ID A0A0H2S5R3_9AGAM Unreviewed; 1101 AA.
AC A0A0H2S5R3;
DT 16-SEP-2015, integrated into UniProtKB/TrEMBL.
DT 16-SEP-2015, sequence version 1.
DT 18-JUN-2025, entry version 37.
DE RecName: Full=Isoleucine--tRNA ligase, cytoplasmic {ECO:0000256|ARBA:ARBA00069879};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=SCHPADRAFT_869061 {ECO:0000313|EMBL:KLO16958.1};
OS Schizopora paradoxa.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Hymenochaetales; Schizoporaceae; Schizopora.
OX NCBI_TaxID=27342 {ECO:0000313|EMBL:KLO16958.1, ECO:0000313|Proteomes:UP000053477};
RN [1] {ECO:0000313|EMBL:KLO16958.1, ECO:0000313|Proteomes:UP000053477}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KUC8140 {ECO:0000313|EMBL:KLO16958.1,
RC ECO:0000313|Proteomes:UP000053477};
RG DOE Joint Genome Institute;
RA Min B., Park H., Jang Y., Kim J.-J., Kim K.H., Pangilinan J., Lipzen A.,
RA Riley R., Grigoriev I.V., Spatafora J.W., Choi I.-G.;
RT "Complete genome sequence of Schizopora paradoxa KUC8140, a cosmopolitan
RT wood degrader in East Asia.";
RL Submitted (APR-2015) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP +
CC diphosphate; Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666, Rhea:RHEA-
CC COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58045,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78528, ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00048359};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; KQ085910; KLO16958.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0H2S5R3; -.
DR FunCoup; A0A0H2S5R3; 619.
DR STRING; 27342.A0A0H2S5R3; -.
DR InParanoid; A0A0H2S5R3; -.
DR OrthoDB; 1706657at2759; -.
DR Proteomes; UP000053477; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA deacylase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR FunFam; 1.10.730.10:FF:000004; Isoleucyl-tRNA synthetase, cytoplasmic; 1.
DR FunFam; 3.40.50.620:FF:000133; Isoleucyl-tRNA synthetase, cytoplasmic; 1.
DR FunFam; 3.40.50.620:FF:000023; Isoleucyl-tRNA synthetase,cytoplasmic; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.730.10; Isoleucyl-tRNA Synthetase, Domain 1; 1.
DR Gene3D; 3.90.740.10; Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000053477}.
FT DOMAIN 22..665
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 721..875
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1101 AA; 126857 MW; 6629038282CAADB7 CRC64;
MPTPSGEVQS GQLDFAKEEE RILAYWREID AFQTQLKLSE GRPEFSFYDG PPFATGLPHY
GNLLVGTIKD IVTRHASASG YHVIRRFGWD THGVPVEQEI DKDLNIKSKA DVYALPATET
STGGISTYNE KCRAIVMRYA SEWRTTVERT GRWIDFDNDY KTLDTPFMES VWWAFGQLYE
KGQVYRGRKV MPYSTGLTTP LSNFEAELAF DNIRDPAIVV AFTLKDDPKT SLLAWTTTPW
TLPSNLALCV NPEFRYIKVY DEERGHNFIL FEELLKIVYG DDRKEAKKED SDAKDDKQRY
RKVGEYTGKE MLGWRYVPLF NYFTDRFKDT AFRVLNDSYV SNKEGTGIVH QAPGFGEDDY
RVCLAHGVLE VDDEPPCPLD DVGKFVNEVP DFEGQYFKKV DKEVIGLLKA RGHIIVHSSI
VHKYPLCYRS KTPLLFRVMP VWFVRVKPIV DQLAENNLTT TWVPENVQTG RFGNWLKNAR
DWNISRNRYW GTPIPLWVSE DMQEIKCISS VKQLEELSGV KGITDLHRDK IDHITIPSET
GRGVLKRVEE VFDCWFESGS MPYAQVHYPF ENKERFDSSF PAHFICEGID QTRGWFYTLL
VLATHLFGKA PWRNLIATGL VLAADGKKMS KSLKNYPDPN EILNKYGADA TRMFLVNSSV
TQGENLLFRE EGVRDIIARV LRPWHNSLQF FLQQVAVHKK TGLIPEFVYE ARVKRSENIM
DRWILAKCQS LIELVNKEMA AYRLYTVVTR LLDLVDELTN WYIRFNRRRL KGEDGREDTV
QALNTLFETL FTLCRTMSSF TPFLTESIYQ QLRVFIPDSP NEDTRSIHFL GFPEVKEEYF
DTVIERQVKR MQTIIEQTRA IRDRKMLAFK VPLKELIVFH PDKEYLDDVR PLQRYIESEL
NVIDVKFTSD ESKAGVRYSA VADWPVLGRK LRKDIGRVKA GLTKVTSDEV KKYLETGKIT
VDGIELVAGD LAVRRSAGQL EVGESSKYDT NTDNDVVVLL DVTKYKELEG ETLARELINR
IQKLRKKAGL QATDDVEIYY KFENGVGQDL LDAMKSHGDF IRKTVRAVPK DISERGAGGK
ELINEEQEIL ETKFDLTIVK D
//
