ID A0A087EQV4_9LACO Unreviewed; 370 AA.
AC A0A087EQV4;
DT 29-OCT-2014, integrated into UniProtKB/TrEMBL.
DT 29-OCT-2014, sequence version 1.
DT 28-JAN-2026, entry version 59.
DE RecName: Full=RNA polymerase sigma factor SigA {ECO:0000256|HAMAP-Rule:MF_00963};
GN Name=rpoD {ECO:0000313|EMBL:KOY76289.1};
GN Synonyms=sigA {ECO:0000256|HAMAP-Rule:MF_00963};
GN ORFNames=APS55_06100 {ECO:0000313|EMBL:ALJ31801.1}, FF306_00056
GN {ECO:0000313|EMBL:GAT89965.1}, RZ71_07390
GN {ECO:0000313|EMBL:KOY76289.1}, RZ72_12680
GN {ECO:0000313|EMBL:KOY79110.1}, RZ78_12570
GN {ECO:0000313|EMBL:KPN82145.1};
OS Apilactobacillus kunkeei.
OC Bacteria; Bacillati; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Apilactobacillus.
OX NCBI_TaxID=148814 {ECO:0000313|EMBL:KOY76289.1, ECO:0000313|Proteomes:UP000037778};
RN [1] {ECO:0000313|Proteomes:UP000037749, ECO:0000313|Proteomes:UP000037778}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LAko {ECO:0000313|EMBL:KOY76289.1,
RC ECO:0000313|Proteomes:UP000037778}, LAla {ECO:0000313|EMBL:KOY79110.1,
RC ECO:0000313|Proteomes:UP000037749}, and LMbo
RC {ECO:0000313|EMBL:KPN82145.1, ECO:0000313|Proteomes:UP000050269};
RX PubMed=25953738; DOI=10.1093/gbe/evv079;
RA Tamarit D., Ellegaard K.M., Wikander J., Olofsson T., Vasquez A.,
RA Andersson S.G.;
RT "Functionally Structured Genomes in Lactobacillus kunkeei Colonizing the
RT Honey Crop and Food Products of Honeybees and Stingless Bees.";
RL Genome Biol. Evol. 7:1455-1473(2015).
RN [2] {ECO:0000313|Proteomes:UP000067203}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MP2 {ECO:0000313|Proteomes:UP000067203};
RA Asenjo F., Olmos A., Henriquez-Piskulich P., Aldea P., Ugalde J.A.,
RA Trombert A.N.;
RT "Bioinformatic analysis of the first complete genome sequence of
RT Lactobacillus kunkeei strain MP2, an Apis mellifera gut isolate.";
RL Submitted (OCT-2015) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:ALJ31801.1, ECO:0000313|Proteomes:UP000067203}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MP2 {ECO:0000313|EMBL:ALJ31801.1,
RC ECO:0000313|Proteomes:UP000067203};
RX PubMed=27114887;
RA Asenjo F., Olmos A., Henriquez-Piskulich P., Polanco V., Aldea P.,
RA Ugalde J.A., Trombert A.N.;
RT "Genome sequencing and analysis of the first complete genome of
RT Lactobacillus kunkeei strain MP2, an Apis mellifera gut isolate.";
RL PeerJ 4:E1950-E1950(2016).
RN [4] {ECO:0000313|EMBL:GAT89965.1, ECO:0000313|Proteomes:UP000186588}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FF30-6 {ECO:0000313|EMBL:GAT89965.1,
RC ECO:0000313|Proteomes:UP000186588};
RX PubMed=27776911; DOI=10.1016/j.syapm.2016.09.006;
RA Maeno S., Tanizawa Y., Kanesaki Y., Kubota E., Kumar H., Dicks L.,
RA Salminen S., Nakagawa J., Arita M., Endo A.;
RT "Genomic characterization of a fructophilic bee symbiont Lactobacillus
RT kunkeei reveals its niche-specific adaptation.";
RL Syst. Appl. Microbiol. 39:516-526(2016).
CC -!- FUNCTION: Sigma factors are initiation factors that promote the
CC attachment of RNA polymerase to specific initiation sites and are then
CC released. This sigma factor is the primary sigma factor during
CC exponential growth. {ECO:0000256|HAMAP-Rule:MF_00963}.
CC -!- SUBUNIT: Interacts transiently with the RNA polymerase catalytic core.
CC {ECO:0000256|HAMAP-Rule:MF_00963}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00963}.
CC -!- SIMILARITY: Belongs to the sigma-70 factor family. RpoD/SigA subfamily.
CC {ECO:0000256|HAMAP-Rule:MF_00963}.
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DR EMBL; CP012920; ALJ31801.1; -; Genomic_DNA.
DR EMBL; BDDX01000001; GAT89965.1; -; Genomic_DNA.
DR EMBL; JXCY01000006; KOY76289.1; -; Genomic_DNA.
DR EMBL; JXCZ01000019; KOY79110.1; -; Genomic_DNA.
DR EMBL; JXDF01000017; KPN82145.1; -; Genomic_DNA.
DR RefSeq; WP_034530776.1; NZ_BAABVW010000113.1.
DR AlphaFoldDB; A0A087EQV4; -.
DR STRING; 148814.APS55_06100; -.
DR KEGG; lku:APS55_06100; -.
DR PATRIC; fig|148814.10.peg.806; -.
DR eggNOG; COG0568; Bacteria.
DR OrthoDB; 9809557at2; -.
DR Proteomes; UP000037749; Unassembled WGS sequence.
DR Proteomes; UP000037778; Unassembled WGS sequence.
DR Proteomes; UP000050269; Unassembled WGS sequence.
DR Proteomes; UP000067203; Chromosome.
DR Proteomes; UP000186588; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016987; F:sigma factor activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006352; P:DNA-templated transcription initiation; IEA:UniProtKB-UniRule.
DR CDD; cd06171; Sigma70_r4; 1.
DR FunFam; 1.10.10.10:FF:000002; RNA polymerase sigma factor SigA; 1.
DR FunFam; 1.10.10.10:FF:000004; RNA polymerase sigma factor SigA; 1.
DR FunFam; 1.10.601.10:FF:000001; RNA polymerase sigma factor SigA; 1.
DR Gene3D; 1.10.601.10; RNA Polymerase Primary Sigma Factor; 2.
DR Gene3D; 1.10.220.120; Sigma-70 factor, region 1.1; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 2.
DR HAMAP; MF_00963; Sigma70_RpoD_SigA; 1.
DR InterPro; IPR014284; RNA_pol_sigma-70_dom.
DR InterPro; IPR000943; RNA_pol_sigma70.
DR InterPro; IPR009042; RNA_pol_sigma70_r1_2.
DR InterPro; IPR007627; RNA_pol_sigma70_r2.
DR InterPro; IPR007624; RNA_pol_sigma70_r3.
DR InterPro; IPR007630; RNA_pol_sigma70_r4.
DR InterPro; IPR007127; RNA_pol_sigma_70_r1_1.
DR InterPro; IPR042189; RNA_pol_sigma_70_r1_1_sf.
DR InterPro; IPR013325; RNA_pol_sigma_r2.
DR InterPro; IPR013324; RNA_pol_sigma_r3/r4-like.
DR InterPro; IPR012760; RNA_pol_sigma_RpoD_C.
DR InterPro; IPR050239; Sigma-70_RNA_pol_init_factors.
DR InterPro; IPR028630; Sigma70_RpoD.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR NCBIfam; NF006666; PRK09210.1; 1.
DR NCBIfam; TIGR02393; RpoD_Cterm; 1.
DR NCBIfam; TIGR02937; sigma70-ECF; 1.
DR PANTHER; PTHR30603; RNA POLYMERASE SIGMA FACTOR RPO; 1.
DR PANTHER; PTHR30603:SF60; RNA POLYMERASE SIGMA FACTOR RPOD; 1.
DR Pfam; PF03979; Sigma70_r1_1; 1.
DR Pfam; PF00140; Sigma70_r1_2; 1.
DR Pfam; PF04542; Sigma70_r2; 1.
DR Pfam; PF04539; Sigma70_r3; 1.
DR Pfam; PF04545; Sigma70_r4; 1.
DR PRINTS; PR00046; SIGMA70FCT.
DR SUPFAM; SSF88946; Sigma2 domain of RNA polymerase sigma factors; 1.
DR SUPFAM; SSF88659; Sigma3 and sigma4 domains of RNA polymerase sigma factors; 2.
DR PROSITE; PS00715; SIGMA70_1; 1.
DR PROSITE; PS00716; SIGMA70_2; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_00963};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00963}; Reference proteome {ECO:0000313|Proteomes:UP000037778};
KW Sigma factor {ECO:0000256|ARBA:ARBA00023082, ECO:0000256|HAMAP-
KW Rule:MF_00963};
KW Transcription {ECO:0000256|ARBA:ARBA00023163, ECO:0000256|HAMAP-
KW Rule:MF_00963};
KW Transcription regulation {ECO:0000256|ARBA:ARBA00023015, ECO:0000256|HAMAP-
KW Rule:MF_00963}.
FT DOMAIN 161..174
FT /note="RNA polymerase sigma-70"
FT /evidence="ECO:0000259|PROSITE:PS00715"
FT DOMAIN 330..356
FT /note="RNA polymerase sigma-70"
FT /evidence="ECO:0000259|PROSITE:PS00716"
FT DNA_BIND 331..350
FT /note="H-T-H motif"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00963"
FT REGION 137..207
FT /note="Sigma-70 factor domain-2"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00963"
FT REGION 216..292
FT /note="Sigma-70 factor domain-3"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00963"
FT REGION 305..358
FT /note="Sigma-70 factor domain-4"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00963"
FT MOTIF 161..164
FT /note="Interaction with polymerase core subunit RpoC"
FT /evidence="ECO:0000256|HAMAP-Rule:MF_00963"
SQ SEQUENCE 370 AA; 42059 MW; 0A6FF71B72EF0FA1 CRC64;
MAEKKLNKKE LKESTNALIK KQKKVGHVTY DDVNDTIAVP FKLTKTEMYE LLEKIEDAGI
SVVDKNGDPD PRALEAAKKV TQAELKNSAS APTGVKINDP VRMYLKEIGR VPLLTADQEV
DLALRIEEGD EEAKQELAEA NLRLVVSIAK RYVGRGMQFL DLIQEGNMGL MKAVEKFDYR
KGFKFSTYAT WWIRQAITRA IADQARTIRI PVHMVETINK LIRIQRQLLQ DLGREPTPEE
IGAEMDMPTQ KVRSILKISQ EPVSLETPIG EEDDSHLGDF IEDQDATSPA DHAAYELLKE
QLEGVLDTLT DREENVLRLR FGLDDGRTRT LEEVGKVFGV TRERIRQIEA KALRKLRHPS
RSKQLKDFLE
//
