ID A0A0M3JA70_ANISI Unreviewed; 174 AA.
AC A0A0M3JA70;
DT 11-NOV-2015, integrated into UniProtKB/TrEMBL.
DT 11-NOV-2015, sequence version 1.
DT 28-JAN-2026, entry version 33.
DE RecName: Full=GDP-fucose protein O-fucosyltransferase 1 {ECO:0000256|ARBA:ARBA00021745};
DE EC=2.4.1.221 {ECO:0000256|ARBA:ARBA00012196};
DE AltName: Full=Peptide-O-fucosyltransferase 1 {ECO:0000256|ARBA:ARBA00033080};
GN ORFNames=ASIM_LOCUS4301 {ECO:0000313|EMBL:VDK23551.1};
OS Anisakis simplex (Herring worm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Spirurina; Ascaridomorpha; Ascaridoidea; Anisakidae; Anisakis;
OC Anisakis simplex complex.
OX NCBI_TaxID=6269 {ECO:0000313|WBParaSite:ASIM_0000449001-mRNA-1};
RN [1] {ECO:0000313|WBParaSite:ASIM_0000449001-mRNA-1}
RP IDENTIFICATION.
RG WormBaseParasite;
RL Submitted (FEB-2017) to UniProtKB.
RN [2] {ECO:0000313|EMBL:VDK23551.1, ECO:0000313|Proteomes:UP000267096}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RG Pathogen Informatics;
RL Submitted (NOV-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-seryl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-fucosyl)-
CC L-seryl-[protein] + GDP + H(+); Xref=Rhea:RHEA:63644, Rhea:RHEA-
CC COMP:9863, Rhea:RHEA-COMP:17914, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29999, ChEBI:CHEBI:57273, ChEBI:CHEBI:58189,
CC ChEBI:CHEBI:189632; EC=2.4.1.221;
CC Evidence={ECO:0000256|ARBA:ARBA00048647};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63645;
CC Evidence={ECO:0000256|ARBA:ARBA00048647};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=L-threonyl-[protein] + GDP-beta-L-fucose = 3-O-(alpha-L-
CC fucosyl)-L-threonyl-[protein] + GDP + H(+); Xref=Rhea:RHEA:70491,
CC Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:17915, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30013, ChEBI:CHEBI:57273, ChEBI:CHEBI:58189,
CC ChEBI:CHEBI:189631; EC=2.4.1.221;
CC Evidence={ECO:0000256|ARBA:ARBA00047273};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:70492;
CC Evidence={ECO:0000256|ARBA:ARBA00047273};
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC {ECO:0000256|ARBA:ARBA00004922}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000256|ARBA:ARBA00004240}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 65 family.
CC {ECO:0000256|ARBA:ARBA00010626}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; UYRR01007382; VDK23551.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A0M3JA70; -.
DR WBParaSite; ASIM_0000449001-mRNA-1; ASIM_0000449001-mRNA-1; ASIM_0000449001.
DR OrthoDB; 10050276at2759; -.
DR UniPathway; UPA00378; -.
DR Proteomes; UP000267096; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0046922; F:peptide-O-fucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006004; P:fucose metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.11340; -; 1.
DR InterPro; IPR019378; GDP-Fuc_O-FucTrfase.
DR InterPro; IPR039922; POFUT1.
DR PANTHER; PTHR21420; GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 1; 1.
DR PANTHER; PTHR21420:SF10; GDP-FUCOSE PROTEIN O-FUCOSYLTRANSFERASE 1; 1.
DR Pfam; PF10250; O-FucT; 1.
PE 3: Inferred from homology;
KW Carbohydrate metabolism {ECO:0000256|ARBA:ARBA00023277};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Fucose metabolism {ECO:0000256|ARBA:ARBA00023253};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Notch signaling pathway {ECO:0000256|ARBA:ARBA00022976};
KW Reference proteome {ECO:0000313|Proteomes:UP000267096};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..174
FT /note="GDP-fucose protein O-fucosyltransferase 1"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5043120821"
SQ SEQUENCE 174 AA; 20166 MW; C06DAD51302BC0D7 CRC64;
MTFQMSFTNL LLSLLIYSSS EVIGAYEIDP KGYVVYCPCM GRFGNQVEQL LGSISFARAL
NRTLVLPPFI EYIRGQPKKM VDYDEYFQVE PFKQDSLRVI LMRDFIQQIA PRIWPLNRRK
VFCWSAHPSI YNKSLPSSCH AKEGNPFGPF WDYSGIDFIG DIYYGEEINE GFNL
//
