ID A0A0A3J0J9_9BACL Unreviewed; 726 AA.
AC A0A0A3J0J9;
DT 04-FEB-2015, integrated into UniProtKB/TrEMBL.
DT 04-FEB-2015, sequence version 1.
DT 28-JAN-2026, entry version 40.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN ORFNames=CD30_11070 {ECO:0000313|EMBL:KGR90476.1};
OS Ureibacillus massiliensis 4400831 = CIP 108448 = CCUG 49529.
OC Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Caryophanaceae;
OC Ureibacillus.
OX NCBI_TaxID=1211035 {ECO:0000313|EMBL:KGR90476.1, ECO:0000313|Proteomes:UP000030595};
RN [1] {ECO:0000313|EMBL:KGR90476.1, ECO:0000313|Proteomes:UP000030595}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CCUG 49529 {ECO:0000313|EMBL:KGR90476.1,
RC ECO:0000313|Proteomes:UP000030595};
RA Zhang F., Wang G., Zhang L.;
RT "Draft genome sequence of Lysinibacillus massiliensis CCUG 49529.";
RL Submitted (FEB-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KGR90476.1}.
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DR EMBL; JPVQ01000018; KGR90476.1; -; Genomic_DNA.
DR RefSeq; WP_036176604.1; NZ_AVCZ01000018.1.
DR AlphaFoldDB; A0A0A3J0J9; -.
DR eggNOG; COG0550; Bacteria.
DR OrthoDB; 9803554at2; -.
DR Proteomes; UP000030595; Unassembled WGS sequence.
DR GO; GO:0043597; C:cytoplasmic replication fork; IEA:TreeGrafter.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:TreeGrafter.
DR GO; GO:0006281; P:DNA repair; IEA:TreeGrafter.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR023406; Topo_IA_AS.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR005738; TopoIII.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_dom.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR NCBIfam; NF005829; PRK07726.1; 1.
DR NCBIfam; TIGR01056; topB; 1.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13342; Toprim_Crpt; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS00396; TOPO_IA_1; 1.
DR PROSITE; PS52039; TOPO_IA_2; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:KGR90476.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000030595};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 3..142
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT DOMAIN 159..603
FT /note="Topo IA-type catalytic"
FT /evidence="ECO:0000259|PROSITE:PS52039"
SQ SEQUENCE 726 AA; 82507 MW; F7BA23310B278938 CRC64;
MGRFLVIAEK PDQAAKLASP FSFTKKGGYF EVKPNSYFPE GALITWAIGH LCELKAPEEY
NPTWKKWTLE TLPIIPNRFE YKVSKSKYKQ FNVIKGLVKR PDIQEIIIGG DAGREGELIV
RTILKVCGVN KPMKRLWISS LTENAVKNGF SNLLPEEKTR DIYFEALSRS CADWLIGINA
SRLYTVLLKK NGVKDVFSIG RVQTPTLALI VKREHEIRDF KPETFWEVEA EFNFDGKILK
AKWHNNNNSK VQEERQAIAI ANFCINKNVV ITSIKKDTKE YVPPLLFNLS TLQATANKTY
KFSPQSTLDI LQKLYLKGLV SYPRSDSQFL TNEEAKTLPN ILESLGQIDR YKKLLPPPTI
SIMNNKRYVN EKKVTDHHAI IITEQLPNFS KLSSEEEKIY DLVVRQVIAA HYNNAIFSYT
TIHSLVDTRA EFISKGKVII EEGWRKVLYH SKDNTSEDED DLLPLLHENE QGLVSKSNVK
RSETQPPNRY SEGNLITLMK TAGKHLEDTE LEKVLSKTEG LGTEATRAGI IGTLKDRNYI
EVKKNQVFAT TKGMLLIEAL GESILTSPSM TAKWEQRLAE IGLGQASPKT FIEQVKKLAY
KLTTDANERE KEWAFNENVV EKISSNNPYN KKYKKEKTII GKCLLCGGSI LDHGTFYGCS
NYKSANCKFS VSKKVLSKTI SQANMKKLLT NGETDLIKGF KKGEKIFNAH LTWDIKAQKT
NFKFPQ
//
