ID A0A146FSQ0_ASPKA Unreviewed; 1077 AA.
AC A0A146FSQ0;
DT 08-JUN-2016, integrated into UniProtKB/TrEMBL.
DT 08-JUN-2016, sequence version 1.
DT 28-JAN-2026, entry version 41.
DE RecName: Full=Isoleucine--tRNA ligase, cytoplasmic {ECO:0000256|ARBA:ARBA00069879};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN Name=ILS1 {ECO:0000313|EMBL:BCR97082.1};
GN ORFNames=AKAW2_30401S {ECO:0000313|EMBL:BCR97082.1}, RIB2604_02602580
GN {ECO:0000313|EMBL:GAT28615.1};
OS Aspergillus kawachii (White koji mold) (Aspergillus awamori var. kawachi).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=1069201 {ECO:0000313|EMBL:GAT28615.1, ECO:0000313|Proteomes:UP000075230};
RN [1] {ECO:0000313|EMBL:GAT28615.1, ECO:0000313|Proteomes:UP000075230}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIB 2604 {ECO:0000313|EMBL:GAT28615.1,
RC ECO:0000313|Proteomes:UP000075230};
RX PubMed=27651094;
RA Yamada O., Machida M., Hosoyama A., Goto M., Takahashi T., Futagami T.,
RA Yamagata Y., Takeuchi M., Kobayashi T., Koike H., Abe K., Asai K.,
RA Arita M., Fujita N., Fukuda K., Higa K., Horikawa H., Ishikawa T.,
RA Jinno K., Kato Y., Kirimura K., Mizutani O., Nakasone K., Sano M.,
RA Shiraishi Y., Tsukahara M., Gomi K.;
RT "Genome sequence of Aspergillus luchuensis NBRC 4314.";
RL DNA Res. 23:507-515(2016).
RN [2] {ECO:0000313|Proteomes:UP000075230}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=RIB 2604 {ECO:0000313|Proteomes:UP000075230};
RA Yamada O.;
RT "Genome sequencing of Aspergillus luchuensis NBRC 4314.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:BCR97082.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IFO 4308 {ECO:0000313|EMBL:BCR97082.1};
RG Aspergillus luchuensis mut. kawachii IFO 4304 genome sequencing consortium;
RA Kazuki M., Futagami T.;
RL Submitted (JAN-2021) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EMBL:BCR97082.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=IFO 4308 {ECO:0000313|EMBL:BCR97082.1};
RA Mori K., Kadooka C., Goto M., Futagami T.;
RT "Aspergillus luchuensis mut. kawachii IFO 4304 genome sequence.";
RL Submitted (FEB-2021) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=tRNA(Ile) + L-isoleucine + ATP = L-isoleucyl-tRNA(Ile) + AMP +
CC diphosphate; Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666, Rhea:RHEA-
CC COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58045,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78528, ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00048359};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594, ECO:0000256|RuleBase:RU363035}.
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DR EMBL; AP024427; BCR97082.1; -; Genomic_DNA.
DR EMBL; BCWF01000025; GAT28615.1; -; Genomic_DNA.
DR RefSeq; XP_041540848.1; XM_041686911.1.
DR AlphaFoldDB; A0A146FSQ0; -.
DR GeneID; 64958407; -.
DR KEGG; aluc:AKAW2_30401S; -.
DR VEuPathDB; FungiDB:ASPFODRAFT_129791; -.
DR OrthoDB; 1706657at2759; -.
DR Proteomes; UP000075230; Unassembled WGS sequence.
DR Proteomes; UP000661280; Chromosome 3.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA deacylase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR FunFam; 3.90.740.10:FF:000044; Isoleucine--tRNA ligase; 1.
DR FunFam; 1.10.730.10:FF:000004; Isoleucyl-tRNA synthetase, cytoplasmic; 1.
DR FunFam; 3.40.50.620:FF:000023; Isoleucyl-tRNA synthetase,cytoplasmic; 1.
DR FunFam; 3.40.50.620:FF:000050; Isoleucyl-tRNA synthetase,cytoplasmic; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR Gene3D; 1.10.730.10; Isoleucyl-tRNA Synthetase, Domain 1; 1.
DR HAMAP; MF_02003; Ile_tRNA_synth_type2; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|RuleBase:RU363035};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU363035};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|RuleBase:RU363035};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU363035};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917,
KW ECO:0000256|RuleBase:RU363035};
KW Reference proteome {ECO:0000313|Proteomes:UP000661280}.
FT DOMAIN 12..634
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 691..846
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1077 AA; 124363 MW; E82E18E1D865E0E5 CRC64;
MSIDFPREEE AILQRWREIN AFRRQVELSE GRKPYTFYDG PPFATGLPHY GHLLASTIKD
IIPRYWSMKG HYVERRFGWD THGVPIEYEI DKKLGMSGLE AVEKIGIEKY NEECRAIVMR
FASEWRQTIE RLGRWIDFDN DYKTMNVSFM ESVWWVFKQL FDKDLVYRGY RVMPYSTALN
TPLSNFEAQQ NYKDVQDPAV VVTFPLVHEP ETSLLAWTTT PWTLPSNIAL AVNPTFEYIK
ILDEASGKHY ILLESLLRTL YKDPKKAKFK IVDRFKGATM KDWKYQPLFD YFYEEFKEHG
FRVLNAEYVT AEDGTGIVHQ APAFGEDDYR VGMENGVISE SRLPPNPVDE KGCYTAEVKD
FEGQHVKAAD RGIIKHLKAA GRLVVDSQIT HSYPFCWRSD TPLIYRAVPA WFVKIGPIIP
QMLQGIDDSH WVPSFVKERR FSSWIQNARD WNISRNRFWG TPLPLWVSDD FKEVVAVGSA
EELKQLSGYE GELTDLHRDK VDKITIPSKQ GKGVLRRVSE VFDCWFESGS MPYASQHYPF
ENKEQFENSF PGDFIAEGLD QTRGWFYTLT VLGTHLFGKL PFKNCVVNGI VLAEDGKKMS
KRLKNYPDPA LVMERYGSDA LRLYLINSPV VRAEPLRFKE SGVKEIVAKV LLPLWNSYKF
FEGQAALFKK TQGFDYTFDP KAEATNTNVM DRWILASCQS LLKFVNEEMA GYRLYTVVPR
LLELIDNTTN WYIRFNRRRL KGENGVDDTQ HALNTLFEVL YTLVRGLAPF TPFLTDTIYL
KLLPHIPEAL RSEDSRSVHF LPFPEVREEL FDEVVERRVA RMQKVIDMAR ISRERRNIGL
KTPLKTLVVI HQDQQYLDDV KSLQGYILEE LNVLELVLSS DEEKYNVQYS VTADWPTLGK
KLKKEVQKVK KALPSLTSED VKKFVADKKM LVDGIELVEG DLVVKRGMKE DSASANMEPN
TDSDLLTILD ANLYPELAEQ GLGREIINRL QRLRKKAGLV PTDDVKMEYA VLSDPEDVGI
NKAFETQAKA IEKVVRRPLD RYELVDGKVP SGEEPGMIIQ EEQEVQKATF LLRLLKL
//
