LOCUS NC_076231 4512 bp RNA linear VRL 05-MAY-2023
DEFINITION Elderberry aureusvirus 1 isolate B15, complete genome.
ACCESSION NC_076231
VERSION NC_076231.1
DBLINK BioProject: PRJNA485481
KEYWORDS RefSeq.
SOURCE Elderberry aureusvirus 1
ORGANISM Elderberry aureusvirus 1
Viruses; Riboviria; Orthornavirae; Kitrinoviricota;
Tolucaviricetes; Tolivirales; Tombusviridae; Procedovirinae;
Aureusvirus; Aureusvirus sambuci.
REFERENCE 1 (bases 1 to 4512)
AUTHORS Safarova,D., Vavrouskova,K., Candresse,T. and Navratil,M.
TITLE Molecular characterization of a novel Aureusvirus infecting
elderberry (Sambucus nigra L.)
JOURNAL PLoS ONE 13 (8), e0200506 (2018)
PUBMED 30114234
REMARK Publication Status: Online-Only
REFERENCE 2 (bases 1 to 4512)
CONSRTM NCBI Genome Project
TITLE Direct Submission
JOURNAL Submitted (03-MAY-2023) National Center for Biotechnology
Information, NIH, Bethesda, MD 20894, USA
REFERENCE 3 (bases 1 to 4512)
AUTHORS Safarova,D., Vavrouskova,K., Candresse,T. and Navratil,M.
TITLE Direct Submission
JOURNAL Submitted (17-FEB-2018) Dept. of Cell Biology and Genetics, Fac. of
Science, Palacky University, Slechtitelu 27, Olomouc 783 71, Czech
Republic
COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final
NCBI review. The reference sequence is identical to MG967280.
##Assembly-Data-START##
Assembly Method :: CLC Genomic Workbench v. 9; SeqMan Pro,
Lasergene v. 12.3.1.4
Coverage :: 68x
Sequencing Technology :: Sanger dideoxy sequencing; Illumina
##Assembly-Data-END##
COMPLETENESS: full length.
FEATURES Location/Qualifiers
source 1..4512
/organism="Elderberry aureusvirus 1"
/mol_type="genomic RNA"
/isolate="B15"
/isolation_source="leaves"
/host="Sambucus nigra"
/db_xref="taxon:2304214"
/geo_loc_name="Czech Republic: Central Moravia"
/collection_date="13-Sep-2015"
5'UTR 1..102
gene 103..2382
/locus_tag="QKM04_gp1"
/db_xref="GeneID:80535610"
CDS 103..2382
/locus_tag="QKM04_gp1"
/note="ORF1-RT; readthrough stop codon; 85.3 kDa protein"
/codon_start=1
/transl_except=(pos:829..831,aa:OTHER)
/product="RNA-dependent RNA polymerase"
/protein_id="YP_010797628.1"
/db_xref="GeneID:80535610"
/translation="MAFSSLSYFLFLSLFHWCLWAVGLPVRACTGAYRSAVWLVVAVA
TLIGRVARVALFEAACAWGDIKAGVRVGLGSLSLSWLVVFALICFLSTCFVGLMPTLT
FLSICALCIGGKYGGRVPRYVRAHVERIQMAWAAGMEDDDCCEAAPTTGMDRITTRAR
PSVACKIAVRAISKVGILKRTEANAMVYQRLCLDVMGEMKMRYHDRLVILPQAVLACL
ERPAEVEEVMVAVKASCEGRLDNRXGCLLQHRGFNTAVKSAIPDGVLMNHEGMVVRKG
SPVKNERSWYSFAGYASTYEYIVHNSSLVNVCRGLVERVFCVVRDGKLQRPLKPKTNH
FERKLGDVGRRVSQIVGFCPAMTRTEFCASYSGKRRATYEEARLSLDVLPCTRKDAYL
KTFVKAEKINITLKPDPAPRVIQPRDPRYNVEVGRYLKPLEPRLMKAIDKLWGEKTAI
KGYTVEQVGTILHDKSLRFTDPVFVGLDASRFDQHCSRDALEWEHSVYNAIIRDPYLA
ELLKWQIDNRGTAYLKDGFVRYKVDGCRMSGDMNTSMGNYLIMSCLVYAFCKDVGLDA
SLANCGDDCVLYLERKNLNLLKALPQWFENMGYSMKVERPVHLVEEIEFCQQHPVQLS
RGWVMVRRPDVCLTKDVCVVRGGMTPERLQRWLYAQRDGGLSLAGDCPVLAAFYAAFP
KGDAQGEESEYAEPHKFKASEQCSEITSLSRYSFWLAFGLTPDEQIAIERELGEWKPV
VRMGDACTRPTLLDYCSRY"
misc_feature 463..804
/locus_tag="QKM04_gp1"
/note="Tombusvirus p33; Region: Tombus_P33; pfam08500"
/db_xref="CDD:149522"
misc_feature 907..2370
/locus_tag="QKM04_gp1"
/note="RNA-dependent RNA polymerase (RdRp) in the genus
Aureusvirus of positive-sense single-stranded RNA
[(+)ssRNA] viruses, within the Procedovirinae subfamily;
Region: Aureusvirus_RdRp; cd23243"
/db_xref="CDD:438093"
misc_feature 1519..1563
/locus_tag="QKM04_gp1"
/note="conserved polymerase motif A; other site"
/db_xref="CDD:438093"
misc_feature 1708..1779
/locus_tag="QKM04_gp1"
/note="conserved polymerase motif B; other site"
/db_xref="CDD:438093"
misc_feature 1798..1842
/locus_tag="QKM04_gp1"
/note="conserved polymerase motif C; other site"
/db_xref="CDD:438093"
gene 103..831
/locus_tag="QKM04_gp2"
/db_xref="GeneID:80535607"
CDS 103..831
/locus_tag="QKM04_gp2"
/note="26.5 kDa protein; ORF1"
/codon_start=1
/product="replicase associated protein"
/protein_id="YP_010797629.1"
/db_xref="GeneID:80535607"
/translation="MAFSSLSYFLFLSLFHWCLWAVGLPVRACTGAYRSAVWLVVAVA
TLIGRVARVALFEAACAWGDIKAGVRVGLGSLSLSWLVVFALICFLSTCFVGLMPTLT
FLSICALCIGGKYGGRVPRYVRAHVERIQMAWAAGMEDDDCCEAAPTTGMDRITTRAR
PSVACKIAVRAISKVGILKRTEANAMVYQRLCLDVMGEMKMRYHDRLVILPQAVLACL
ERPAEVEEVMVAVKASCEGRLDNR"
misc_feature 463..804
/locus_tag="QKM04_gp2"
/note="Tombusvirus p33; Region: Tombus_P33; pfam08500"
/db_xref="CDD:149522"
gene 2410..3543
/locus_tag="QKM04_gp3"
/db_xref="GeneID:80535606"
CDS 2410..3543
/locus_tag="QKM04_gp3"
/note="39.6 kDa protein, CP; ORF2"
/codon_start=1
/product="coat protein"
/protein_id="YP_010797630.1"
/db_xref="GeneID:80535606"
/translation="MALVLRNPNLAITPAMKAAGGQVVMQIGTMMIKKGKQMFWNGVR
WVTRQAYNAMVNGGTRRGITQAGALSGAVVAPVAVARIVRGSKPKFRKGVGSVTISHR
ELLGQWNNSPGLVVNQGVADNIYRVNPSNGVLFPWLQTLASNFDQYTFGNVSLQYVPL
CSTTETGRVAMYFDKDSQDLEPADRVELASMAHLTETSAWAEATLNIPTDGLKRFTDD
SATADPKLLDLGQIGVATYGGSGTNPVGDIFIRYTITFHEPQASSGLVSTIQTGTGSI
SSGPTIVSVSGSDTSTTVTFRGPGTFYVNMGQRATALANVGNSAGTTVSSITNASNGT
IYTSTCVIIVNTAGGTITYTGTAFGNYTLNVTRTKRVTRTNII"
misc_feature 2650..3192
/locus_tag="QKM04_gp3"
/note="Viral coat protein (S domain); Region: Viral_coat;
pfam00729"
/db_xref="CDD:395591"
misc_feature 3127..>3528
/locus_tag="QKM04_gp3"
/note="BDLF3; Provisional; Region: PHA03255"
/db_xref="CDD:165513"
gene 3624..4334
/locus_tag="QKM04_gp4"
/db_xref="GeneID:80535608"
CDS 3624..4334
/locus_tag="QKM04_gp4"
/note="26 kDa protein, MP; ORF3"
/codon_start=1
/product="movement protein"
/protein_id="YP_010797631.1"
/db_xref="GeneID:80535608"
/translation="MEIQSLDGKFEESLIVQNEVKKVSLSHKTTKAILPLAPLSQFAK
WKIPKTGFYAPIDVKFVLTPHISERAQVRGVVKLVDSRDLAPSRELFKSREFNIGHGL
VIEGSQLPFCLPVGDYPLQFEVTVLQSQFRETSTLYTTSVEWRMMSSTTPLSRVKSVM
GTAQQQAMSITPNFKMSLESSKGATAVSRKKKTNKPNGLGVRGHGDSSVGGSSDDLPH
SVHAWEGHHYPLGDSLSQ"
misc_feature 3639..4172
/locus_tag="QKM04_gp4"
/note="TBSV core protein P21/P22; Region: TBSV_P22;
pfam03558"
/db_xref="CDD:367561"
gene 3755..4141
/locus_tag="QKM04_gp5"
/db_xref="GeneID:80535609"
CDS 3755..4141
/locus_tag="QKM04_gp5"
/note="14 kDa; ORF4"
/codon_start=1
/product="RNA silencing suppressor"
/protein_id="YP_010797632.1"
/db_xref="GeneID:80535609"
/translation="MEDPKDGILCTNRCQICSHTTYIRESSGQGGRKTCRLKGFSTQP
RIVQEQGIQYRSWIGDRGFPATLLSSSGGLSTTIRGHGVTITIQGDVDAVHNISRVAY
DVFHHPVIQSEVCYGNSTTTSDEHHA"
ORIGIN
1 tcttgagagt tgtcgagcca tcgatatagg ttttctctct tatttcataa gggtttgaag
61 gtgaggtcta gcttttgcgg ataaattgta acttctcaca aaatggcgtt ctcttctctg
121 tcttactttc tgtttcttag tctttttcac tggtgtctat gggctgtcgg attacctgtc
181 cgggcctgta ctggagccta caggtctgct gtctggctcg tggtggctgt tgccacgctg
241 atcggacggg tggccagggt ggccctgttt gaggcggcgt gtgcatgggg ggatatcaag
301 gcgggggtgc gggttggctt ggggagttta tcgctctcct ggctagttgt ctttgcactc
361 atttgctttc tctcaacgtg ttttgtcggg cttatgccca ccctcacttt cctgtctatc
421 tgtgctttat gcatcggcgg gaagtatggg ggtcgtgtgc cacggtatgt acgggcacat
481 gttgagagga tccagatggc atgggctgcc ggaatggagg atgacgactg ctgtgaggct
541 gcccccacta ctgggatgga tcgcatcacc actcgggctc gccccagtgt tgcgtgcaag
601 atagctgtta gggctatatc aaaagtgggg atacttaagc ggaccgaggc taatgccatg
661 gtctaccagc ggttgtgctt ggatgtgatg ggggagatga agatgcgata tcacgatcga
721 ctcgtcatct tgccccaggc tgtgctcgct tgtctggagc ggccagcaga ggtggaggaa
781 gtgatggtgg ctgttaaggc gtcctgtgag gggcgtcttg acaaccgcta ggggtgccta
841 ttgcaacacc gtggttttaa cacggccgtc aagagcgcaa taccggacgg cgtgttaatg
901 aaccatgagg gtatggtggt tcgcaaagga tcccctgtga agaatgaacg gagttggtac
961 tcatttgctg gatatgccag cacgtacgag tacattgtgc ataattcctc gctcgtcaac
1021 gtttgtcgcg gtttagttga acgagtgttt tgcgtcgtgc gagacggtaa attgcagcgg
1081 cctctcaagc caaagactaa tcattttgag aggaagctag gtgatgttgg tcgtagagtt
1141 agccaaattg tggggttctg tcccgctatg acacgcacgg aattctgtgc gtcttacagc
1201 ggcaaacgta gagccaccta tgaggaggca cgactgtcct tggatgttct cccctgcacg
1261 cgaaaggatg cttacctgaa aacttttgtg aaagcggaga agatcaacat tacactgaaa
1321 cccgaccctg cacctagggt catacagcca cgtgatccta ggtacaatgt ggaggtggga
1381 cgatatctaa agcctcttga acccagacta atgaaagcaa ttgacaaact ctggggggag
1441 aagacggcta taaagggtta cactgttgag caagtaggta cgatactgca tgacaaatct
1501 ctcaggttca ctgaccccgt gttcgtgggg cttgatgcta gtcgctttga ccagcattgc
1561 tctagggatg cacttgagtg ggaacacagt gtgtacaacg cgattattcg tgacccgtat
1621 ttggcagagc tgctgaaatg gcaaattgac aaccgtggca cggcctatct taaggatggc
1681 tttgtccggt ataaggtcga tgggtgccgt atgtctggtg atatgaacac tagcatgggc
1741 aattacctca tcatgagttg cctggtctac gccttttgca aggacgttgg actcgacgcc
1801 agcttggcca actgcgggga tgactgcgtt ttgtaccttg agagaaagaa tttgaacctg
1861 cttaaagctc tacctcaatg gttcgagaac atgggctact ctatgaaagt agagcggcca
1921 gtacatttgg ttgaggagat agagttttgt caacagcacc cggtgcaact tagtagaggg
1981 tgggtgatgg ttaggaggcc tgacgtctgt ttgacgaagg atgtgtgtgt tgtccgagga
2041 gggatgaccc cggagcgatt gcagagatgg ctgtatgcac agcgggacgg aggccttagc
2101 ttggccggtg actgtcctgt cctagcagcc ttttatgcag ctttcccaaa aggggatgct
2161 cagggggaag aatccgaata cgctgaacca cacaaattta aggcaagtga gcaatgcagc
2221 gagatcactt cgctttcccg atactctttt tggctggctt tcgggttgac acccgatgag
2281 cagatcgcca ttgagaggga gttgggggag tggaaaccag ttgtgaggat gggtgacgca
2341 tgtacaagac ccaccttgct ggactattgc tcaagatact aaccacatca cacgcaaacg
2401 attattatta tggcgctcgt attacgaaat ccgaatttgg ctattacacc agcaatgaag
2461 gcagctgggg ggcaggtggt catgcagatt ggaactatga tgattaagaa agggaaacag
2521 atgttttgga acggggtgcg ctgggtgaca cgtcaagctt acaatgctat ggtcaacggc
2581 ggcacacgga gaggtattac gcaggctggt gccctgtcgg gggcggttgt tgctcccgtc
2641 gcggtcgcta gaatcgtgcg gggaagtaaa cccaagttta ggaaaggagt tggatctgtc
2701 accatttctc atcgggaact gttagggcag tggaacaatt caccaggatt ggttgtgaat
2761 cagggagtcg cagacaacat ctaccgggta aaccctagta atggcgtcct cttcccttgg
2821 ttacagacgt tagcatcaaa ctttgaccag tatacttttg gcaatgtgtc gctgcagtat
2881 gtccctcttt gttctacaac ggagactggg cgagtggcga tgtatttcga caaggactca
2941 caagacctag aaccggcaga tagggtggag ctagcaagta tggcccacct tacagagacc
3001 agtgcatggg cagaggccac gttgaacatt ccgactgacg ggttgaagag atttactgac
3061 gatagtgcca cagctgatcc gaaattacta gacctggggc aaattggtgt ggccacttat
3121 ggcggatctg ggaccaaccc tgttggcgac attttcatac ggtacaccat cactttccat
3181 gaaccgcaag cctcctctgg actcgtttct actatacaga cggggactgg tagcatcagt
3241 tcggggccga ccattgtgtc tgtatccggc agtgacacca gcaccacggt cacctttaga
3301 gggcccggca cgttttacgt caatatgggg cagagggcaa ccgcccttgc taatgttggc
3361 aattcggctg ggaccactgt atcatcgatt actaacgcca gcaatggaac gatttacaca
3421 tcaacctgtg ttataattgt aaacactgcg ggcgggacca ttacctacac cggcactgct
3481 ttcgggaatt acacgctgaa tgtaacacgg accaagaggg tcacacgaac gaacataata
3541 tgatggtacc aacggtaggg tgaatgccat gcttgacaat gtctcgctta ggcttgggtg
3601 gctcaagaag tttaaccagc tttatggaaa tacagtctct tgacggaaaa tttgaggaat
3661 ctttgattgt tcagaacgag gttaagaaag tatcactatc tcataaaact acaaaagcca
3721 tactaccgct agcaccactc agtcagtttg caaaatggaa gatcccaaag acgggatttt
3781 atgcaccaat agatgtcaaa tttgttctca caccacatat atcagagaga gctcaggtca
3841 ggggggtcgt aaaacttgtc gactcaaggg atttagcacc cagccgcgaa ttgttcaaga
3901 gcagggaatt caatatcggt catggattgg tgatcgaggg ttcccagcta cccttctgtc
3961 ttccagtggg ggactatcca ctacaattcg aggtcacggt gttacaatca caattcaggg
4021 agacgtcgac gctgtacaca acatcagtcg agtggcgtat gatgtcttcc accaccccgt
4081 tatccagagt gaagtctgtt atgggaacag cacaacaaca agcgatgagc atcacgccta
4141 atttcaaaat gtcgcttgaa tcatccaagg gcgccactgc agtgtccagg aagaagaaaa
4201 caaacaagcc aaatggccta ggggtcagag gtcatggtga ttcgtctgtt ggtggttctt
4261 cagacgatct gccccacagc gtacatgcgt gggagggtca ccattatccc ctaggagaca
4321 gcttatctca gtagtcttct tgtcacatct gtaagtgagc acggtcaggc accggattct
4381 aaagattgca ataacccgca cttattaacc tgaaagtatg gtcaaaaata tgcggagcca
4441 accaatctga agtaattccg ggggctataa gcctgactgt tattcccgta taatccctcg
4501 cgcttcggcg ct
//
