ID A0A199VG16_ANACO Unreviewed; 889 AA.
AC A0A199VG16;
DT 05-OCT-2016, integrated into UniProtKB/TrEMBL.
DT 05-OCT-2016, sequence version 1.
DT 28-JAN-2026, entry version 34.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=ACMD2_09776 {ECO:0000313|EMBL:OAY75725.1};
OS Ananas comosus (Pineapple) (Ananas ananas).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Bromeliaceae;
OC Bromelioideae; Ananas.
OX NCBI_TaxID=4615 {ECO:0000313|EMBL:OAY75725.1, ECO:0000313|Proteomes:UP000092600};
RN [1] {ECO:0000313|EMBL:OAY75725.1, ECO:0000313|Proteomes:UP000092600}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. MD2 {ECO:0000313|Proteomes:UP000092600};
RC TISSUE=Leaf {ECO:0000313|EMBL:OAY75725.1};
RX PubMed=27374615; DOI=10.1093/dnares/dsw026;
RA Redwan R.M., Saidin A., Kumar S.V.;
RT "The draft genome of MD-2 pineapple using hybrid error correction of long
RT reads.";
RL DNA Res. 0:0-0(2016).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OAY75725.1}.
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DR EMBL; LSRQ01002021; OAY75725.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A199VG16; -.
DR STRING; 4615.A0A199VG16; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000092600; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd16655; RING-Ubox_WDSUB1-like; 1.
DR CDD; cd14066; STKc_IRAK; 1.
DR CDD; cd01989; USP_STK_Ubox_N; 1.
DR FunFam; 3.30.200.20:FF:000039; receptor-like protein kinase FERONIA; 1.
DR FunFam; 3.30.40.10:FF:000428; U-box domain-containing protein 54; 1.
DR Gene3D; 1.10.287.2610; -; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.30.200.20; Phosphorylase Kinase, domain 1; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR051348; U-box_ubiquitin_ligases.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR45647; OS02G0152300 PROTEIN; 1.
DR PANTHER; PTHR45647:SF100; U-BOX DOMAIN-CONTAINING PROTEIN 33; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00220; S_TKc; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Coiled coil {ECO:0000256|SAM:Coils};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT DOMAIN 527..794
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT DOMAIN 814..888
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
FT REGION 261..322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 353..506
FT /evidence="ECO:0000256|SAM:Coils"
FT BINDING 555
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 889 AA; 98885 MW; 43015164B8C3EE56 CRC64;
MEGNRSWETA SNRSGRSSSA SLAAAAAAAA AAEGEERVYV AVAEEIKYGK NTLLWALQNV
SKDARIVIAH VHVPAQMIPF MGAKFHASKL KPQEVAAYRQ LEREKMVKSL DQYLVVCSKV
KVKAEKLVIE MDDVAKGLLE LISLHGITKL VMGAAADKHY SKKMNTPKSK TAIAVLQKAD
PSCKIWFVCK GNLIYTKEAG FDAPGIAPSP FSSSSSMSSL SDRLRSLTLP QGQGSTPLSS
SQGSIRQDYF TQRSRNDTLA ALPAGLHPRG PDQVTLDRRL STNSSIDPWE GISRRTSRTS
SSTSDEVVSN LGPVQLAREE ESEVGSVVLP SVHESEEDHF SSPRHELDDM GLDADVYDKL
REALAEAENL KNEAFEESRR RRKAEKDVIL ALQKVKESEN LYKKEAKRRK EIEESLAKEM
QEIELLKNQR DEIAEQLQKA NEQMLALEQQ ITSSDYATKD LEEKLSAARD LLHSLQAEFD
NLQRERDDAV KEAEELRKKR EQMSSDSHGA LNSEFSLLEL KQATQNFCET LKIGEGGFGS
VYKGFLRNTT VAIKKLHPES LQGQSEFQQE VAVLSRVRHP NLVTLIGACS EASGLVYEYL
PNGSLEDRLV CLNNTPPLTW QVRIRIIGEI CSALIFLHSN KPHPVVHGDL KPSNILLDAN
FISKLSDFGI CRLLIQSNTT TTTLYRTTNP RGTFAYMDPE FISTGQLTPR SDIYSFGIII
LRLLTGKPPL GIAREVEDAV EDGNLHSMID STAGDWPFVQ AKQLAHIGLR CAELSRRRRP
DLVEEVWRVV EPLMKAASLS ASPPPSGSVL DEHRIPSYFV CPIFQEIMRD PHIAADGFTY
EAEAIKGWLD SGHDTSPMTN LKLPHCELIP NHALRSAIQE WLQQMQLQL
//
