ID A0A096S2D9_MAIZE Unreviewed; 349 AA.
AC A0A096S2D9;
DT 26-NOV-2014, integrated into UniProtKB/TrEMBL.
DT 26-NOV-2014, sequence version 1.
DT 18-JUN-2025, entry version 70.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN Name=LOC100280727 {ECO:0000313|EnsemblPlants:Zm00001eb312930_P001};
GN ORFNames=ZEAMMB73_Zm00001d020501 {ECO:0000313|EMBL:ONM55017.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577 {ECO:0000313|EMBL:ONM55017.1};
RN [1] {ECO:0000313|EMBL:ONM55017.1, ECO:0000313|Proteomes:UP000007305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73 {ECO:0000313|Proteomes:UP000007305};
RC TISSUE=Seedling {ECO:0000313|EMBL:ONM55017.1};
RG Maize Genome Sequencing Project;
RA Ware D.;
RT "Update maize B73 reference genome by single molecule sequencing
RT technologies.";
RL Submitted (DEC-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EnsemblPlants:Zm00001eb312930_P001}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73 {ECO:0000313|EnsemblPlants:Zm00001eb312930_P001};
RA Seetharam A., Woodhouse M., Cannon E.;
RL Submitted (JUL-2019) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EnsemblPlants:Zm00001eb312930_P001}
RP IDENTIFICATION.
RC STRAIN=cv. B73 {ECO:0000313|EnsemblPlants:Zm00001eb312930_P001};
RG EnsemblPlants;
RL Submitted (MAY-2021) to UniProtKB.
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
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DR EMBL; CM007650; ONM55017.1; -; Genomic_DNA.
DR RefSeq; NP_001147119.2; NM_001153647.2.
DR RefSeq; XP_008651386.1; XM_008653164.1.
DR RefSeq; XP_035816457.1; XM_035960564.1.
DR FunCoup; A0A096S2D9; 318.
DR IntAct; A0A096S2D9; 4.
DR STRING; 4577.A0A096S2D9; -.
DR PaxDb; 4577-GRMZM2G125867_P02; -.
DR EnsemblPlants; Zm00001eb312930_T001; Zm00001eb312930_P001; Zm00001eb312930.
DR EnsemblPlants; Zm00001eb312930_T002; Zm00001eb312930_P002; Zm00001eb312930.
DR GeneID; 100280727; -.
DR Gramene; Zm00001eb312930_T001; Zm00001eb312930_P001; Zm00001eb312930.
DR Gramene; Zm00001eb312930_T002; Zm00001eb312930_P002; Zm00001eb312930.
DR eggNOG; KOG1812; Eukaryota.
DR HOGENOM; CLU_022048_0_0_1; -.
DR OMA; ALMSQVM; -.
DR OrthoDB; 10009520at2759; -.
DR Proteomes; UP000007305; Chromosome 7.
DR ExpressionAtlas; A0A096S2D9; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0000151; C:ubiquitin ligase complex; IBA:GO_Central.
DR GO; GO:0031624; F:ubiquitin conjugating enzyme binding; IBA:GO_Central.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd22582; BRcat_RBR_unk; 1.
DR CDD; cd22584; Rcat_RBR_unk; 1.
DR FunFam; 1.20.120.1750:FF:000018; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000230; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 1.
DR SMART; SM00647; IBR; 2.
DR SMART; SM00184; RING; 3.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 2.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000007305};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 136..349
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 140..185
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
SQ SEQUENCE 349 AA; 38351 MW; 5A328E84029D5FD8 CRC64;
MLDRVAMSAE DSALAEDLQV EEVLRFSIQS GKACAVCKEG IRSLDASWKP ENCDHVICIA
CFCQYAPETE ATGLPRCAVA SCDSLCNTET HQGTNVPRCT SISIEDMDRK GKKPLGSSTI
QELGQCSRGA STNISSELYC AICMETVHIG ESFPVDGCTH TFCISCVSQC IAAKVEENVL
SIGCPAPGCK DGVLHPDACR DVIPAQLFQR WGAALCDSSL GSLKFYCPFK ECSALLVDDP
GHGEEVITNV ECPHCCRMFC AQCKVPWHAG VTCAEFQRLG KDERGREDLL LRKVAQESKW
QRCPKCKIYV ERIEGCVFII CRCGHCFCYL CASPMSRDNH CCKTCNRTW
//
