ID A0A164RFB2_9CRUS Unreviewed; 1240 AA.
AC A0A164RFB2;
DT 06-JUL-2016, integrated into UniProtKB/TrEMBL.
DT 06-JUL-2016, sequence version 1.
DT 10-JUN-2026, entry version 34.
DE SubName: Full=Helicase SKI2W {ECO:0000313|EMBL:KZS08607.1};
GN ORFNames=APZ42_027248 {ECO:0000313|EMBL:KZS08607.1};
OS Daphnia magna.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Crustacea; Branchiopoda;
OC Diplostraca; Cladocera; Anomopoda; Daphniidae; Daphnia.
OX NCBI_TaxID=35525 {ECO:0000313|EMBL:KZS08607.1, ECO:0000313|Proteomes:UP000076858};
RN [1] {ECO:0000313|EMBL:KZS08607.1, ECO:0000313|Proteomes:UP000076858}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Xinb3 {ECO:0000313|EMBL:KZS08607.1,
RC ECO:0000313|Proteomes:UP000076858};
RC TISSUE=Complete organism {ECO:0000313|EMBL:KZS08607.1};
RA Gilbert D.G., Choi J.-H., Mockaitis K., Colbourne J., Pfrender M.;
RT "EvidentialGene: Evidence-directed Construction of Genes on Genomes.";
RL Submitted (MAR-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.13;
CC Evidence={ECO:0000256|ARBA:ARBA00047984};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:KZS08607.1}.
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DR EMBL; LRGB01002190; KZS08607.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A164RFB2; -.
DR STRING; 35525.A0A164RFB2; -.
DR PaxDb; 35525-A0A164RFB2; -.
DR OrthoDB; 64767at2759; -.
DR Proteomes; UP000076858; Unassembled WGS sequence.
DR GO; GO:0055087; C:Ski complex; IEA:TreeGrafter.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003724; F:RNA helicase activity; IEA:UniProtKB-EC.
DR GO; GO:0070478; P:nuclear-transcribed mRNA catabolic process, 3'-5' exonucleolytic nonsense-mediated decay; IEA:TreeGrafter.
DR CDD; cd18795; SF2_C_Ski2; 1.
DR FunFam; 3.40.50.300:FF:000354; ATP-dependent RNA helicase SKI2; 1.
DR FunFam; 3.40.50.300:FF:000447; helicase SKI2W isoform X2; 1.
DR FunFam; 1.10.3380.30:FF:000001; Ski2 ATP-dependent RNA helicase; 1.
DR FunFam; 1.10.3380.30:FF:000016; Superkiller viralicidic activity 2-like (S. cerevisiae); 1.
DR Gene3D; 1.10.3380.30; -; 2.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR025696; Beta-barrel_MTR4.
DR InterPro; IPR011545; DEAD/DEAH_box_helicase_dom.
DR InterPro; IPR014001; Helicase_ATP-bd.
DR InterPro; IPR001650; Helicase_C-like.
DR InterPro; IPR048392; MTR4-like_stalk.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR050699; RNA-DNA_Helicase.
DR InterPro; IPR016438; SKI2-like.
DR InterPro; IPR012961; Ski2/MTR4_C.
DR InterPro; IPR040801; Ski2_N.
DR PANTHER; PTHR12131; ATP-DEPENDENT RNA AND DNA HELICASE; 1.
DR PANTHER; PTHR12131:SF1; ATP-DEPENDENT RNA HELICASE SUPV3L1, MITOCHONDRIAL-RELATED; 1.
DR Pfam; PF00270; DEAD; 1.
DR Pfam; PF08148; DSHCT; 1.
DR Pfam; PF00271; Helicase_C; 1.
DR Pfam; PF21408; MTR4-like_stalk; 1.
DR Pfam; PF13234; MTR4_beta-barrel; 1.
DR Pfam; PF17911; Ski2_N; 1.
DR PIRSF; PIRSF005198; Antiviral_helicase_SKI2; 1.
DR SMART; SM00487; DEXDc; 1.
DR SMART; SM01142; DSHCT; 1.
DR SMART; SM00490; HELICc; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51192; HELICASE_ATP_BIND_1; 1.
DR PROSITE; PS51194; HELICASE_CTER; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000313|EMBL:KZS08607.1};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000076858};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884}.
FT DOMAIN 300..456
FT /note="Helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51192"
FT DOMAIN 551..753
FT /note="Helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51194"
FT REGION 506..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1240 AA; 139309 MW; 5979DA5F04052D8C CRC64;
MDGLPFGAPP ILLDMKSEIQ NYLENPQLLP IHDLEVVQQY WKHDTNIKQL CQAEVAATPT
TLLVERDINT GALAEVVEIV TPHAGMTNKT SMSLQRIPGP PSEWVRGNAS NVPFWPGGLE
WNPSETMSDL LQIDDLMEEL CFKKDLKTVP PGFKNGMNFG TSDVNLNLAA SLKQKAKPHD
TEDKLKTESD SSSVIDLADL LKEKGSIFKW IEEEPVPFQN HKLAEKKSIV MSENEDGDLD
DIPVVDISKV LSLSEKQKLK STDWAEIIDV STSLDNFNDL VPNPAFTWPF ELDRFQKHAI
IHLEKGEDVF IAAHTSAGKT VVAEYAIALS QKHMTRAIYT SPIKALSNQK FRDFKTTFTD
VGLLTGDVQI NAKATCLIMT TEILRSMLYN GSDIIRDLEW VIFDEVHYIN DSERGVVWEE
VLILLPSHVN IVMLSATVPN TSEFATWVGR TKGRKMYVIS TLKRPVPLEH YLYTGLTGKT
KDERFLIVNA EGAFVPKGYM AAMEAKKSKE KDVKPSSAGA GRGRGAPTKE VSRAPGGGGH
KGWGPPQEKN LMIALLDHLK KHDQLPVVAF TFSRNRCDQN SSLLTSVDLV TAEERGRIHQ
FFQKCVSRLK GSDQKLPQVT NMQILLKRGI GVHHSGILPI LKEVVEMLFQ EGLVKLLFAT
ETFAMGVNMP ARTVVFDSIR KHDGTGFRNL LPAEYIQMAG RAGRRGLDTT GTVIILCKND
VPESSELHAM MLGQPMKLSS QFRVTYSMIL NLLRVEHLRV EDMMKRSFGE DHQQSKLGKV
KEQLQKLYDQ VQQLPQLACD ICIDIESYYN NAASYLNSKE EMQESLLTHP SMMREMNPGR
VLIVQHQGRC NKLAVLLSID SRSKEKLYKV LVLISGDGEP TSKKDDMIWT KMLGISQLKK
GLFYPTSRVN HAVVLLKAKQ IWEVTRVQLK IESDKIVADW DNRQIPRFRD NPPGPSCTAA
VQELSRLSQT ASVEVINPLQ DWKWTNMDLV GKMQELSILF NRLASATCTA CSQFEQHLEQ
TSASMSIQEE LQRTQFLLSE DSLLHSADYH SRLEVLKELN YVDGNGTLQM KGKVACEMGN
HELIITELVF HNVLTELQPA EIAALLSCLV FQQKNASEPH LTPVLEKGRL RIREIAEKIG
RTQQACGLKE AVGDFVDQFR FELVEVVYEW AKGMPFAEIM GLTDVQEGMI VRCIQRLDET
LRDVRDAARI IGDPILYQKM GEASTAIKRD IVFAASLYTQ
//
