ID A0A1I7HJ14_9FLAO Unreviewed; 786 AA.
AC A0A1I7HJ14;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 18-JUN-2025, entry version 26.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00044770};
DE EC=2.4.99.28 {ECO:0000256|ARBA:ARBA00044770};
GN ORFNames=SAMN05216480_109137 {ECO:0000313|EMBL:SFU60750.1};
OS Pustulibacterium marinum.
OC Bacteria; Pseudomonadati; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Pustulibacterium.
OX NCBI_TaxID=1224947 {ECO:0000313|EMBL:SFU60750.1, ECO:0000313|Proteomes:UP000199138};
RN [1] {ECO:0000313|EMBL:SFU60750.1, ECO:0000313|Proteomes:UP000199138}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CGMCC 1.12333 {ECO:0000313|EMBL:SFU60750.1,
RC ECO:0000313|Proteomes:UP000199138};
RA de Groot N.N.;
RL Submitted (OCT-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans,octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.99.28;
CC Evidence={ECO:0000256|ARBA:ARBA00049902};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004752}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the transpeptidase
CC family. {ECO:0000256|ARBA:ARBA00007090}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the
CC glycosyltransferase 51 family. {ECO:0000256|ARBA:ARBA00007739}.
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DR EMBL; FPBK01000009; SFU60750.1; -; Genomic_DNA.
DR RefSeq; WP_394331590.1; NZ_FPBK01000009.1.
DR AlphaFoldDB; A0A1I7HJ14; -.
DR STRING; 1224947.SAMN05216480_109137; -.
DR Proteomes; UP000199138; Unassembled WGS sequence.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:TreeGrafter.
DR GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:InterPro.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR050396; Glycosyltr_51/Transpeptidase.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR011815; PBP_1c.
DR InterPro; IPR009647; PBP_C.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR NCBIfam; TIGR02073; PBP_1c; 1.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF15; PENICILLIN-BINDING PROTEIN 1C; 1.
DR Pfam; PF06832; BiPBP_C; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 3: Inferred from homology;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022670};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000199138};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 62..226
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 305..564
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
FT DOMAIN 693..779
FT /note="Penicillin-binding C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06832"
SQ SEQUENCE 786 AA; 88349 MW; DF9BCE52582C5B8E CRC64;
MSMAPLSTKA FVKRNRIKLI GLTLLLIAYY CCLPQPLFTT PTATVIESSD GTLLGAKIAS
DGQWRFPKMD TVPEKFKQCI VQFEDAYFYQ HPGINPVSMG KALVSNIKAG KTVRGGSTLT
QQVIRLSRKG TSRTYLEKLW ESTLATRLEF RYSKDEILNF YASYAPFGGN VVGLDMASWR
YFGVLPQQLS WAESATLAVL PNAPSIIYPG KNQETLHQKR DQLLLKLKNE GIIDDTTYQL
ALAEDLPQQA FPIPRTASHL TEYIAKKHKG ERLQTTIDQK LQHQVNTLVA QHHRKLKQNH
VYNASVLVLD VATRNVLAYV GNAPTDNAHE KDVDMMHAPR STGSVLKPLL YAAMLDNGEL
LPQMLIPDVP TEIAGYQPEN FDESYSGAVP ADEALARSLN IPAVRLLQRH GLEKFRDELD
AFHLKNINKP ANHYGLTLIL GGAESNLWDL CKTYASLAAT LNHFNETSSE YYTDEFQEPN
VYASQKKDFG SKSTQKTVFN AGSIYSVFEA MKQVNRPDSE VSWEYFDSSR EIAWKTGTSF
GNKDAWAIGV NKKYVVGVWV GNADGEGRPQ ITGTSSAAPL LFDVFNVLPK SSWFEMPYDE
MMEVRVCAQS GYLASDICPA KTQWVPKAGA HANLCHYHHT IHVDTQHQFR VNSSCETVDQ
IAHVSWFTLP PLMEFFYKKT HANYKTLPPF RTDCTTSEEK MMEFIYPKDY STITLAKDFD
GKINELVVKV AHRKPATTLL WYLDDSFLKQ TTQFHEVSIN PPTGQHLITV TDIDGNTIAS
HIIINK
//
