGSASSA
SSSSSISSESPKSTYSSSSLPPVTSATTSQEITSSLPPVTTTKTSEQTTLVTVTSCES
HVCTESISSAIVSTATVTVSGATTEYTTWCPISTTEITKQTTETTKQTKGTTEQTTET
TKQTTVVTISSCESDVCSKTASPAIVSTSTATINGVTTEYTTWCPISTTESKQQTTLV
TVTSCGSGVCSETTSPAIVSTATATVNDVVTVYSTWRPQTTNEQSVSSKMNSATSETT
TNTGAAETTTSTGAAETKTVVTSSISRFNHAETQTASATDVIGHSSSVVSVSETGNTK
SLTSSGLSTMSQQPRSTPASSMVGSSTASLEISTYAGSANSLLAGSGLSVFIASLLLA
II"
rep_origin 30946..31183
/note="ARS104; Autonomously Replicating Sequence"
/db_xref="SGD:S000118317"
gene <31567..>32940
/gene="GDH3"
/locus_tag="YAL062W"
/gene_synonym="FUN51"
/db_xref="GeneID:851237"
mRNA <31567..>32940
/gene="GDH3"
/locus_tag="YAL062W"
/gene_synonym="FUN51"
/product="glutamate dehydrogenase (NADP(+)) GDH3"
/transcript_id="NM_001178204.1"
/db_xref="GeneID:851237"
CDS 31567..32940
/gene="GDH3"
/locus_tag="YAL062W"
/gene_synonym="FUN51"
/EC_number="1.4.1.4"
/experiment="EXISTENCE:direct assay:GO:0004354 glutamate
dehydrogenase (NADP+) activity [PMID:11562373]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:4126]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:mutant phenotype:GO:0004354
glutamate dehydrogenase (NADP+) activity [PMID:9287019]"
/experiment="EXISTENCE:mutant phenotype:GO:0006537
glutamate biosynthetic process [PMID:9287019]"
/note="NADP(+)-dependent glutamate dehydrogenase;
synthesizes glutamate from ammonia and
alpha-ketoglutarate; rate of alpha-ketoglutarate
utilization differs from Gdh1p; expression regulated by
nitrogen and carbon sources; GDH3 has a paralog, GDH1,
that arose from the whole genome duplication"
/codon_start=1
/product="glutamate dehydrogenase (NADP(+)) GDH3"
/protein_id="NP_009339.1"
/db_xref="GeneID:851237"
/db_xref="SGD:S000000058"
/translation="MTSEPEFQQAYDEIVSSVEDSKIFEKFPQYKKVLPIVSVPERII
QFRVTWENDNGEQEVAQGYRVQFNSAKGPYKGGLRFHPSVNLSILKFLGFEQIFKNAL
TGLDMGGGKGGLCVDLKGKSDNEIRRICYAFMRELSRHIGKDTDVPAGDIGVGGREIG
YLFGAYRSYKNSWEGVLTGKGLNWGGSLIRPEATGFGLVYYTQAMIDYATNGKESFEG
KRVTISGSGNVAQYAALKVIELGGIVVSLSDSKGCIISETGITSEQIHDIASAKIRFK
SLEEIVDEYSTFSESKMKYVAGARPWTHVSNVDIALPCATQNEVSGDEAKALVASGVK
FVAEGANMGSTPEAISVFETARSTATNAKDAVWFGPPKAANLGGVAVSGLEMAQNSQK
VTWTAERVDQELKKIMINCFNDCIQAAQEYSTEKNTNTLPSLVKGANIASFVMVADAM
LDQGDVF"
gene <33448..>34701
/gene="BDH2"
/locus_tag="YAL061W"
/db_xref="GeneID:851238"
mRNA <33448..>34701
/gene="BDH2"
/locus_tag="YAL061W"
/product="putative dehydrogenase BDH2"
/transcript_id="NM_001178203.1"
/db_xref="GeneID:851238"
CDS 33448..34701
/gene="BDH2"
/locus_tag="YAL061W"
/EC_number="1.1.1.303"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Putative medium-chain alcohol dehydrogenase with
similarity to BDH1; transcription induced by
constitutively active PDR1 and PDR3"
/codon_start=1
/product="putative dehydrogenase BDH2"
/protein_id="NP_009340.1"
/db_xref="GeneID:851238"
/db_xref="SGD:S000000057"
/translation="MRALAYFGKGNIRFTNHLKEPHIVAPDELVIDIEWCGICGTDLH
EYTDGPIFFPEDGHTHEISHNPLPQAMGHEMAGTVLEVGPGVKNLKVGDKVVVEPTGT
CRDRYRWPLSPNVDKEWCAACKKGYYNICSYLGLCGAGVQSGGFAERVVMNESHCYKV
PDFVPLDVAALIQPLAVCWHAIRVCEFKAGSTALIIGAGPIGLGTILALNAAGCKDIV
VSEPAKVRRELAEKMGARVYDPTAHAAKESIDYLRSIADGGDGFDYTFDCSGLEVTLN
AAIQCLTFRGTAVNLAMWGHHKIQFSPMDITLHERKYTGSMCYTHHDFEAVIEALEEG
RIDIDRARHMITGRVNIEDGLDGAIMKLINEKESTIKIILTPNNHGELNREADNEKKE
ISELSSRKDQERLRESINEAKLRHT"
gene <35155..>36303
/gene="BDH1"
/locus_tag="YAL060W"
/db_xref="GeneID:851239"
mRNA <35155..>36303
/gene="BDH1"
/locus_tag="YAL060W"
/product="(R,R)-butanediol dehydrogenase"
/transcript_id="NM_001178202.2"
/db_xref="GeneID:851239"
CDS 35155..36303
/gene="BDH1"
/locus_tag="YAL060W"
/EC_number="1.1.1.4"
/experiment="EXISTENCE:direct assay:GO:0000721
(R,R)-butanediol dehydrogenase activity [PMID:10938079]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:14562095]"
/experiment="EXISTENCE:mutant phenotype:GO:0006066 alcohol
metabolic process [PMID:10938079]"
/experiment="EXISTENCE:mutant phenotype:GO:0034079
butanediol biosynthetic process [PMID:19966022]"
/note="NAD-dependent (R,R)-butanediol dehydrogenase;
catalyzes oxidation of (R,R)-2,3-butanediol to
(3R)-acetoin, oxidation of meso-butanediol to
(3S)-acetoin, and reduction of acetoin; enhances use of
2,3-butanediol as an aerobic carbon source"
/codon_start=1
/product="(R,R)-butanediol dehydrogenase"
/protein_id="NP_009341.2"
/db_xref="GeneID:851239"
/db_xref="SGD:S000000056"
/translation="MRALAYFKKGDIHFTNDIPRPEIQTDDEVIIDVSWCGICGSDLH
EYLDGPIFMPKDGECHKLSNAALPLAMGHEMSGIVSKVGPKVTKVKVGDHVVVDAASS
CADLHCWPHSKFYNSKPCDACQRGSENLCTHAGFVGLGVISGGFAEQVVVSQHHIIPV
PKEIPLDVAALVEPLSVTWHAVKISGFKKGSSALVLGAGPIGLCTILVLKGMGASKIV
VSEIAERRIEMAKKLGVEVFNPSKHGHKSIEILRGLTKSHDGFDYSYDCSGIQVTFET
SLKALTFKGTATNIAVWGPKPVPFQPMDVTLQEKVMTGSIGYVVEDFEEVVRAIHNGD
IAMEDCKQLITGKQRIEDGWEKGFQELMDHKESNVKILLTPNNHGEMK"
gene <36509..>37147
/gene="ECM1"
/locus_tag="YAL059W"
/db_xref="GeneID:851240"
mRNA <36509..>37147
/gene="ECM1"
/locus_tag="YAL059W"
/product="Ecm1p"
/transcript_id="NM_001178201.2"
/db_xref="GeneID:851240"
CDS 36509..37147
/gene="ECM1"
/locus_tag="YAL059W"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11583615|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:11583615]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:20348449]"
/experiment="EXISTENCE:direct assay:GO:1990275 preribosome
binding [PMID:35139378]"
/experiment="EXISTENCE:genetic interaction:GO:0000055
ribosomal large subunit export from nucleus
[PMID:11583615|PMID:20348449]"
/note="Pre-ribosomal factor involved in 60S ribosomal
protein subunit export; associates with the pre-60S
particle; shuttles between the nucleus and cytoplasm"
/codon_start=1
/product="Ecm1p"
/protein_id="NP_009342.2"
/db_xref="GeneID:851240"
/db_xref="SGD:S000000055"
/translation="MWEQRRQKVVFSLTILVRYRLKQSMAKKISKNSRAARQSDALEP
EVKDLSELPRAEKTDLTNILIRTAAKNEALLEAKISKKANKSKRGKKLNKKALEDKLA
NSISSMDRDRLVKALNFTNRLDGKIAKSISRAKYIQNTRKAGWDSTNETIKKELAFLN
GGLSVQAKSASEGNAEKEDEEIPEVFDSLAEDNTVQKTPTNRFGVLPDDVEE"
gene <37464..>38972
/gene="CNE1"
/locus_tag="YAL058W"
/gene_synonym="FUN48"
/db_xref="GeneID:851241"
mRNA <37464..>38972
/gene="CNE1"
/locus_tag="YAL058W"
/gene_synonym="FUN48"
/product="calnexin"
/transcript_id="NM_001178200.1"
/db_xref="GeneID:851241"
CDS 37464..38972
/gene="CNE1"
/locus_tag="YAL058W"
/gene_synonym="FUN48"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:7814381]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:16002399]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:15173200]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:7814381]"
/experiment="EXISTENCE:mutant phenotype:GO:0051082
unfolded protein binding [PMID:15173200]"
/experiment="EXISTENCE:physical interaction:GO:0005515
protein binding [PMID:16002399]"
/note="Calnexin; integral membrane ER chaperone involved
in folding and quality control of glycoproteins; chaperone
activity is inhibited by Mpd1p, with which Cne1p
interacts; 24% identical to mammalian calnexin; Ca+
binding not yet shown in yeast"
/codon_start=1
/product="calnexin"
/protein_id="NP_009343.1"
/db_xref="GeneID:851241"
/db_xref="SGD:S000000054"
/translation="MKFSAYLWWLFLNLALVKGTSLLSNVTLAEDSFWEHFQAYTNTK
HLNQEWITSEAVNNEGSKIYGAQWRLSQGRLQGSAWDKGIAVRTGNAAAMIGHLLETP
INVSETDTLVVQYEIKLDNSLTCGGAFIKLMSGFMNVEALKHYAPDTEGVELVFGPDY
CAPEINGVQFAINKVDKITHESKLRYLQEMPLSKLTDTSQSHLYTLIIDESAQSFQIL
IDGKTVMVREHIEDKKKVNFEPPITPPLMIPDVSVAKPHDWDDRIRIPDPEAVKLSDR
DERDPLMIPHPDGTEPPEWNSSIPEYILDPNAQKPSWWKELEHGEWIPPMIKNPLCTA
ERGCGQQIPGLINNAKYKGPGELNEIINPNYMGEWHPPEIENPLYYEEQHPLRIENVI
SGVILEFWSGSPNMLISNIYVGKNVTEAQIIGNKTWLMRDRAFRGSDGPTERKFMNSR
LGNLQTTFHNERESPNPFDRIIDRILEQPLKFVLTAAVVLLTTSVLCCVVFT"
gene <39259..>41901
/gene="GPB2"
/locus_tag="YAL056W"
/gene_synonym="KRH1"
/db_xref="GeneID:851243"
mRNA <39259..>41901
/gene="GPB2"
/locus_tag="YAL056W"
/gene_synonym="KRH1"
/product="Gpb2p"
/transcript_id="NM_001178199.2"
/db_xref="GeneID:851243"
CDS 39259..41901
/gene="GPB2"
/locus_tag="YAL056W"
/gene_synonym="KRH1"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16030250]"
/experiment="EXISTENCE:genetic interaction:GO:0001403
invasive growth in response to glucose limitation
[PMID:12150916]"
/experiment="EXISTENCE:genetic interaction:GO:0004862
cAMP-dependent protein kinase inhibitor activity
[PMID:16924114|PMID:16278446]"
/experiment="EXISTENCE:genetic interaction:GO:0007124
pseudohyphal growth [PMID:12150916]"
/experiment="EXISTENCE:genetic interaction:GO:0010255
glucose mediated signaling pathway [PMID:12150916]"
/experiment="EXISTENCE:genetic interaction:GO:0046580
negative regulation of Ras protein signal transduction
[PMID:16793550]"
/experiment="EXISTENCE:mutant phenotype:GO:0001403
invasive growth in response to glucose limitation
[PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:0007124
pseudohyphal growth [PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:0010255 glucose
mediated signaling pathway [PMID:12150916]"
/experiment="EXISTENCE:mutant phenotype:GO:0030437
ascospore formation [PMID:12586695]"
/experiment="EXISTENCE:physical interaction:GO:0032794
GTPase activating protein binding [PMID:16793550]"
/note="Multistep regulator of cAMP-PKA signaling; inhibits
PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP
dependency; inhibits Ras activity through direct
interactions with Ira1p/2p; regulated by G-alpha protein
Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole
genome duplication"
/codon_start=1
/product="Gpb2p"
/protein_id="NP_009345.3"
/db_xref="GeneID:851243"
/db_xref="SGD:S000000052"
/translation="MEISSSPWNDGGYSPYERNRVAVSPFSSALEGEERIETSRSLGD
HCFEPLPYVTNYLSIFALFGKEIFGDKGNVSSRNEYLLKKYYSLKKPFVLRHNGHALK
NPDMPLQRNDILQTNFMVDKFLNRTVRSVNFNNFKIISDMQSKSGRGTKSGTNQNQSA
DAIQNICLPSIPSALPYFQYYRKLLTVNTKEWDILKLHSLWVPKLRKDFKDFSLYGDK
NSLKPIDSHYDEDNTMKKNLFFERSPSRQTLDGKGCASKGYDISSGNMIIPSLFSEDK
LPALTYHCSVELNGNIYIFGGLMPCYSYEEDAPMLNDFFVDGIKNLPPPLLPQVINNP
SMVNNPHLYVASIPSCRFSKPKMGGYIPPPLLCVQGSKLTDRHIFFYGGFEIRTETRG
DENGKYHLKKRLYVNNTGYILDIMSFKFTKIDIIVQPSKYNAYPTMSSRFGHLQISID
NPNRRASVHSSSMNEIHKMGSASMKQGSSITSGRLEKAAVLSSLPHNTVHTVIIFGGY
RQTGDDRYEAMNDLWKIEIPVIRRGKKGYCKFSETANAILLTPSEKDKSDWPEERAFS
AFSVHGTSLMDRSSLDMRLLNNLKNHFVLKPSYISQDRVVSPKPVFPMMVHGTHQDLF
NSGSAAQESPKAGASASSASAASFDPDMDDNLENYIVNPGRKSSSIPMTAIGRQRLIL
SQEKPVGKTVVLHGGSNGLNVLDDMWLMDLECETWTPIETFAKADSSEDGDEKLDSVN
VGLVGHRMESIGRICVCIGGMVQEDVDQFYSENDDEPPRKRKVDTLPLGGNFLNTIDL
STQCWEEHKITLSKKEDDEDRQDSENEDTNSNIVVGVGGTSLQCDKSIILIGGLISRR
SNVKEIYLHGTITKSIFPSVNPSA"
rep_origin 41992..42151
/note="ARS105; Autonomously Replicating Sequence"
/db_xref="SGD:S000121254"
gene <42177..>42719
/gene="PEX22"
/locus_tag="YAL055W"
/gene_synonym="YAF5"
/db_xref="GeneID:851244"
mRNA <42177..>42719
/gene="PEX22"
/locus_tag="YAL055W"
/gene_synonym="YAF5"
/product="ubiquitin-protein transferase activating protein
PEX22"
/transcript_id="NM_001178198.1"
/db_xref="GeneID:851244"
CDS 42177..42719
/gene="PEX22"
/locus_tag="YAL055W"
/gene_synonym="YAF5"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:24390141]"
/experiment="EXISTENCE:direct assay:GO:0031398 positive
regulation of protein ubiquitination [PMID:25162638]"
/experiment="EXISTENCE:direct assay:GO:0097027
ubiquitin-protein transferase activator activity
[PMID:22085930]"
/experiment="EXISTENCE:direct assay:GO:1902499 positive
regulation of protein autoubiquitination [PMID:25162638]"
/experiment="EXISTENCE:mutant phenotype:GO:0016562 protein
import into peroxisome matrix, receptor recycling
[PMID:17452527]"
/note="Putative peroxisomal membrane protein; required for
import of peroxisomal proteins; functionally complements a
Pichia pastoris pex22 mutation"
/codon_start=1
/product="ubiquitin-protein transferase activating protein
PEX22"
/protein_id="NP_009346.1"
/db_xref="GeneID:851244"
/db_xref="SGD:S000000051"
/translation="MPPPSRSRINKTRTLGIVGTAIAVLVTSYYIYQKVTSAKEDNGA
RPPEGDSVKENKKARKSKCIIMSKSIQGLPIKWEEYAADEVVLLVPTSHTDGSMKQAI
GDAFRKTKNEHKIIYCDSMDGLWSCVRRLGKFQCILNSRDFTSSGGSDAAVVPEDIGR
FVKFVVDSDVEDVLIDTLCN"
gene complement(<42881..>45022)
/gene="ACS1"
/locus_tag="YAL054C"
/gene_synonym="FUN44"
/db_xref="GeneID:851245"
mRNA complement(<42881..>45022)
/gene="ACS1"
/locus_tag="YAL054C"
/gene_synonym="FUN44"
/product="acetate--CoA ligase 1"
/transcript_id="NM_001178197.1"
/db_xref="GeneID:851245"
CDS complement(42881..45022)
/gene="ACS1"
/locus_tag="YAL054C"
/gene_synonym="FUN44"
/EC_number="6.2.1.1"
/experiment="EXISTENCE:direct assay:GO:0003987 acetate-CoA
ligase activity [PMID:8910545]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:9711835]"
/experiment="EXISTENCE:direct assay:GO:0006085 acetyl-CoA
biosynthetic process [PMID:8910545]"
/experiment="EXISTENCE:direct assay:GO:0016880
acid-ammonia (or amide) ligase activity [PMID:18305111]"
/experiment="EXISTENCE:mutant phenotype:GO:0019654
pyruvate fermentation to acetate [PMID:1363452]"
/note="Acetyl-coA synthetase isoform; along with Acs2p,
acetyl-CoA synthetase isoform is the nuclear source of
acetyl-coA for histone acetylation; expressed during
growth on nonfermentable carbon sources and under aerobic
conditions; metabolites mediate the formation of filament
assemblies in meiotic cells, storing Acs1p in an inactive
state and promoting efficient return to growth after
starvation"
/codon_start=1
/product="acetate--CoA ligase 1"
/protein_id="NP_009347.1"
/db_xref="GeneID:851245"
/db_xref="SGD:S000000050"
/translation="MSPSAVQSSKLEEQSSEIDKLKAKMSQSAATAQQKKEHEYEHLT
SVKIVPQRPISDRLQPAIATHYSPHLDGLQDYQRLHKESIEDPAKFFGSKATQFLNWS
KPFDKVFIPDPKTGRPSFQNNAWFLNGQLNACYNCVDRHALKTPNKKAIIFEGDEPGQ
GYSITYKELLEEVCQVAQVLTYSMGVRKGDTVAVYMPMVPEAIITLLAISRIGAIHSV
VFAGFSSNSLRDRINDGDSKVVITTDESNRGGKVIETKRIVDDALRETPGVRHVLVYR
KTNNPSVAFHAPRDLDWATEKKKYKTYYPCTPVDSEDPLFLLYTSGSTGAPKGVQHST
AGYLLGALLTMRYTFDTHQEDVFFTAGDIGWITGHTYVVYGPLLYGCATLVFEGTPAY
PNYSRYWDIIDEHKVTQFYVAPTALRLLKRAGDSYIENHSLKSLRCLGSVGEPIAAEV
WEWYSEKIGKNEIPIVDTYWQTESGSHLVTPLAGGVTPMKPGSASFPFFGIDAVVLDP
NTGEELNTSHAEGVLAVKAAWPSFARTIWKNHDRYLDTYLNPYPGYYFTGDGAAKDKD
GYIWILGRVDDVVNVSGHRLSTAEIEAAIIEDPIVAECAVVGFNDDLTGQAVAAFVVL
KNKSSWSTATDDELQDIKKHLVFTVRKDIGPFAAPKLIILVDDLPKTRSGKIMRRILR
KILAGESDQLGDVSTLSNPGIVRHLIDSVKL"
gene <45899..>48250
/gene="FLC2"
/locus_tag="YAL053W"
/db_xref="GeneID:851246"
mRNA <45899..>48250
/gene="FLC2"
/locus_tag="YAL053W"
/product="flavin adenine dinucleotide transporter FLC2"
/transcript_id="NM_001178196.2"
/db_xref="GeneID:851246"
CDS 45899..48250
/gene="FLC2"
/locus_tag="YAL053W"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:16717099]"
/experiment="EXISTENCE:genetic interaction:GO:0030148
sphingolipid biosynthetic process [PMID:27462707]"
/experiment="EXISTENCE:mutant phenotype:GO:0006457 protein
folding [PMID:16717099]"
/experiment="EXISTENCE:mutant phenotype:GO:0009272
fungal-type cell wall biogenesis [PMID:16717099]"
/experiment="EXISTENCE:mutant phenotype:GO:0015230 FAD
transmembrane transporter activity [PMID:16717099]"
/experiment="EXISTENCE:mutant phenotype:GO:0015883 FAD
transport [PMID:16717099]"
/experiment="EXISTENCE:mutant phenotype:GO:0055074 calcium
ion homeostasis [PMID:25573187]"
/experiment="EXISTENCE:mutant phenotype:GO:0055085
transmembrane transport [PMID:16717099]"
/experiment="EXISTENCE:mutant phenotype:GO:0071476
cellular hypotonic response [PMID:25573187]"
/note="Putative calcium channel involved in calcium
release under hypotonic stress; required for uptake of FAD
into endoplasmic reticulum; involved in cell wall
maintenance; targeted to vacuole via AP-3 pathway; FLC2
has a paralog, YOR365C, that arose from the whole genome
duplication"
/codon_start=1
/product="flavin adenine dinucleotide transporter FLC2"
/protein_id="NP_009348.2"
/db_xref="GeneID:851246"
/db_xref="SGD:S000000049"
/translation="MIFLNTFARCLLTCFVLCSGTARSSDTNDTTPASAKHLQTTSLL
TCMDNSQLTASFFDVKFYPDNNTVIFDIDATTTLNGNVTVKAELLTYGLKVLDKTFDL
CSLGQVSLCPLSAGRIDVMSTQVIESSITKQFPGIAYTIPDLDAQVRVVAYAQNDTEF
ETPLACVQAILSNGKTVQTKYAAWPIAAISGVGVLTSGFVSVIGYSATAAHIASNSIS
LFIYFQNLAITAMMGVSRVPPIAAAWTQNFQWSMGIINTNFMQKIFDWYVQATNGVSN
VVVANKDVLSISVQKRAISMASSSDYNFDTILDDSNLYTTSEKDPSNYSAKILVLRGI
ERVAYLANIELSNFFLTGIVFFLFFLFVVVVSLIFFKALLEVLTRARILKETSNFFQY
RKNWGSIIKGTLFRLSIIAFPQVSLLAIWEFTQVNSPAIVVDAVVILLIITGLLVYGT
IRVFIKGRESLRLYKNPAYLLYSDTYFLNKFGFLYVQFKADKFWWLLPLLSYAFLRSL
FVAVLQNQGKAQAMIIFVIELAYFVCLCWIRPYLDKRTNVFNIAIHLVNLINAFFFLF
FSNLFKQPAVVSSVMAVILFVLNAVFALFLLLFTIVTCTLALLHRNPDVRYQPMKDDR
VSFIPKIQNDFDGKNKNDSELFELRKAVMDTNENEEEKMFRDDTFGKNLNANTNTARL
FDDETSSSSFKQNSSPFDASEVTEQPVQPTSAVMGTGGSFLSPQYQRASSASRTNLAP
NNTSTSSLMKPESSLYLGNSNKSYSHFNNNGSNENARNNNPYL"
gene <48564..>51707
/gene="OAF1"
/locus_tag="YAL051W"
/gene_synonym="YAF1"
/db_xref="GeneID:851247"
mRNA <48564..>51707
/gene="OAF1"
/locus_tag="YAL051W"
/gene_synonym="YAF1"
/product="oleate-activated transcription factor OAF1"
/transcript_id="NM_001178195.2"
/db_xref="GeneID:851247"
CDS 48564..51707
/gene="OAF1"
/locus_tag="YAL051W"
/gene_synonym="YAF1"
/experiment="EXISTENCE:direct assay:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:9288897]"
/experiment="EXISTENCE:direct assay:GO:0001228 DNA-binding
transcription activator activity, RNA polymerase
II-specific [PMID:10428786]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding
[PMID:19158363|PMID:19111667]"
/experiment="EXISTENCE:direct assay:GO:0071400 cellular
response to oleic acid [PMID:10428786|PMID:9288897]"
/experiment="EXISTENCE:direct assay:GO:0089716 Pip2-Oaf1
complex [PMID:9288897]"
/experiment="EXISTENCE:mutant phenotype:GO:0000978 RNA
polymerase II cis-regulatory region sequence-specific DNA
binding [PMID:9288897]"
/experiment="EXISTENCE:mutant phenotype:GO:0001228
DNA-binding transcription activator activity, RNA
polymerase II-specific [PMID:8972187|PMID:10428786]"
/experiment="EXISTENCE:mutant phenotype:GO:0032000
positive regulation of fatty acid beta-oxidation
[PMID:8972187]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:8972187]"
/experiment="EXISTENCE:mutant phenotype:GO:0071400
cellular response to oleic acid
[PMID:9288897|PMID:8972187|PMID:10428786]"
/experiment="EXISTENCE:physical interaction:GO:0089716
Pip2-Oaf1 complex [PMID:8972187]"
/note="Oleate-activated transcription factor; subunit of a
heterodimeric complex with Pip2p, which binds to
oleate-response elements (ORE) in the promoter of genes
involved in beta-oxidation of fatty acids, peroxisome
organization and biogenesis, activating transcription in
the presence of oleate; regulates chromatin silencing at
telomeres; involved in diauxic shift; OAF1 has a paralog,
PIP2, that arose from the whole genome duplication"
/codon_start=1
/product="oleate-activated transcription factor OAF1"
/protein_id="NP_009349.3"
/db_xref="GeneID:851247"
/db_xref="SGD:S000000048"
/translation="MVENSTQKAPHAGNDDNSSTKPYSEAFFLGFNNPTPGLEAEHSS
TSPAPENSETHNRKRNRILFVCQACRKSKTKCDREKPECGRCVKHGLKCVYDVSKQPA
PRIPSKDAIISRLEKDMFYWKDKAMKLLTEREVNESGKRSASPINTNNASGDSPDTKK
QHKMEPIYEQSGNGDINNGTRNDIEINLYRSHPTMIMSKVMKREVKPLSENYIIIQDC
FLKILVTSVFLDTSKNTMIPALTANANITRAQPSVANNLLKLKEMLIRQCQTEDEKNR
VNEFTDRILQNTNSNRNLKIGMLLSMLYNSVGYQYLEDHCPQGGEYSDLLRNLINECE
AILPSYEIIERYKNHFYEYVYPSLPFIELEIFEESLSQTIFPDPNNPSKVQIRMGSTH
LRAKVENLSLLLVILKLSYMSIRFLDHSTADSSFYLSKEIIDKYPIPNDFILLSQRCL
ASENWCACANENIISCLLYIWSFFAFSPEEGDFFLEHPTDVISSLIMMLSTSIGLHRD
PSDFPQLISPSTSDKRTLNHRRILWLSIVTVCSFEASLKGRHSVSPISLMALFLNIKD
PDSLTVYMNRVRGDLSDINNHKLLRIHKFTFKRAQLALLLSDLDNLTMTYYGSFHLHS
IEFIREKIEIFVEENFPIVPLKSVAQDKSDLDDMNVISEMNILSSENSSSFHNRIMNK
LLMLRTSMAVFLHFETLITKDKSIFPFYKKYFMVSCMDALSLINYFNKFFNGEYRHAI
SSLTSFNVTKFIQLALSSTIFSLLGIILRIGLAIHMLSSEVQKLSGTTDPRIKELNTK
VEKFSTLQRDLESALEGIYCSASEHLRFTYFPVFKMLALFDVIVQRMRKGELWHGIFT
MIQMEQMHSRIIKTLSITLGVKLDKKDRLLEELMACNHVANFSVEDIDELNRNIKKEI
QISSGLKPPVNTIDLTNGEPFGNAVPTFTKTWSSSLDNLEKLSSAAAVGQSLDYNSGL
RQGPLAGGGSKEQTPIAGMNNLNNSINATPIVDNSSGSQLPNGFDRGQANNTPFPGYF
GGLDLFDYDFLFGNDFA"
gene complement(<51855..>52595)
/gene="AIM2"
/locus_tag="YAL049C"
/db_xref="GeneID:851248"
mRNA complement(<51855..>52595)
/gene="AIM2"
/locus_tag="YAL049C"
/product="protein AIM2"
/transcript_id="NM_001178194.1"
/db_xref="GeneID:851248"
CDS complement(51855..52595)
/gene="AIM2"
/locus_tag="YAL049C"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/note="Cytoplasmic protein involved in mitochondrial
function or organization; null mutant displays reduced
frequency of mitochondrial genome loss; potential Hsp82p
interactor"
/codon_start=1
/product="protein AIM2"
/protein_id="NP_009350.1"
/db_xref="GeneID:851248"
/db_xref="SGD:S000000047"
/translation="MASNQPGKCCFEGVCHDGTPKGRREEIFGLDTYAAGSTSPKEKV
IVILTDVYGNKFNNVLLTADKFASAGYMVFVPDILFGDAISSDKPIDRDAWFQRHSPE
VTKKIVDGFMKLLKLEYDPKFIGVVGYCFGAKFAVQHISGDGGLANAAAIAHPSFVSI
EEIEAIDSKKPILISAAEEDHIFPANLRHLTEEKLKDNHATYQLDLFSGVAHGFAARG
DISIPAVKYAKEKVLLDQIYWFNHFSNV"
gene complement(<52801..>54789)
/gene="GEM1"
/locus_tag="YAL048C"
/gene_synonym="GON1"
/db_xref="GeneID:851249"
mRNA complement(<52801..>54789)
/gene="GEM1"
/locus_tag="YAL048C"
/gene_synonym="GON1"
/product="ERMES complex Ca(2+)-binding regulatory GTPase
GEM1"
/transcript_id="NM_001178193.1"
/db_xref="GeneID:851249"
CDS complement(52801..54789)
/gene="GEM1"
/locus_tag="YAL048C"
/gene_synonym="GON1"
/experiment="EXISTENCE:direct assay:GO:0005509 calcium ion
binding [PMID:21036903]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:16407407|PMID:15479738]"
/experiment="EXISTENCE:direct assay:GO:0044233
mitochondria-associated endoplasmic reticulum membrane
contact site [PMID:37366411]"
/experiment="EXISTENCE:genetic interaction:GO:0000001
mitochondrion inheritance [PMID:18245340]"
/experiment="EXISTENCE:genetic interaction:GO:0055091
phospholipid homeostasis [PMID:21825164]"
/experiment="EXISTENCE:mutant phenotype:GO:0000001
mitochondrion inheritance [PMID:18245340]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:15479738]"
/experiment="EXISTENCE:mutant phenotype:GO:0010821
regulation of mitochondrion organization [PMID:21825164]"
/experiment="EXISTENCE:mutant phenotype:GO:0015886 heme
transport [PMID:32265272]"
/experiment="EXISTENCE:mutant phenotype:GO:1990456
mitochondrion-endoplasmic reticulum membrane tethering
[PMID:21825164|PMID:23682313]"
/experiment="EXISTENCE:physical interaction:GO:0032865
ERMES complex [PMID:21825164|PMID:21945531]"
/note="Outer mitochondrial membrane GTPase, subunit of the
ERMES complex; potential regulatory subunit of the ERMES
complex that links the ER to mitochondria and may promote
inter-organellar calcium and phospholipid exchange as well
as coordinating mitochondrial DNA replication and growth;
cells lacking Gem1p contain collapsed, globular, or
grape-like mitochondria; ortholog of metazoan Miro
GTPases"
/codon_start=1
/product="ERMES complex Ca(2+)-binding regulatory GTPase
GEM1"
/protein_id="NP_009351.1"
/db_xref="GeneID:851249"
/db_xref="SGD:S000000046"
/translation="MTKETIRVVICGDEGVGKSSLIVSLTKAEFIPTIQDVLPPISIP
RDFSSSPTYSPKNTVLIDTSDSDLIALDHELKSADVIWLVYCDHESYDHVSLFWLPHF
RSLGLNIPVILCKNKCDSISNVNANAMVVSENSDDDIDTKVEDEEFIPILMEFKEIDT
CIKTSAKTQFDLNQAFYLCQRAITHPISPLFDAMVGELKPLAVMALKRIFLLSDLNQD
SYLDDNEILGLQKKCFNKSIDVNELNFIKDLLLDISKHDQEYINRKLYVPGKGITKDG
FLVLNKIYAERGRHETTWAILRTFHYTDSLCINDKILHPRLVVPDTSSVELSPKGYRF
LVDIFLKFDIDNDGGLNNQELHRLFKCTPGLPKLWTSTNFPFSTVVNNKGCITLQGWL
AQWSMTTFLNYSTTTAYLVYFGFQEDARLALQVTKPRKMRRRSGKLYRSNINDRKVFN
CFVIGKPCCGKSSLLEAFLGRSFSEEYSPTIKPRIAVNSLELKGGKQYYLILQELGEQ
EYAILENKDKLKECDVICLTYDSSDPESFSYLVSLLDKFTHLQDLPLVFVASKADLDK
QQQRCQIQPDELADELFVNHPLHISSRWLSSLNELFIKITEAALDPGKNTPGLPEETA
AKDVDYRQTALIFGSTVGFVALCSFTLMKLFKSSKFSK"
gene complement(<54989..>56857)
/gene="SPC72"
/locus_tag="YAL047C"
/gene_synonym="LDB4"
/db_xref="GeneID:851250"
mRNA complement(<54989..>56857)
/gene="SPC72"
/locus_tag="YAL047C"
/gene_synonym="LDB4"
/product="gamma-tubulin complex subunit SPC72"
/transcript_id="NM_001178192.2"
/db_xref="GeneID:851250"
CDS complement(54989..56857)
/gene="SPC72"
/locus_tag="YAL047C"
/gene_synonym="LDB4"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:9382872]"
/experiment="EXISTENCE:direct assay:GO:0005824 outer
plaque of spindle pole body [PMID:9670012]"
/experiment="EXISTENCE:direct assay:GO:0005876 spindle
microtubule [PMID:9382872]"
/experiment="EXISTENCE:direct assay:GO:0044877
protein-containing complex binding
[PMID:9670012|PMID:12970190]"
/experiment="EXISTENCE:direct assay:GO:0051010 microtubule
plus-end binding [PMID:12970190]"
/experiment="EXISTENCE:direct assay:GO:0061499 outer
plaque of mitotic spindle pole body [PMID:9585415]"
/experiment="EXISTENCE:direct assay:GO:0099609 microtubule
lateral binding [PMID:9382872]"
/experiment="EXISTENCE:direct assay:GO:1905721 mitotic
spindle astral microtubule end [PMID:12970190]"
/experiment="EXISTENCE:genetic interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:17967947]"
/experiment="EXISTENCE:mutant phenotype:GO:0007020
microtubule nucleation [PMID:18573877]"
/experiment="EXISTENCE:mutant phenotype:GO:0030953 astral
microtubule organization [PMID:12970190]"
/experiment="EXISTENCE:mutant phenotype:GO:0031122
cytoplasmic microtubule organization [PMID:9606209]"
/experiment="EXISTENCE:mutant phenotype:GO:1990734 astral
microtubule anchoring at mitotic spindle pole body
[PMID:12970190]"
/experiment="EXISTENCE:physical interaction:GO:0031578
mitotic spindle orientation checkpoint signaling
[PMID:17967947]"
/experiment="EXISTENCE:physical interaction:GO:0110121
gamma-tubulin complex localization to cytoplasmic side of
mitotic spindle pole body [PMID:9670012]"
/note="Gamma-tubulin small complex (gamma-TuSC) receptor;
recruits the gamma-TuSC complex to the cytoplasmic side of
the SPB, connecting nuclear microtubules to the SPB;
involved in astral microtubule formation, stabilization,
and with Stu2p, anchoring astral MTs at the cytoplasmic
face of the SPB, and regulating plus-end MT dynamics;
regulated by Cdc5 kinase"
/codon_start=1
/product="gamma-tubulin complex subunit SPC72"
/protein_id="NP_009352.2"
/db_xref="GeneID:851250"
/db_xref="SGD:S000000045"
/translation="MVRRWIPSGRHLRNNDNTGDDDDSEFTNSMDSGMSIPSLRDSMT
TRSSHNDPIKPALMNDSNKVKNLEKELTNAKIKIQVLYEYIRRIPNKDGNAPSLGNDT
DFRNSIIEGLNLEINKLKQDLKAKEVEYQDTLQFVQENLENSESIVNTINHLLSFILT
HFNEQDENAHLLDKEERETLEETLELSSDYVLEKMDTLSKFIIQFLQDFLHSKSRAES
KQDKEEFLSLAQSSPAGSQLESRDSPSSKEENTDGGYQNDEIHDSNNHIDTENVMANS
TSLPISAVESRFEKTLDTQLEIVIENLHKEYDQFINSIRLKFEKSQKLEKIIASKLNE
QSHLLDSLELEENSSSVIEKQDHLISQLKEKIESQSVLINNLEKLKEDIIKMKQNEKV
LTKELETQTKINKLKENNWDSYINDLEKQINDLQIDKSEEFHVIQNQLDKLDLENYQL
KNQLNTLDNQKLILSQYESNFIKFNQNLLLHLDSIFNILQKILQESSIAQFDRKMKSI
KSVPNALKNLNLIQPKLESLYTFIETALESIINSYISSLISMETPEQPHQQGNELTAT
PNKELTLRIEELQRRWISERERRKLDANASEARIKALEQENESLRSKLFNLSINNP"
gene complement(<57029..>57385)
/gene="BOL3"
/locus_tag="YAL046C"
/gene_synonym="AIM1"
/db_xref="GeneID:851251"
mRNA complement(<57029..>57385)
/gene="BOL3"
/locus_tag="YAL046C"
/gene_synonym="AIM1"
/product="Bol3p"
/transcript_id="NM_001178191.1"
/db_xref="GeneID:851251"
CDS complement(57029..57385)
/gene="BOL3"
/locus_tag="YAL046C"
/gene_synonym="AIM1"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:27532772]"
/experiment="EXISTENCE:genetic interaction:GO:0051604
protein maturation [PMID:27532772|PMID:27532773]"
/experiment="EXISTENCE:mutant phenotype:GO:0051604 protein
maturation [PMID:27532772]"
/experiment="EXISTENCE:physical interaction:GO:0051604
protein maturation [PMID:27532773]"
/note="Protein involved in Fe-S cluster transfer to
mitochondrial clients; protects [4Fe-4S] clusters from
damage due to oxidative stress by acting along with Nfu1p
at a late step in the transfer of [4Fe-4S] clusters from
the ISA complex to mitochondrial client proteins like
lipoate synthase and succinate dehydrogenase; sequence
similarity to human BOLA family member, BOLA3, mutations
of which are associated with Multiple Mitochondria
Dysfunctions Syndrome (MMDS2)"
/codon_start=1
/product="Bol3p"
/protein_id="NP_009353.1"
/db_xref="GeneID:851251"
/db_xref="SGD:S000000044"
/translation="MKLPQTMLRSISVKHVRWPRILTGSKLWYSTQMAMTPEEKMITD
KLQQELEPEVCKVQDVSGGCGSMFAINITSKKFNGLSLIKQHQLVNRILRDDISRWHG
LQLTTKKSTGKGPASS"
gene <57518..>57850
/gene="BOL1"
/locus_tag="YAL044W-A"
/db_xref="GeneID:851252"
mRNA <57518..>57850
/gene="BOL1"
/locus_tag="YAL044W-A"
/product="Bol1p"
/transcript_id="NM_001184453.1"
/db_xref="GeneID:851252"
CDS 57518..57850
/gene="BOL1"
/locus_tag="YAL044W-A"
/experiment="EXISTENCE:direct assay:GO:0005759
mitochondrial matrix [PMID:27532772]"
/experiment="EXISTENCE:genetic interaction:GO:0044572
[4Fe-4S] cluster assembly [PMID:27532773]"
/experiment="EXISTENCE:genetic interaction:GO:0051604
protein maturation [PMID:27532772]"
/experiment="EXISTENCE:mutant phenotype:GO:0044572
[4Fe-4S] cluster assembly [PMID:27532773]"
/experiment="EXISTENCE:physical interaction:GO:0044572
[4Fe-4S] cluster assembly [PMID:27532773]"
/note="Mitochondrial matrix protein involved in Fe-S
cluster biogenesis; facilitates [4Fe-2S] cluster inception
into mitochondrial proteins such as lipoate synthase and
succinate dehydrogenase; interacts and may function with
Grx5p at an early step in Fe-S cluster biosynthesis; forms
dimeric complexes with Grx5p and Nfu1p that alter the
stability of shared Fe/S clusters; sequence similarity to
human BOLA family member, BOLA1 and S. pombe uvi31, a
putative DNA repair protein"
/codon_start=1
/product="Bol1p"
/protein_id="NP_075206.1"
/db_xref="GeneID:851252"
/db_xref="SGD:S000007586"
/translation="MFKRAMSTDGPVARTILKRLECGFPDYKNFAFGLYNDSHKHKGH
AGVQGNVSAETHFRIEMVSKKFEGLKLPQRHRMVYSLLQDEMAQANGIHALQLSLKTP
QEYESKAK"
gene complement(<57950..>58462)
/gene="GCV3"
/locus_tag="YAL044C"
/db_xref="GeneID:851254"
mRNA complement(<57950..>58462)
/gene="GCV3"
/locus_tag="YAL044C"
/product="glycine decarboxylase subunit H"
/transcript_id="NM_001178189.1"
/db_xref="GeneID:851254"
CDS complement(57950..58462)
/gene="GCV3"
/locus_tag="YAL044C"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:19570983|PMID:24769239|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0031405 lipoic acid
binding [PMID:32974306]"
/experiment="EXISTENCE:genetic interaction:GO:0006730
one-carbon metabolic process [PMID:10871621]"
/experiment="EXISTENCE:mutant phenotype:GO:0005960 glycine
cleavage complex [PMID:9020168]"
/experiment="EXISTENCE:mutant phenotype:GO:0006546 glycine
catabolic process [PMID:9020168]"
/experiment="EXISTENCE:mutant phenotype:GO:0009249 protein
lipoylation [PMID:19570983]"
/note="H subunit of the mitochondrial glycine
decarboxylase complex; glycine decarboxylase is required
for the catabolism of glycine to 5,10-methylene-THF; also
required for all protein lipoylation; expression is
regulated by levels of 5,10-methylene-THF"
/codon_start=1
/product="glycine decarboxylase subunit H"
/protein_id="NP_009355.3"
/db_xref="GeneID:851254"
/db_xref="SGD:S000000042"
/translation="MLRTTRLWTTRMPTVSKLFLRNSSGNALNKNKLPFLYSSQGPQA
VRYTSQHEWIAVHQDKTAFVGITKYATDALGDATYVELPEVGTEIAQGESLGSIESVK
SASEIYQPADGTVEEINTNLEENPGVVNEDPMGDGWLVKMKLGEGVNVEQVEGLMSLE
QYEKTLVHDD"
gene complement(<58695..>61052)
/gene="PTA1"
/locus_tag="YAL043C"
/gene_synonym="FUN39"
/db_xref="GeneID:851255"
mRNA complement(<58695..>61052)
/gene="PTA1"
/locus_tag="YAL043C"
/gene_synonym="FUN39"
/product="RNA-processing protein PTA1"
/transcript_id="NM_001178188.1"
/db_xref="GeneID:851255"
CDS complement(58695..61052)
/gene="PTA1"
/locus_tag="YAL043C"
/gene_synonym="FUN39"
/experiment="EXISTENCE:curator inference:GO:0003723 RNA
binding [PMID:10523662]"
/experiment="EXISTENCE:direct assay:GO:0005847 mRNA
cleavage and polyadenylation specificity factor complex
[PMID:12819204|PMID:9099738|PMID:10523662]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:1508188]"
/experiment="EXISTENCE:physical interaction:GO:0030846
termination of RNA polymerase II transcription,
poly(A)-coupled [PMID:12819204]"
/note="Subunit of holo-CPF; holo-CPF is a multiprotein
complex and functional homolog of mammalian CPSF, required
for the cleavage and polyadenylation of mRNA and snoRNA 3'
ends; involved in pre-tRNA processing; binds to the
phosphorylated CTD of RNAPII"
/codon_start=1
/product="RNA-processing protein PTA1"
/protein_id="NP_009356.1"
/db_xref="GeneID:851255"
/db_xref="SGD:S000000041"
/translation="MSSAEMEQLLQAKTLAMHNNPTEMLPKVLETTASMYHNGNLSKL
KLPLAKFFTQLVLDVVSMDSPIANTERPFIAAQYLPLLLAMAQSTADVLVYKNIVLIM
CASYPLVLDLVAKTSNQEMFDQLCMLKKFVLSHWRTAYPLRATVDDETDVEQWLAQID
QNIGVKLATIKFISEVVLSQTKSPSGNEINSSTIPDNHPVLNKPALESEAKRLLDMLL
NYLIEEQYMVSSVFIGIINSLSFVIKRRPQTTIRILSGLLRFNVDAKFPLEGKSDLNY
KLSKRFVERAYKNFVQFGLKNQIITKSLSSGSGSSIYSKLTKISQTLHVIGEETKSKG
ILNFDPSKGNSKKTLSRQDKLKYISLWKRQLSALLSTLGVSTKTPTPVSAPATGSSTE
NMLDQLKILQKYTLNKASHQGNTFFNNSPKPISNTYSSVYSLMNSSNSNQDVTQLPND
ILIKLSTEAILQMDSTKLITGLSIVASRYTDLMNTYINSVPSSSSSKRKSDDDDDGND
NEEVGNDGPTANSKKIKMETEPLAEEPEEPEDDDRMQKMLQEEESAQEISGDANKSTS
AIKEIAPPFEPDSLTQDEKLKYLSKLTKKLFELSGRQDTTRAKSSSSSSILLDDDDSS
SWLHVLIRLVTRGIEAQEASDLIREELLGFFIQDFEQRVSLIIEWLNEEWFFQTSLHQ
DPSNYKKWSLRVLESLGPFLENKHRRFFIRLMSELPSLQSDHLEALKPICLDPARSSL
GFQTLKFLIMFRPPVQDTVRDLLHQLKQEDEGLHKQCDSLLDRLK"
gene <61316..>62563
/gene="ERV46"
/locus_tag="YAL042W"
/gene_synonym="FUN9"
/db_xref="GeneID:851256"
mRNA <61316..>62563
/gene="ERV46"
/locus_tag="YAL042W"
/gene_synonym="FUN9"
/product="retrograde cargo receptor ERV46"
/transcript_id="NM_001178187.1"
/db_xref="GeneID:851256"
CDS 61316..62563
/gene="ERV46"
/locus_tag="YAL042W"
/gene_synonym="FUN9"
/experiment="EXISTENCE:direct assay:GO:0000139 Golgi
membrane [PMID:11157978]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005789 endoplasmic
reticulum membrane [PMID:11157978]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:11157978]"
/experiment="EXISTENCE:genetic interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:11157978]"
/note="Protein localized to COPII-coated vesicles; forms a
complex with Erv41p; involved in the membrane fusion stage
of transport"
/codon_start=1
/product="retrograde cargo receptor ERV46"
/protein_id="NP_009358.1"
/db_xref="GeneID:851256"
/db_xref="SGD:S000000040"
/translation="MKRSTLLSLDAFAKTEEDVRVRTRAGGLITLSCILTTLFLLVNE
WGQFNSVVTRPQLVVDRDRHAKLELNMDVTFPSMPCDLVNLDIMDDSGEMQLDILDAG
FTMSRLNSEGRPVGDATELHVGGNGDGTAPVNNDPNYCGPCYGAKDQSQNENLAQEEK
VCCQDCDAVRSAYLEAGWAFFDGKNIEQCEREGYVSKINEHLNEGCRIKGSAQINRIQ
GNLHFAPGKPYQNAYGHFHDTSLYDKTSNLNFNHIINHLSFGKPIQSHSKLLGNDKRH
GGAVVATSPLDGRQVFPDRNTHFHQFSYFAKIVPTRYEYLDNVVIETAQFSATFHSRP
LAGGRDKDHPNTLHVRGGIPGMFVFFEMSPLKVINKEQHGQTWSGFILNCITSIGGVL
AVGTVMDKLFYKAQRSIWGKKSQ"
gene <62840..>65404
/gene="CDC24"
/locus_tag="YAL041W"
/gene_synonym="CLS4"
/db_xref="GeneID:851190"
mRNA <62840..>65404
/gene="CDC24"
/locus_tag="YAL041W"
/gene_synonym="CLS4"
/product="Rho family guanine nucleotide exchange factor
CDC24"
/transcript_id="NM_001178186.1"
/db_xref="GeneID:851190"
CDS 62840..65404
/gene="CDC24"
/locus_tag="YAL041W"
/gene_synonym="CLS4"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:10655592]"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity [PMID:8300560]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:10655592]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:10655592|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:10655592]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:10087263]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:10087263]"
/experiment="EXISTENCE:direct assay:GO:0120171
Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]"
/experiment="EXISTENCE:genetic interaction:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:7565673]"
/experiment="EXISTENCE:mutant phenotype:GO:0000282
cellular bud site selection [PMID:7019215]"
/experiment="EXISTENCE:mutant phenotype:GO:0000749
response to pheromone triggering conjugation with cellular
fusion [PMID:9428768]"
/experiment="EXISTENCE:mutant phenotype:GO:0000750
pheromone-dependent signal transduction involved in
conjugation with cellular fusion [PMID:7565673]"
/experiment="EXISTENCE:mutant phenotype:GO:0007096
regulation of exit from mitosis [PMID:12234925]"
/experiment="EXISTENCE:mutant phenotype:GO:0031106 septin
ring organization [PMID:16371506]"
/experiment="EXISTENCE:mutant phenotype:GO:0043577
chemotropism [PMID:9428768]"
/experiment="EXISTENCE:mutant phenotype:GO:0072697 protein
localization to cell cortex [PMID:16325503]"
/experiment="EXISTENCE:physical interaction:GO:0120171
Cdc24p-Far1p-Gbetagamma complex [PMID:10087263]"
/note="Guanine nucleotide exchange factor (GEF) for
Cdc42p; required for polarity establishment and
maintenance, and mutants have morphological defects in bud
formation and shmooing; relocalizes from nucleus to
cytoplasm upon DNA replication stress; thermosensitivity
of the cdc24-4 mutant in the presence of sorbitol is
functionally complemented by human CDC42"
/codon_start=1
/product="Rho family guanine nucleotide exchange factor
CDC24"
/protein_id="NP_009359.1"
/db_xref="GeneID:851190"
/db_xref="SGD:S000000039"
/translation="MAIQTRFASGTSLSDLKPKPSATSISIPMQNVMNKPVTEQDSLF
HICANIRKRLEVLPQLKPFLQLAYQSSEVLSERQSLLLSQKQHQELLKSNGANRDSSD
LAPTLRSSSISTATSLMSMEGISYTNSNPSATPNMEDTLLTFSMGILPITMDCDPVTQ
LSQLFQQGAPLCILFNSVKPQFKLPVIASDDLKVCKKSIYDFILGCKKHFAFNDEELF
TISDVFANSTSQLVKVLEVVETLMNSSPTIFPSKSKTQQIMNAENQHRHQPQQSSKKH
NEYVKIIKEFVATERKYVHDLEILDKYRQQLLDSNLITSEELYMLFPNLGDAIDFQRR
FLISLEINALVEPSKQRIGALFMHSKHFFKLYEPWSIGQNAAIEFLSSTLHKMRVDES
QRFIINNKLELQSFLYKPVQRLCRYPLLVKELLAESSDDNNTKELEAALDISKNIARS
INENQRRTENHQVVKKLYGRVVNWKGYRISKFGELLYFDKVFISTTNSSSEPEREFEV
YLFEKIIILFSEVVTKKSASSLILKKKSSTSASISASNITDNNGSPHHSYHKRHSNSS
SSNNIHLSSSSAAAIIHSSTNSSDNNSNNSSSSSLFKLSANEPKLDLRGRIMIMNLNQ
IIPQNNRSLNITWESIKEQGNFLLKFKNEETRDNWSSCLQQLIHDLKNEQFKARHHSS
TSTTSSTAKSSSMMSPTTTMNTPNHHNSRQTHDSMASFSSSHMKRVSDVLPKRRTTSS
SFESEIKSISENFKNSIPESSILFRISYNNNSNNTSSSEIFTLLVEKVWNFDDLIMAI
NSKISNTHNNNISPITKIKYQDEDGDFVVLGSDEDWNVAKEMLAENNEKFLNIRLY"
gene complement(<65778..>67520)
/gene="CLN3"
/locus_tag="YAL040C"
/gene_synonym="DAF1; FUN10; WHI1"
/db_xref="GeneID:851191"
mRNA complement(<65778..>67520)
/gene="CLN3"
/locus_tag="YAL040C"
/gene_synonym="DAF1; FUN10; WHI1"
/product="cyclin CLN3"
/transcript_id="NM_001178185.1"
/db_xref="GeneID:851191"
CDS complement(65778..67520)
/gene="CLN3"
/locus_tag="YAL040C"
/gene_synonym="DAF1; FUN10; WHI1"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11509671|PMID:10611233|PMID:11792824]"
/experiment="EXISTENCE:direct assay:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:1316273]"
/experiment="EXISTENCE:direct assay:GO:0042144 vacuole
fusion, non-autophagic [PMID:14573462]"
/experiment="EXISTENCE:genetic interaction:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:19823668]"
/experiment="EXISTENCE:genetic interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668]"
/experiment="EXISTENCE:genetic interaction:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:2147225|PMID:2574633]"
/experiment="EXISTENCE:mutant phenotype:GO:0000082 G1/S
transition of mitotic cell cycle [PMID:19823668]"
/experiment="EXISTENCE:mutant phenotype:GO:0001932
regulation of protein phosphorylation
[PMID:8387915|PMID:1316273]"
/experiment="EXISTENCE:mutant phenotype:GO:0005634 nucleus
[PMID:11509671]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:19823668]"
/experiment="EXISTENCE:mutant phenotype:GO:0007033 vacuole
organization [PMID:14573462]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:2907481|PMID:3062366]"
/experiment="EXISTENCE:mutant phenotype:GO:0016538
cyclin-dependent protein serine/threonine kinase regulator
activity [PMID:8387915|PMID:1316273]"
/experiment="EXISTENCE:mutant phenotype:GO:0042144 vacuole
fusion, non-autophagic [PMID:14573462]"
/note="G1 cyclin involved in cell cycle progression;
activates Cdc28p kinase to promote G1 to S phase
transition; plays a role in regulating transcription of
other G1 cyclins, CLN1 and CLN2; regulated by
phosphorylation and proteolysis; acetyl-CoA induces CLN3
transcription in response to nutrient repletion to promote
cell-cycle entry; cell cycle arrest phenotype of the cln1
cln2 cln3 triple null mutant is complemented by any of
human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1"
/codon_start=1
/product="cyclin CLN3"
/protein_id="NP_009360.1"
/db_xref="GeneID:851191"
/db_xref="SGD:S000000038"
/translation="MAILKDTIIRYANARYATASGTSTATAASVSAASCPNLPLLLQK
RRAIASAKSKNPNLVKRELQAHHSAISEYNNDQLDHYFRLSHTERPLYNLTNFNSQPQ
VNPKMRFLIFDFIMYCHTRLNLSTSTLFLTFTILDKYSSRFIIKSYNYQLLSLTALWI
SSKFWDSKNRMATLKVLQNLCCNQYSIKQFTTMEMHLFKSLDWSICQSATFDSYIDIF
LFQSTSPLSPGVVLSAPLEAFIQQKLALLNNAAGTAINKSSSSQGPSLNINEIKLGAI
MLCELASFNLELSFKYDRSLIALGAINLIKLSLNYYNSNLWENINLALEENCQDLDIK
LSEISNTLLDIAMDQNSFPSSFKSKYLNSNKTSLAKSLLDALQNYCIQLKLEEFYRSQ
ELETMYNTIFAQSFDSDSLTCVYSNATTPKSATVSSAATDYFSDHTHLRRLTKDSISP
PFAFTPTSSSSSPSPFNSPYKTSSSMTTPDSASHHSHSGSFSSTQNSFKRSLSIPQNS
SIFWPSPLTPTTPSLMSNRKLLQNLSVRSKRLFPVRPMATAHPCSAPTQLKKRSTSSV
DCDFNDSSNLKKTR"
regulatory complement(67825..67836)
/regulatory_class="other"
/note="Upstream open reading frame (uORF) in 5'
untranslated region of CLN3 gene, regulate translation"
gene complement(<68716..>69525)
/gene="CYC3"
/locus_tag="YAL039C"
/db_xref="GeneID:851192"
mRNA complement(<68716..>69525)
/gene="CYC3"
/locus_tag="YAL039C"
/product="holocytochrome c synthase CYC3"
/transcript_id="NM_001178184.1"
/db_xref="GeneID:851192"
CDS complement(68716..69525)
/gene="CYC3"
/locus_tag="YAL039C"
/EC_number="4.4.1.17"
/experiment="EXISTENCE:direct assay:GO:0004408
holocytochrome-c synthase activity [PMID:3034577]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005758
mitochondrial intermembrane space [PMID:1656231]"
/experiment="EXISTENCE:direct assay:GO:0018063 cytochrome
c-heme linkage [PMID:3034577]"
/note="Cytochrome c heme lyase (holocytochrome c
synthase); attaches heme to apo-cytochrome c (Cyc1p or
Cyc7p) in mitochondrial intermembrane space; human homolog
HCCS implicated in microphthalmia with linear skin defects
(MLS), and can complement yeast null mutant"
/codon_start=1
/product="holocytochrome c synthase CYC3"
/protein_id="NP_009361.1"
/db_xref="GeneID:851192"
/db_xref="SGD:S000000037"
/translation="MGWFWADQKTTGKDIGGAAVSSMSGCPVMHESSSSSPPSSECPV
MQGDNDRINPLNNMPELAASKQPGQKMDLPVDRTISSIPKSPDSNEFWEYPSPQQMYN
AMVRKGKIGGSGEVAEDAVESMVQVHNFLNEGCWQEVLEWEKPHTDESHVQPKLLKFM
GKPGVLSPRARWMHLCGLLFPSHFSQELPFDRHDWIVLRGERKAEQQPPTFKEVRYVL
DFYGGPDDENGMPTFHVDVRPALDSLDNAKDRMTRFLDRMISGPSSSSSAP"
rep_origin 70300..70469
/note="ARS106; Autonomously Replicating Sequence"
/db_xref="SGD:S000118318"
gene <71786..>73288
/gene="CDC19"
/locus_tag="YAL038W"
/gene_synonym="PYK1"
/db_xref="GeneID:851193"
mRNA <71786..>73288
/gene="CDC19"
/locus_tag="YAL038W"
/gene_synonym="PYK1"
/product="pyruvate kinase CDC19"
/transcript_id="NM_001178183.1"
/db_xref="GeneID:851193"
CDS 71786..73288
/gene="CDC19"
/locus_tag="YAL038W"
/gene_synonym="PYK1"
/EC_number="2.7.1.40"
/experiment="EXISTENCE:direct assay:GO:0004743 pyruvate
kinase activity [PMID:12063246]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:27385335|PMID:14718557]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:mutant phenotype:GO:0004743
pyruvate kinase activity [PMID:323230]"
/experiment="EXISTENCE:mutant phenotype:GO:0006096
glycolytic process [PMID:323230]"
/note="Pyruvate kinase; functions as a homotetramer in
glycolysis to convert phosphoenolpyruvate to pyruvate, the
input for aerobic (TCA cycle) or anaerobic (glucose
fermentation) respiration; regulated via allosteric
activation by fructose bisphosphate; CDC19 has a paralog,
PYK2, that arose from the whole genome duplication"
/codon_start=1
/product="pyruvate kinase CDC19"
/protein_id="NP_009362.1"
/db_xref="GeneID:851193"
/db_xref="SGD:S000000036"
/translation="MSRLERLTSLNVVAGSDLRRTSIIGTIGPKTNNPETLVALRKAG
LNIVRMNFSHGSYEYHKSVIDNARKSEELYPGRPLAIALDTKGPEIRTGTTTNDVDYP
IPPNHEMIFTTDDKYAKACDDKIMYVDYKNITKVISAGRIIYVDDGVLSFQVLEVVDD
KTLKVKALNAGKICSHKGVNLPGTDVDLPALSEKDKEDLRFGVKNGVHMVFASFIRTA
NDVLTIREVLGEQGKDVKIIVKIENQQGVNNFDEILKVTDGVMVARGDLGIEIPAPEV
LAVQKKLIAKSNLAGKPVICATQMLESMTYNPRPTRAEVSDVGNAILDGADCVMLSGE
TAKGNYPINAVTTMAETAVIAEQAIAYLPNYDDMRNCTPKPTSTTETVAASAVAAVFE
QKAKAIIVLSTSGTTPRLVSKYRPNCPIILVTRCPRAARFSHLYRGVFPFVFEKEPVS
DWTDDVEARINFGIEKAKEFGILKKGDTYVSIQGFKAGAGHSNTLQVSTV"
gene complement(<73426..>73518)
/locus_tag="YAL037C-A"
/db_xref="GeneID:1466428"
mRNA complement(<73426..>73518)
/locus_tag="YAL037C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184629.1"
/db_xref="GeneID:1466428"
CDS complement(73426..73518)
/locus_tag="YAL037C-A"
/note="hypothetical protein"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878040.1"
/db_xref="GeneID:1466428"
/db_xref="SGD:S000028732"
/translation="MSISFPKMQHLIVMTTIGDKKVNNNIILFL"
gene <74020..>74823
/locus_tag="YAL037W"
/db_xref="GeneID:851194"
mRNA <74020..>74823
/locus_tag="YAL037W"
/product="uncharacterized protein"
/transcript_id="NM_001178182.1"
/db_xref="GeneID:851194"
CDS 74020..74823
/locus_tag="YAL037W"
/note="hypothetical protein; YAL037W has a paralog,
YOR342C, that arose from the whole genome duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_009363.1"
/db_xref="GeneID:851194"
/db_xref="SGD:S000000035"
/translation="MDMEIEDSSPIDDLKLQKLDTNVYFGPCEILTQPILLQYENIKF
IIGVNLSTEKIASFYTQYFRNSNSVVVNLCSPTTAAVATKKAAIDLYIRNNTILLQKF
VGQYLQMGKKIKTSLTQAQTDTIQSLPQFCNSNVLSGEPLVQYQAFNDLLALFKSFSH
FGNILVISSHSYDCALLKFLISRVMTYYPLVTIQDSLQYMKATLNISISTSDEFDILN
DKELWEFGQTQEILKRRQTSSVKRRCVNLPENSTIDNRMLMGTTKRGRF"
gene complement(<75043..>76152)
/gene="RBG1"
/locus_tag="YAL036C"
/gene_synonym="FUN11"
/db_xref="GeneID:851195"
mRNA complement(<75043..>76152)
/gene="RBG1"
/locus_tag="YAL036C"
/gene_synonym="FUN11"
/product="GTP-binding protein RBG1"
/transcript_id="NM_001178181.1"
/db_xref="GeneID:851195"
CDS complement(75043..76152)
/gene="RBG1"
/locus_tag="YAL036C"
/gene_synonym="FUN11"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:23002146]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005777 peroxisome
[PMID:36164978]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:genetic interaction:GO:0002181
cytoplasmic translation [PMID:21076151]"
/experiment="EXISTENCE:genetic interaction:GO:1903833
positive regulation of cellular response to amino acid
starvation [PMID:23899355]"
/note="Member of the DRG family of GTP-binding proteins;
associates with translating ribosomes; interacts with
Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid;
localizes to cytosol and peroxisomes"
/codon_start=1
/product="GTP-binding protein RBG1"
/protein_id="NP_009364.1"
/db_xref="GeneID:851195"
/db_xref="SGD:S000000034"
/translation="MSTTVEKIKAIEDEMARTQKNKATSFHLGQLKAKLAKLRRELLT
SASSGSGGGAGIGFDVARTGVASVGFVGFPSVGKSTLLSKLTGTESEAAEYEFTTLVT
VPGVIRYKGAKIQMLDLPGIIDGAKDGRGRGKQVIAVARTCNLLFIILDVNKPLHHKQ
IIEKELEGVGIRLNKTPPDILIKKKEKGGISITNTVPLTHLGNDEIRAVMSEYRINSA
EIAFRCDATVDDLIDVLEASSRRYMPAIYVLNKIDSLSIEELELLYRIPNAVPISSGQ
DWNLDELLQVMWDRLNLVRIYTKPKGQIPDFTDPVVLRSDRCSVKDFCNQIHKSLVDD
FRNALVYGSSVKHQPQYVGLSHILEDEDVVTILKK"
gene <76427..>79435
/gene="FUN12"
/locus_tag="YAL035W"
/gene_synonym="yIF2"
/db_xref="GeneID:851196"
mRNA <76427..>79435
/gene="FUN12"
/locus_tag="YAL035W"
/gene_synonym="yIF2"
/product="translation initiation factor eIF5B"
/transcript_id="NM_001178180.1"
/db_xref="GeneID:851196"
CDS 76427..79435
/gene="FUN12"
/locus_tag="YAL035W"
/gene_synonym="yIF2"
/EC_number="3.6.5.3"
/experiment="EXISTENCE:direct assay:GO:0001732 formation
of cytoplasmic translation initiation complex
[PMID:12008673]"
/experiment="EXISTENCE:direct assay:GO:0003743 translation
initiation factor activity [PMID:12008673|PMID:9624054]"
/experiment="EXISTENCE:direct assay:GO:0003924 GTPase
activity
[PMID:17189426|PMID:16461768|PMID:19029250|PMID:12507428]"
/experiment="EXISTENCE:direct assay:GO:0005525 GTP binding
[PMID:21335519|PMID:17189426]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0010494 cytoplasmic
stress granule [PMID:26777405]"
/experiment="EXISTENCE:direct assay:GO:0022627 cytosolic
small ribosomal subunit [PMID:9624054]"
/experiment="EXISTENCE:direct assay:GO:0031369 translation
initiation factor binding [PMID:10982835]"
/experiment="EXISTENCE:direct assay:GO:0033290 eukaryotic
48S preinitiation complex [PMID:17242201]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:21335519|PMID:12507428]"
/experiment="EXISTENCE:direct assay:GO:0070181 small
ribosomal subunit rRNA binding [PMID:19029250]"
/experiment="EXISTENCE:mutant phenotype:GO:0000462
maturation of SSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:22751017]"
/experiment="EXISTENCE:mutant phenotype:GO:0001732
formation of cytoplasmic translation initiation complex
[PMID:12507428|PMID:17189426|PMID:17242201]"
/experiment="EXISTENCE:mutant phenotype:GO:0003924 GTPase
activity [PMID:12471154]"
/experiment="EXISTENCE:mutant phenotype:GO:0006446
regulation of translational initiation [PMID:12507428]"
/experiment="EXISTENCE:mutant phenotype:GO:0042255
ribosome assembly [PMID:22770215]"
/experiment="EXISTENCE:mutant phenotype:GO:0042256
cytosolic ribosome assembly [PMID:12507428]"
/note="Translation initiation factor eIF5B; GTPase that
promotes Met-tRNAiMet binding to ribosomes and ribosomal
subunit joining; promotes GTP-dependent maturation of 18S
rRNA by Nob1p; protein abundance increases in response to
DNA replication stress; homolog of bacterial IF2"
/codon_start=1
/product="translation initiation factor eIF5B"
/protein_id="NP_009365.1"
/db_xref="GeneID:851196"
/db_xref="SGD:S000000033"
/translation="MAKKSKKNQQNYWDEEFEEDAAQNEEISATPTPNPESSAGADDT
SREASASAEGAEAIEGDFMSTLKQSKKKQEKKVIEEKKDGKPILKSKKEKEKEKKEKE
KQKKKEQAARKKAQQQAQKEKNKELNKQNVEKAAAEKAAAEKSQKSKGESDKPSASAK
KPAKKVPAGLAALRRQLELKKQLEEQEKLEREEEERLEKEEEERLANEEKMKEEAKAA
KKEKEKAKREKRKAEGKLLTRKQKEEKKLLERRRAALLSSGNVKVAGLAKKDGEENKP
KKVVYSKKKKRTTQENASEAIKSDSKKDSEVVPDDELKESEDVLIDDWENLALGDDDE
EGTNEETQESTASHENEDQNQGEEEEEGEEEEEEEEERAHVHEVAKSTPAATPAATPT
PSSASPNKKDLRSPICCILGHVDTGKTKLLDKIRQTNVQGGEAGGITQQIGATYFPID
AIKAKTKVMAEYEKQTFDVPGLLVIDTPGHESFSNLRSRGSSLCNIAILVIDIMHGLE
QQTIESIKLLRDRKAPFVVALNKIDRLYDWKAIPNNSFRDSFAKQSRAVQEEFQSRYS
KIQLELAEQGLNSELYFQNKNMSKYVSIVPTSAVTGEGVPDLLWLLLELTQKRMSKQL
MYLSHVEATILEVKVVEGFGTTIDVILSNGYLREGDRIVLCGMNGPIVTNIRALLTPQ
PLRELRLKSEYVHHKEVKAALGVKIAANDLEKAVSGSRLLVVGPEDDEDELMDDVMDD
LTGLLDSVDTTGKGVVVQASTLGSLEALLDFLKDMKIPVMSIGLGPVYKRDVMKASTM
LEKAPEYAVMLCFDVKVDKEAEQYAEQEGIKIFNADVIYHLFDSFTAYQEKLLEERRK
DFLDYAIFPCVLQTLQIINKRGPMIIGVDVLEGTLRVGTPICAVKTDPTTKERQTLIL
GKVISLEINHQPVQEVKKGQTAAGVAVRLEDPSGQQPIWGRHVDENDTLYSLVSRRSI
DTLKDKAFRDQVARSDWLLLKKLKVVFGIE"
gene <79718..>80587
/gene="MTW1"
/locus_tag="YAL034W-A"
/gene_synonym="DSN3; NSL2"
/db_xref="GeneID:851197"
mRNA <79718..>80587
/gene="MTW1"
/locus_tag="YAL034W-A"
/gene_synonym="DSN3; NSL2"
/product="MIND complex subunit MTW1"
/transcript_id="NM_001178179.1"
/db_xref="GeneID:851197"
CDS 79718..80587
/gene="MTW1"
/locus_tag="YAL034W-A"
/gene_synonym="DSN3; NSL2"
/experiment="EXISTENCE:direct assay:GO:0000444 MIS12/MIND
type complex [PMID:14633972]"
/experiment="EXISTENCE:direct assay:GO:0000922 spindle
pole [PMID:12455957]"
/experiment="EXISTENCE:direct assay:GO:0007059 chromosome
segregation [PMID:12455957]"
/experiment="EXISTENCE:mutant phenotype:GO:0034501 protein
localization to kinetochore [PMID:19893618]"
/note="Essential component of the MIND kinetochore
complex; joins kinetochore subunits contacting DNA to
those contacting microtubules; critical to kinetochore
assembly; complex consists of Mtw1p Including
Nnf1p-Nsl1p-Dsn1p (MIND)"
/codon_start=1
/product="MIND complex subunit MTW1"
/protein_id="NP_009367.1"
/db_xref="GeneID:851197"
/db_xref="SGD:S000000032"
/translation="MSAPTMRSTSILTEHLGYPPISLVDDIINAVNEIMYKCTAAMEK
YLLSKSKIGEEDYGEEIKSGVAKLESLLENSVDKNFDKLELYVLRNVLRIPEEYLDAN
VFRLENQKDLVIVDENELKKSEEKLREKVNDVELAFKKNEMLLKRVTKVKRLLFTIRG
FKQKLNELLKCKDDVQLQKILESLKPIDDTMTLLTDSLRKLYVDSESTSSTEEVEALL
QRLKTNGKQNNKDFRTRYIDIRTNNVLRKLGLLGDKEDEKQSAKPDARTQAGDIVSID
IEEPQLDLLDDVL"
gene complement(<80710..>81951)
/gene="FUN19"
/locus_tag="YAL034C"
/db_xref="GeneID:851199"
mRNA complement(<80710..>81951)
/gene="FUN19"
/locus_tag="YAL034C"
/product="Fun19p"
/transcript_id="NM_001180035.1"
/db_xref="GeneID:851199"
CDS complement(80710..81951)
/gene="FUN19"
/locus_tag="YAL034C"
/note="Non-essential hypothetical protein; expression
induced in response to heat stress; FUN19 has a paralog,
YOR338W, that arose from the whole genome duplication"
/codon_start=1
/product="Fun19p"
/protein_id="NP_009368.2"
/db_xref="GeneID:851199"
/db_xref="SGD:S000002134"
/translation="MGLYSPESEKSQLNMNYIGKDDSQSIFRRLNQNLKASNNNNDSN
KNGLNMSDYSNNSPYGRSYDVRINQNSQNNGNGCFSGSIDSLVDEHIIPSPPLSPKLE
SKISHNGSPRMASSVLVGSTPKGAVENVLFVKPVWPNGLSRKRYRYATYGFLSQYKIF
SNLAQPYSKNIINRYNNLAYNARHKYSKYNDDMTPPPLPSSSSRLPSPLASPNLNRQA
RYNMRKQALYNNNLGKFESDTEWIPRKRKVYSPQRRTMTTSPHRAKKFSPSASTPHTN
IASIEAIHDAPQYIPNVSWKKLPDYSPPLSTLPTDSNKSLKIEWKGSPMDLSTDPLRN
ELHPAELVLAQTLRLPCDLYLDSKRRLFLEKVYRLKKGLPFRRTDAQKACRIDVNKAS
RLFQAFEKVGWLQDSNFTKYL"
gene <82706..>83227
/gene="POP5"
/locus_tag="YAL033W"
/gene_synonym="FUN53"
/db_xref="GeneID:851200"
mRNA <82706..>83227
/gene="POP5"
/locus_tag="YAL033W"
/gene_synonym="FUN53"
/product="RNA-binding protein POP5"
/transcript_id="NM_001178178.1"
/db_xref="GeneID:851200"
CDS 82706..83227
/gene="POP5"
/locus_tag="YAL033W"
/gene_synonym="FUN53"
/EC_number="3.1.26.5"
/experiment="EXISTENCE:direct assay:GO:0000171
ribonuclease MRP activity [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0000172
ribonuclease MRP complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0000294
nuclear-transcribed mRNA catabolic process, RNase
MRP-dependent [PMID:14729943]"
/experiment="EXISTENCE:direct assay:GO:0003723 RNA binding
[PMID:23700311]"
/experiment="EXISTENCE:direct assay:GO:0004526
ribonuclease P activity [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0005655 nucleolar
ribonuclease P complex [PMID:9620854]"
/experiment="EXISTENCE:direct assay:GO:0034965 intronic
box C/D snoRNA processing [PMID:18713869]"
/experiment="EXISTENCE:mutant phenotype:GO:0000171
ribonuclease MRP activity [PMID:9620854]"
/experiment="EXISTENCE:mutant phenotype:GO:0004526
ribonuclease P activity [PMID:9620854]"
/experiment="EXISTENCE:mutant phenotype:GO:0006364 rRNA
processing [PMID:9620854]"
/experiment="EXISTENCE:mutant phenotype:GO:0008033 tRNA
processing [PMID:9620854]"
/experiment="EXISTENCE:physical interaction:GO:0000172
ribonuclease MRP complex [PMID:17881380]"
/note="Subunit of both RNase MRP and nuclear RNase P;
RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves
tRNA precursors to generate mature 5' ends and facilitates
turnover of nuclear RNAs"
/codon_start=1
/product="RNA-binding protein POP5"
/protein_id="NP_009369.1"
/db_xref="GeneID:851200"
/db_xref="SGD:S000000031"
/translation="MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVS
IKSILQEIRRSLSLNLGDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALML
MSKIGDVDGLIVNPVKVSGTIKKIEQFAMRRNSKILNIIKCSQSSHLSDNDFIINDFK
KIGRENENENEDD"
gene complement(<83335..>84474)
/gene="PRP45"
/locus_tag="YAL032C"
/gene_synonym="FUN20"
/db_xref="GeneID:851201"
mRNA complement(<83335..>84474)
/gene="PRP45"
/locus_tag="YAL032C"
/gene_synonym="FUN20"
/product="mRNA splicing protein PRP45"
/transcript_id="NM_001178177.1"
/db_xref="GeneID:851201"
CDS complement(83335..84474)
/gene="PRP45"
/locus_tag="YAL032C"
/gene_synonym="FUN20"
/experiment="EXISTENCE:curator inference:GO:0000384 first
spliceosomal transesterification activity [PMID:12554883]"
/experiment="EXISTENCE:curator inference:GO:0000386 second
spliceosomal transesterification activity [PMID:12554883]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12359070]"
/experiment="EXISTENCE:direct assay:GO:0071006 U2-type
catalytic step 1 spliceosome [PMID:12554883]"
/experiment="EXISTENCE:direct assay:GO:0071007 U2-type
catalytic step 2 spliceosome [PMID:12554883]"
/experiment="EXISTENCE:direct assay:GO:0071014 post-mRNA
release spliceosomal complex [PMID:12554883]"
/experiment="EXISTENCE:genetic interaction:GO:0000350
generation of catalytic spliceosome for second
transesterification step [PMID:19016306]"
/experiment="EXISTENCE:mutant phenotype:GO:0000350
generation of catalytic spliceosome for second
transesterification step [PMID:19016306]"
/note="Protein required for pre-mRNA splicing; associates
with the spliceosome and interacts with splicing factors
Prp22p and Prp46p; orthologous to human transcriptional
coactivator SKIP and can activate transcription of a
reporter gene"
/codon_start=1
/product="mRNA splicing protein PRP45"
/protein_id="NP_009370.1"
/db_xref="GeneID:851201"
/db_xref="SGD:S000000030"
/translation="MFSNRLPPPKHSQGRVSTALSSDRVEPAILTDQIAKNVKLDDFI
PKRQSNFELSVPLPTKAEIQECTARTKSYIQRLVNAKLANSNNRASSRYVTETHQAPA
NLLLNNSHHIEVVSKQMDPLLPRFVGKKARKVVAPTENDEVVPVLHMDGSNDRGEADP
NEWKIPAAVSNWKNPNGYTVALERRVGKALDNENNTINDGFMKLSEALENADKKARQE
IRSKMELKRLAMEQEMLAKESKLKELSQRARYHNGTPQTGAIVKPKKQTSTVARLKEL
AYSQGRDVSEKIILGAAKRSEQPDLQYDSRFFTRGANASAKRHEDQVYDNPLFVQQDI
ESIYKTNYEKLDEAVNVKSEGASGSHGPIQFTKAESDDKSDNYGA"
gene complement(<84749..>87031)
/gene="GIP4"
/locus_tag="YAL031C"
/gene_synonym="FUN21"
/db_xref="GeneID:851202"
mRNA complement(<84749..>87031)
/gene="GIP4"
/locus_tag="YAL031C"
/gene_synonym="FUN21"
/product="protein phosphatase regulator GIP4"
/transcript_id="NM_001178176.1"
/db_xref="GeneID:851202"
CDS complement(84749..87031)
/gene="GIP4"
/locus_tag="YAL031C"
/gene_synonym="FUN21"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0008157 protein
phosphatase 1 binding [PMID:16537909]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:16537909]"
/experiment="EXISTENCE:mutant phenotype:GO:0019888 protein
phosphatase regulator activity [PMID:16537909]"
/note="Cytoplasmic protein that regulates protein
phosphatase 1 Glc7p; protein overexpression relocalizes
Glc7p from the nucleus and prevents chromosome
segregation; potential Cdc28p substrate"
/codon_start=1
/product="protein phosphatase regulator GIP4"
/protein_id="NP_009371.1"
/db_xref="GeneID:851202"
/db_xref="SGD:S000000029"
/translation="MVDVQKRKKLLAKAAASASIPAIKGSVPLDSYDIKIIQYKNALY
KLNELNRLLNVLVPHLKKKRDNDESYKIIPLVNFILSLCEGPIFNVSPVLAKRYHLLC
RFQLIKLSEVQQRLSTNFIDVEGWMFPEEVPLDHYKSCIYNNSLQWKILNSLSCIAQN
AIKIYNAKLRQILLERDAYKARSLPFDTSIIEDLLNPVEMTLILDLAVLINDPVRDKS
THSFYKLQWQVMEKLNSCVHSKIFPILRTYYNQLQKFSETRPTSLSNLQKDLPHWEWT
LHRIYTFHLRVFSVLCVIISFSRQIFLPNKQHFLDIKTRLSSENVYHYDLIICELMAL
LSPECDDVTALFELQENLKFWTQTARTDNNSSRTPIFHLQPGLVVELFNNHICKIIPK
LRSIMGLLSNWMDCWKYIEKNYKTFDETNDLRENLKEKLERDKALYLEVKNAKSKLKK
KPSITKLPASSSPSPSPTSSASPSRQASLESIRTRARAHLASNSSRSPSVSPVRTTFN
NKNAETKKSVVSPEKRKLINGRRPRSSSLQSYTNKQQTSYLNSTRHPSIAPPSKLNNQ
RSNSLQSSTMTLNQKIVQDTVRHLMNKSASTPNPSASSSLAPSPKVSSINNTSSGKSS
STLIANSSDTLAIETLTLDPESNSSELSIKRVRFAGVPPMTEAENPKPTKVGWYKKPA
VLHYPPIPASAMIKPLQHKSKYNTLRQEEGFTFRKSLRDGLEWENGESGSETTMMPFG
IEIKESTGHRIASKIRSKLR"
gene <87286..>87752
/gene="SNC1"
/locus_tag="YAL030W"
/db_xref="GeneID:851203"
mRNA join(<87286..87387,87501..>87752)
/gene="SNC1"
/locus_tag="YAL030W"
/product="SNAP receptor SNC1"
/transcript_id="NM_001178175.1"
/db_xref="GeneID:851203"
CDS join(87286..87387,87501..87752)
/gene="SNC1"
/locus_tag="YAL030W"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:14981247]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:16452633]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:36125415|PMID:16452633]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:36125415|PMID:10637288]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:20059749]"
/experiment="EXISTENCE:direct assay:GO:0006906 vesicle
fusion [PMID:11001046]"
/experiment="EXISTENCE:direct assay:GO:0030658 transport
vesicle membrane [PMID:10637288]"
/experiment="EXISTENCE:direct assay:GO:0031201 SNARE
complex [PMID:9195974]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0006887
exocytosis [PMID:9195971]"
/experiment="EXISTENCE:mutant phenotype:GO:0006893 Golgi
to plasma membrane transport [PMID:8374953]"
/experiment="EXISTENCE:mutant phenotype:GO:0006897
endocytosis [PMID:11029060]"
/note="Vesicle membrane receptor protein (v-SNARE);
involved in the fusion between Golgi-derived secretory
vesicles with the plasma membrane; proposed to be involved
in endocytosis; member of the synaptobrevin/VAMP family of
R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that
arose from the whole genome duplication"
/codon_start=1
/product="SNAP receptor SNC1"
/protein_id="NP_009372.1"
/db_xref="GeneID:851203"
/db_xref="SGD:S000000028"
/translation="MSSSTPFDPYALSEHDEERPQNVQSKSRTAELQAEIDDTVGIMR
DNINKVAERGERLTSIEDKADNLAVSAQGFKRGANRVRKAMWYKDLKMKMCLALVIII
LLVVIIVPIAVHFSR"
gene complement(<87855..>92270)
/gene="MYO4"
/locus_tag="YAL029C"
/gene_synonym="FUN22; SHE1"
/db_xref="GeneID:851204"
mRNA complement(<87855..>92270)
/gene="MYO4"
/locus_tag="YAL029C"
/gene_synonym="FUN22; SHE1"
/product="myosin 4"
/transcript_id="NM_001178174.1"
/db_xref="GeneID:851204"
CDS complement(87855..92270)
/gene="MYO4"
/locus_tag="YAL029C"
/gene_synonym="FUN22; SHE1"
/experiment="EXISTENCE:direct assay:GO:0000146
microfilament motor activity [PMID:11381095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:12725728|PMID:20439999]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:20439999]"
/experiment="EXISTENCE:direct assay:GO:0031941 filamentous
actin [PMID:11381095]"
/experiment="EXISTENCE:direct assay:GO:0051015 actin
filament binding [PMID:11381095]"
/experiment="EXISTENCE:mutant phenotype:GO:0007533 mating
type switching [PMID:8625407]"
/experiment="EXISTENCE:mutant phenotype:GO:0008298
intracellular mRNA localization
[PMID:9219698|PMID:9288973|PMID:11030653]"
/experiment="EXISTENCE:mutant phenotype:GO:0048309
endoplasmic reticulum inheritance
[PMID:14691136|PMID:16890529]"
/experiment="EXISTENCE:physical interaction:GO:0008298
intracellular mRNA localization
[PMID:10212145|PMID:11030653]"
/note="Type V myosin motor involved in actin-based
transport of cargos; required for mRNA transport,
including ASH1 mRNA, and facilitating the growth and
movement of ER tubules into the growing bud along with
She3p; MYO4 has a paralog, MYO2, that arose from the whole
genome duplication"
/codon_start=1
/product="myosin 4"
/protein_id="NP_009373.1"
/db_xref="GeneID:851204"
/db_xref="SGD:S000000027"
/translation="MSFEVGTKCWYPHKEQGWIGGEVTKNDFFEGTFHLELKLEDGET
VSIETNSFENDDDHPTLPVLRNPPILESTDDLTTLSYLNEPAVLHAIKKRYMNGQIYT
YSGIVLIAANPFDKVDHLYSREMIQNYSSKRKDELEPHLFAIAEEAYRFMVHEKANQT
VVVSGESGAGKTVSAKYIMRYFASVQESNNREGEVEMSQIESQILATNPIMEAFGNAK
TTRNDNSSRFGKYLQILFDENTTIRGSKIRTYLLEKSRLVYQPETERNYHIFYQILEG
LPEPVKQELHLSSPKDYHYTNQGGQPNIAGIDEAREYKITTDALSLVGINHETQLGIF
KILAGLLHIGNIEMKMTRNDASLSSEEQNLQIACELLGIDPFNFAKWIVKKQIVTRSE
KIVTNLNYNQALIARDSVAKFIYSTLFDWLVDNINKTLYDPELDQQDHVFSFIGILDI
YGFEHFEKNSFEQFCINYANEKLQQEFNQHVFKLEQEEYVKEEIEWSFIEFSDNQPCI
DLIENKLGILSLLDEESRLPSGSDESWASKLYSAFNKPPSNEVFSKPRFGQTKFIVSH
YAVDVEYEVEGFIEKNRDSVSLGHLDVFKATTNPIFKQILDNRELRSDDAPEEQNTEK
KIMIPARLSQKKPTLGSMFKKSLGELMAIINSTNVHYIRCIKPNSEKKPWEFDNLMVL
SQLRACGVLETIRISCAGFPSRWTFDEFVQRYFLLTDYSLWSGILYNPDLPKEAIVNF
CQSILDATISDSAKYQIGNTKIFFKAGMLAFLEKLRTNKMNEICIIIQKKIRARYYRL
QYLQTMESIKKCQSQIRSLLVRTRVDHELKTRAAILLQTNIRALWKREYYRAAIGQII
KLQCTCKRKLILDSVNRKFMLMAAVIIQSYIRSYGHKTDYRTLKRSSILVQSAMRMQL
ARRRYIVLQKEVEERNIRASYGIGLLEEAIEFKNSFILNLEMLNDSYTRLTQLLQGDL
SNIPSKQRQEYETIVNGYNDKISKLKTLQVEIMNTLNKKNALKERKKKQSSLIQSHMQ
SLAAIKGNKPSRLSDEVKSMKQELAFIENVIAQDFTTTYSANKNDKVKGLGIAGQQVK
PKLVNVIRRESGNPDLLELLMDLNCYTLEVTEGYLKKVNVTEVNGDNVLGPIHVITTV
VSSLVRNGLLIQSSKFISKVLLTVESIVMSLPKDETMLGGIFWLSNLSRLPAFAANQK
TLYEANGGDEKDKLTLIYLNDLENETLKVFDKIYSTWLVKFMKHASAHIEIFDMVLNE
KLFKNSGDEKFAKLFTFLNEFDAVLCKFQVVDSMHTKIFNDTLKYLNVMLFNDLITKC
PALNWKYGYEVDRNIERLVSWFEPRIEDVRPNLIQIIQAVKILQLKISNLNEFKLLFD
FWYALNPAQIQAILLKYKPANKGEAGVPNEILNYLANVIKRENLSLPGKMEIMLSAQF
DSAKNHLRYDTSAITQNSNTEGLATVSKIIKLDRK"
gene <92900..>94486
/gene="FRT2"
/locus_tag="YAL028W"
/gene_synonym="HPH2"
/db_xref="GeneID:851205"
mRNA <92900..>94486
/gene="FRT2"
/locus_tag="YAL028W"
/gene_synonym="HPH2"
/product="Frt2p"
/transcript_id="NM_001178173.1"
/db_xref="GeneID:851205"
CDS 92900..94486
/gene="FRT2"
/locus_tag="YAL028W"
/gene_synonym="HPH2"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:15189990]"
/experiment="EXISTENCE:mutant phenotype:GO:0071469
cellular response to alkaline pH [PMID:15189990]"
/experiment="EXISTENCE:mutant phenotype:GO:0071472
cellular response to salt stress [PMID:15189990]"
/experiment="EXISTENCE:physical interaction:GO:0031204
post-translational protein targeting to membrane,
translocation [PMID:21097665]"
/note="Tail-anchored ER membrane hypothetical protein;
interacts with homolog Frt1p; promotes growth in
conditions of high Na+, alkaline pH, or cell wall stress,
possibly via a role in posttranslational translocation;
potential Cdc28p substrate; FRT2 has a paralog, FRT1, that
arose from the whole genome duplication"
/codon_start=1
/product="Frt2p"
/protein_id="NP_009374.1"
/db_xref="GeneID:851205"
/db_xref="SGD:S000000026"
/translation="MQNAQIKSSSKGSGIDGTDRNSKDGVEKRPLEDVKQMIDAGTPD
VGHKSTVETKPNVGWQASHSNLAALHEKEQKYEMEHHHARHKLHRQVIPDYTSASTAM
FSDCMFNAAPDKVRSLSTMKSSGLSPKHPFNVVATFKGPFPQHSVESKPLDGGYSAKD
HFPSFKMLQAQQHPAHRHYKDNDKYGLKSPSRSFVKDKKRLVHRFLKSMEPSSSGQSK
DSSALAPAFDPILPNVISKPSKRPTHHSHSSDGSSSTQTDISLQSLLYHDLESSPKKH
VSPSRPPSVASESSPAVANPIGLSPKDACNASFSQSSSSSLSSSSSSSSSTSFSQSVA
VDPLEPPGNITYSSSNLSLNSDELDYYQRHIGLQLQQTEALLKHSLKDEVLKDENDLV
KNIANFDKIVKELRDLRSRTIGWKELVEEDYLMNLKQDFDKENPESFEARLSDTINTN
VAKLQDLEKRMASCKDRLASRKEVMRKMESLLSLENSLMISKKNVTFASKYRNEALDI
VFLIIIIVICYTFKHLVSHK"
gene <94687..>95472
/gene="SAW1"
/locus_tag="YAL027W"
/db_xref="GeneID:851206"
mRNA <94687..>95472
/gene="SAW1"
/locus_tag="YAL027W"
/product="DNA-binding protein SAW1"
/transcript_id="NM_001178172.1"
/db_xref="GeneID:851206"
CDS 94687..95472
/gene="SAW1"
/locus_tag="YAL027W"
/experiment="EXISTENCE:direct assay:GO:0000403 Y-form DNA
binding [PMID:23299942]"
/experiment="EXISTENCE:direct assay:GO:0000405 bubble DNA
binding [PMID:23299942]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0070337
3'-flap-structured DNA binding [PMID:23299942]"
/experiment="EXISTENCE:direct assay:GO:0070338
5'-flap-structured DNA binding [PMID:23299942]"
/experiment="EXISTENCE:genetic interaction:GO:0000736
double-strand break repair via single-strand annealing,
removal of nonhomologous ends [PMID:18471978]"
/experiment="EXISTENCE:mutant phenotype:GO:0000736
double-strand break repair via single-strand annealing,
removal of nonhomologous ends [PMID:18471978]"
/note="5'- and 3'-flap DNA binding protein; required for
recruitment of Rad1p-Rad10p to single-strand annealing
intermediates with 3' non-homologous tails for removal
during double-strand break repair; forms a complex with
Rad1p-Rad10p and stimulates its endonuclease activity;
green fluorescent protein (GFP)-fusion protein localizes
to the nucleus"
/codon_start=1
/product="DNA-binding protein SAW1"
/protein_id="NP_009375.1"
/db_xref="GeneID:851206"
/db_xref="SGD:S000000025"
/translation="MAPSIATVKIARDMVLPLRIFVNRKQILQTNDKTSNKSNATIFE
APLLSNNSIICLKSPNTRIYLSQQDKKNLCDEIKEDLLLIVYELASPEIISSVLSKIR
VGHSTDFQINVLPKLFAGADTDNAVTSHIQSVTRLAKFKYKLHYKHKWELDIFINSIK
KIANLRHYLMFQTLTLNGFSLNAGPKTLLARKIEKQPQVPNLLIENGDADALDTPVEE
DIKPVIEFMYKPVINLGEIIDVHVLHRPRRHKVRTQSKQPQEE"
gene complement(<95630..>99697)
/gene="DRS2"
/locus_tag="YAL026C"
/gene_synonym="FUN38; SWA3"
/db_xref="GeneID:851207"
mRNA complement(<95630..>99697)
/gene="DRS2"
/locus_tag="YAL026C"
/gene_synonym="FUN38; SWA3"
/product="aminophospholipid-translocating P4-type ATPase
DRS2"
/transcript_id="NM_001178171.2"
/db_xref="GeneID:851207"
CDS complement(95630..99697)
/gene="DRS2"
/locus_tag="YAL026C"
/gene_synonym="FUN38; SWA3"
/EC_number="7.6.2.1"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005802 trans-Golgi
network [PMID:12221123|PMID:15090616|PMID:10601336]"
/experiment="EXISTENCE:genetic interaction:GO:0006892
post-Golgi vesicle-mediated transport [PMID:10601336]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:12631737|PMID:16195350]"
/experiment="EXISTENCE:genetic interaction:GO:0090555
phosphatidylethanolamine flippase activity
[PMID:16452632]"
/experiment="EXISTENCE:genetic interaction:GO:0140345
phosphatidylcholine flippase activity [PMID:16452632]"
/experiment="EXISTENCE:genetic interaction:GO:0140346
phosphatidylserine flippase activity [PMID:16452632]"
/experiment="EXISTENCE:mutant phenotype:GO:0006892
post-Golgi vesicle-mediated transport [PMID:10601336]"
/experiment="EXISTENCE:mutant phenotype:GO:0032456
endocytic recycling [PMID:24272750]"
/experiment="EXISTENCE:mutant phenotype:GO:0045332
phospholipid translocation [PMID:15249668|PMID:16452632]"
/experiment="EXISTENCE:mutant phenotype:GO:0090555
phosphatidylethanolamine flippase activity
[PMID:16452632]"
/experiment="EXISTENCE:mutant phenotype:GO:0140346
phosphatidylserine flippase activity
[PMID:16452632|PMID:8633245|PMID:15249668]"
/experiment="EXISTENCE:physical interaction:GO:1990530
Cdc50p-Drs2p complex [PMID:15090616]"
/note="Trans-golgi network aminophospholipid translocase
(flippase); P4-type ATPase; maintains membrane lipid
asymmetry in post-Golgi secretory vesicles; contributes to
clathrin-coated vesicle formation, endocytosis, protein
trafficking between the Golgi and endosomal system and the
cellular response to mating pheromone; autoinhibited by
its C-terminal tail; localizes to the trans-Golgi network"
/codon_start=1
/product="aminophospholipid-translocating P4-type ATPase
DRS2"
/protein_id="NP_009376.2"
/db_xref="GeneID:851207"
/db_xref="SGD:S000000024"
/translation="MNDDRETPPKRKPGEDDTLFDIDFLDDTTSHSGSRSKVTNSHAN
ANYIPPSHVLPEETIDLDADDDNIENDVHENLFMSNNHDDQTSWNANRFDSDAYQPQS
LRAVKPPGLFARFGNGLKNAFTFKRKKGPESFEMNHYNAVTNNELDDNYLDSRNKFNI
KILFNRYILRKNVGDAEGNGEPRVIHINDSLANSSFGYSDNHISTTKYNFATFLPKFL
FQEFSKYANLFFLCTSAIQQVPHVSPTNRYTTIGTLLVVLIVSAMKECIEDIKRANSD
KELNNSTAEIFSEAHDDFVEKRWIDIRVGDIIRVKSEEPIPADTIILSSSEPEGLCYI
ETANLDGETNLKIKQSRVETAKFIDVKTLKNMNGKVVSEQPNSSLYTYEGTMTLNDRQ
IPLSPDQMILRGATLRNTAWIFGLVIFTGHETKLLRNATATPIKRTAVEKIINRQIIA
LFTVLIVLILISSIGNVIMSTADAKHLSYLYLEGTNKAGLFFKDFLTFWILFSNLVPI
SLFVTVELIKYYQAFMIGSDLDLYYEKTDTPTVVRTSSLVEELGQIEYIFSDKTGTLT
RNIMEFKSCSIAGHCYIDKIPEDKTATVEDGIEVGYRKFDDLKKKLNDPSDEDSPIIN
DFLTLLATCHTVIPEFQSDGSIKYQAASPDEGALVQGGADLGYKFIIRKPNSVTVLLE
ETGEEKEYQLLNICEFNSTRKRMSAIFRFPDGSIKLFCKGADTVILERLDDEANQYVE
ATMRHLEDYASEGLRTLCLAMRDISEGEYEEWNSIYNEAATTLDNRAEKLDEAANLIE
KNLILIGATAIEDKLQDGVPETIHTLQEAGIKIWVLTGDRQETAINIGMSCRLLSEDM
NLLIINEETRDDTERNLLEKINALNEHQLSTHDMNTLALVIDGKSLGFALEPELEDYL
LTVAKLCKAVICCRVSPLQKALVVKMVKRKSSSLLLAIGDGANDVSMIQAAHVGVGIS
GMEGMQAARSADIAVGQFKFLKKLLLVHGSWSYQRISVAILYSFYKNTALYMTQFWYV
FANAFSGQSIMESWTMSFYNLFFTVWPPFVIGVFDQFVSSRLLERYPQLYKLGQKGQF
FSVYIFWGWIINGFFHSAIVFIGTILIYRYGFALNMHGELADHWSWGVTVYTTSVIIV
LGKAALVTNQWTKFTLIAIPGSLLFWLIFFPIYASIFPHANISREYYGVVKHTYGSGV
FWLTLIVLPIFALVRDFLWKYYKRMYEPETYHVIQEMQKYNISDSRPHVQQFQNAIRK
VRQVQRMKKQRGFAFSQAEEGGQEKIVRMYDTTQKRGKYGELQDASANPFNDNNGLGS
NDFESAEPFIENPFADGNQNSNRFSSSRDDISFDI"
gene 99305..99868
/gene="HRA1"
/locus_tag="YNCA0001W"
/db_xref="GeneID:9164866"
ncRNA 99305..99868
/ncRNA_class="other"
/gene="HRA1"
/locus_tag="YNCA0001W"
/product="HRA1"
/note="Non-protein-coding RNA; substrate of RNase P,
possibly involved in rRNA processing, specifically
maturation of 20S precursor into the mature 18S rRNA"
/transcript_id="NR_132149.1"
/db_xref="GeneID:9164866"
/db_xref="SGD:S000119380"
gene complement(<100225..>101145)
/gene="MAK16"
/locus_tag="YAL025C"
/db_xref="GeneID:851208"
mRNA complement(<100225..>101145)
/gene="MAK16"
/locus_tag="YAL025C"
/product="ribosome biosynthesis protein MAK16"
/transcript_id="NM_001178170.2"
/db_xref="GeneID:851208"
CDS complement(100225..101145)
/gene="MAK16"
/locus_tag="YAL025C"
/experiment="EXISTENCE:direct assay:GO:0005730 nucleolus
[PMID:10838225]"
/experiment="EXISTENCE:direct assay:GO:0030687
preribosome, large subunit precursor [PMID:11583614]"
/experiment="EXISTENCE:mutant phenotype:GO:0000463
maturation of LSU-rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16710831]"
/experiment="EXISTENCE:mutant phenotype:GO:0000466
maturation of 5.8S rRNA from tricistronic rRNA transcript
(SSU-rRNA, 5.8S rRNA, LSU-rRNA) [PMID:16710831]"
/experiment="EXISTENCE:mutant phenotype:GO:0042273
ribosomal large subunit biogenesis [PMID:7739558]"
/note="Essential nuclear protein; constituent of 66S
pre-ribosomal particles; required for maturation of 25S
and 5.8S rRNAs; required for maintenance of M1 satellite
double-stranded RNA of the L-A virus; contains an Fe/S
cluster that functions as a redox sensor and is required
for Mak16p-Rpf1p complex formation and ribosome
biogenesis"
/codon_start=1
/product="ribosome biosynthesis protein MAK16"
/protein_id="NP_009377.2"
/db_xref="GeneID:851208"
/db_xref="SGD:S000000023"
/translation="MSDEIVWQVINQSFCSHRIKAPNGQNFCRNEYNVTGLCTRQSCP
LANSKYATVKCDNGKLYLYMKTPERAHTPAKLWERIKLSKNYTKALQQIDEHLLHWSK
FFRHKCKQRFTKLTQVMITERRLALREEERHYVGVAPKVKRREQNRERKALVAAKIEK
AIEKELMDRLKSGAYGDKPLNVDEKVWKKIMGQMEEENSQDEEEDWDEEEESDDGEVE
YVADDGEGEYVDVDDLEKWLADSDREASSASESESDSESESDSDSDEENKNSAKRRKK
GTSAKTKRPKVEIEYEEEHEVQNAEQEVAQ"
gene complement(<101565..>105872)
/gene="LTE1"
/locus_tag="YAL024C"
/gene_synonym="MSI2"
/db_xref="GeneID:851209"
mRNA complement(<101565..>105872)
/gene="LTE1"
/locus_tag="YAL024C"
/gene_synonym="MSI2"
/product="mitotic regulator LTE1"
/transcript_id="NM_001178169.1"
/db_xref="GeneID:851209"
CDS complement(101565..105872)
/gene="LTE1"
/locus_tag="YAL024C"
/gene_synonym="MSI2"
/experiment="EXISTENCE:direct assay:GO:0005933 cellular
bud [PMID:12782684]"
/experiment="EXISTENCE:direct assay:GO:1902480 protein
localization to mitotic spindle [PMID:19948498]"
/experiment="EXISTENCE:genetic interaction:GO:0016192
vesicle-mediated transport [PMID:17121813]"
/experiment="EXISTENCE:mutant phenotype:GO:0031536
positive regulation of exit from mitosis [PMID:27798238]"
/experiment="EXISTENCE:mutant phenotype:GO:0031578 mitotic
spindle orientation checkpoint signaling [PMID:19948498]"
/note="Protein similar to GDP/GTP exchange factors;
without detectable GEF activity; required for asymmetric
localization of Bfa1p at daughter-directed spindle pole
bodies and for mitotic exit at low temperatures"
/codon_start=1
/product="mitotic regulator LTE1"
/protein_id="NP_009378.1"
/db_xref="GeneID:851209"
/db_xref="SGD:S000000022"
/translation="MEIFSQKDYYPTPSSNVISYESDCVSKPVNSADLPALIVHLSSP
LEGVDYNASADFFLIYRNFITPQDLHDLLIYRFRWCIREITTNAAKAKRRRIGEVALV
RTFVLLRHSILNYFVQDFLPNITLRLRLIEFLNDKHIEQYPKIISSCIINLKKNWVHC
SKLVWENIELNEPDKLDFDAWLHYSLKDFTQLESLHKRGSRLSIYARQSFASPDFRNQ
SVLSLYKTSDVFRLPEKLQSSNSSKNQRSPSMLLFPDNTSNVYSKHRIAKEPSVDNES
EDMSDSKQKISHLSKVTLVSTLMKGVDYPSSYAVDKIMPPTPAKKVEFILNSLYIPED
LNEQSGTLQGTSTTSSLDNNSNSNSRSNTSSMSVLHRSAIGLLAKWMKNHNRHDSSND
KKFMSAIKPANQKPEMDAFVKYVVSISSLNRKSSKEEEEEFLNSDSSKFDILSARTID
EVESLLHLQNQLIEKVQTHSNNNRGPTVNVDCERREHIHDIKILQQNSFKPSNDNFSA
MDNLDLYQTVSSIAQSVISLTNTLNKQLQNNESNMQPSPSYDALQRRKVKSLTTAYYN
KMHGSYSAESMRLFDKDNSSSRTDENGPQRLLFHETDKTNSEAITNMTPRRKNHSQSQ
KSMTSSPLKNVLPDLKESSPLNDSREDTESITYSYDSELSSSSPPRDTVTKKSRKVRN
IVNNTDSPTLKTKTGFLNLREFTFEDTKSLDEKKSTIDGLEKNYDNKENQESEYESTK
KLDNSLDASSEANNYDITTRKKHSSCNHKIKQAVVRPASGRISISRVQSIAITPTKEL
SIVDPEQNKSNSVIEEISEIEPLNLEYNKKSALYSDTSSTVISISTSKLFESAQNSPL
KQTQNPQREFPNGTSVSETNRIRLSIAPTIESVVSDLNSITTGSTVETFETSRDLPVP
HQRIINLREEYQRGNQDIISNTSSLHELKTIDLSDSNNDLESPSTHAKNNKYFFSPDD
GSIDVASPMKNVEELKSKFLKNESETNSNISGSVLTMDDIDINDTSSARNTRRANSES
AFTGSLNKKNLNEIANMLDDSINDDPITVALMKLEGTYEKIPEKPENTKSSDAIGIKT
SKLADEVEMLNLNNLPSFQNSPAEKRKSLLIERRRQTIMNIPFTPDQSEKEGFTSSSP
EKIDVSANVDVAVQAAQIQELIGQYRIHDSRLMISNNESHVPFILMYDSLSVAQQMTL
IEKEILGEIDWKDLLDLKMKHEGPQVISWLQLLVRNETLSGIDLAISRFNLTVDWIIS
EILLTKSSKMKRNVIQRFIHVADHCRTFQNFNTLMEIILALSSSVVQKFTDAWRLIEP
GDLLTWEELKKIPSLDRNYSTIRNLLNSVNPLVGCVPFIVVYLSDLSANAEKKDWILE
DKVVNYNKFDTNVQIVKNFIQRVQWSKFYTFKVNHELLSKCVYISTLTQEEINELST"
gene complement(<106272..>108551)
/gene="PMT2"
/locus_tag="YAL023C"
/gene_synonym="FUN25"
/db_xref="GeneID:851210"
mRNA complement(<106272..>108551)
/gene="PMT2"
/locus_tag="YAL023C"
/gene_synonym="FUN25"
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase PMT2"
/transcript_id="NM_001178168.1"
/db_xref="GeneID:851210"
CDS complement(106272..108551)
/gene="PMT2"
/locus_tag="YAL023C"
/gene_synonym="FUN25"
/EC_number="2.4.1.109"
/experiment="EXISTENCE:direct assay:GO:0004169
dolichyl-phosphate-mannose-protein mannosyltransferase
activity [PMID:21956107]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0097582
dolichyl-phosphate-mannose-protein mannosyltransferase
Pmt1p-Pmt2p dimer complex [PMID:12551906]"
/experiment="EXISTENCE:direct assay:GO:0097584
dolichyl-phosphate-mannose-protein mannosyltransferase
Pmt5p-Pmt2p dimer complex [PMID:12551906]"
/experiment="EXISTENCE:genetic interaction:GO:0004169
dolichyl-phosphate-mannose-protein mannosyltransferase
activity [PMID:21956107|PMID:8543034|PMID:7852348]"
/experiment="EXISTENCE:genetic interaction:GO:0032527
protein exit from endoplasmic reticulum [PMID:21147851]"
/experiment="EXISTENCE:genetic interaction:GO:0035269
protein O-linked glycosylation via mannose
[PMID:21231968|PMID:7852348|PMID:8543034]"
/experiment="EXISTENCE:genetic interaction:GO:0036503 ERAD
pathway [PMID:21147851]"
/experiment="EXISTENCE:genetic interaction:GO:1900101
regulation of endoplasmic reticulum unfolded protein
response [PMID:21231968|PMID:23704572]"
/experiment="EXISTENCE:mutant phenotype:GO:0004169
dolichyl-phosphate-mannose-protein mannosyltransferase
activity [PMID:7852348|PMID:8543034]"
/experiment="EXISTENCE:mutant phenotype:GO:0006493 protein
O-linked glycosylation [PMID:9184828]"
/experiment="EXISTENCE:mutant phenotype:GO:0035269 protein
O-linked glycosylation via mannose
[PMID:18182384|PMID:7852348|PMID:8543034]"
/experiment="EXISTENCE:mutant phenotype:GO:0036503 ERAD
pathway [PMID:18182384]"
/experiment="EXISTENCE:mutant phenotype:GO:1900101
regulation of endoplasmic reticulum unfolded protein
response [PMID:23704572]"
/experiment="EXISTENCE:physical interaction:GO:0097582
dolichyl-phosphate-mannose-protein mannosyltransferase
Pmt1p-Pmt2p dimer complex [PMID:8543034|PMID:21956107]"
/note="Protein O-mannosyltransferase of the ER membrane;
transfers mannose residues from dolichyl
phosphate-D-mannose to protein serine/threonine residues;
involved in ER quality control; functions as a heterodimer
with Pmt2p but can also pair with Pmt5p; antifungal drug
target; PMT2 has a paralog, PMT3, that arose from the
whole genome duplication"
/codon_start=1
/product="dolichyl-phosphate-mannose-protein
mannosyltransferase PMT2"
/protein_id="NP_009379.2"
/db_xref="GeneID:851210"
/db_xref="SGD:S000000021"
/translation="MSSSSSTGYSKNNAAHIKQENTLRQRESSSISVSEELSSADERD
AEDFSKEKPAAQSSLLRLESVVMPVIFTALALFTRMYKIGINNHVVWDEAHFGKFGSY
YLRHEFYHDVHPPLGKMLVGLSGYLAGYNGSWDFPSGEIYPDYLDYVKMRLFNASFSA
LCVPLAYFTAKAIGFSLPTVWLMTVLVLFENSYSTLGRFILLDSMLLFFTVASFFSFV
MFHNQRSKPFSRKWWKWLLITGISLGCTISVKMVGLFIITMVGIYTVIDLWTFLADKS
MSWKTYINHWLARIFGLIIVPFCIFLLCFKIHFDLLSHSGTGDANMPSLFQARLVGSD
VGQGPRDIALGSSVVSIKNQALGGSLLHSHIQTYPDGSNQQQVTCYGYKDANNEWFFN
RERGLPSWSENETDIEYLKPGTSYRLVHKSTGRNLHTHPVAAPVSKTQWEVSGYGDNV
VGDNKDNWVIEIMDQRGDEDPEKLHTLTTSFRIKNLEMGCYLAQTGNSLPEWGFRQQE
VVCMKNPFKRDKRTWWNIETHENERLPPRPEDFQYPKTNFLKDFIHLNLAMMATNNAL
VPDPDKFDYLASSAWQWPTLNVGLRLCGWGDDNPKYFLLGTPASTWASSVAVLAFMAT
VVILLIRWQRQYVDLRNPSNWNVFLMGGFYPLLAWGLHYMPFVIMSRVTYVHHYLPAL
YFALIILAYCFDAGLQKWSRSKCGRIMRFVLYAGFMALVIGCFWYFSPISFGMEGPSS
NFRYLNWFSTWDIADKQEA"
gene complement(<108877..>110430)
/gene="FUN26"
/locus_tag="YAL022C"
/db_xref="GeneID:851211"
mRNA complement(<108877..>110430)
/gene="FUN26"
/locus_tag="YAL022C"
/product="nucleoside transmembrane transporter FUN26"
/transcript_id="NM_001178167.1"
/db_xref="GeneID:851211"
CDS complement(108877..110430)
/gene="FUN26"
/locus_tag="YAL022C"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:21349851|PMID:26928762|PMID:19001347]"
/experiment="EXISTENCE:direct assay:GO:0005337 nucleoside
transmembrane transporter activity
[PMID:10827169|PMID:25035431]"
/experiment="EXISTENCE:direct assay:GO:0015205 nucleobase
transmembrane transporter activity [PMID:25035431]"
/experiment="EXISTENCE:direct assay:GO:0015851 nucleobase
transport [PMID:25035431]"
/experiment="EXISTENCE:direct assay:GO:0015858 nucleoside
transport [PMID:25035431|PMID:10827169]"
/experiment="EXISTENCE:direct assay:GO:0055085
transmembrane transport [PMID:10827169]"
/experiment="EXISTENCE:mutant phenotype:GO:0034257
nicotinamide riboside transmembrane transporter activity
[PMID:21349851]"
/experiment="EXISTENCE:mutant phenotype:GO:0034258
nicotinamide riboside transport [PMID:21349851]"
/note="High affinity, broad selectivity,
nucleoside/nucleobase transporter; vacuolar membrane
localized transporter which may regulate the balance of
nicotinamide riboside (NmR) levels between the cytosol and
vacuole, contributing to salvage of NmR for use in
cytosolic NAD+ synthesis; equilibrative nucleoside
transporter (ENT) family member"
/codon_start=1
/product="nucleoside transmembrane transporter FUN26"
/protein_id="NP_009380.1"
/db_xref="GeneID:851211"
/db_xref="SGD:S000000020"
/translation="MSTSADTDTIKKPILAVPEPALADTHSEEISRSGEEHESENNEH
SDEEGDNYSEREQSVSTEPLDTLPLRKKLKNLSYITFFAIGIGLLWPWNCILSASQYF
KHDIFKDTSIWAKIFTSSMMSFSTISSMLFNIYLAKRQYKYSRRVINGLVWEIIVFTV
MCFFTILHFLLPKWFNFMFIMMLVVISSMGTAMTQNGIMAIANVFGSEYSQGVMVGQA
VAGVLPSLVLFALAFIENSSVSTTGGILLYFFTTTLVVTICVVMFSVSKISRKVNENW
NVEDGHITDVLLGSLRSNEEEIRIVGRIDQMEDEDHRRTNGTRDDNDEGEELQLKVPF
EVLFAKLKYLVLSIFTTFVVTLVFPVFASATYVTGLPLSNAQYIPLIFTLWNLGDLYG
RVIADWPMFRDQKFTPRKTFIYSLLRVAAIPLFLMFTAITSSSSGDEEHNGSVIVDLC
YMLLQFLFGVTNGHVISMSFMKVPEQLDNDDEKEAAGGFTNIFVSTGLALGSIISYVF
VFIIDFIIR"
gene complement(<110846..>113359)
/gene="CCR4"
/locus_tag="YAL021C"
/gene_synonym="FUN27; NUT21"
/db_xref="GeneID:851212"
mRNA complement(<110846..>113359)
/gene="CCR4"
/locus_tag="YAL021C"
/gene_synonym="FUN27; NUT21"
/product="CCR4-NOT core exoribonuclease subunit CCR4"
/transcript_id="NM_001178166.1"
/db_xref="GeneID:851212"
CDS complement(110846..113359)
/gene="CCR4"
/locus_tag="YAL021C"
/gene_synonym="FUN27; NUT21"
/EC_number="3.1.13.4"
/experiment="EXISTENCE:direct assay:GO:0000175 3'-5'-RNA
exonuclease activity [PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0000932 P-body
[PMID:18611963]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11889048]"
/experiment="EXISTENCE:direct assay:GO:0030015 CCR4-NOT
core complex [PMID:10490603]"
/experiment="EXISTENCE:direct assay:GO:0032968 positive
regulation of transcription elongation by RNA polymerase
II [PMID:21406554]"
/experiment="EXISTENCE:genetic interaction:GO:0000076 DNA
replication checkpoint signaling [PMID:17158920]"
/experiment="EXISTENCE:genetic interaction:GO:0000175
3'-5'-RNA exonuclease activity [PMID:11889048]"
/experiment="EXISTENCE:genetic interaction:GO:0006260 DNA
replication [PMID:17158920]"
/experiment="EXISTENCE:genetic interaction:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11404327]"
/experiment="EXISTENCE:mutant phenotype:GO:0000175
3'-5'-RNA exonuclease activity
[PMID:11889048|PMID:11239395]"
/experiment="EXISTENCE:mutant phenotype:GO:0000288
nuclear-transcribed mRNA catabolic process,
deadenylation-dependent decay [PMID:11889048]"
/experiment="EXISTENCE:mutant phenotype:GO:0000289
nuclear-transcribed mRNA poly(A) tail shortening
[PMID:17158920|PMID:11410650]"
/experiment="EXISTENCE:mutant phenotype:GO:0006368
transcription elongation by RNA polymerase II
[PMID:11404327]"
/experiment="EXISTENCE:mutant phenotype:GO:0007089
traversing start control point of mitotic cell cycle
[PMID:15075273]"
/experiment="EXISTENCE:physical interaction:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:10490603]"
/experiment="EXISTENCE:physical interaction:GO:0016593
Cdc73/Paf1 complex [PMID:9891041]"
/note="Component of the CCR4-NOT transcriptional complex;
CCR4-NOT is involved in regulation of gene expression;
component of the major cytoplasmic deadenylase, which is
involved in mRNA poly(A) tail shortening"
/codon_start=1
/product="CCR4-NOT core exoribonuclease subunit CCR4"
/protein_id="NP_009381.1"
/db_xref="GeneID:851212"
/db_xref="SGD:S000000019"
/translation="MNDPSLLGYPNVGPQQQQQQQQQQHAGLLGKGTPNALQQQLHMN
QLTGIPPPGLMNNSDVHTSSNNNSRQLLDQLANGNANMLNMNMDNNNNNNNNNNNNNN
NGGGSGVMMNASTAAVNSIGMVPTVGTPVNINVNASNPLLHPHLDDPSLLNNPIWKLQ
LHLAAVSAQSLGQPNIYARQNAMKKYLATQQAQQAQQQAQQQAQQQVPGPFGPGPQAA
PPALQPTDFQQSHIAEASKSLVDCTKQALMEMADTLTDSKTAKKQQPTGDSTPSGTAT
NSAVSTPLTPKIELFANGKDEANQALLQHKKLSQYSIDEDDDIENRMVMPKDSKYDDQ
LWHALDLSNLQIFNISANIFKYDFLTRLYLNGNSLTELPAEIKNLSNLRVLDLSHNRL
TSLPAELGSCFQLKYFYFFDNMVTTLPWEFGNLCNLQFLGVEGNPLEKQFLKILTEKS
VTGLIFYLRDNRPEIPLPHERRFIEINTDGEPQREYDSLQQSTEHLATDLAKRTFTVL
SYNTLCQHYATPKMYRYTPSWALSWDYRRNKLKEQILSYDSDLLCLQEVESKTFEEYW
VPLLDKHGYTGIFHAKARAKTMHSKDSKKVDGCCIFFKRDQFKLITKDAMDFSGAWMK
HKKFQRTEDYLNRAMNKDNVALFLKLQHIPSGDTIWAVTTHLHWDPKFNDVKTFQVGV
LLDHLETLLKEETSHNFRQDIKKFPVLICGDFNSYINSAVYELINTGRVQIHQEGNGR
DFGYMSEKNFSHNLALKSSYNCIGELPFTNFTPSFTDVIDYIWFSTHALRVRGLLGEV
DPEYVSKFIGFPNDKFPSDHIPLLARFEFMKTNTGSKKV"
gene complement(<113614..>114615)
/gene="ATS1"
/locus_tag="YAL020C"
/gene_synonym="DPH8; FUN28; KTI13"
/db_xref="GeneID:851213"
mRNA complement(<113614..>114615)
/gene="ATS1"
/locus_tag="YAL020C"
/gene_synonym="DPH8; FUN28; KTI13"
/product="Ats1p"
/transcript_id="NM_001178165.2"
/db_xref="GeneID:851213"
CDS complement(113614..114615)
/gene="ATS1"
/locus_tag="YAL020C"
/gene_synonym="DPH8; FUN28; KTI13"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:18466297]"
/experiment="EXISTENCE:mutant phenotype:GO:0002098 tRNA
wobble uridine modification [PMID:18755837]"
/experiment="EXISTENCE:mutant phenotype:GO:0017183 protein
histidyl modification to diphthamide [PMID:37662670]"
/note="Protein required for modification of wobble
nucleosides in tRNA; acts with Elongator complex, Kti11p,
and Kti12p; has a potential role in regulatory
interactions between microtubules and the cell cycle;
forms a stable heterodimer with Kti11p"
/codon_start=1
/product="Ats1p"
/protein_id="NP_009382.2"
/db_xref="GeneID:851213"
/db_xref="SGD:S000000018"
/translation="MSCVYAFGSNGQRQLGLGHDEDMDTPQRSVPGDDGAIVRKIACG
GNHSVMLTNDGNLVGCGDNRRGELDSAQALRQVHDWRPVEVPAPVVDVACGWDTTVIV
DADGRVWQRGGGCYEFTQQHVPLNSNDERIAVYGCFQNFVVVQGTRVYGWGSNTKCQL
QEPKSRSLKEPVLVYDTGSVAVDYVAMGKDFMVIVDEGGRIVHASGRLPTGFELKQQQ
KRHNLVVLCMWTSIHLWNARLNTVESFGRGTHSQLFPQERLDFPIVGVATGSEHGILT
TANQEGKSHCYNVYCWGWGEHGNCGPQKGSQPGLQLVGQYSGKPRVFGGCATTWIVL"
gene <114919..>118314
/gene="FUN30"
/locus_tag="YAL019W"
/db_xref="GeneID:851214"
mRNA <114919..>118314
/gene="FUN30"
/locus_tag="YAL019W"
/product="DNA-dependent ATPase FUN30"
/transcript_id="NM_001178164.1"
/db_xref="GeneID:851214"
CDS 114919..118314
/gene="FUN30"
/locus_tag="YAL019W"
/EC_number="3.6.4.12"
/experiment="EXISTENCE:direct assay:GO:0000775 chromosome,
centromeric region [PMID:23028372]"
/experiment="EXISTENCE:direct assay:GO:0000785 chromatin
[PMID:39613969]"
/experiment="EXISTENCE:direct assay:GO:0003677 DNA binding
[PMID:20075079]"
/experiment="EXISTENCE:direct assay:GO:0003682 chromatin
binding [PMID:20075079]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922|PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0031934 mating-type
region heterochromatin [PMID:19956593]"
/experiment="EXISTENCE:direct assay:GO:0140658
ATP-dependent chromatin remodeler activity
[PMID:20075079|PMID:23779104]"
/experiment="EXISTENCE:genetic interaction:GO:0000706
meiotic DNA double-strand break processing
[PMID:39613969]"
/experiment="EXISTENCE:genetic interaction:GO:1990918
double-strand break repair involved in meiotic
recombination [PMID:39613969]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:23779104]"
/experiment="EXISTENCE:mutant phenotype:GO:0000183 rDNA
heterochromatin formation [PMID:19956593]"
/experiment="EXISTENCE:mutant phenotype:GO:0000706 meiotic
DNA double-strand break processing [PMID:39613969]"
/experiment="EXISTENCE:mutant phenotype:GO:0000729 DNA
double-strand break processing
[PMID:22960743|PMID:22960744]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling
[PMID:33141948|PMID:20075079|PMID:23779104]"
/experiment="EXISTENCE:mutant phenotype:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:19956593]"
/experiment="EXISTENCE:mutant phenotype:GO:0031507
heterochromatin formation [PMID:21388962]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation [PMID:19956593]"
/experiment="EXISTENCE:mutant phenotype:GO:0033120
positive regulation of RNA splicing [PMID:32234239]"
/experiment="EXISTENCE:mutant phenotype:GO:0140658
ATP-dependent chromatin remodeler activity
[PMID:39613969|PMID:19956593]"
/experiment="EXISTENCE:mutant phenotype:GO:1990918
double-strand break repair involved in meiotic
recombination [PMID:39613969]"
/note="SWI/SNF-like ATP-dependent chromatin remodeler;
involved in silencing at the MAT locus, telomeres and
centromeres; enriched at centromeres and required to
promote correct chromatin structure around centromeres and
at the boundary element of silent mating type loci;
recruited to DNA double-strand breaks (DSBs) where it
promotes 5' strand resection; role in meiotic DSB
resection and recombination; potential Cdc28p substrate"
/codon_start=1
/product="DNA-dependent ATPase FUN30"
/protein_id="NP_009383.1"
/db_xref="GeneID:851214"
/db_xref="SGD:S000000017"
/translation="MSGSHSNDEDDVVQVPETSSPTKVASSSPLKPTSPTVPDASVAS
LRSRFTFKPSDPSEGAHTSKPLPSGSPEVALVNLAREFPDFSQTLVQAVFKSNSFNLQ
SARERLTRLRQQRQNWTWNKNASPKKSETPPPVKKSLPLANTGRLSSIHGNINNKSSK
ITVAKQKTSIFDRYSNVINQKQYTFELPTNLNIDSEALSKLPVNYNKKRRLVRADQHP
IGKSYESSATQLGSAREKLLANRKYGRHANDNDEEEEESMMTDDDDASGDDYTESTPQ
INLDEQVLQFINDSDIVDLSDLSDTTMHKAQLIASHRPYSSLNAFVNTNFNDKDTEEN
ASNKRKRRAAASANESERLLDKITQSIRGYNAIESVIKKCSSYGDLVTSQMKKWGVQV
EGDNSELDLMNLGEDDDDDNDDGNNDNNNSNNNNTAGADATSKEKEDTKAVVEGFDET
SAEPTPAPAPAPVERETKRIRNTTKPKVVEDEDDDVDLEAIDDELPQSEHEDDDYEEE
DEDYNDEEEDVEYDDGDDDDDDDDEFVATRKNTHVISTTSRNGRKPIVKFFKGKPRLL
SPEISLKDYQQTGINWLNLLYQNKMSCILADDMGLGKTCQVISFFAYLKQINEPGPHL
VVVPSSTLENWLREFQKFAPALKIEPYYGSLQEREELRDILERNAGKYDVIVTTYNLA
AGNKYDVSFLKNRNFNVVVYDEGHMLKNSTSERFAKLMKIRANFRLLLTGTPLQNNLK
ELMSLLEFIMPNLFISKKESFDAIFKQRAKTTDDNKNHNPLLAQEAITRAKTMMKPFI
LRRRKDQVLKHLPPKHTHIQYCELNAIQKKIYDKEIQIVLEHKRMIKDGELPKDAKEK
SKLQSSSSKNLIMALRKASLHPLLFRNIYNDKIITKMSDAILDEPAYAENGNKEYIKE
DMSYMTDFELHKLCCNFPNTLSKYQLHNDEWMQSGKIDALKKLLKTIIVDKQEKVLIF
SLFTQVLDILEMVLSTLDYKFLRLDGSTQVNDRQLLIDKFYEDKDIPIFILSTKAGGF
GINLVCANNVIIFDQSFNPHDDRQAADRAHRVGQTKEVNITTLITKDSIEEKIHQLAK
NKLALDSYISEDKKSQDVLESKVSDMLEDIIYDENSKPKGTKE"
gene complement(<118564..>119541)
/gene="LDS1"
/locus_tag="YAL018C"
/db_xref="GeneID:851215"
mRNA complement(<118564..>119541)
/gene="LDS1"
/locus_tag="YAL018C"
/product="Lds1p"
/transcript_id="NM_001178163.1"
/db_xref="GeneID:851215"
CDS complement(118564..119541)
/gene="LDS1"
/locus_tag="YAL018C"
/experiment="EXISTENCE:direct assay:GO:0005619 ascospore
wall [PMID:23966878]"
/experiment="EXISTENCE:direct assay:GO:0005628 prospore
membrane [PMID:23966878]"
/experiment="EXISTENCE:direct assay:GO:0005811 lipid
droplet [PMID:24390141]"
/experiment="EXISTENCE:genetic interaction:GO:0030476
ascospore wall assembly [PMID:23966878]"
/note="Protein Involved in spore wall assembly; localizes
to lipid droplets found on or outside of the prospore
membrane; shares similarity with Lds2p and Rrt8p, and a
strain mutant for all 3 genes exhibits reduced dityrosine
fluorescence relative to the single mutants"
/codon_start=1
/product="Lds1p"
/protein_id="NP_009384.1"
/db_xref="GeneID:851215"
/db_xref="SGD:S000000016"
/translation="MSFTGSLALAGIGGLVYKFGGGQSYEKLPYVNIPFNQYLDKVYK
KHFSKVMSRTRYVLMNFFKDAFTGGAFMYPFKGFLEFNTNKSSYSTTMLGILSSYLIM
FALVSFVYWATITPMYTAFLIVLGPIGLFIAIFHSFLQANVFTLLFMRLSHFNNHLVE
VCLEKNGLEENLSEVKPIKYYAPINSIYFWAYYFPFKLVKYMLGLSVLFVLLVISFFP
LIGPILFHILISPFITQIYFTKVLRLQNFDNIQRRENIYLHAGQYASFGFLAGLIESV
PILAGFAISTNTIGSVLFNLDHPMVPENLVETQAEIEAAPQDINQQPNQ"
gene <120225..>124295
/gene="PSK1"
/locus_tag="YAL017W"
/gene_synonym="FUN31"
/db_xref="GeneID:851216"
mRNA <120225..>124295
/gene="PSK1"
/locus_tag="YAL017W"
/gene_synonym="FUN31"
/product="serine/threonine protein kinase PSK1"
/transcript_id="NM_001178162.2"
/db_xref="GeneID:851216"
CDS 120225..124295
/gene="PSK1"
/locus_tag="YAL017W"
/gene_synonym="FUN31"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22289182|PMID:14562095]"
/experiment="EXISTENCE:genetic interaction:GO:0060917
regulation of (1->6)-beta-D-glucan biosynthetic process
[PMID:17531808]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:12372297]"
/experiment="EXISTENCE:mutant phenotype:GO:0045719
negative regulation of glycogen biosynthetic process
[PMID:12372297]"
/note="PAS domain-containing serine/threonine protein
kinase; coordinately regulates protein synthesis and
carbohydrate metabolism and storage in response to a
unknown metabolite that reflects nutritional status; PSK1
has a paralog, PSK2, that arose from the whole genome
duplication"
/codon_start=1
/product="serine/threonine protein kinase PSK1"
/protein_id="NP_009385.2"
/db_xref="GeneID:851216"
/db_xref="SGD:S000000015"
/translation="MPYIGASNLSEHSFVNLKEKHAITHKGTSSSVASLQTPPSPDQE
NHIDNELENYDTSLSDVSTPNKKEGDEFEQSLRDTFASFRKTKPPPPLDFEQPRLPST
ASSSVDSTVSSPLTDEDIKELEFLPNESTHSYSYNPLSPNSLAVRLRILKRSLEIIIQ
NPSMLLEPTPDDLPPLKEFAGRRSSLPRTSASANHLMNRNKSQIWNTTSATLNAFVNN
TSSSSAASSALSNKKPGTPVFPNLDPTHSQTFHRANSLAYLPSILPEQDPLLKHNNSL
FRGDYGNNISPERPSFRQPFKDQTSNLRNSSLLNERAYQEDETFLPHHGPSMDLLNEQ
RANLKSLLNLLNETLEKNTSERASDLHMISLFNLNKLMLGDPKKNNSERDKRTEKLKK
ILLDSLAEPFFEHYNFIGDNPIADTDELKEEIDEFTGSGDTTAITDIRPQQDYGRILR
TFTSTKNSAPQAIFTCSQEDPWQFRAANDLACLVFGISQNAIRALTLMDLIHTDSRNF
VLHKLLSTEGQEMVFTGEIIGIVQPETLSSSKVVWASFWAKRKNGLLVCVFEKVPCDY
VDVLLNLDDFGAENIVDKCELLSDGPTLSSSSTLSLPKMASSPTGSKLEYSLERKILE
KSYTKPTSTENRNGDENQLDGDSHSEPSLSSSPVRSKKSVKFANDIKDVKSISQSLAK
LMDDVRNGVVFDPDDDLLPMPIKVCNHINETRYFTLNHLSYNIPCAVSSTVLEDELKL
KIHSLPYQAGLFIVDSHTLDIVSSNKSILKNMFGYHFAELVGKSITEIIPSFPKFLQF
INDKYPALDITLHKNKGLVLTEHFFRKIQAEIMGDRKSFYTSVGIDGLHRDGCEIKID
FQLRVMNSKVILLWVTHSRDVVFEEYNTNPSQLKMLKESELSLMSSASSSASSSKKSS
SRISTGTLKDMSNLSTYEDLAHRTNKLKYEIGDDSRAHSQSTLSEQEQVPLENDKDSG
EMMLADPEMKHKLELARIYSRDKSQFVKEGNFKVDENLIISKISLSPSTESLADSKSS
GKGLSPLEEEKLIDENATENGLAGSPKDEDGIIMTNKRGNQPVSTFLRTPEKNIGAQK
HVKKFSDFVSLQKMGEGAYGKVNLCIHKKNRYIVVIKMIFKERILVDTWVRDRKLGTI
PSEIQIMATLNKKPHENILRLLDFFEDDDYYYIETPVHGETGCIDLFDLIEFKTNMTE
FEAKLIFKQVVAGIKHLHDQGIVHRDIKDENVIVDSKGFVKIIDFGSAAYVKSGPFDV
FVGTIDYAAPEVLGGNPYEGQPQDIWAIGILLYTVVFKENPFYNIDEILEGDLKFNNA
EEVSEDCIELIKSILNRCVPKRPTIDDINNDKWLVI"
rep_origin 124349..124597
/note="ARS107; Autonomously Replicating Sequence"
/db_xref="SGD:S000118319"
gene <124879..>126786
/gene="TPD3"
/locus_tag="YAL016W"
/gene_synonym="FUN32"
/db_xref="GeneID:851217"
mRNA <124879..>126786
/gene="TPD3"
/locus_tag="YAL016W"
/gene_synonym="FUN32"
/product="protein phosphatase 2A structural subunit TPD3"
/transcript_id="NM_001178161.1"
/db_xref="GeneID:851217"
CDS 124879..126786
/gene="TPD3"
/locus_tag="YAL016W"
/gene_synonym="FUN32"
/experiment="EXISTENCE:direct assay:GO:0000159 protein
phosphatase type 2A complex [PMID:17550305|PMID:8600023]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12388751]"
/experiment="EXISTENCE:direct assay:GO:0043332 mating
projection tip [PMID:12388751]"
/experiment="EXISTENCE:mutant phenotype:GO:0004722 protein
serine/threonine phosphatase activity [PMID:17550305]"
/experiment="EXISTENCE:mutant phenotype:GO:0006417
regulation of translation [PMID:10329624]"
/experiment="EXISTENCE:mutant phenotype:GO:0007094 mitotic
spindle assembly checkpoint signaling [PMID:9001215]"
/note="Regulatory subunit A of the heterotrimeric PP2A
complex; the heterotrimeric protein phosphatase 2A (PP2A)
complex also contains regulatory subunit Cdc55p and either
catalytic subunit Pph21p or Pph22p; required for cell
morphogenesis and transcription by RNA polymerase III"
/codon_start=1
/product="protein phosphatase 2A structural subunit TPD3"
/protein_id="NP_009386.1"
/db_xref="GeneID:851217"
/db_xref="SGD:S000000014"
/translation="MSGARSTTAGAVPSAATTSTTSTTSNSKDSDSNESLYPLALLMD
ELKHDDIANRVEAMKKLDTIALALGPERTRNELIPFLTEVAQDDEDEVFAVLAEQLGK
FVPYIGGPQYATILLPVLEILASAEETLVREKAVDSLNNVAQELSQEQLFSDFVPLIE
HLATADWFSSKVSACGLFKSVIVRIKDDSLRKNILALYLQLAQDDTPMVKRAVGKNLP
ILIDLLTQNLGLSTDEDWDYISNIFQKIINDNQDSVKFLAVDCLISILKFFNAKGDES
HTQDLLNSAVKLIGDEAWRVRYMAADRFSDLASQFSSNQAYIDELVQPFLNLCEDNEG
DVREAVAKQVSGFAKFLNDPSIILNKILPAVQNLSMDESETVRSALASKITNIVLLLN
KDQVINNFLPILLNMLRDEFPDVRLNIIASLKVVNDVIGIELLSDSLLPAITELAKDV
NWRVRMAIIEYIPILAEQLGMQFFDQQLSDLCLSWLWDTVYSIREAAVNNLKRLTEIF
GSDWCRDEIISRLLKFDLQLLENFVSRFTILSALTTLVPVVSLDVVTEQLLPFISHLA
DDGVPNIRFNVAKSYAVIVKVLIKDEAKYDALIKNTILPSLQTLCQDEDVDVKYFAKK
SLAECQELLKN"
gene complement(<126903..>128102)
/gene="NTG1"
/locus_tag="YAL015C"
/gene_synonym="FUN33; ogg2; SCR1"
/db_xref="GeneID:851218"
mRNA complement(<126903..>128102)
/gene="NTG1"
/locus_tag="YAL015C"
/gene_synonym="FUN33; ogg2; SCR1"
/product="bifunctional N-glycosylase/AP lyase NTG1"
/transcript_id="NM_001178160.1"
/db_xref="GeneID:851218"
CDS complement(126903..128102)
/gene="NTG1"
/locus_tag="YAL015C"
/gene_synonym="FUN33; ogg2; SCR1"
/EC_number="4.2.99.18"
/experiment="EXISTENCE:direct assay:GO:0000703 oxidized
pyrimidine nucleobase lesion DNA N-glycosylase activity
[PMID:9020769]"
/experiment="EXISTENCE:direct assay:GO:0003906
DNA-(apurinic or apyrimidinic site) endonuclease activity
[PMID:14500818]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:24034606|PMID:19029246|PMID:10207101]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:24034606|PMID:19029246|PMID:16823961|P
MID:10207101]"
/experiment="EXISTENCE:direct assay:GO:0006281 DNA repair
[PMID:8855249]"
/experiment="EXISTENCE:direct assay:GO:0006284
base-excision repair [PMID:14500818]"
/experiment="EXISTENCE:direct assay:GO:0006285
base-excision repair, AP site formation [PMID:9826748]"
/experiment="EXISTENCE:direct assay:GO:0008534 oxidized
purine nucleobase lesion DNA N-glycosylase activity
[PMID:8855249]"
/experiment="EXISTENCE:mutant phenotype:GO:0034599
cellular response to oxidative stress [PMID:19074198]"
/experiment="EXISTENCE:mutant phenotype:GO:0090297
positive regulation of mitochondrial DNA replication
[PMID:17116696|PMID:19074198]"
/note="DNA N-glycosylase and apurinic/apyrimidinic (AP)
lyase; involved in base excision repair; acts in both
nucleus and mitochondrion; creates a double-strand break
at mtDNA origins that stimulates replication in response
to oxidative stress; required for maintaining
mitochondrial genome integrity; NTG1 has a paralog, NTG2,
that arose from the whole genome duplication"
/codon_start=1
/product="bifunctional N-glycosylase/AP lyase NTG1"
/protein_id="NP_009387.1"
/db_xref="GeneID:851218"
/db_xref="SGD:S000000013"
/translation="MQKISKYSSMAILRKRPLVKTETGPESELLPEKRTKIKQEEVVP
QPVDIDWVKSLPNKQYFEWIVVRNGNVPNRWATPLDPSILVTPASTKVPYKFQETYAR
MRVLRSKILAPVDIIGGSSIPVTVASKCGISKEQISPRDYRLQVLLGVMLSSQTKDEV
TAMAMLNIMRYCIDELHSEEGMTLEAVLQINETKLDELIHSVGFHTRKAKYILSTCKI
LQDQFSSDVPATINELLGLPGVGPKMAYLTLQKAWGKIEGICVDVHVDRLTKLWKWVD
AQKCKTPDQTRTQLQNWLPKGLWTEINGLLVGFGQIITKSRNLGDMLQFLPPDDPRSS
LDWDLQSQLYKEIQQNIMSYPKWVKYLEGKRELNVEAEINVKHEEKTVEETMVKLEND
ISVKVED"
gene complement(<128252..>129019)
/gene="SYN8"
/locus_tag="YAL014C"
/gene_synonym="SLT2; UIP2"
/db_xref="GeneID:851219"
mRNA complement(<128252..>129019)
/gene="SYN8"
/locus_tag="YAL014C"
/gene_synonym="SLT2; UIP2"
/product="syntaxin"
/transcript_id="NM_001178159.1"
/db_xref="GeneID:851219"
CDS complement(128252..129019)
/gene="SYN8"
/locus_tag="YAL014C"
/gene_synonym="SLT2; UIP2"
/experiment="EXISTENCE:direct assay:GO:0005484 SNAP
receptor activity [PMID:12453154]"
/experiment="EXISTENCE:direct assay:GO:0005768 endosome
[PMID:12453154]"
/experiment="EXISTENCE:mutant phenotype:GO:0006896 Golgi
to vacuole transport [PMID:12453154]"
/experiment="EXISTENCE:physical interaction:GO:0006896
Golgi to vacuole transport [PMID:12453154]"
/note="Endosomal SNARE related to mammalian syntaxin 8"
/codon_start=1
/product="syntaxin"
/protein_id="NP_009388.2"
/db_xref="GeneID:851219"
/db_xref="SGD:S000000012"
/translation="MDVLKLGYELDQLSDLVEERTRLVSVLKLAPTSNDNVTLKRQLG
SILELLQKCAPNDELISRYNTILDKIPDTAVDKELYRFQQQVARNTDEVSKESLKKVR
FKNDDELTVMYKDDDEQDEESPLPSTHTPYKDEPLQSQLQSQSQPQPPQPMVSNQELF
INQQQQLLEQDSHLGALSQSIGRTHDISLDLNNEIVSQNDSLLVDLENLIDNNGRNLN
RASRSMHGFNNSRFKDNGNCVIILVLIVVLLLLLLVL"
gene <129270..>130487
/gene="DEP1"
/locus_tag="YAL013W"
/gene_synonym="FUN54"
/db_xref="GeneID:851220"
mRNA <129270..>130487
/gene="DEP1"
/locus_tag="YAL013W"
/gene_synonym="FUN54"
/product="Rpd3L histone deacetylase complex subunit DEP1"
/transcript_id="NM_001178158.2"
/db_xref="GeneID:851220"
CDS 129270..130487
/gene="DEP1"
/locus_tag="YAL013W"
/gene_synonym="FUN54"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:36283334]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:36283334]"
/experiment="EXISTENCE:direct assay:GO:0033698 Rpd3L
complex
[PMID:16286007|PMID:16314178|PMID:16286008|PMID:19040720]"
/experiment="EXISTENCE:direct assay:GO:0070210
Rpd3L-Expanded complex [PMID:19040720]"
/experiment="EXISTENCE:mutant phenotype:GO:0000122
negative regulation of transcription by RNA polymerase II
[PMID:8056324|PMID:24358376|PMID:16314178]"
/experiment="EXISTENCE:mutant phenotype:GO:0006357
regulation of transcription by RNA polymerase II
[PMID:21646424|PMID:8056324]"
/experiment="EXISTENCE:mutant phenotype:GO:0030174
regulation of DNA-templated DNA replication initiation
[PMID:19417103]"
/experiment="EXISTENCE:mutant phenotype:GO:0034605
cellular response to heat [PMID:20398213]"
/experiment="EXISTENCE:mutant phenotype:GO:0045944
positive regulation of transcription by RNA polymerase II
[PMID:20398213|PMID:8056324]"
/experiment="EXISTENCE:mutant phenotype:GO:0061186
negative regulation of silent mating-type cassette
heterochromatin formation [PMID:16286008]"
/experiment="EXISTENCE:mutant phenotype:GO:0061188
negative regulation of rDNA heterochromatin formation
[PMID:16286008]"
/experiment="EXISTENCE:mutant phenotype:GO:1900089
negative regulation of inositol biosynthetic process
[PMID:8056324]"
/experiment="EXISTENCE:mutant phenotype:GO:1900090
positive regulation of inositol biosynthetic process
[PMID:8056324]"
/experiment="EXISTENCE:mutant phenotype:GO:1900469
negative regulation of phosphatidylserine biosynthetic
process [PMID:8056324]"
/experiment="EXISTENCE:mutant phenotype:GO:1900470
positive regulation of phosphatidylserine biosynthetic
process [PMID:8056324]"
/experiment="EXISTENCE:mutant phenotype:GO:2001246
negative regulation of phosphatidylcholine biosynthetic
process [PMID:8056324]"
/experiment="EXISTENCE:mutant phenotype:GO:2001247
positive regulation of phosphatidylcholine biosynthetic
process [PMID:8056324]"
/note="Component of the Rpd3L histone deacetylase complex;
required for diauxic shift-induced histone H2B deposition
onto rDNA genes; transcriptional modulator involved in
regulation of structural phospholipid biosynthesis genes
and metabolically unrelated genes, as well as maintenance
of telomeres, mating efficiency, and sporulation;
localizes to both the nucleus and mitochondria where it
may play a role in mitophagy"
/codon_start=1
/product="Rpd3L histone deacetylase complex subunit DEP1"
/protein_id="NP_009389.3"
/db_xref="GeneID:851220"
/db_xref="SGD:S000000011"
/translation="MSQQTPQESEQTTAKEQDLDQESVLSNIDFNTDLNHNLNLSEYC
ISSDAGTEKMDSDEEKSLANLPELKYAPKLSSLVKQETLTESLKRPHEDEKEAIDEAK
KMKVPGENEDESKEEEKSQELEEAIDSKEKSTDARDEQGDEGDNEEENNEEDNENENE
HTAPPALVMPSPIEMEEQRMTALKEITDIEYKFAQLRQKLYDNQLVRLQTELQMCLEG
SHPELQVYYSKIAAIRDYKLHRAYQRQKYELSCINTETIATRTFIHQDFHKKVTDLRA
RLLNRTTQTWYDINKERRDMDIVIPDVNYHVPIKLDNKTLSCITGYASAAQLCYPGEP
VAEDLACESIEYRYRANPVDKLEVIVDRMRLNNEISDLEGLRKYFHSFPGAPELNPLR
DSEINDDFHQWAQ"
gene <130799..>131983
/gene="CYS3"
/locus_tag="YAL012W"
/gene_synonym="CYI1; FUN35; STR1"
/db_xref="GeneID:851221"
mRNA <130799..>131983
/gene="CYS3"
/locus_tag="YAL012W"
/gene_synonym="CYI1; FUN35; STR1"
/product="cystathionine gamma-lyase CYS3"
/transcript_id="NM_001178157.1"
/db_xref="GeneID:851221"
CDS 130799..131983
/gene="CYS3"
/locus_tag="YAL012W"
/gene_synonym="CYI1; FUN35; STR1"
/EC_number="4.4.1.1"
/experiment="EXISTENCE:direct assay:GO:0004123
cystathionine gamma-lyase activity [PMID:1577698]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095|PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0047804
cysteine-S-conjugate beta-lyase activity [PMID:29436228]"
/experiment="EXISTENCE:direct assay:GO:1904828 positive
regulation of hydrogen sulfide biosynthetic process
[PMID:31582588]"
/experiment="EXISTENCE:mutant phenotype:GO:0004123
cystathionine gamma-lyase activity
[PMID:8366024|PMID:10509018]"
/experiment="EXISTENCE:mutant phenotype:GO:0019343
L-cysteine biosynthetic process via L-cystathionine
[PMID:10509018]"
/experiment="EXISTENCE:mutant phenotype:GO:0019344
L-cysteine biosynthetic process [PMID:8366024]"
/experiment="EXISTENCE:mutant phenotype:GO:0019346
transsulfuration [PMID:8366024]"
/note="Cystathionine gamma-lyase; catalyzes one of the two
reactions involved in the transsulfuration pathway that
yields cysteine from homocysteine with the intermediary
formation of cystathionine; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="cystathionine gamma-lyase CYS3"
/protein_id="NP_009390.1"
/db_xref="GeneID:851221"
/db_xref="SGD:S000000010"
/translation="MTLQESDKFATKAIHAGEHVDVHGSVIEPISLSTTFKQSSPANP
IGTYEYSRSQNPNRENLERAVAALENAQYGLAFSSGSATTATILQSLPQGSHAVSIGD
VYGGTHRYFTKVANAHGVETSFTNDLLNDLPQLIKENTKLVWIETPTNPTLKVTDIQK
VADLIKKHAAGQDVILVVDNTFLSPYISNPLNFGADIVVHSATKYINGHSDVVLGVLA
TNNKPLYERLQFLQNAIGAIPSPFDAWLTHRGLKTLHLRVRQAALSANKIAEFLAADK
ENVVAVNYPGLKTHPNYDVVLKQHRDALGGGMISFRIKGGAEAASKFASSTRLFTLAE
SLGGIESLLEVPAVMTHGGIPKEAREASGVFDDLVRISVGIEDTDDLLEDIKQALKQA
TN"
gene <132199..>134076
/gene="SWC3"
/locus_tag="YAL011W"
/gene_synonym="SWC1"
/db_xref="GeneID:851222"
mRNA <132199..>134076
/gene="SWC3"
/locus_tag="YAL011W"
/gene_synonym="SWC1"
/product="Swc3p"
/transcript_id="NM_001178156.1"
/db_xref="GeneID:851222"
CDS 132199..134076
/gene="SWC3"
/locus_tag="YAL011W"
/gene_synonym="SWC1"
/experiment="EXISTENCE:direct assay:GO:0000812 Swr1
complex [PMID:14690608|PMID:14645854]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961|PMID:14576278]"
/experiment="EXISTENCE:direct assay:GO:0006338 chromatin
remodeling [PMID:14645854]"
/experiment="EXISTENCE:direct assay:GO:0031492 nucleosomal
DNA binding [PMID:39357520]"
/experiment="EXISTENCE:mutant phenotype:GO:0006338
chromatin remodeling [PMID:14690608]"
/experiment="EXISTENCE:mutant phenotype:GO:0007029
endoplasmic reticulum organization [PMID:12868057]"
/note="hypothetical protein; component of the SWR1
complex, which exchanges histone variant H2AZ (Htz1p) for
chromatin-bound histone H2A; required for formation of
nuclear-associated array of smooth endoplasmic reticulum
known as karmellae"
/codon_start=1
/product="Swc3p"
/protein_id="NP_009391.2"
/db_xref="GeneID:851222"
/db_xref="SGD:S000000009"
/translation="MPAVLRTRSKESSIEQKPASRTRTRSRRGKRGRDDDDDDDDEES
DDAYDEVGNDYDEYASRAKLATNRPFEIVAGLPASVELPNYNSSLTHPQSIKNSGVLY
DSLVSSRRTWVQGEMFELYWRRPKKIVSESTPAATESPTSGTIPLIRDKMQKMCDCVM
SGGPHTFKVRLFILKNDKIEQKWQDEQELKKKEKELKRKNDAEAKRLRMEERKRQQMQ
KKIAKEQKLQLQKENKAKQKLEQEALKLKRKEEMKKLKEQNKNKQGSPSSSMHDPRMI
MNLNLMAQEDPKLNTLMETVAKGLANNSQLEEFKKFIEIAKKRSLEENPVNKRPSVTT
TRPAPPSKAKDVAEDHRLNSITLVKSSKTAATEPEPKKADDENAEKQQSKEAKTTAES
TQVDVKKEEEDVKEKGVKSEDTQKKEDNQVVPKRKRRKNAIKEDKDMQLTAFQQKYVQ
GAEIILEYLEFTHSRYYLPKKSVVEFLEDTDEIIISWIVIHNSKEIEKFKTKKIKAKL
KADQKLNKEDAKPGSDVEKEVSFNPLFEADCPTPLYTPMTMKLSGIHKRFNQIIRNSV
SPMEEVVKEMEKILQIGTRLSGYNLWYQLDGYDDEALSESLRFELNEWEHAMRSRRHK
R"
gene complement(<134184..>135665)
/gene="MDM10"
/locus_tag="YAL010C"
/gene_synonym="FUN37"
/db_xref="GeneID:851223"
mRNA complement(<134184..>135665)
/gene="MDM10"
/locus_tag="YAL010C"
/gene_synonym="FUN37"
/product="Mdm10p"
/transcript_id="NM_001178155.2"
/db_xref="GeneID:851223"
CDS complement(134184..135665)
/gene="MDM10"
/locus_tag="YAL010C"
/gene_synonym="FUN37"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407|PMID:8089171]"
/experiment="EXISTENCE:genetic interaction:GO:0015914
phospholipid transport [PMID:19556461]"
/experiment="EXISTENCE:mutant phenotype:GO:0007005
mitochondrion organization [PMID:19556461]"
/experiment="EXISTENCE:mutant phenotype:GO:0007031
peroxisome organization [PMID:30908556]"
/experiment="EXISTENCE:mutant phenotype:GO:0045040 protein
insertion into mitochondrial outer membrane
[PMID:17410204]"
/experiment="EXISTENCE:mutant phenotype:GO:0051654
establishment of mitochondrion localization
[PMID:13679517]"
/experiment="EXISTENCE:mutant phenotype:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:20026336|PMID:15239954]"
/experiment="EXISTENCE:mutant phenotype:GO:1990456
mitochondrion-endoplasmic reticulum membrane tethering
[PMID:19556461]"
/experiment="EXISTENCE:physical interaction:GO:0001401 SAM
complex [PMID:17410204]"
/experiment="EXISTENCE:physical interaction:GO:0032865
ERMES complex [PMID:17410204|PMID:13679517]"
/experiment="EXISTENCE:physical interaction:GO:0070096
mitochondrial outer membrane translocase complex assembly
[PMID:20026336]"
/note="Moonlighting protein component of both the ERMES
and SAM complex; subunit of the ERMES complex that acts as
a molecular tether between the mitochondria and the ER,
necessary for both efficient phospholipid exchange between
organelles and for mitophagy; SAM/TOB complex subunit that
functions in the assembly of outer membrane beta-barrel
proteins; involved in mitochondrial inheritance and
morphology; ERMES complex often co-localizes with
peroxisomes adjacent to mitochondria-ER junctions"
/codon_start=1
/product="Mdm10p"
/protein_id="NP_009392.2"
/db_xref="GeneID:851223"
/db_xref="SGD:S000000008"
/translation="MLPYMDQVLRAFYQSTHWSTQNSYEDITATSRTLLDFRIPSAIH
LQISNKSTPNTFNSLDFSTRSRINGSLSYLYSDAQQLEKFMRNSTDIPLQDATETYRQ
LQPNLNFSVSSANTLSSDNTTVDNDKKLLHDSKFVKKSLYYGRMYYPSSDLEAMIIKR
LSPQTQFMLKGVSSFKESLNVLTCYFQRDSHRNLQEWIFSTSDLLCGYRVLHNFLTTP
SKFNTSLYNNSSLSLGAEFWLGLVSLSPGCSTTLRYYTHSTNTGRPLTLTLSWNPLFG
HISSTYSAKTGTNSTFCAKYDFNLYSIESNLSFGCEFWQKKHHLLETNKNNNDKLEPI
SDELVDINPNSRATKLLHENVPDLNSAVNDIPSTLDIPVHKQKLLNDLTYAFSSSLRK
IDEERSTIEKFDNKINSSIFTSVWKLSTSLRDKTLKLLWEGKWRGFLISAGTELVFTR
GFQESLSDDEKNDNAISISATDTENGNIPVFPAKFGIQFQYST"
gene <135854..>136633
/gene="SPO7"
/locus_tag="YAL009W"
/db_xref="GeneID:851224"
mRNA <135854..>136633
/gene="SPO7"
/locus_tag="YAL009W"
/product="Nem1-Spo7 phosphatase regulatory subunit SPO7"
/transcript_id="NM_001178154.1"
/db_xref="GeneID:851224"
CDS 135854..136633
/gene="SPO7"
/locus_tag="YAL009W"
/experiment="EXISTENCE:direct assay:GO:0004721
phosphoprotein phosphatase activity
[PMID:15889145|PMID:25359770]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:9822591]"
/experiment="EXISTENCE:direct assay:GO:0061709
reticulophagy [PMID:31802527]"
/experiment="EXISTENCE:genetic interaction:GO:0071072
negative regulation of phospholipid biosynthetic process
[PMID:15889145]"
/experiment="EXISTENCE:mutant phenotype:GO:0006998 nuclear
envelope organization [PMID:9822591]"
/experiment="EXISTENCE:mutant phenotype:GO:0071071
regulation of phospholipid biosynthetic process
[PMID:30201607]"
/experiment="EXISTENCE:physical interaction:GO:0071595
Nem1-Spo7 phosphatase complex [PMID:9822591]"
/note="Putative regulatory subunit of Nem1p-Spo7p
phosphatase holoenzyme; regulates nuclear/ER membrane
association and activation of Pah1p, a phosphatidate
phosphatase involved in the production of diacylglycerol
DAG for lipid droplet biogenesis; phosphatase activity of
the Nem1p-Spo7p complex is inhibited by Ice2p; regulates
nuclear growth by controlling phospholipid biosynthesis;
required for normal nuclear envelope morphology,
premeiotic replication, and sporulation"
/codon_start=1
/product="Nem1-Spo7 phosphatase regulatory subunit SPO7"
/protein_id="NP_009393.1"
/db_xref="GeneID:851224"
/db_xref="SGD:S000000007"
/translation="MEPESIGDVGNHAQDDSASIVSGPRRRSTSKTSSAKNIRNSSNI
SPASMIFRNLLILEDDLRRQAHEQKILKWQFTLFLASMAGVGAFTFYELYFTSDYVKG
LHRVILQFTLSFISITVVLFHISGQYRRTIVIPRRFFTSTNKGIRQFNVKLVKVQSTW
DEKYTDSVRFVSRTIAYCNIYCLKKFLWLKDDNAIVKFWKSVTIQSQPRIGAVDVKLV
LNPRAFSAEIREGWEIYRDEFWAREGARRRKQAHELRPKSE"
gene <136914..>137510
/gene="FUN14"
/locus_tag="YAL008W"
/gene_synonym="MCP3"
/db_xref="GeneID:851225"
mRNA <136914..>137510
/gene="FUN14"
/locus_tag="YAL008W"
/gene_synonym="MCP3"
/product="Fun14p"
/transcript_id="NM_001178153.1"
/db_xref="GeneID:851225"
CDS 136914..137510
/gene="FUN14"
/locus_tag="YAL008W"
/gene_synonym="MCP3"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:16823961|PMID:14562095|PMID:14576278|PMID:24769239]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane
[PMID:27226123|PMID:16407407]"
/experiment="EXISTENCE:genetic interaction:GO:0007005
mitochondrion organization [PMID:27226123]"
/experiment="EXISTENCE:genetic interaction:GO:0055091
phospholipid homeostasis [PMID:27226123]"
/note="Integral mitochondrial outer membrane (MOM)
protein; dosage suppressor of an MDM10 null that reduces
ERMES-related phenotypes, such as alterations in
mitochondrial morphology, protein complex assembly, and
lipid profile; dosage suppressor of MDM12, MDM34, and MMM1
null mutant growth defects; novel mechanism of MOM import
involving Tom70p, the TOM complex, and the TIM23 complex,
requiring mitochondrial membrane potential and processing
by the IMP complex for correct biogenesis"
/codon_start=1
/product="Fun14p"
/protein_id="NP_009394.1"
/db_xref="GeneID:851225"
/db_xref="SGD:S000000006"
/translation="MTLAFNMQRLVFRNLNVGKRMFKNVPLWRFNVANKLGKPLTRSV
GLGGAGIVAGGFYLMNRQPSKLIFNDSLGAAVKQQGPLEPTVGNSTAITEERRNKISS
HKQMFLGSLFGVVLGVTVAKISILFMYVGITSMLLCEWLRYKGWIRINLKNIKSVIVL
KDVDLKKLLIDGLLGTEYMGFKVFFTLSFVLASLNANK"
gene complement(<137698..>138345)
/gene="ERP2"
/locus_tag="YAL007C"
/db_xref="GeneID:851226"
mRNA complement(<137698..>138345)
/gene="ERP2"
/locus_tag="YAL007C"
/product="Erp2p"
/transcript_id="NM_001178152.1"
/db_xref="GeneID:851226"
CDS complement(137698..138345)
/gene="ERP2"
/locus_tag="YAL007C"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:11157978]"
/experiment="EXISTENCE:mutant phenotype:GO:0006621 protein
retention in ER lumen [PMID:10359606]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10359606]"
/note="Member of the p24 family involved in ER to Golgi
transport; similar to Emp24p and Erv25p; role in misfolded
protein quality control; forms a heterotrimeric complex
with Erp1p, Emp24p, and Erv25p; localized to COPII-coated
vesicles; ERP2 has a paralog, ERP4, that arose from the
whole genome duplication"
/codon_start=1
/product="Erp2p"
/protein_id="NP_009395.1"
/db_xref="GeneID:851226"
/db_xref="SGD:S000000005"
/translation="MIKSTIALPSFFIVLILALVNSVAASSSYAPVAISLPAFSKECL
YYDMVTEDDSLAVGYQVLTGGNFEIDFDITAPDGSVITSEKQKKYSDFLLKSFGVGKY
TFCFSNNYGTALKKVEITLEKEKTLTDEHEADVNNDDIIANNAVEEIDRNLNKITKTL
NYLRAREWRNMSTVNSTESRLTWLSILIIIIIAVISIAQVLLIQFLFTGRQKNYV"
repeat_region complement(138831..138992)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006786"
gene 139152..139254
/gene="TRN1"
/locus_tag="YNCA0002W"
/db_xref="GeneID:851227"
tRNA join(139152..139187,139219..139254)
/gene="TRN1"
/locus_tag="YNCA0002W"
/product="tRNA-Pro"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Proline tRNA (tRNA-Pro), predicted by tRNAscan-SE
analysis; target of K. lactis zymocin; can mutate to
suppress +1 frameshift mutations in proline codons"
/db_xref="GeneID:851227"
/db_xref="SGD:S000006680"
gene complement(<139503..>141431)
/gene="SSA1"
/locus_tag="YAL005C"
/gene_synonym="YG100"
/db_xref="GeneID:851259"
mRNA complement(<139503..>141431)
/gene="SSA1"
/locus_tag="YAL005C"
/gene_synonym="YG100"
/product="Hsp70 family ATPase SSA1"
/transcript_id="NM_001178151.1"
/db_xref="GeneID:851259"
CDS complement(139503..141431)
/gene="SSA1"
/locus_tag="YAL005C"
/gene_synonym="YG100"
/experiment="EXISTENCE:direct assay:GO:0000049 tRNA
binding [PMID:25853343]"
/experiment="EXISTENCE:direct assay:GO:0000209 protein
polyubiquitination [PMID:20462952]"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:10745074]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:11914276|PMID:10347213]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:11914276|PMID:8755907]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:16622836]"
/experiment="EXISTENCE:direct assay:GO:0006457 protein
folding [PMID:8947547]"
/experiment="EXISTENCE:direct assay:GO:0006606 protein
import into nucleus [PMID:10347213]"
/experiment="EXISTENCE:direct assay:GO:0006616
SRP-dependent cotranslational protein targeting to
membrane, translocation [PMID:8754838]"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:8755907]"
/experiment="EXISTENCE:direct assay:GO:0016887 ATP
hydrolysis activity [PMID:7737974|PMID:18706386]"
/experiment="EXISTENCE:direct assay:GO:0034605 cellular
response to heat [PMID:24291094]"
/experiment="EXISTENCE:direct assay:GO:0035617 stress
granule disassembly [PMID:24291094]"
/experiment="EXISTENCE:direct assay:GO:0042026 protein
refolding [PMID:18706386|PMID:9674429]"
/experiment="EXISTENCE:direct assay:GO:0051082 unfolded
protein binding [PMID:9789005]"
/experiment="EXISTENCE:direct assay:GO:0072318 clathrin
coat disassembly [PMID:23913685]"
/experiment="EXISTENCE:genetic interaction:GO:0006606
protein import into nucleus [PMID:10347213]"
/experiment="EXISTENCE:genetic interaction:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:27178214]"
/experiment="EXISTENCE:mutant phenotype:GO:0002181
cytoplasmic translation [PMID:11279042]"
/experiment="EXISTENCE:mutant phenotype:GO:0006626 protein
targeting to mitochondrion [PMID:8754838]"
/experiment="EXISTENCE:mutant phenotype:GO:0043161
proteasome-mediated ubiquitin-dependent protein catabolic
process [PMID:27178214]"
/experiment="EXISTENCE:mutant phenotype:GO:0072671
mitochondria-associated ubiquitin-dependent protein
catabolic process [PMID:32118579]"
/note="ATPase involved in protein folding and NLS-directed
nuclear transport; HSP70 family member; required for
Ub-dependent degradation of short-lived proteins; Ydj1p
accelerates ATP hydrolysis and release;
mechanosensor-mediated phosphorylation by Pkc1p promotes
the global heat shock response; localizes to the nucleus,
cytoplasm and cell wall; role in [URE3] prion propagation
and vacuolar-mediated degradation of gluconeogenic
enzymes; targets Hsp104p to prion fibrils; 98% identity
with paralog Ssa2p"
/codon_start=1
/product="Hsp70 family ATPase SSA1"
/protein_id="NP_009396.2"
/db_xref="GeneID:851259"
/db_xref="SGD:S000000004"
/translation="MSKAVGIDLGTTYSCVAHFANDRVDIIANDQGNRTTPSFVAFTD
TERLIGDAAKNQAAMNPSNTVFDAKRLIGRNFNDPEVQADMKHFPFKLIDVDGKPQIQ
VEFKGETKNFTPEQISSMVLGKMKETAESYLGAKVNDAVVTVPAYFNDSQRQATKDAG
TIAGLNVLRIINEPTAAAIAYGLDKKGKEEHVLIFDLGGGTFDVSLLSIEDGIFEVKA
TAGDTHLGGEDFDNRLVNHFIQEFKRKNKKDLSTNQRALRRLRTACERAKRTLSSSAQ
TSVEIDSLFEGIDFYTSITRARFEELCADLFRSTLDPVEKVLRDAKLDKSQVDEIVLV
GGSTRIPKVQKLVTDYFNGKEPNRSINPDEAVAYGAAVQAAILTGDESSKTQDLLLLD
VAPLSLGIETAGGVMTKLIPRNSTIPTKKSEIFSTYADNQPGVLIQVFEGERAKTKDN
NLLGKFELSGIPPAPRGVPQIEVTFDVDSNGILNVSAVEKGTGKSNKITITNDKGRLS
KEDIEKMVAEAEKFKEEDEKESQRIASKNQLESIAYSLKNTISEAGDKLEQADKDTVT
KKAEETISWLDSNTTASKEEFDDKLKELQDIANPIMSKLYQAGGAPGGAAGGAPGGFP
GGAPPAPEAEGPTVEEVD"
gene <142174..>143160
/gene="EFB1"
/locus_tag="YAL003W"
/gene_synonym="TEF5"
/db_xref="GeneID:851260"
mRNA join(<142174..142253,142620..>143160)
/gene="EFB1"
/locus_tag="YAL003W"
/gene_synonym="TEF5"
/product="translation elongation factor 1 subunit beta"
/transcript_id="NM_001178150.1"
/db_xref="GeneID:851260"
CDS join(142174..142253,142620..143160)
/gene="EFB1"
/locus_tag="YAL003W"
/gene_synonym="TEF5"
/experiment="EXISTENCE:direct assay:GO:0005085
guanyl-nucleotide exchange factor activity
[PMID:17925388]"
/experiment="EXISTENCE:direct assay:GO:0032232 negative
regulation of actin filament bundle assembly
[PMID:19095653]"
/experiment="EXISTENCE:genetic interaction:GO:0006449
regulation of translational termination [PMID:19545407]"
/experiment="EXISTENCE:mutant phenotype:GO:0005853
eukaryotic translation elongation factor 1 complex
[PMID:8420802]"
/experiment="EXISTENCE:mutant phenotype:GO:0006414
translational elongation [PMID:10409717]"
/experiment="EXISTENCE:mutant phenotype:GO:1990145
maintenance of translational fidelity [PMID:10409717]"
/note="Translation elongation factor 1 beta; stimulates
nucleotide exchange to regenerate EF-1 alpha-GTP for the
next elongation cycle; part of the EF-1 complex, which
facilitates binding of aminoacyl-tRNA to the ribosomal A
site; human homolog EEF1B2 can complement yeast efb1
mutants"
/codon_start=1
/product="translation elongation factor 1 subunit beta"
/protein_id="NP_009398.1"
/db_xref="GeneID:851260"
/db_xref="SGD:S000000003"
/translation="MASTDFSKIETLKQLNASLADKSYIEGTAVSQADVTVFKAFQSA
YPEFSRWFNHIASKADEFDSFPAASAAAAEEEEDDDVDLFGSDDEEADAEAEKLKAER
IAAYNAKKAAKPAKPAAKSIVTLDVKPWDDETNLEEMVANVKAIEMEGLTWGAHQFIP
IGFGIKKLQINCVVEDDKVSLDDLQQSIEEDEDHVQSTDIAAMQKL"
gene 142367..142468
/gene="SNR18"
/locus_tag="YNCA0003W"
/db_xref="GeneID:9164867"
ncRNA 142367..142468
/ncRNA_class="snoRNA"
/gene="SNR18"
/locus_tag="YNCA0003W"
/product="SNR18"
/experiment="EXISTENCE:curator inference:GO:0005730
nucleolus [PMID:8674114]"
/experiment="EXISTENCE:direct assay:GO:0031428 box C/D
methylation guide snoRNP complex [PMID:10094313]"
/experiment="EXISTENCE:physical interaction:GO:0031428 box
C/D methylation guide snoRNP complex [PMID:10733567]"
/note="C/D box small nucleolar RNA (snoRNA); commonly
referred to as U18; guides 2'-O-methylation of large
subunit (LSU) rRNA at positions A649 and C650"
/transcript_id="NR_132150.1"
/db_xref="GeneID:9164867"
/db_xref="SGD:S000007500"
gene <143707..>147531
/gene="VPS8"
/locus_tag="YAL002W"
/gene_synonym="FUN15; VPL8; VPT8"
/db_xref="GeneID:851261"
mRNA <143707..>147531
/gene="VPS8"
/locus_tag="YAL002W"
/gene_synonym="FUN15; VPL8; VPT8"
/product="CORVET complex membrane-binding subunit VPS8"
/transcript_id="NM_001178149.1"
/db_xref="GeneID:851261"
CDS 143707..147531
/gene="VPS8"
/locus_tag="YAL002W"
/gene_synonym="FUN15; VPL8; VPT8"
/experiment="EXISTENCE:direct assay:GO:0005770 late
endosome [PMID:19828734]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:8969229]"
/experiment="EXISTENCE:direct assay:GO:0033263 CORVET
complex [PMID:17488625]"
/experiment="EXISTENCE:direct assay:GO:0051020 GTPase
binding [PMID:19828734]"
/experiment="EXISTENCE:mutant phenotype:GO:0006623 protein
targeting to vacuole [PMID:20173035]"
/experiment="EXISTENCE:mutant phenotype:GO:0007009 plasma
membrane organization [PMID:39002672]"
/experiment="EXISTENCE:mutant phenotype:GO:0032511 late
endosome to vacuole transport via multivesicular body
sorting pathway [PMID:19828734]"
/experiment="EXISTENCE:mutant phenotype:GO:0043495
protein-membrane adaptor activity [PMID:19828734]"
/experiment="EXISTENCE:physical interaction:GO:0051020
GTPase binding [PMID:19828734]"
/note="Membrane-binding component of the CORVET complex;
involved in endosomal vesicle tethering and fusion in the
endosome to vacuole protein targeting pathway; interacts
with Vps21p; contains RING finger motif"
/codon_start=1
/product="CORVET complex membrane-binding subunit VPS8"
/protein_id="NP_009399.2"
/db_xref="GeneID:851261"
/db_xref="SGD:S000000002"
/translation="MEQNGLDHDSRSSIDTTINDTQKTFLEFRSYTQLSEKLASSSSY
TAPPLNEDGPKGVASAVSQGSESVVSWTTLTHVYSILGAYGGPTCLYPTATYFLMGTS
KGCVLIFNYNEHLQTILVPTLSEDPSIHSIRSPVKSIVICSDGTHVAASYETGNICIW
NLNVGYRVKPTSEPTNGMTPTPALPAVLHIDDHVNKEITGLDFFGARHTALIVSDRTG
KVSLYNGYRRGFWQLVYNSKKILDVNSSKEKLIRSKLSPLISREKISTNLLSVLTTTH
FALILLSPHVSLMFQETVEPSVQNSLVVNSSISWTQNCSRVAYSVNNKISVISISSSD
FNVQSASHSPEFAESILSIQWIDQLLLGVLTISHQFLVLHPQHDFKILLRLDFLIHDL
MIPPNKYFVISRRSFYLLTNYSFKIGKFVSWSDITLRHILKGDYLGALEFIESLLQPY
CPLANLLKLDNNTEERTKQLMEPFYNLSLAALRFLIKKDNADYNRVYQLLMVVVRVLQ
QSSKKLDSIPSLDVFLEQGLEFFELKDNAVYFEVVANIVAQGSVTSISPVLFRSIIDY
YAKEENLKVIEDLIIMLNPTTLDVDLAVKLCQKYNLFDLLIYIWNKIFDDYQTPVVDL
IYRISNQSEKCVIFNGPQVPPETTIFDYVTYILTGRQYPQNLSISPSDKCSKIQRELS
AFIFSGFSIKWPSNSNHKLYICENPEEEPAFPYFHLLLKSNPSRFLAMLNEVFEASLF
NDDNDMVASVGEAELVSRQYVIDLLLDAMKDTGNSDNIRVLVAIFIATSISKYPQFIK
VSNQALDCVVNTICSSRVQGIYEISQIALESLLPYYHSRTTENFILELKEKNFNKVLF
HIYKSENKYASALSLILETKDIEKEYNTDIVSITDYILKKCPPGSLECGKVTEVIETN
FDLLLSRIGIEKCVTIFSDFDYNLHQEILEVKNEETQQKYLDKLFSTPNINNKVDKRL
RNLHIELNCKYKSKREMILWLNGTVLSNAESLQILDLLNQDSNFEAAAIIHERLESFN
LAVRDLLSFIEQCLNEGKTNISTLLESLRRAFDDCNSAGTEKKSCWILLITFLITLYG
KYPSHDERKDLCNKLLQEAFLGLVRSKSSSQKDSGGEFWEIMSSVLEHQDVILMKVQD
LKQLLLNVFNTYKLERSLSELIQKIIEDSSQDLVQQYRKFLSEGWSIHTDDCEICGKK
IWGAGLDPLLFLAWENVQRHQDMISVDLKTPLVIFKCHHGFHQTCLENLAQKPDEYSC
LICQTESNPKIV"
rep_origin 147398..147717
/note="ARS108; Autonomously Replicating Sequence"
/db_xref="SGD:S000121255"
gene complement(<147594..>151166)
/gene="TFC3"
/locus_tag="YAL001C"
/gene_synonym="FUN24; TSV115"
/db_xref="GeneID:851262"
mRNA complement(join(<147594..151006,151097..>151166))
/gene="TFC3"
/locus_tag="YAL001C"
/gene_synonym="FUN24; TSV115"
/product="transcription factor TFIIIC subunit TFC3"
/transcript_id="NM_001178148.1"
/db_xref="GeneID:851262"
CDS complement(join(147594..151006,151097..151166))
/gene="TFC3"
/locus_tag="YAL001C"
/gene_synonym="FUN24; TSV115"
/experiment="EXISTENCE:direct assay:GO:0000127
transcription factor TFIIIC complex
[PMID:2002052|PMID:1279682|PMID:2180956|PMID:1474578]"
/experiment="EXISTENCE:direct assay:GO:0001002 RNA
polymerase III type 1 promoter sequence-specific DNA
binding [PMID:2649882|PMID:2404611]"
/experiment="EXISTENCE:direct assay:GO:0001003 RNA
polymerase III type 2 promoter sequence-specific DNA
binding [PMID:2404611]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0006383
transcription by RNA polymerase III
[PMID:8464480|PMID:3633923|PMID:2183033]"
/experiment="EXISTENCE:direct assay:GO:0008301 DNA
binding, bending [PMID:2002052]"
/experiment="EXISTENCE:direct assay:GO:0042791 5S class
rRNA transcription by RNA polymerase III [PMID:2183033]"
/experiment="EXISTENCE:mutant phenotype:GO:0042791 5S
class rRNA transcription by RNA polymerase III
[PMID:8083243]"
/experiment="EXISTENCE:mutant phenotype:GO:0071168 protein
localization to chromatin [PMID:18708580]"
/note="Subunit of RNA polymerase III transcription
initiation factor complex; part of TauB domain of TFIIIC
that binds DNA at BoxB promoter sites of tRNA and similar
genes; cooperates with Tfc6p in DNA binding; largest of
six subunits of RNA polymerase III transcription
initiation factor complex (TFIIIC); colocalizes with
condensin at pol III genes and several ETC (extra TFIIIC)
sites; may have a role in recruiting or stabilizing Scc2/4
and condensin on chromosomes"
/codon_start=1
/product="transcription factor TFIIIC subunit TFC3"
/protein_id="NP_009400.1"
/db_xref="GeneID:851262"
/db_xref="SGD:S000000001"
/translation="MVLTIYPDELVQIVSDKIASNKGKITLNQLWDISGKYFDLSDKK
VKQFVLSCVILKKDIEVYCDGAITTKNVTDIIGDANHSYSVGITEDSLWTLLTGYTKK
ESTIGNSAFELLLEVAKSGEKGINTMDLAQVTGQDPRSVTGRIKKINHLLTSSQLIYK
GHVVKQLKLKKFSHDGVDSNPYINIRDHLATIVEVVKRSKNGIRQIIDLKRELKFDKE
KRLSKAFIAAIAWLDEKEYLKKVLVVSPKNPAIKIRCVKYVKDIPDSKGSPSFEYDSN
SADEDSVSDSKAAFEDEDLVEGLDNFNATDLLQNQGLVMEEKEDAVKNEVLLNRFYPL
QNQTYDIADKSGLKGISTMDVVNRITGKEFQRAFTKSSEYYLESVDKQKENTGGYRLF
RIYDFEGKKKFFRLFTAQNFQKLTNAEDEISVPKGFDELGKSRTDLKTLNEDNFVALN
NTVRFTTDSDGQDIFFWHGELKIPPNSKKTPNKNKRKRQVKNSTNASVAGNISNPKRI
KLEQHVSTAQEPKSAEDSPSSNGGTVVKGKVVNFGGFSARSLRSLQRQRAILKVMNTI
GGVAYLREQFYESVSKYMGSTTTLDKKTVRGDVDLMVESEKLGARTEPVSGRKIIFLP
TVGEDAIQRYILKEKDSKKATFTDVIHDTEIYFFDQTEKNRFHRGKKSVERIRKFQNR
QKNAKIKASDDAISKKSTSVNVSDGKIKRRDKKVSAGRTTVVVENTKEDKTVYHAGTK
DGVQALIRAVVVTKSIKNEIMWDKITKLFPNNSLDNLKKKWTARRVRMGHSGWRAYVD
KWKKMLVLAIKSEKISLRDVEELDLIKLLDIWTSFDEKEIKRPLFLYKNYEENRKKFT
LVRDDTLTHSGNDLAMSSMIQREISSLKKTYTRKISASTKDLSKSQSDDYIRTVIRSI
LIESPSTTRNEIEALKNVGNESIDNVIMDMAKEKQIYLHGSKLECTDTLPDILENRGN
YKDFGVAFQYRCKVNELLEAGNAIVINQEPSDISSWVLIDLISGELLNMDVIPMVRNV
RPLTYTSRRFEIRTLTPPLIIYANSQTKLNTARKSAVKVPLGKPFSRLWVNGSGSIRP
NIWKQVVTMVVNEIIFHPGITLSRLQSRCREVLSLHEISEICKWLLERQVLITTDFDG
YWVNHNWYSIYEST"
centromere 151465..151582
/note="CEN1; Chromosome I centromere"
/db_xref="SGD:S000006463"
centromere 151465..151474
/note="CEN1_CDEI of CEN1"
centromere 151475..151557
/note="CEN1_CDEII of CEN1"
centromere 151558..151582
/note="CEN1_CDEIII of CEN1"
gene <152257..>153876
/gene="NUP60"
/locus_tag="YAR002W"
/db_xref="GeneID:851263"
mRNA <152257..>153876
/gene="NUP60"
/locus_tag="YAR002W"
/product="FG-nucleoporin NUP60"
/transcript_id="NM_001178209.1"
/db_xref="GeneID:851263"
CDS 152257..153876
/gene="NUP60"
/locus_tag="YAR002W"
/experiment="EXISTENCE:direct assay:GO:0005543
phospholipid binding [PMID:17897934]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005643 nuclear
pore [PMID:10684247]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0044613 nuclear
pore central transport channel [PMID:18046406]"
/experiment="EXISTENCE:direct assay:GO:0044615 nuclear
pore nuclear basket [PMID:10684247]"
/experiment="EXISTENCE:genetic interaction:GO:0006607
NLS-bearing protein import into nucleus [PMID:15039779]"
/experiment="EXISTENCE:genetic interaction:GO:0017056
structural constituent of nuclear pore [PMID:11387327]"
/experiment="EXISTENCE:genetic interaction:GO:0060188
regulation of protein desumoylation [PMID:17403926]"
/experiment="EXISTENCE:mutant phenotype:GO:0000972
transcription-dependent tethering of RNA polymerase II
gene DNA at nuclear periphery
[PMID:20098417|PMID:21036941|PMID:20932479]"
/experiment="EXISTENCE:mutant phenotype:GO:0000973
post-transcriptional tethering of RNA polymerase II gene
DNA at nuclear periphery [PMID:20932479]"
/experiment="EXISTENCE:mutant phenotype:GO:0006302
double-strand break repair [PMID:17538013]"
/experiment="EXISTENCE:mutant phenotype:GO:0006607
NLS-bearing protein import into nucleus [PMID:11535617]"
/experiment="EXISTENCE:mutant phenotype:GO:0006611 protein
export from nucleus [PMID:11535617]"
/experiment="EXISTENCE:mutant phenotype:GO:0006913
nucleocytoplasmic transport [PMID:11535617]"
/experiment="EXISTENCE:mutant phenotype:GO:0008298
intracellular mRNA localization [PMID:21036941]"
/experiment="EXISTENCE:mutant phenotype:GO:0016973
poly(A)+ mRNA export from nucleus
[PMID:12411502|PMID:21036941]"
/experiment="EXISTENCE:mutant phenotype:GO:0017056
structural constituent of nuclear pore [PMID:17418788]"
/experiment="EXISTENCE:mutant phenotype:GO:0030466 silent
mating-type cassette heterochromatin formation
[PMID:21818277]"
/experiment="EXISTENCE:mutant phenotype:GO:0031990 mRNA
export from nucleus in response to heat stress
[PMID:18258809]"
/experiment="EXISTENCE:mutant phenotype:GO:0034398
telomere tethering at nuclear periphery
[PMID:11862215|PMID:21818277]"
/experiment="EXISTENCE:mutant phenotype:GO:0051276
chromosome organization [PMID:21818277]"
/experiment="EXISTENCE:physical interaction:GO:0017056
structural constituent of nuclear pore [PMID:17418788]"
/note="FG-nucleoporin component of the nuclear pore
complex central core; direct role in nucleocytoplasmic
transport and maintenance of the NPC permeability barrier;
role in gene tethering at the nuclear periphery; short
linear motifs connect Mlp1p and the Y-complex via Nup85p
to facilitate nuclear basket docking onto the core of the
NPC; Cdc5p-mediated phosphorylation leads to detachment
and reattachment of the basket during meiosis; both NUP1
and NUP60 are human NUP153 homologs"
/codon_start=1
/product="FG-nucleoporin NUP60"
/protein_id="NP_009401.1"
/db_xref="GeneID:851263"
/db_xref="SGD:S000000063"
/translation="MHRKSLRRASATVPSAPYRKQIISNAHNKPSLFSKIKTFFTQKD
SARVSPRNNVANKQPRNESFNRRISSMPGGYFHSEISPDSTVNRSVVVSAVGEARNDI
ENKEEEYDETHETNISNAKLANFFSKKGNEPLSEIEIEGVMSLLQKSSKSMITSEGEQ
KSAEGNNIDQSLILKESGSTPISISNAPTFNPKYDTSNASMNTTLGSIGSRKYSFNYS
SLPSPYKTTVYRYSAAKKIPDTYTANTSAQSIASAKSVRSGVSKSAPSKKISNTAAAL
VSLLDENDSKKNNAASELANPYSSYVSQIRKHKRVSPNAAPRQEISEEETTVKPLFQN
VPEQGEEPMKQLNATKISPSAPSKDSFTKYKPARSSSLRSNVVVAETSPEKKDGGDKP
PSSAFNFSFNTSRNVEPTENAYKSENAPSASSKEFNFTNLQAKPLVGKPKTELTKGDS
TPVQPDLSVTPQKSSSKGFVFNSVQKKSRSNLSQENDNEGKHISASIDNDFSEEKAEE
FDFNVPVVSKQLGNGLVDENKVEAFKSLYTF"
gene complement(<154065..>154724)
/gene="ERP1"
/locus_tag="YAR002C-A"
/db_xref="GeneID:851264"
mRNA complement(<154065..>154724)
/gene="ERP1"
/locus_tag="YAR002C-A"
/product="Erp1p"
/transcript_id="NM_001180033.1"
/db_xref="GeneID:851264"
CDS complement(154065..154724)
/gene="ERP1"
/locus_tag="YAR002C-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:14576278|PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0030134
COPII-coated ER to Golgi transport vesicle
[PMID:11157978]"
/experiment="EXISTENCE:mutant phenotype:GO:0006621 protein
retention in ER lumen [PMID:10359606]"
/experiment="EXISTENCE:mutant phenotype:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:10359606]"
/experiment="EXISTENCE:physical interaction:GO:0006888
endoplasmic reticulum to Golgi vesicle-mediated transport
[PMID:11157978]"
/note="Member of the p24 family involved in ER to Golgi
transport; role in misfolded protein quality control;
forms heterotrimeric complex with Erp2p, Emp24p, and
Erv25p; localized to COPII-coated vesicles; ERP1 has a
paralog, ERP6, that arose from the whole genome
duplication"
/codon_start=1
/product="Erp1p"
/protein_id="NP_009402.1"
/db_xref="GeneID:851264"
/db_xref="SGD:S000002129"
/translation="MLLTSLLQVFACCLVLPAQVTAFYYYTSGAERKCFHKELSKGTL
FQATYKAQIYDDQLQNYRDAGAQDFGVLIDIEETFDDNHLVVHQKGSASGDLTFLASD
SGEHKICIQPEAGGWLIKAKTKIDVEFQVGSDEKLDSKGKATIDILHAKVNVLNSKIG
EIRREQKLMRDREATFRDASEAVNSRAMWWIVIQLIVLAVTCGWQMKHLGKFFVKQKI
L"
gene <155005..>156285
/gene="SWD1"
/locus_tag="YAR003W"
/gene_synonym="CPS50; FUN16; SAF49"
/db_xref="GeneID:851265"
mRNA <155005..>156285
/gene="SWD1"
/locus_tag="YAR003W"
/gene_synonym="CPS50; FUN16; SAF49"
/product="COMPASS subunit protein SWD1"
/transcript_id="NM_001178210.1"
/db_xref="GeneID:851265"
CDS 155005..156285
/gene="SWD1"
/locus_tag="YAR003W"
/gene_synonym="CPS50; FUN16; SAF49"
/experiment="EXISTENCE:direct assay:GO:0042800 histone
H3K4 methyltransferase activity
[PMID:11805083|PMID:11742990]"
/experiment="EXISTENCE:mutant phenotype:GO:0000723
telomere maintenance [PMID:11742990]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:11687631]"
/experiment="EXISTENCE:mutant phenotype:GO:0031509
subtelomeric heterochromatin formation
[PMID:11752412|PMID:11805083|PMID:11687631]"
/experiment="EXISTENCE:mutant phenotype:GO:0042800 histone
H3K4 methyltransferase activity
[PMID:11752412|PMID:11805083]"
/experiment="EXISTENCE:physical interaction:GO:0048188
Set1C/COMPASS complex
[PMID:11742990|PMID:11687631|PMID:11752412]"
/note="Subunit of the COMPASS (Set1C) complex; COMPASS
methylates histone H3 on lysine 4 and is required in
transcriptional silencing near telomeres; WD40 beta
propeller superfamily member with similarity to mammalian
Rbbp7"
/codon_start=1
/product="COMPASS subunit protein SWD1"
/protein_id="NP_009403.1"
/db_xref="GeneID:851265"
/db_xref="SGD:S000000064"
/translation="MNILLQDPFAVLKEHPEKLTHTIENPLRTECLQFSPCGDYLALG
CANGALVIYDMDTFRPICVPGNMLGAHVRPITSIAWSPDGRLLLTSSRDWSIKLWDLS
KPSKPLKEIRFDSPIWGCQWLDAKRRLCVATIFEESDAYVIDFSNDPVASLLSKSDEK
QLSSTPDHGYVLVCTVHTKHPNIIIVGTSKGWLDFYKFHSLYQTECIHSLKITSSNIK
HLIVSQNGERLAINCSDRTIRQYEISIDDENSAVELTLEHKYQDVINKLQWNCILFSN
NTAEYLVASTHGSSAHELYIWETTSGTLVRVLEGAEEELIDINWDFYSMSIVSNGFES
GNVYVWSVVIPPKWSALAPDFEEVEENVDYLEKEDEFDEVDEAEQQQGLEQEEEIAID
LRTREQYDVRGNNLLVERFTIPTDYTRIIKMQSS"
gene complement(<156754..>158619)
/gene="RFA1"
/locus_tag="YAR007C"
/gene_synonym="BUF2; FUN3; RPA1; RPA70"
/db_xref="GeneID:851266"
mRNA complement(<156754..>158619)
/gene="RFA1"
/locus_tag="YAR007C"
/gene_synonym="BUF2; FUN3; RPA1; RPA70"
/product="replication factor A subunit protein RFA1"
/transcript_id="NM_001178211.1"
/db_xref="GeneID:851266"
CDS complement(156754..158619)
/gene="RFA1"
/locus_tag="YAR007C"
/gene_synonym="BUF2; FUN3; RPA1; RPA70"
/experiment="EXISTENCE:direct assay:GO:0000794 condensed
nuclear chromosome [PMID:9679065]"
/experiment="EXISTENCE:direct assay:GO:0003690
double-stranded DNA binding [PMID:7761422]"
/experiment="EXISTENCE:direct assay:GO:0003697
single-stranded DNA binding [PMID:8804316]"
/experiment="EXISTENCE:direct assay:GO:0003729 mRNA
binding [PMID:23222640]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0005662 DNA
replication factor A complex [PMID:2554144]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:15282802]"
/experiment="EXISTENCE:direct assay:GO:0005829 cytosol
[PMID:22932476]"
/experiment="EXISTENCE:direct assay:GO:0006260 DNA
replication [PMID:2554144]"
/experiment="EXISTENCE:direct assay:GO:0006265 DNA
topological change [PMID:22885009]"
/experiment="EXISTENCE:direct assay:GO:0006289
nucleotide-excision repair [PMID:8910442]"
/experiment="EXISTENCE:direct assay:GO:0030491
heteroduplex formation [PMID:12226081]"
/experiment="EXISTENCE:direct assay:GO:0043565
sequence-specific DNA binding [PMID:7761422]"
/experiment="EXISTENCE:genetic interaction:GO:0000722
telomere maintenance via recombination [PMID:17202155]"
/experiment="EXISTENCE:genetic interaction:GO:0000724
double-strand break repair via homologous recombination
[PMID:9679065]"
/experiment="EXISTENCE:mutant phenotype:GO:0000781
chromosome, telomeric region [PMID:14702040]"
/experiment="EXISTENCE:mutant phenotype:GO:0006260 DNA
replication [PMID:7969128]"
/experiment="EXISTENCE:mutant phenotype:GO:0006281 DNA
repair [PMID:7969128]"
/experiment="EXISTENCE:mutant phenotype:GO:0006312 mitotic
recombination [PMID:7969128]"
/experiment="EXISTENCE:mutant phenotype:GO:0007131
reciprocal meiotic recombination [PMID:12072452]"
/experiment="EXISTENCE:mutant phenotype:GO:0010833
telomere maintenance via telomere lengthening
[PMID:22354040]"
/experiment="EXISTENCE:mutant phenotype:GO:0016567 protein
ubiquitination [PMID:18342608]"
/experiment="EXISTENCE:physical interaction:GO:0007004
telomere maintenance via telomerase [PMID:2554144]"
/experiment="EXISTENCE:physical interaction:GO:0016567
protein ubiquitination [PMID:18342608]"
/experiment="EXISTENCE:physical interaction:GO:0045184
establishment of protein localization [PMID:2554144]"
/note="Subunit of heterotrimeric Replication Protein A
(RPA); RPA is a highly conserved ssDNA binding protein
involved in DNA replication, repair, and recombination;
RPA protects against inappropriate telomere recombination,
and upon telomere uncapping, prevents cell proliferation
by a checkpoint-independent pathway; promotes recruitment,
helicase and nuclease activities of Dna2p; role in DNA
catenation/decatenation pathway of chromosome
disentangling; relocalizes to the cytosol in response to
hypoxia"
/codon_start=1
/product="replication factor A subunit protein RFA1"
/protein_id="NP_009404.1"
/db_xref="GeneID:851266"
/db_xref="SGD:S000000065"
/translation="MSSVQLSRGDFHSIFTNKQRYDNPTGGVYQVYNTRKSDGANSNR
KNLIMISDGIYHMKALLRNQAASKFQSMELQRGDIIRVIIAEPAIVRERKKYVLLVDD
FELVQSRADMVNQTSTFLDNYFSEHPNETLKDEDITDSGNVANQTNASNAGVPDMLHS
NSNLNANERKFANENPNSQKTRPIFAIEQLSPYQNVWTIKARVSYKGEIKTWHNQRGD
GKLFNVNFLDTSGEIRATAFNDFATKFNEILQEGKVYYVSKAKLQPAKPQFTNLTHPY
ELNLDRDTVIEECFDESNVPKTHFNFIKLDAIQNQEVNSNVDVLGIIQTINPHFELTS
RAGKKFDRRDITIVDDSGFSISVGLWNQQALDFNLPEGSVAAIKGVRVTDFGGKSLSM
GFSSTLIPNPEIPEAYALKGWYDSKGRNANFITLKQEPGMGGQSAASLTKFIAQRITI
ARAQAENLGRSEKGDFFSVKAAISFLKVDNFAYPACSNENCNKKVLEQPDGTWRCEKC
DTNNARPNWRYILTISIIDETNQLWLTLFDDQAKQLLGVDANTLMSLKEEDPNEFTKI
TQSIQMNEYDFRIRAREDTYNDQSRIRYTVANLHSLNYRAEADYLADELSKALLA"
gene <158966..>159793
/gene="SEN34"
/locus_tag="YAR008W"
/gene_synonym="FUN4"
/db_xref="GeneID:851267"
mRNA <158966..>159793
/gene="SEN34"
/locus_tag="YAR008W"
/gene_synonym="FUN4"
/product="tRNA splicing endonuclease subunit SEN34"
/transcript_id="NM_001178212.1"
/db_xref="GeneID:851267"
CDS 158966..159793
/gene="SEN34"
/locus_tag="YAR008W"
/gene_synonym="FUN4"
/EC_number="4.6.1.16"
/experiment="EXISTENCE:direct assay:GO:0000213 tRNA-intron
lyase activity [PMID:9200603]"
/experiment="EXISTENCE:direct assay:GO:0000214 tRNA-intron
endonuclease complex [PMID:9200603]"
/experiment="EXISTENCE:direct assay:GO:0000379 tRNA-type
intron splice site recognition and cleavage
[PMID:9200603]"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:16823961]"
/experiment="EXISTENCE:direct assay:GO:0005741
mitochondrial outer membrane [PMID:16407407]"
/experiment="EXISTENCE:mutant phenotype:GO:0000213
tRNA-intron lyase activity [PMID:9200603]"
/experiment="EXISTENCE:mutant phenotype:GO:0000379
tRNA-type intron splice site recognition and cleavage
[PMID:9200603]"
/note="Subunit of the tRNA splicing endonuclease; tRNA
splicing endonuclease (Sen complex) is composed of Sen2p,
Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the
CBP1 mRNA at the mitochondrial surface; Sen34p contains
the active site for tRNA 3' splice site cleavage and has
similarity to Sen2p and to Archaeal tRNA splicing
endonuclease"
/codon_start=1
/product="tRNA splicing endonuclease subunit SEN34"
/protein_id="NP_009405.1"
/db_xref="GeneID:851267"
/db_xref="SGD:S000000066"
/translation="MPPLVFDIDHIKLLRKWGICGVLSGTLPTAAQQNVFLSVPLRLM
LEDVLWLHLNNLADVKLIRQEGDEIMEGITLERGAKLSKIVNDRLNKSFEYQRKFKKD
EHIAKLKKIGRINDKTTAEELQRLDKSSNNDQLIESSLFIDIANTSMILRDIRSDSDS
LSRDDISDLLFKQYRQAGKMQTYFLYKALRDQGYVLSPGGRFGGKFIAYPGDPLRFHS
HLTIQDAIDYHNEPIDLISMISGARLGTTVKKLWVIGGVAEETKETHFFSIEWAGFG"
rep_origin 159907..160127
/note="ARS101; ARS containing multiple redundant ORC
binding sites; ORC is the acronym for origin recognition
complex"
/db_xref="SGD:S000077372"
repeat_region complement(160105..160237)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006788"
mobile_element complement(160238..166162)
/note="YARCTy1-1; Ty1 element, LTR retrotransposon of the
Copia (Pseudoviridae) group; contains genes TYA Gag and
TYB Pol, encoding proteins involved in structure and
function of virus-like particles, flanked by two direct
repeats; mutated in S288C"
/mobile_element_type="retrotransposon:YARCTy1-1"
/db_xref="SGD:S000006792"
repeat_region complement(160238..160574)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006789"
gene complement(<160597..>164187)
/locus_tag="YAR009C"
/db_xref="GeneID:851268"
mRNA complement(<160597..>164187)
/locus_tag="YAR009C"
/product="truncated gag-pol fusion protein"
/transcript_id="NM_001178213.1"
/db_xref="GeneID:851268"
CDS complement(160597..164187)
/locus_tag="YAR009C"
/EC_number="2.7.7.7"
/EC_number="2.7.7.49"
/EC_number="3.1.26.4"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:9448009]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/note="Retrotransposon TYA Gag and TYB Pol genes; Gag
processing produces capsid proteins, Pol is cleaved to
produce protease, reverse transcriptase and integrase
activities; in YARCTy1-1 TYB is mutant and probably
non-functional; protein product forms cytoplasmic foci
upon DNA replication stress"
/codon_start=1
/product="truncated gag-pol fusion protein"
/protein_id="NP_009406.1"
/db_xref="GeneID:851268"
/db_xref="SGD:S000000067"
/translation="METFTGYLKSTCFHQISPYPPSIMSIQVKVHANILILSFIECLR
MPMHRQIRYSLKNNTITYFNESDVDWSSAIDYQCPDCLIGKSTKHRHIKGSRLKYQNS
YEPFQYLHTDIFGPVHNLPKSAPSYFISFTDETTKLRWVYPLHDRREDSILDVFTTIL
AFIKNQFQASVLVIQMDRGSEYTNRTLHKFLEKNGITPCYTTTADSRAHGVAERLNRT
LLDDCRTQLQCSGLPNHLWFSAIEFSTIVRNSLASPKSKKSARQHAGLAGLDISTLLP
FGQPVIVNDHNPNSKIHPRGIPGYALHPSRNSYGYIIYLPSLKKTVDTTNYVILQGKE
SRLDQFNYDALTFDEDLNRLTASYHSFIASNEIQESNDLNIESDHDFQSDIELHPEQP
RNVLSKAVSPTDSTPPSTHTEDSKRVSKTNIRAPREVDPNISESNILPSKKRSSTPQI
SNIESTGSGGMHKLNVPLLAPMSQSNTHESSHASKSKDFRHSDSYSENETNHTNVPIS
STGGTNNKTVPQISDQETEKRIIHRSPSIDASPPENNSSHNIVPIKTPTTVSEQNTEE
SIIADLPLPDLPPESPTEFPDPFKELPPINSHQTNSSLGGIGDSNAYTTINSKKRSLE
DNETEIKVSRDTWNTKNMRSLEPPRSKKRIHLIAAVKAVKSIKPIRTTLRYDEAITYN
KDIKEKEKYIEAYHKEVNQLLKMNTWDTDKYYDRKEIDPKRVINSMFIFNKKRDGTHK
ARFVARGDIQHPDTYDTGMQSNTVHHYALMTSLSLALDNNYYITQLDISSAYLYADIK
EELYIRPPPHLGMNDKLIRLKKSLYGLKQSGANWYETIKSYLIKQCGMEEVRGWSCVF
KNSQVTICLFVDDMILFSKDLNANKKIITTLKKQYDTKIINLGESDNEIQYDILGLEI
KYQRGKYMKLGMEKSLTEKLPKLNVPLNPKGKKLRAPGQPGLYIDQDELEIDEDEYKE
KVHEMQKLIGLASYVGYKFRFDLLYYINTLAQHILFPSRQVLDMTYELIQFMWDTRDK
QLIWHKNKPTEPDNKLVAISDASYGNQPYYKSQIGNIYLLNGKVIGGKSTKASLTCTS
TTEAEIHAISESVPLLNNLSYLIQELNKKPIIKGLLTDSRSTISIIKSTNEEKFRNRF
FGTKAMRLRDEVSGNNLYVYYIETKKNIADVMTKPLPIKTFKLLTNKWIH"
gene complement(<164544..>165866)
/locus_tag="YAR010C"
/db_xref="GeneID:851269"
mRNA complement(<164544..>165866)
/locus_tag="YAR010C"
/product="gag protein"
/transcript_id="NM_001178214.1"
/db_xref="GeneID:851269"
CDS complement(164544..165866)
/locus_tag="YAR010C"
/note="Retrotransposon TYA Gag gene co-transcribed with
TYB Pol; Gag processing produces capsid proteins; in
YARCTy1-1 TYB is mutant and probably non-functional"
/codon_start=1
/product="gag protein"
/protein_id="NP_009407.1"
/db_xref="GeneID:851269"
/db_xref="SGD:S000000068"
/translation="MESQQLSQHSPISHGSACASVTSKEVHTNQDPLDVSASKTEECE
KASTKANSQQTTTPASSAVPENPHHASPQTAQSHSPQNGPYPQQCMMTQNQANPSGWS
FYGHPSMIPYTPYQMSPMYFPPGPQSQFPQYPSSVGTPLSTPSPESGNTFTDSSSADS
DMTSTKKYVRPPPMLTSPNDFPNWVKTYIKFLQNSNLGGIIPTVNGKPVRQITDDELT
FLYNTFQIFAPSQFLPTWVKDILSVDYTDIMKILSKSIEKMQSDTQEANDIVTLANLQ
YNGSTPADAFETKVTNIIDRLNNNGIHINNKVACQLIMRGLSGEYKFLRYTRHRHLNM
TVAELFLDIHAIYEEQQGSRNSKPNYRRNPSDEKNDSRSYTNTTKPKVIARNPQKTNN
SKSKTARAHNVSTSNNSPSTDNDSISKSTTEPIQLNNKHDLHLRPETY"
repeat_region complement(165826..166162)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006790"
gene 166267..166339
/gene="TGA1"
/locus_tag="YNCA0004W"
/db_xref="GeneID:851270"
tRNA 166267..166339
/gene="TGA1"
/locus_tag="YNCA0004W"
/product="tRNA-Ala"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Alanine tRNA (tRNA-Ala), predicted by tRNAscan-SE
analysis; one of 5 nuclear tRNA genes containing the
tDNA-anticodon TGC (mature tRNA may be UGC or may contain
modified bases), decodes GCA and probably GCG codons into
alanine, one of 16 nuclear tRNAs for alanine"
/db_xref="GeneID:851270"
/db_xref="SGD:S000006521"
gene complement(<166742..>168871)
/gene="BUD14"
/locus_tag="YAR014C"
/db_xref="GeneID:851271"
mRNA complement(<166742..>168871)
/gene="BUD14"
/locus_tag="YAR014C"
/product="protein phosphatase regulator BUD14"
/transcript_id="NM_001178215.2"
/db_xref="GeneID:851271"
CDS complement(166742..168871)
/gene="BUD14"
/locus_tag="YAR014C"
/experiment="EXISTENCE:direct assay:GO:0000131 incipient
cellular bud site [PMID:11452010]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005934 cellular
bud tip [PMID:11452010]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:11452010]"
/experiment="EXISTENCE:direct assay:GO:0006355 regulation
of DNA-templated transcription [PMID:15601868]"
/experiment="EXISTENCE:direct assay:GO:0019888 protein
phosphatase regulator activity [PMID:16107882]"
/experiment="EXISTENCE:direct assay:GO:0030837 negative
regulation of actin filament polymerization
[PMID:19217430]"
/experiment="EXISTENCE:direct assay:GO:0032880 regulation
of protein localization [PMID:19217430]"
/experiment="EXISTENCE:direct assay:GO:1990615
Kelch-containing formin regulatory complex
[PMID:24828508]"
/experiment="EXISTENCE:genetic interaction:GO:0008360
regulation of cell shape [PMID:12477789]"
/experiment="EXISTENCE:genetic interaction:GO:0030837
negative regulation of actin filament polymerization
[PMID:19217430]"
/experiment="EXISTENCE:genetic interaction:GO:0032465
regulation of cytokinesis [PMID:24828508]"
/experiment="EXISTENCE:genetic interaction:GO:0090337
regulation of formin-nucleated actin cable assembly
[PMID:24828508]"
/experiment="EXISTENCE:mutant phenotype:GO:0001100
negative regulation of exit from mitosis [PMID:34633288]"
/experiment="EXISTENCE:mutant phenotype:GO:0006355
regulation of DNA-templated transcription [PMID:15601868]"
/experiment="EXISTENCE:mutant phenotype:GO:0007010
cytoskeleton organization [PMID:16107882]"
/experiment="EXISTENCE:mutant phenotype:GO:0019888 protein
phosphatase regulator activity [PMID:16107882]"
/experiment="EXISTENCE:mutant phenotype:GO:0030837
negative regulation of actin filament polymerization
[PMID:19217430]"
/experiment="EXISTENCE:mutant phenotype:GO:0060627
regulation of vesicle-mediated transport [PMID:24828508]"
/experiment="EXISTENCE:mutant phenotype:GO:1905047 mitotic
spindle pole body organization [PMID:39296336]"
/note="Protein phosphatase regulator; subunit of the
Bud14p-Glc7p complex that localizes Glc7p to the cell
cortex where it activates the dynein/dynactin complex;
subunit of the KFRC complex with Kel1p and Kel2p that
regulates Bnr1p (formin) to control actin cable assembly,
cytokinesis, and polarized growth; involved in spindle
pole body size maintenance; involved in bud-site
selection; relative distribution to the nucleus increases
upon DNA replication stress"
/codon_start=1
/product="protein phosphatase regulator BUD14"
/protein_id="NP_009408.3"
/db_xref="GeneID:851271"
/db_xref="SGD:S000000069"
/translation="MSNKEEHVDETSASGVKEVSSIAARHDNGYAPSLITSTSGMDSF
QSHALLNDPTLIEDYSDIINNRPTSGSKLTLGNEDSESMGGSVVVTPTSNKSSPFNSK
LNILSNAAEKGHDVLRNRDDDKELEEENVEKHMHSNSKRDQRHYKENSSELPDSYDYS
DSEFEDNLERRLQEIETDSVDSADKDEVHFSVNNTMNPDVDDFSDGLKYAISEDEDEE
ENYSDDDDFDRKFQDSGFQGEKDDLEEENDDYQPLSPPRELDPDKLYALYAFNGHDSS
HCQLGQDEPCILLNDQDAYWWLVKRITDGKIGFAPAEILETFPERLARLNCWKNENMS
SQSVASSDSKDDSISSGNKNQSDAESIIPTPALNGYGKGNKSVSFNDVVGYADRFIDD
AIEDTSLDSNDDGGEGNGQSYDDDVDNDKETKVTHRDEYTEAKLNFGKFQDDDTSDVV
SDVSFSTSLNTPLNVKKVRRQDNKNESEPKTSSSKDREDDYNANRYVGQEKSEPVDSD
YDTDLKKVFEAPRMPFANGMAKSDSQNSLSTIGEFSPSSSEWTNESPSTPIVEESSSI
PSSRAIKDISQYIHAKSKIEETTNVENTEGQIQASLGSSGGMANQTDAEQPKEELEKH
HSTPEEEKQSTLSLHSSSEEDFYMDEQRAVSSASINSSLSGSRALSNTNMSDPASKPN
SLVQHLYAPVFDRMDVLMKQLDEIIRK"
gene <169375..>170295
/gene="ADE1"
/locus_tag="YAR015W"
/db_xref="GeneID:851272"
mRNA <169375..>170295
/gene="ADE1"
/locus_tag="YAR015W"
/product="phosphoribosylaminoimidazolesuccinocarboxamide
synthase"
/transcript_id="NM_001178216.1"
/db_xref="GeneID:851272"
CDS 169375..170295
/gene="ADE1"
/locus_tag="YAR015W"
/EC_number="6.3.2.6"
/experiment="EXISTENCE:direct assay:GO:0004639
phosphoribosylaminoimidazolesuccinocarboxamide synthase
activity [PMID:1756975]"
/experiment="EXISTENCE:direct assay:GO:0005634 nucleus
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:14562095]"
/experiment="EXISTENCE:direct assay:GO:0006164 purine
nucleotide biosynthetic process [PMID:1756975]"
/experiment="EXISTENCE:direct assay:GO:0006189 'de novo'
IMP biosynthetic process [PMID:1756975]"
/experiment="EXISTENCE:mutant phenotype:GO:0004639
phosphoribosylaminoimidazolesuccinocarboxamide synthase
activity [PMID:5767025|PMID:5767024]"
/experiment="EXISTENCE:mutant phenotype:GO:0006164 purine
nucleotide biosynthetic process
[PMID:5767024|PMID:5767025]"
/experiment="EXISTENCE:mutant phenotype:GO:0006189 'de
novo' IMP biosynthetic process
[PMID:5767024|PMID:5767025]"
/note="N-succinyl-5-aminoimidazole-4-carboxamide ribotide
synthetase; required for 'de novo' purine nucleotide
biosynthesis; red pigment accumulates in mutant cells
deprived of adenine; protein abundance increases in
response to DNA replication stress"
/codon_start=1
/product="phosphoribosylaminoimidazolesuccinocarboxamide
synthase"
/protein_id="NP_009409.1"
/db_xref="GeneID:851272"
/db_xref="SGD:S000000070"
/translation="MSITKTELDGILPLVARGKVRDIYEVDAGTLLFVATDRISAYDV
IMENSIPEKGILLTKLSEFWFKFLSNDVRNHLVDIAPGKTIFDYLPAKLSEPKYKTQL
EDRSLLVHKHKLIPLEVIVRGYITGSAWKEYVKTGTVHGLKQPQGLKESQEFPEPIFT
PSTKAEQGEHDENISPAQAAELVGEDLSRRVAELAVKLYSKCKDYAKEKGIIIADTKF
EFGIDEKTNEIILVDEVLTPDSSRFWNGASYKVGESQDSYDKQFLRDWLTANKLNGVN
GVKMPQDIVDRTRAKYIEAYETLTGSKWSH"
gene complement(<170396..>171703)
/gene="KIN3"
/locus_tag="YAR018C"
/gene_synonym="FUN52; NPK1"
/db_xref="GeneID:851273"
mRNA complement(<170396..>171703)
/gene="KIN3"
/locus_tag="YAR018C"
/gene_synonym="FUN52; NPK1"
/product="serine/threonine protein kinase KIN3"
/transcript_id="NM_001178217.1"
/db_xref="GeneID:851273"
CDS complement(170396..171703)
/gene="KIN3"
/locus_tag="YAR018C"
/gene_synonym="FUN52; NPK1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:mutant phenotype:GO:0007059
chromosome segregation [PMID:12386167]"
/note="Nonessential serine/threonine protein kinase;
possible role in DNA damage response; influences tolerance
to high levels of ethanol"
/codon_start=1
/product="serine/threonine protein kinase KIN3"
/protein_id="NP_009410.1"
/db_xref="GeneID:851273"
/db_xref="SGD:S000000071"
/translation="MHRRQFFQEYRSPQQQQGHPPRSEYQVLEEIGRGSFGSVRKVIH
IPTKKLLVRKDIKYGHMNSKERQQLIAECSILSQLKHENIVEFYNWDFDEQKEVLYLY
MEYCSRGDLSQMIKHYKQEHKYIPEKIVWGILAQLLTALYKCHYGVELPTLTTIYDRM
KPPVKGKNIVIHRDLKPGNIFLSYDDSDYNINEQVDGHEEVNSNYYRDHRVNSGKRGS
PMDYSQVVVKLGDFGLAKSLETSIQFATTYVGTPYYMSPEVLMDQPYSPLSDIWSLGC
VIFEMCSLHPPFQAKNYLELQTKIKNGKCDTVPEYYSRGLNAIIHSMIDVNLRTRPST
FELLQDIQIRTARKSLQLERFERKLLDYENELTNIEKILEKQAIEYERELSQLKEQFT
QAVEERAREVISGKKVGKVPESINGYYGKKFAKPAYHWQTRYR"
gene complement(<172211..>175135)
/gene="CDC15"
/locus_tag="YAR019C"
/gene_synonym="LYT1; RLT1"
/db_xref="GeneID:851274"
mRNA complement(<172211..>175135)
/gene="CDC15"
/locus_tag="YAR019C"
/gene_synonym="LYT1; RLT1"
/product="serine/threonine protein kinase CDC15"
/transcript_id="NM_001178218.2"
/db_xref="GeneID:851274"
CDS complement(172211..175135)
/gene="CDC15"
/locus_tag="YAR019C"
/gene_synonym="LYT1; RLT1"
/EC_number="2.7.11.1"
/experiment="EXISTENCE:direct assay:GO:0004672 protein
kinase activity [PMID:16319894]"
/experiment="EXISTENCE:direct assay:GO:0004674 protein
serine/threonine kinase activity
[PMID:23579499|PMID:11404483]"
/experiment="EXISTENCE:direct assay:GO:0005737 cytoplasm
[PMID:22842922]"
/experiment="EXISTENCE:direct assay:GO:0005816 spindle
pole body [PMID:10662594|PMID:12832486]"
/experiment="EXISTENCE:direct assay:GO:0005935 cellular
bud neck [PMID:12832486]"
/experiment="EXISTENCE:genetic interaction:GO:1903024
positive regulation of ascospore-type prospore membrane
formation [PMID:32788308]"
/experiment="EXISTENCE:mutant phenotype:GO:0000281 mitotic
cytokinesis [PMID:11267871]"
/experiment="EXISTENCE:mutant phenotype:GO:0004674 protein
serine/threonine kinase activity [PMID:23579499]"
/experiment="EXISTENCE:mutant phenotype:GO:0007096
regulation of exit from mitosis [PMID:11267871]"
/experiment="EXISTENCE:mutant phenotype:GO:0051229 meiotic
spindle disassembly [PMID:17660551|PMID:32788308]"
/experiment="EXISTENCE:mutant phenotype:GO:1903024
positive regulation of ascospore-type prospore membrane
formation [PMID:32788308]"
/experiment="EXISTENCE:mutant phenotype:GO:1904750
negative regulation of protein localization to nucleolus
[PMID:32788308]"
/note="Hippo-like kinase of the Mitotic Exit Network;
promotes exit by activating the Dbf2p kinase; component of
a non-canonical Hippo pathway with Sps1p required for
prospore membrane closure, spindle disassembly and
sustained release of Cdc14p during meiotic anaphase II;
complexes with Sps1p and contributes to its
phosphorylation; phosphorylates the RNAPII CTD during
mitosis; localizes to the bud neck and SPB during anaphase
and telophase; relocalizes to the cytoplasm upon DNA
replication stress"
/codon_start=1
/product="serine/threonine protein kinase CDC15"
/protein_id="NP_009411.2"
/db_xref="GeneID:851274"
/db_xref="SGD:S000000072"
/translation="MNSMADTDRVNLTPIQRASEKSVQYHLKQVIGRGSYGVVYKAIN
KHTDQVVAIKEVVYENDEELNDIMAEISLLKNLNHNNIVKYHGFIRKSYELYILLEYC
ANGSLRRLISRSSTGLSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANILLSADNTV
KLADFGVSTIVNSSALTLAGTLNWMAPEILGNRGASTLSDIWSLGATVVEMLTKNPPY
HNLTDANIYYAVENDTYYPPSSFSEPLKDFLSKCFVKNMYKRPTADQLLKHVWINSTE
NVKVDKLNKFKEDFTDADYHWDADFQEEKLNISPSKFSLAAAPAAWAENNQELDLMPP
TESQLLSQLKSSSKPLTDLHVLFSVCSLENIADTIIECLSRTTVDKRLITAFGSIFVY
DTQHNHSRLRLKFIAMGGIPLIIKFEHLAKEFVIDYPQTLIECGIMYPPNFASLKTPK
YILELVYRFYDLTSTAFWCRWCFKHLDISLLLNNIHERRAQSILLKLSSYAPWSFEKI
LPSLIDSKLKKKILISPQITYVVFKSINYMITTNDDKIHKSAIPSSSSLPLSSSPTRN
SPVNSVQSPSRSPVHSLMATRPSSPMRHKSISNFPHLTISSKSRLLIELPEGFFTWLT
SFFVDMAQIKDLSVLKYFTKLCYLTVHINSTFLNDLLDNDAFFAFIRNIDTIIPFIDD
AKTAAFIWKQITAICVEMSLDMDQMSASLFSTAMNFIRKKNNTSISGLEIILNCLHFT
LRNVNDDVAPTVGSSESHSVFLIKVNNDAAIELPIDQLVDLFYALNDDDVNLSKLISI
FTKICSLPGFENLTINIIFHPNFYEKIVSFFDTYFNSLLIQIDLLKFIKLIFSKSLLK
LYDYTGQPDPIKQTEPNRRNKATVFKLRAILVQITEFLNNNWNKDVPKRNSNQVGGDS
VLICQLCEDIRSLSKKGSLQKVSSVTAAIGSSPTKDERSNLRSSKDKSDGFSVPITTF
QT"
rep_origin 176157..176404
/note="ARS110; Autonomously Replicating Sequence;
originally referred to as ADE1 ARS"
/db_xref="SGD:S000114488"
gene complement(<176856..>177023)
/gene="PAU7"
/locus_tag="YAR020C"
/db_xref="GeneID:851275"
mRNA complement(<176856..>177023)
/gene="PAU7"
/locus_tag="YAR020C"
/product="seripauperin PAU7"
/transcript_id="NM_001178219.1"
/db_xref="GeneID:851275"
CDS complement(176856..177023)
/gene="PAU7"
/locus_tag="YAR020C"
/note="Member of the seripauperin multigene family; active
during alcoholic fermentation, regulated by anaerobiosis,
inhibited by oxygen, repressed by heme"
/codon_start=1
/product="seripauperin PAU7"
/protein_id="NP_009412.1"
/db_xref="GeneID:851275"
/db_xref="SGD:S000000073"
/translation="MVKLTSIAAGVAAIAAGASAAATTTLSQSDERVNLVELGVYVSD
IRAHLAEYYSF"
gene complement(<179281..>179820)
/gene="DFP1"
/locus_tag="YAR023C"
/db_xref="GeneID:851276"
mRNA complement(<179281..>179820)
/gene="DFP1"
/locus_tag="YAR023C"
/product="DUP240 family protein"
/transcript_id="NM_001178220.2"
/db_xref="GeneID:851276"
CDS complement(179281..179820)
/gene="DFP1"
/locus_tag="YAR023C"
/note="Putative integral membrane protein; member of
DUP240 gene family"
/codon_start=1
/product="DUP240 family protein"
/protein_id="NP_009413.2"
/db_xref="GeneID:851276"
/db_xref="SGD:S000000074"
/translation="MINFLLFVLTILATLTNIWFSGVLSPAMVIRICLGGSMVVLQIW
SFSRPISNETFRTKLLLEVITHRPSIAGKEWKTITYNMNQYLFKAGLWKTPYHFFCEH
QCYEFFKDLIKGKYPDVQWDTANTQPFISVPENQAATQNSDVEPTVKWCLFKAAEIQA
HAVREYWQSQYPDVGIPAI"
gene 181141..181254
/gene="SUP56"
/locus_tag="YNCA0005W"
/db_xref="GeneID:851277"
tRNA join(181141..181178,181211..181254)
/gene="SUP56"
/locus_tag="YNCA0005W"
/product="tRNA-Leu"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/note="Leucine tRNA (tRNA-Leu), predicted by tRNAscan-SE
analysis; can mutate to suppress amber nonsense mutations"
/db_xref="GeneID:851277"
/db_xref="SGD:S000006636"
gene complement(182522..182603)
/locus_tag="YNCA0006C"
/db_xref="GeneID:851278"
tRNA complement(182522..182603)
/locus_tag="YNCA0006C"
/product="tRNA-Ser"
/experiment="EXISTENCE:curator inference:GO:0005829
cytosol [PMID:9023104]"
/experiment="EXISTENCE:curator inference:GO:0006414
translational elongation [PMID:9023104]"
/experiment="EXISTENCE:direct assay:GO:0030371 translation
repressor activity [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043022 ribosome
binding [PMID:27609601]"
/experiment="EXISTENCE:direct assay:GO:0043555 regulation
of translation in response to stress [PMID:27609601]"
/note="Serine tRNA (tRNA-Ser), predicted by tRNAscan-SE
analysis"
/db_xref="GeneID:851278"
/db_xref="SGD:S000006719"
repeat_region 182620..182959
/note="Ty3 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006795"
repeat_region 183142..183474
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006793"
gene <183770..>184477
/gene="UIP3"
/locus_tag="YAR027W"
/db_xref="GeneID:851279"
mRNA <183770..>184477
/gene="UIP3"
/locus_tag="YAR027W"
/product="DUP240 family protein UIP3"
/transcript_id="NM_001178221.1"
/db_xref="GeneID:851279"
CDS 183770..184477
/gene="UIP3"
/locus_tag="YAR027W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:11056382]"
/note="Putative integral membrane hypothetical protein;
interacts with Ulp1p at the nuclear periphery; member of
DUP240 gene family"
/codon_start=1
/product="DUP240 family protein UIP3"
/protein_id="NP_009414.1"
/db_xref="GeneID:851279"
/db_xref="SGD:S000000075"
/translation="MQTPSENTDVKLDTLDEPSAHLIEENVALPEDTFNSYWSYILNE
IARCKPLMIMFLIPVCLVLLITFFHDIKGILVFLVISLILSIIILLIGITAFVSETLN
KGFIIKLLVEVITRKPAVGGKEWRIIAYNMNQYLFDHGIWHTPYYFFCEHRCHKFFKS
LIKQTRSNAHLSSPTNGAENTQSNTPAKEVSNEMVKPYIFSSDPVLEAYLIKAAEIHK
EAEFEYWRKQYPEVDLP"
gene <184892..>185596
/gene="KTD1"
/locus_tag="YAR028W"
/db_xref="GeneID:851280"
mRNA <184892..>185596
/gene="KTD1"
/locus_tag="YAR028W"
/product="DUP240 family protein"
/transcript_id="NM_001178222.1"
/db_xref="GeneID:851280"
CDS 184892..185596
/gene="KTD1"
/locus_tag="YAR028W"
/experiment="EXISTENCE:direct assay:GO:0000329 fungal-type
vacuole membrane
[PMID:26928762|PMID:36800387|PMID:19001347]"
/experiment="EXISTENCE:direct assay:GO:0097237 cellular
response to toxic substance [PMID:36800387]"
/experiment="EXISTENCE:mutant phenotype:GO:0097237
cellular response to toxic substance [PMID:36800387]"
/note="Killer toxin defense factor; key polymorphic factor
in the defense against K28 toxin; localizes mainly to
vacuolar membrane; most likely acts against K28 during its
trafficking after endocytosis; member of DUP240 gene
family"
/codon_start=1
/product="DUP240 family protein"
/protein_id="NP_009415.1"
/db_xref="GeneID:851280"
/db_xref="SGD:S000000076"
/translation="MQTPSENTDVKMDTLDEPSAHLIEENVALPEDTFSSHLSYVLYE
IAHCKPIMFMIIIIVSLISLIVLFHDNDGCTVILVMSLIVASMALMVVAAFTFGKAIT
EQEFMIKLLVEVIARKPAGKEWGTVAYNMNQYLFMKRLWYTPYYFYSGKKCHEFFTTL
IKEVNSGSHSDSSSNSAEDTQSPVSAGKTSNGLNNFYSIRSDPILMAYVLKATQIEKE
AQSEYWRKQYPDADLP"
gene <186321..>186545
/gene="DFP2"
/locus_tag="YAR029W"
/db_xref="GeneID:851281"
mRNA <186321..>186545
/gene="DFP2"
/locus_tag="YAR029W"
/product="DUP240 family protein"
/transcript_id="NM_001178223.1"
/db_xref="GeneID:851281"
CDS 186321..186545
/gene="DFP2"
/locus_tag="YAR029W"
/experiment="EXISTENCE:mutant phenotype:GO:0005737
cytoplasm [PMID:12101299]"
/note="Member of DUP240 gene family but contains no
transmembrane domains; green fluorescent protein
(GFP)-fusion protein localizes to the cytoplasm in a
punctate pattern"
/codon_start=1
/product="DUP240 family protein"
/protein_id="NP_009416.1"
/db_xref="GeneID:851281"
/db_xref="SGD:S000000077"
/translation="MNKYLFDHKIWSTPYYFYCEEDCHRLFLSFIEGRTFEKPTSNAE
ENVQETEAGESFTLNPGEDFQNCFPRQRIL"
gene <186836..>187732
/gene="PRM9"
/locus_tag="YAR031W"
/db_xref="GeneID:851282"
mRNA <186836..>187732
/gene="PRM9"
/locus_tag="YAR031W"
/product="pheromone-regulated DUP240 family protein PRM9"
/transcript_id="NM_001178224.1"
/db_xref="GeneID:851282"
CDS 186836..187732
/gene="PRM9"
/locus_tag="YAR031W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762]"
/experiment="EXISTENCE:direct assay:GO:0005886 plasma
membrane [PMID:12101299]"
/experiment="EXISTENCE:physical interaction:GO:0005783
endoplasmic reticulum [PMID:12925749]"
/note="Pheromone-regulated protein; contains 3 predicted
transmembrane segments and an FF sequence, a motif
involved in COPII binding; member of DUP240 gene family;
PRM9 has a paralog, PRM8, that arose from a segmental
duplication"
/codon_start=1
/product="pheromone-regulated DUP240 family protein PRM9"
/protein_id="NP_009418.1"
/db_xref="GeneID:851282"
/db_xref="SGD:S000000078"
/translation="MSPQYHFYFVSFRNLVLNEKCLRSKKQVMKSFNWYKTDRYFDPH
NILQHHSRAIEKTRYKLGMQTSSESTDAKSDFLDEPSAYLIEKNVALPKDIFGSYLSY
WIYEVTRHKAAVILLVLIVTSILLLVFFYNTEFCVAFEILLFSFCFPGTCMVVIAFSE
PIGDREFKVKLLMEIITRKPAVKGKEWRTITYKMNQYLFDHGLWDTPYYFYRDEDCHR
YFLSLIKGRTFKKQKESSASNVKDAQSNDETAGTPNEAAESSSFSAGPNFIKLLTKAA
EIEQQFQKEYWRQEYPGVDEFF"
gene <188107..>188811
/gene="MST28"
/locus_tag="YAR033W"
/db_xref="GeneID:851284"
mRNA <188107..>188811
/gene="MST28"
/locus_tag="YAR033W"
/product="DUP240 family protein MST28"
/transcript_id="NM_001178225.1"
/db_xref="GeneID:851284"
CDS 188107..188811
/gene="MST28"
/locus_tag="YAR033W"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:26928762|PMID:12925749]"
/experiment="EXISTENCE:direct assay:GO:0005794 Golgi
apparatus [PMID:12925749]"
/experiment="EXISTENCE:direct assay:GO:0016020 membrane
[PMID:12925749]"
/experiment="EXISTENCE:genetic interaction:GO:0016050
vesicle organization [PMID:12925749]"
/experiment="EXISTENCE:mutant phenotype:GO:0005783
endoplasmic reticulum [PMID:12101299]"
/experiment="EXISTENCE:mutant phenotype:GO:0005886 plasma
membrane [PMID:12101299]"
/experiment="EXISTENCE:physical interaction:GO:0016050
vesicle organization [PMID:12925749]"
/note="Putative integral membrane protein, involved in
vesicle formation; forms complex with Mst27p; member of
DUP240 gene family; binds COPI and COPII vesicles; MST28
has a paralog, MST27, that arose from a segmental
duplication"
/codon_start=1
/product="DUP240 family protein MST28"
/protein_id="NP_009419.1"
/db_xref="GeneID:851284"
/db_xref="SGD:S000000079"
/translation="MQTPPESTDVKLDTLNEPSAHLIEKNVALPKDIFRSYLSYWIYE
IARYTPVMILSLVIGVLVLLIIFFNDNEACVFNSAIFAFTSLVGLLIILSDGNPKLVS
RRNFRTELLVDVITRKPAVEGKEWRIITYNMNQYLFNHGQWHTPYYFYSDEDCYRYFL
RLVEGVTPKKQTATSIGNSPVTAKPEDAIESASPSSRLNYQNFLLKAAEIERQAQENY
WRRRHPNIDALLKKTE"
repeat_region 189426..189757
/note="Ty2 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006794"
gene <190193..>192256
/gene="YAT1"
/locus_tag="YAR035W"
/db_xref="GeneID:851285"
mRNA <190193..>192256
/gene="YAT1"
/locus_tag="YAR035W"
/product="carnitine O-acetyltransferase YAT1"
/transcript_id="NM_001178226.1"
/db_xref="GeneID:851285"
CDS 190193..192256
/gene="YAT1"
/locus_tag="YAR035W"
/EC_number="2.3.1.7"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion
[PMID:14576278|PMID:11914276|PMID:16823961|PMID:18427809]"
/experiment="EXISTENCE:mutant phenotype:GO:0009437
carnitine metabolic process [PMID:11329169]"
/note="Outer mitochondrial carnitine acetyltransferase;
minor ethanol-inducible enzyme involved in transport of
activated acyl groups from the cytoplasm into the
mitochondrial matrix; phosphorylated"
/codon_start=1
/product="carnitine O-acetyltransferase YAT1"
/protein_id="NP_009420.1"
/db_xref="GeneID:851285"
/db_xref="SGD:S000000080"
/translation="MPNLKRLPIPPLQDTLNRYLARVEPLQDERQNRRTRRTVLSAEN
LDALNTLHERLLEYDARLAESNPESSYIEQFWYDAYLLYDATVVLNVNPYFQLQDDPT
IKDTPETAAQGPYGAHTVQVRRAARLTTSILKFIRQIRHGTLRTDTVRGKTPLSMDQY
ERLFGSSRIPPGPGEPSCHLQTDATSHHVVAMYRGQFYWFDVLDTRNEPIFATPEQLE
WNLYSIIMDAESAGSGSAPFGVFTTESRRVWSNIRDYLFHADDCTNWRNLKLIDSALF
VVCLDDVAFAADQQDELTRSMLCGTSTINLDPHQHQPPLNVQTGTCLNRWYDKLQLIV
TKNGKAGINFEHTGVDGHTVLRLATDIYTDSILSFARGVTKNVVDIFSDDDGKPSSSS
LASAAHSANLITIPRKLEWRTDNFLQSSLHFAETRISDLISQYEFVNLDFSNYGASHI
KTVFKCSPDAFVQQVFQVAYFALYGRFETVYEPAMTKAFQNGRTEAIRSVTGQSKLFV
KSLLDQDASDATKIQLLHDACTAHSQITRECSQGLGQDRHLYALYCLWNQWYKDKLEL
PPIFRDKSWTTMQNNVLSTSNCGNPCLKSFGFGPVTANGFGIGYIIRDHSVSVVVSSR
HRQTARFASLMEKSLLEIDRIFKRQQARAAKPAARTTASANTKSEDMKYLLSGYDYFD
VSVSG"
gene complement(<192337..>192417)
/locus_tag="YAR035C-A"
/db_xref="GeneID:1466430"
mRNA complement(<192337..>192417)
/locus_tag="YAR035C-A"
/product="uncharacterized protein"
/transcript_id="NM_001184583.1"
/db_xref="GeneID:1466430"
CDS complement(192337..192417)
/locus_tag="YAR035C-A"
/experiment="EXISTENCE:direct assay:GO:0005739
mitochondrion [PMID:32034123]"
/note="hypothetical protein; emerging ORF that arose de
novo from non-genic locus; identified by gene-trapping,
microarray-based expression analysis, and genome-wide
homology searching; localizes to mitochondria"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_878042.1"
/db_xref="GeneID:1466430"
/db_xref="SGD:S000028595"
/translation="MRLNYSRCYYSSQRRRQSLPKRFPLI"
gene <192619..>196185
/gene="SWH1"
/locus_tag="YAR042W"
/gene_synonym="OSH1; YAR044W"
/db_xref="GeneID:851286"
mRNA <192619..>196185
/gene="SWH1"
/locus_tag="YAR042W"
/gene_synonym="OSH1; YAR044W"
/product="oxysterol-binding protein related protein SWH1"
/transcript_id="NM_001178227.1"
/db_xref="GeneID:851286"
CDS 192619..196185
/gene="SWH1"
/locus_tag="YAR042W"
/gene_synonym="OSH1; YAR044W"
/experiment="EXISTENCE:direct assay:GO:0000138 Golgi trans
cisterna [PMID:11408574]"
/experiment="EXISTENCE:direct assay:GO:0005635 nuclear
envelope [PMID:12727870]"
/experiment="EXISTENCE:direct assay:GO:0005769 early
endosome [PMID:11408574]"
/experiment="EXISTENCE:direct assay:GO:0005783 endoplasmic
reticulum [PMID:12727870]"
/experiment="EXISTENCE:direct assay:GO:0008289 lipid
binding [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0015918 sterol
transport [PMID:20008566]"
/experiment="EXISTENCE:direct assay:GO:0071561
nucleus-vacuole junction [PMID:25420878]"
/experiment="EXISTENCE:direct assay:GO:0120015 sterol
transfer activity [PMID:20008566]"
/experiment="EXISTENCE:genetic interaction:GO:0006887
exocytosis [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0006897
endocytosis [PMID:15173322]"
/experiment="EXISTENCE:genetic interaction:GO:0015918
sterol transport [PMID:16585271]"
/experiment="EXISTENCE:genetic interaction:GO:0030011
maintenance of cell polarity [PMID:17004323]"
/experiment="EXISTENCE:genetic interaction:GO:0034727
piecemeal microautophagy of the nucleus [PMID:15367582]"
/note="Protein similar to mammalian oxysterol-binding
protein; contains ankyrin repeats and FFAT motif;
interacts with ER anchor Scs2p at the nucleus-vacuole
junction; regulated by sterol binding; SWH1 has a paralog,
OSH2, that arose from the whole genome duplication"
/codon_start=1
/product="oxysterol-binding protein related protein SWH1"
/protein_id="NP_009421.3"
/db_xref="GeneID:851286"
/db_xref="SGD:S000000081"
/translation="MEQPDLSSVAISKPLLKLKLLDALRQGSFPNLQDLLKKQFQPLD
DPNVQQVLHLMLHYAVQVAPMAVIKEIVHHWVSTTNTTFLNIHLDLNERDSNGNTPLH
IAAYQSRGDIVAFLLDQPTINDCVLNNSHLQAIEMCKNLNIAQMMQVKRSTYVAETAQ
EFRTAFNNRDFGHLESILSSPRNAELLDINGMDPETGDTVLHEFVKKRDVIMCRWLLE
HGADPFKRDRKGKLPIELVRKVNENDTATNTKIAIDIELKKLLERATREQSVIDVTNN
NLHEAPTYKGYLKKWTNFAQGYKLRWFILSSDGKLSYYIDQADTKNACRGSLNMSSCS
LHLDSSEKLKFEIIGGNNGVIRWHLKGNHPIETNRWVWAIQGAIRYAKDREILLHNGP
YSPSLALSHGLSSKVSNKENLHATSKRLTKSPHLSKSTLTQNDHDNDDDSTNNNNNKS
NNDYDDNNNNNNNDDDDYDDDDESRPLIEPLPLISSRSQSLSEITPGPHSRKSTVSST
RAADIPSDDEGYSEDDSDDDGNSSYTMENGGENDGDEDLNAIYGPYIQKLHMLQRSIS
IELASLNELLQDKQQHDEYWNTVNTSIETVSEFFDKLNRLTSQREKRMIAQMTKQRDV
NNVWIQSVKDLEMELVDKDEKLVALDKERKNLKKMLQKKLNNQPQVETEANEESDDAN
SMIKGSQESTNTLEEIVKFIEATKESDEDSDADEFFDAEEAASDKKANDSEDLTTNKE
TPANAKPQEEAPEDESLIVISSPQVEKKNQLLKEGSFVGYEDPVRTKLALDEDNRPKI
GLWSVLKSMVGQDLTKLTLPVSFNEPTSLLQRVSEDIEYSHILDQAATFEDSSLRMLY
VAAFTASMYASTTNRVSKPFNPLLGETFEYARTDGQYRFFTEQVSHHPPISATWTESP
KWDFYGECNVDSSFNGRTFAVQHLGLWYITIRPDHNISVPEETYSWKKPNNTVIGILM
GKPQVDNSGDVKVTNHTTGDYCMLHYKAHGWTSAGAYEVRGEVFNKDDKKLWVLGGHW
NDSIYGKKVTARGGELTLDRIKTANSATGGPKLDGSKFLIWKANERPSVPFNLTSFAL
TLNALPPHLIPYLAPTDSRLRPDQRAMENGEYDKAAAEKHRVEVKQRAAKKEREQKGE
EYRPKWFVQEEHPVTKSLYWKFNGEYWNKRKNHDFKDCADIF"
gene <203403..>208016
/gene="FLO1"
/locus_tag="YAR050W"
/gene_synonym="FLO2; FLO4"
/db_xref="GeneID:851289"
mRNA <203403..>208016
/gene="FLO1"
/locus_tag="YAR050W"
/gene_synonym="FLO2; FLO4"
/product="flocculin FLO1"
/transcript_id="NM_001178230.1"
/db_xref="GeneID:851289"
CDS 203403..208016
/gene="FLO1"
/locus_tag="YAR050W"
/gene_synonym="FLO2; FLO4"
/experiment="EXISTENCE:direct assay:GO:0009277 fungal-type
cell wall [PMID:9483793|PMID:11956747]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/experiment="EXISTENCE:mutant phenotype:GO:0000128
flocculation
[PMID:7483845|PMID:23383507|PMID:11027318|PMID:19013280|PM
ID:19420680]"
/experiment="EXISTENCE:mutant phenotype:GO:0005537
D-mannose binding [PMID:9851992]"
/note="Lectin-like protein involved in flocculation; cell
wall protein that binds mannose chains on the surface of
other cells, confers floc-forming ability that is
chymotrypsin sensitive and heat resistant; important for
co-flocculation with other yeasts, mediating interaction
with specific species; FLO1 has a paralog, FLO5, that
arose from a segmental duplication"
/codon_start=1
/product="flocculin FLO1"
/protein_id="NP_009424.1"
/db_xref="GeneID:851289"
/db_xref="SGD:S000000084"
/translation="MTMPHRYMFLAVFTLLALTSVASGATEACLPAGQRKSGMNINFY
QYSLKDSSTYSNAAYMAYGYASKTKLGSVGGQTDISIDYNIPCVSSSGTFPCPQEDSY
GNWGCKGMGACSNSQGIAYWSTDLFGFYTTPTNVTLEMTGYFLPPQTGSYTFKFATVD
DSAILSVGGATAFNCCAQQQPPITSTNFTIDGIKPWGGSLPPNIEGTVYMYAGYYYPM
KVVYSNAVSWGTLPISVTLPDGTTVSDDFEGYVYSFDDDLSQSNCTVPDPSNYAVSTT
TTTTEPWTGTFTSTSTEMTTVTGTNGVPTDETVIVIRTPTTASTIITTTEPWNSTFTS
TSTELTTVTGTNGVRTDETIIVIRTPTTATTAITTTEPWNSTFTSTSTELTTVTGTNG
LPTDETIIVIRTPTTATTAMTTTQPWNDTFTSTSTELTTVTGTNGLPTDETIIVIRTP
TTATTAMTTTQPWNDTFTSTSTELTTVTGTNGLPTDETIIVIRTPTTATTAMTTTQPW
NDTFTSTSTEITTVTGTNGLPTDETIIVIRTPTTATTAMTTPQPWNDTFTSTSTEMTT
VTGTNGLPTDETIIVIRTPTTATTAITTTEPWNSTFTSTSTEMTTVTGTNGLPTDETI
IVIRTPTTATTAITTTQPWNDTFTSTSTEMTTVTGTNGLPTDETIIVIRTPTTATTAM
TTTQPWNDTFTSTSTEITTVTGTTGLPTDETIIVIRTPTTATTAMTTTQPWNDTFTST
STEMTTVTGTNGVPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEMTTVTGTNGQ
PTDETVIVIRTPTSEGLVTTTTEPWTGTFTSTSTEMTTITGTNGVPTDETVIVIRTPT
SEGLISTTTEPWTGTFTSTSTEMTTITGTNGQPTDETVIVIRTPTSEGLISTTTEPWT
GTFTSTSTEMTHVTGTNGVPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEVTTI
TGTNGQPTDETVIVIRTPTSEGLISTTTEPWTGTFTSTSTEMTTVTGTNGQPTDETVI
VIRTPTSEGLVTTTTEPWTGTFTSTSTEMSTVTGTNGLPTDETVIVVKTPTTAISSSL
SSSSSGQITSSITSSRPIITPFYPSNGTSVISSSVISSSVTSSLFTSSPVISSSVISS
STTTSTSIFSESSKSSVIPTSSSTSGSSESETSSAGSVSSSSFISSESSKSPTYSSSS
LPLVTSATTSQETASSLPPATTTKTSEQTTLVTVTSCESHVCTESISPAIVSTATVTV
SGVTTEYTTWCPISTTETTKQTKGTTEQTTETTKQTTVVTISSCESDVCSKTASPAIV
STSTATINGVTTEYTTWCPISTTESRQQTTLVTVTSCESGVCSETASPAIVSTATATV
NDVVTVYPTWRPQTANEESVSSKMNSATGETTTNTLAAETTTNTVAAETITNTGAAET
KTVVTSSLSRSNHAETQTASATDVIGHSSSVVSVSETGNTKSLTSSGLSTMSQQPRST
PASSMVGYSTASLEISTYAGSANSLLAGSGLSVFIASLLLAII"
repeat_region complement(209448..209778)
/note="Ty1 LTR"
/rpt_type=long_terminal_repeat
/db_xref="SGD:S000006791"
rep_origin 214888..215644
/note="ARS111; Autonomously Replicating Sequence"
/db_xref="SGD:S000121256"
gene 218140..219145
/locus_tag="YAR061W"
/gene_synonym="YAR062W"
/note="Pseudogenic fragment with similarity to flocculins;
YAR061W has a paralog, YHR212W-A, that arose from a
segmental duplication"
/pseudo
/db_xref="GeneID:851292"
/db_xref="SGD:S000000087"
mRNA 218140..219145
/locus_tag="YAR061W"
/gene_synonym="YAR062W"
/pseudo
/db_xref="GeneID:851292"
/db_xref="SGD:S000000087"
gene <220198..>220497
/locus_tag="YAR064W"
/db_xref="GeneID:851294"
mRNA <220198..>220497
/locus_tag="YAR064W"
/product="uncharacterized protein"
/transcript_id="NM_001178235.1"
/db_xref="GeneID:851294"
CDS 220198..220497
/locus_tag="YAR064W"
/note="Pseudogenic fragment with similarity to flocculins;
YAR064W has a paralog, YHR213W-B, that arose from a
segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_009429.1"
/db_xref="GeneID:851294"
/db_xref="SGD:S000000089"
/translation="MLIDFCCSYIAGTHGRERAPSFTGTFVSHVSGENNCRPRRSEIT
QPCASGTEKKHFAATEKPCTNSLEGSRKDFLSLPLGHSYLFLFCFWRMICSEPKL"
gene <221049..>221660
/locus_tag="YAR066W"
/db_xref="GeneID:851295"
mRNA <221049..>221660
/locus_tag="YAR066W"
/product="uncharacterized protein"
/transcript_id="NM_001180045.1"
/db_xref="GeneID:851295"
CDS 221049..221660
/locus_tag="YAR066W"
/note="Putative GPI protein; SWAT-GFP and mCherry fusion
proteins localize to the endoplasmic reticulum and vacuole
respectively; YAR066W has a paralog, YHR214W, that arose
from a segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_009430.1"
/db_xref="GeneID:851295"
/db_xref="SGD:S000002144"
/translation="MFNRFNKFQAAVALALLSRGALGDSYTNSTSSADLSSITSVSSA
SASATASDSLSSSDGTVYLPSTTISGDLTVTGKVIATEAVEVAAGGKLTLLDGEKYVF
SSDLKVHGDLVVEKSEASYEGTAFDVSGETFEVSGNFSAEETGAVSASIYSFTPSSFK
SSGDISLSLSKAKKGEVTFSPYSNAGTFSLSNAILNGGSVSGL"
gene <222406..>222891
/locus_tag="YAR068W"
/db_xref="GeneID:851296"
mRNA <222406..>222891
/locus_tag="YAR068W"
/product="uncharacterized protein"
/transcript_id="NM_001178236.1"
/db_xref="GeneID:851296"
CDS 222406..222891
/locus_tag="YAR068W"
/note="Fungal-specific hypothetical protein; induced in
respiratory-deficient cells; YAR068W has a paralog,
YHR214W-A, that arose from a segmental duplication"
/codon_start=1
/product="uncharacterized protein"
/protein_id="NP_009431.1"
/db_xref="GeneID:851296"
/db_xref="SGD:S000000091"
/translation="MPQVQSWFPVQKQPTLAVTFTPLPQLSHAHLPLPPSHLVTKTDA
MFQHQLLPTQLQPFPPSHTPLLLLLTVTTMAVTPRLSLLNVLKKLQQPPFLQNHTLLL
PLLTVTTTAVTPRLSLPRLPNKHHWPLAQSPSLLLQLLILLLPAPSLVLSFNPKVWLL
V"
rep_origin 222880..224046
/note="ARS112; Autonomously Replicating Sequence"
/db_xref="SGD:S000121257"
gene <225460..>226863
/gene="PHO11"
/locus_tag="YAR071W"
/db_xref="GeneID:851299"
mRNA <225460..>226863
/gene="PHO11"
/locus_tag="YAR071W"
/product="acid phosphatase PHO11"
/transcript_id="NM_001178239.1"
/db_xref="GeneID:851299"
CDS 225460..226863
/gene="PHO11"
/locus_tag="YAR071W"
/EC_number="3.1.3.2"
/experiment="EXISTENCE:direct assay:GO:0003993 acid
phosphatase activity [PMID:8817921]"
/experiment="EXISTENCE:direct assay:GO:0005576
extracellular region [PMID:8817921]"
/experiment="EXISTENCE:direct assay:GO:0071944 cell
periphery [PMID:26928762]"
/note="One of three repressible acid phosphatases;
glycoprotein that is transported to the cell surface by
the secretory pathway; induced by phosphate starvation and
coordinately regulated by PHO4 and PHO2; PHO11 has a
paralog, PHO12, that arose from a segmental duplication"
/codon_start=1
/product="acid phosphatase PHO11"
/protein_id="NP_009434.1"
/db_xref="GeneID:851299"
/db_xref="SGD:S000000094"
/translation="MLKSAVYSILAASLVNAGTIPLGKLSDIDKIGTQTEIFPFLGGS
GPYYSFPGDYGISRDLPESCEMKQVQMVGRHGERYPTVSKAKSIMTTWYKLSNYTGQF
SGALSFLNDDYEFFIRDTKNLEMETTLANSVNVLNPYTGEMNAKRHARDFLAQYGYMV
ENQTSFAVFTSNSNRCHDTAQYFIDGLGDKFNISLQTISEAESAGANTLSAHHSCPAW
DDDVNDDILKKYDTKYLSGIAKRLNKENKGLNLTSSDANTFFAWCAYEINARGYSDIC
NIFTKDELVRFSYGQDLETYYQTGPGYDVVRSVGANLFNASVKLLKESEVQDQKVWLS
FTHDTDILNYLTTIGIIDDKNNLTAEHVPFMENTFHRSWYVPQGARVYTEKFQCSNDT
YVRYVINDAVVPIETCSTGPGFSCEINDFYDYAEKRVAGTDFLKVCNVSSVSNSTELT
FFWDWNTKHYNDTLLKQ"
telomere 229411..230218
/note="TEL01R; Telomeric region on the right arm of
Chromosome I; composed of an X element core sequence and a
short terminal stretch of telomeric repeats"
/db_xref="SGD:S000028937"
CONTIG join(BK006935.2:1..230218)
//
