ID A0A0E0CDE0_9ORYZ Unreviewed; 697 AA.
AC A0A0E0CDE0;
DT 27-MAY-2015, integrated into UniProtKB/TrEMBL.
DT 27-MAY-2015, sequence version 1.
DT 18-JUN-2025, entry version 35.
DE RecName: Full=beta-fructofuranosidase {ECO:0000256|ARBA:ARBA00012758};
DE EC=3.2.1.26 {ECO:0000256|ARBA:ARBA00012758};
OS Oryza meridionalis.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza.
OX NCBI_TaxID=40149 {ECO:0000313|EnsemblPlants:OMERI01G41910.1};
RN [1] {ECO:0000313|EnsemblPlants:OMERI01G41910.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (APR-2015) to UniProtKB.
RN [2] {ECO:0000313|EnsemblPlants:OMERI01G41910.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. OR44 {ECO:0000313|EnsemblPlants:OMERI01G41910.1};
RA Zhang J., Kudrna D., Lee S., Talag J., Welchert J., Wing R.A.;
RT "OmerRS3 (Oryza meridionalis Reference Sequence Version 3).";
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal non-reducing beta-D-fructofuranoside
CC residues in beta-D-fructofuranosides.; EC=3.2.1.26;
CC Evidence={ECO:0000256|ARBA:ARBA00000094};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}. Secreted, cell
CC wall {ECO:0000256|ARBA:ARBA00004191}.
CC -!- SIMILARITY: Belongs to the OB-RGRP/VPS55 family.
CC {ECO:0000256|ARBA:ARBA00005645}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 32 family.
CC {ECO:0000256|ARBA:ARBA00009902, ECO:0000256|RuleBase:RU362110}.
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DR AlphaFoldDB; A0A0E0CDE0; -.
DR STRING; 40149.A0A0E0CDE0; -.
DR EnsemblPlants; OMERI01G41910.1; OMERI01G41910.1; OMERI01G41910.
DR Gramene; OMERI01G41910.1; OMERI01G41910.1; OMERI01G41910.
DR eggNOG; KOG0228; Eukaryota.
DR HOGENOM; CLU_001528_6_0_1; -.
DR Proteomes; UP000008021; Chromosome 1.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004564; F:beta-fructofuranosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd18624; GH32_Fruct1-like; 1.
DR FunFam; 2.115.10.20:FF:000001; Beta-fructofuranosidase, insoluble isoenzyme CWINV1; 1.
DR FunFam; 2.60.120.560:FF:000002; Beta-fructofuranosidase, insoluble isoenzyme CWINV1; 1.
DR Gene3D; 2.60.120.560; Exo-inulinase, domain 1; 1.
DR Gene3D; 2.115.10.20; Glycosyl hydrolase domain, family 43; 1.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR050551; Fructan_Metab_Enzymes.
DR InterPro; IPR001362; Glyco_hydro_32.
DR InterPro; IPR013189; Glyco_hydro_32_C.
DR InterPro; IPR013148; Glyco_hydro_32_N.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR InterPro; IPR007262; Vps55/LEPROT.
DR PANTHER; PTHR31953; BETA-FRUCTOFURANOSIDASE, INSOLUBLE ISOENZYME CWINV1-RELATED; 1.
DR Pfam; PF08244; Glyco_hydro_32C; 1.
DR Pfam; PF00251; Glyco_hydro_32N; 1.
DR Pfam; PF04133; Vps55; 1.
DR SMART; SM00640; Glyco_32; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
PE 3: Inferred from homology;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU362110};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU362110};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000008021};
KW Secreted {ECO:0000256|ARBA:ARBA00022525};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 66..85
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 173..487
FT /note="Glycosyl hydrolase family 32 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF00251"
FT DOMAIN 490..671
FT /note="Glycosyl hydrolase family 32 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF08244"
SQ SEQUENCE 697 AA; 78270 MW; A20163B9044C1FDF CRC64;
MAMTVEGSLD LHLCSQQACA LYNNWWPMLA ALMYVLVPMP CLFFGGGSTQ FLTSRDGGGW
FNAAKFLTGA SAMGSIAIPA ILRHAGLIET GAMFIEFTSF FILVCTVMCF HRATLDEDWK
TPEEVKREDL EKKNLAQIDS SRNGVEATQR VFLYPQSPKV SSIVSKGYRT GYHFQPPKNW
INGPMYYNGI YHEFYQYNPN GSVWGNIVWG HSVSTDLIDW IRLEPAIEGN TPSDVNGCWT
GSATILTGDQ PVIIYTGADT EKRQVQNIVL PKNRSDPYLR EWTKPKNNPL IEPVGPGLNS
NQFRDPTTGW IGPDGLWRIA VGAELNGYSA ALLYKSKDFM QWTRVDHPLY SSNASIMWEC
PDFFAVLPGK NNGLDLSAAI PNGAKHVLKM SLDSCDKYMI GVYDLKHDMF VPDTVLDDRR
LWLRIDYGNY YASKSFFDSK KGRRIIWGWT NETDSTSDDV AKGWAGIHAI PRTIWLDGDG
KRLLQWPIEE VESLRRNEVS HQGLELKKDV EIDFELTSID AADPFDPSWL LDTEKHCREA
DASVHGGLGP FGLIVLASDN MDEHTTVHFR VYKSEQKYMV LLCSDLRRSS LRPGLYTPAY
GGFFEYDLEK EKKISLRTLI DRSAVESFGG GGRACIMARV YPAAVVDGTT HMYAINNGSS
TVKVSQLKAW SMTRAQKGDG NQIIVKNWFA IERFIAA
//
