ID A0A139A748_GONPJ Unreviewed; 2352 AA.
AC A0A139A748;
DT 11-MAY-2016, integrated into UniProtKB/TrEMBL.
DT 11-MAY-2016, sequence version 1.
DT 18-JUN-2025, entry version 35.
DE SubName: Full=PROCN-domain-containing protein {ECO:0000313|EMBL:KXS12646.1};
GN ORFNames=M427DRAFT_71935 {ECO:0000313|EMBL:KXS12646.1};
OS Gonapodya prolifera (strain JEL478) (Monoblepharis prolifera).
OC Eukaryota; Fungi; Fungi incertae sedis; Chytridiomycota;
OC Chytridiomycota incertae sedis; Monoblepharidomycetes; Monoblepharidales;
OC Gonapodyaceae; Gonapodya.
OX NCBI_TaxID=1344416 {ECO:0000313|EMBL:KXS12646.1, ECO:0000313|Proteomes:UP000070544};
RN [1] {ECO:0000313|EMBL:KXS12646.1, ECO:0000313|Proteomes:UP000070544}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JEL478 {ECO:0000313|EMBL:KXS12646.1,
RC ECO:0000313|Proteomes:UP000070544};
RX PubMed=25977457; DOI=10.1093/gbe/evv090;
RA Chang Y., Wang S., Sekimoto S., Aerts A.L., Choi C., Clum A., LaButti K.M.,
RA Lindquist E.A., Yee Ngan C., Ohm R.A., Salamov A.A., Grigoriev I.V.,
RA Spatafora J.W., Berbee M.L.;
RT "Phylogenomic analyses indicate that early fungi evolved digesting cell
RT walls of algal ancestors of land plants.";
RL Genome Biol. Evol. 7:1590-1601(2015).
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
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DR EMBL; KQ965786; KXS12646.1; -; Genomic_DNA.
DR STRING; 1344416.A0A139A748; -.
DR OMA; ANKWNTS; -.
DR OrthoDB; 1931567at2759; -.
DR Proteomes; UP000070544; Unassembled WGS sequence.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IEA:TreeGrafter.
DR GO; GO:0071014; C:post-mRNA release spliceosomal complex; IEA:EnsemblFungi.
DR GO; GO:0000974; C:Prp19 complex; IEA:EnsemblFungi.
DR GO; GO:0046540; C:U4/U6 x U5 tri-snRNP complex; IEA:EnsemblFungi.
DR GO; GO:0005682; C:U5 snRNP; IEA:EnsemblFungi.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IEA:EnsemblFungi.
DR GO; GO:0000386; F:second spliceosomal transesterification activity; IEA:EnsemblFungi.
DR GO; GO:0030619; F:U1 snRNA binding; IEA:EnsemblFungi.
DR GO; GO:0030620; F:U2 snRNA binding; IEA:EnsemblFungi.
DR GO; GO:0030623; F:U5 snRNA binding; IEA:EnsemblFungi.
DR GO; GO:0017070; F:U6 snRNA binding; IEA:EnsemblFungi.
DR GO; GO:0000350; P:generation of catalytic spliceosome for second transesterification step; IEA:EnsemblFungi.
DR GO; GO:0000389; P:mRNA 3'-splice site recognition; IEA:EnsemblFungi.
DR GO; GO:0000395; P:mRNA 5'-splice site recognition; IEA:EnsemblFungi.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IEA:EnsemblFungi.
DR CDD; cd08056; MPN_PRP8; 1.
DR CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR FunFam; 1.20.80.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.30.420.230:FF:000003; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.30.43.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.40.140.10:FF:000002; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.90.1570.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR Gene3D; 1.20.80.40; -; 1.
DR Gene3D; 3.30.420.230; -; 1.
DR Gene3D; 3.90.1570.40; -; 1.
DR Gene3D; 3.40.140.10; Cytidine Deaminase, domain 2; 1.
DR Gene3D; 3.30.43.40; Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain; 1.
DR InterPro; IPR000555; JAMM/MPN+_dom.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR012591; PRO8NT.
DR InterPro; IPR012592; PROCN.
DR InterPro; IPR012984; PROCT.
DR InterPro; IPR027652; PRP8.
DR InterPro; IPR021983; PRP8_domainIV.
DR InterPro; IPR043173; Prp8_domainIV_fingers.
DR InterPro; IPR043172; Prp8_domainIV_palm.
DR InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR PANTHER; PTHR11140; PRE-MRNA SPLICING FACTOR PRP8; 1.
DR PANTHER; PTHR11140:SF0; PRE-MRNA-PROCESSING-SPLICING FACTOR 8; 1.
DR Pfam; PF01398; JAB; 1.
DR Pfam; PF08082; PRO8NT; 1.
DR Pfam; PF08083; PROCN; 1.
DR Pfam; PF08084; PROCT; 1.
DR Pfam; PF12134; PRP8_domainIV; 1.
DR Pfam; PF10598; RRM_4; 1.
DR Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR Pfam; PF10596; U6-snRNA_bdg; 1.
DR SMART; SM00232; JAB_MPN; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 2.
DR PROSITE; PS50249; MPN; 1.
PE 4: Predicted;
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000070544};
KW Ribonucleoprotein {ECO:0000256|ARBA:ARBA00023274};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728}.
FT DOMAIN 2110..2241
FT /note="MPN"
FT /evidence="ECO:0000259|PROSITE:PS50249"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..10
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2352 AA; 273391 MW; 37954B6864214A06 CRC64;
MAGLPPSQSY PFAGQVPAAT PMDLDDDKIA AKAKKWAQMN AKRFAEKRKF GGQESQKEMM
PPEHVRKIIK DHGDMSSKKF RHDKRVYLGA LKYVPHAVLK LLENMPMPWE QVREVPVLYH
ITGAITFVNE TPWVIEPVYI AQWATMWMMM RREKRDRRHF KRMRFPPFDD EEPPLDYGDN
VLDVDPLEAI QMELDPNDDA AIFQNFYDHK PWSGTPFVNG PSYRRWKFSL PAMATLHRFA
SQLLSDLVDP NYFYLFDDKA FFTAKALNVA IPGGAKFEPL YRDADPAEED WNEFNDIAKV
IIRQQIRTEY RVAFPHLYNN LPRSVHVAPY HRPAVCYIKT DDPDLPAFYY DPVLAPVSSR
TIQSTGAESH LQELFGDGED DLDEFTLPET VTPFLETTEL YTDNTGPGLA LFWAPHPFDK
RSGRMRRAED VPLVKNWYLE HCPPNQPVKV RVSYQKLLKC HILNRLKHRP PKALNKKFLF
RQLAATKFFQ KTELDWVEAG LQVCRQGYNM LNLLIHRKNL NYLHLDYNFN LKPVKTLTTK
ERKKSRFGNA FHLCREILRL TKLVVDSHVQ YRLGNVDAFQ LADGLQYTFA HVGQLTGMYR
YKYRLMRQIR MCKDLKHVIY YRFNTGPVGK GPGCGFWAPG WRVWLFFLRG VVPLLERWLG
NLLARHFEGR VTKNIAKTVT KQRVESHFDL ELRAAVMADI MDMIPEGIRA NKARTILQHL
SEAWRCWKAN IPWKVPGLPI PIENMVLRYV KAKADWWTSV SHYNRERIRR GATVDKTVVK
KNLGRLTRLY LKAEQERQHN YLKDGPYISP EEAVAIYTTT VHWLESRKFS PIPFPPLSYK
HDTKLLVLAL ERLREAYSVK GRLNASQREE LGLIEQAYDN PHEALSRIKR LLLTQRAFKE
VGIEFMDQYS HIIPVYDIEP LEKITDAYLD QYLWYEGDKR HLFPAWIKPS DSEPPPLLVF
KWCQGINNLT DVWDTSDGQC NVMLETRLAK PYEKIDLVVL NRLLRLIMDH NLADYLTAKN
NVVIAFKDMS HVNAYGLIRG LQFSSFIWQY YGLILDLLIL GLQRASEMAG PPNLPNDFLQ
FRDVATESRH PIRLYARNVD IIHMFFRFTA EEARDLIQRY LTEHPDPNNE NVVGYNNKKV
WPRDCRMRLM KHDVNLGRAV FWEIKNRLPR SLTTIEWDES FCSVYSKDNP NLLFSMVGFD
IRILPKMRSI NEEFTLREGV WNLVNEQTKE RTAQAFLRVD EESLQRFHNR IRQILMSSGS
TTFTKIVNKW NTALIGLMTY YREAVVHTRE LLDLLVKCEN KIQTRVKIGL NSKMPSRFPP
VVFYTPKELG GLGMLSMGHV LIPQSDLRWS KQTDVGITHF RSGMSHEEDQ VIPNLYRYIQ
PWESEFIDSQ RVWAEYALKR QEANAQNRRL TLEDLEDSWD RGIPRINTLF QKDRHTLAYD
KGWRMRTEWK MYQILKNNPF WWTHQRHDGK LWNLNNYRTD VIQALGGVEG ILEHTLFKGT
YFPTWEGLFW EKASGFEESM KYKKLTNAQR SGLNQIPNRR FTLWWSPTIN RANVYVGFQV
QLDLTGIFMH GKIPTLKISL IQIFRAHLWQ KIHESVVMDL CQVFDQELEA LEIETVQKET
IHPRKSYKMN SSCADILLFA AYKWNVSKPS LLSDTKDSFD GATTQKYWVD IQLRWGDFDS
HDVERYTRAK FLDYTTDNMS IYPSPTGVMI AIDLAYNLHS AYGHWFPGMK PLIQQAMAKI
MKANPALYVL RERCRKALQL YSSEPTEPYL NSQNYGELFS NQIIWFVDDT NVYRVTIHKT
FEGNLTTKPI NGAIFIFNPR TGQLFLKIIH TSVWAGQKRL GQLAKWKTAE EVAALIRSLP
IEEQPRQIIV TRKGMLDPLE VHLLDFPNIV IKGSELQLPF QACLKVEKFG DLILKATEPQ
MCLFNLYDDW LKSISSYTAF SRLILILRAL HVNNEKAKIV LRPDKNTITE PHHIWPSLSD
EEWIKAEVAL KDLILADYGK KNNVNVASLT TSEVRDILLG MEIAAPSLQR QQIAEIEKQT
TEQSQLTAIT TRTQNVHGDE MIVTTTTNYE AQTFASKTEW RVRAISSTNL HLRTNHLFVD
SDDIKESGYI HILPKNILKR FITISDLRTQ VAGFMYGVSP PDNSQVKEVR CIVMVPQWGT
HQQVNLPHLL PEHEYLKDLE PLGWIHTQPS MLPQLPPVDV ITHAKILSDN SAWDGEKCIV
VTASFTPGSV SLAAYKLTPS GFEWGRSASL KEGANPQGYL PSHYEKSQIH LSEKFLGYFM
VPDGDGIWNY NFMGAKWRQD MRYSLKLDLP LEFYNELHRP AHFLNFAAVE DEGFGAFGSG
FQGEADREDE FA
//
