ID A0A1K0G0Q3_9BASI Unreviewed; 2391 AA.
AC A0A1K0G0Q3;
DT 15-FEB-2017, integrated into UniProtKB/TrEMBL.
DT 15-FEB-2017, sequence version 1.
DT 02-APR-2025, entry version 28.
DE SubName: Full=Probable PRP8 - U5 snRNP protein, pre-mRNA splicing factor {ECO:0000313|EMBL:SYW82370.1};
DE SubName: Full=Probable PRP8-U5 snRNP protein, pre-mRNA splicing factor {ECO:0000313|EMBL:SAM79997.1};
GN ORFNames=UBRO2_04492 {ECO:0000313|EMBL:SYW82370.1}, UBRO_02279
GN {ECO:0000313|EMBL:SAM79997.1};
OS Ustilago bromivora.
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Ustilago.
OX NCBI_TaxID=307758 {ECO:0000313|EMBL:SAM79997.1, ECO:0000313|Proteomes:UP000179920};
RN [1] {ECO:0000313|EMBL:SAM79997.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=UB2112 {ECO:0000313|EMBL:SAM79997.1};
RA Evans L.H., Alamgir A., Owens N., Weber N.D., Virtaneva K., Barbian K.,
RA Babar A., Rosenke K.;
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000179920}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=UB2112 {ECO:0000313|Proteomes:UP000179920};
RA Guldener U., Guldener U.;
RL Submitted (APR-2016) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:SYW82370.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=UB2 {ECO:0000313|EMBL:SYW82370.1};
RA Guldener U.;
RL Submitted (AUG-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; LT558120; SAM79997.1; -; Genomic_DNA.
DR EMBL; ULHB01000106; SYW82370.1; -; Genomic_DNA.
DR OrthoDB; 1620at5257; -.
DR Proteomes; UP000179920; Chromosome iv.
DR Proteomes; UP000658997; Unassembled WGS sequence.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IEA:TreeGrafter.
DR GO; GO:0005682; C:U5 snRNP; IEA:TreeGrafter.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IEA:TreeGrafter.
DR GO; GO:0030619; F:U1 snRNA binding; IEA:TreeGrafter.
DR GO; GO:0030620; F:U2 snRNA binding; IEA:TreeGrafter.
DR GO; GO:0030623; F:U5 snRNA binding; IEA:InterPro.
DR GO; GO:0017070; F:U6 snRNA binding; IEA:InterPro.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IEA:TreeGrafter.
DR CDD; cd08056; MPN_PRP8; 1.
DR CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR FunFam; 1.20.80.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.30.420.230:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.40.140.10:FF:000002; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.90.1570.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR Gene3D; 1.20.80.40; -; 1.
DR Gene3D; 3.30.420.230; -; 1.
DR Gene3D; 3.90.1570.40; -; 1.
DR Gene3D; 3.40.140.10; Cytidine Deaminase, domain 2; 1.
DR Gene3D; 3.30.43.40; Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain; 1.
DR InterPro; IPR000555; JAMM/MPN+_dom.
DR InterPro; IPR012591; PRO8NT.
DR InterPro; IPR012592; PROCN.
DR InterPro; IPR012984; PROCT.
DR InterPro; IPR027652; PRP8.
DR InterPro; IPR021983; PRP8_domainIV.
DR InterPro; IPR043173; Prp8_domainIV_fingers.
DR InterPro; IPR043172; Prp8_domainIV_palm.
DR InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR PANTHER; PTHR11140; PRE-MRNA SPLICING FACTOR PRP8; 1.
DR PANTHER; PTHR11140:SF0; PRE-MRNA-PROCESSING-SPLICING FACTOR 8; 1.
DR Pfam; PF01398; JAB; 1.
DR Pfam; PF08082; PRO8NT; 1.
DR Pfam; PF08083; PROCN; 1.
DR Pfam; PF08084; PROCT; 1.
DR Pfam; PF12134; PRP8_domainIV; 1.
DR Pfam; PF10598; RRM_4; 1.
DR Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR Pfam; PF10596; U6-snRNA_bdg; 1.
DR SMART; SM00232; JAB_MPN; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 2.
PE 4: Predicted;
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000658997};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728}.
FT DOMAIN 2146..2280
FT /note="JAB1/MPN/MOV34 metalloenzyme"
FT /evidence="ECO:0000259|SMART:SM00232"
FT REGION 1..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 8..26
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 27..37
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2391 AA; 273649 MW; 115E381CE4B5A6CB CRC64;
MSGSPGYRGP PPSGPSSMRG PPPGMPPSAV ASVSSSYRDP AVLTPGQLQA KARKWQQTQS
RRFGDRRGKT GFVDTGKQEM PPEVLRKIIK EHGDMTNRKY RQDKRVHLGA LKYLPHAMMK
VLENIPMPWE QIREVPVLYH ITGAITFVNE TPKVIEPVFH AQWATMWIAM RREKRDRRHF
KRMRFPPFDD EEPVVDYSDN LLDVEPLEAI QLELDQEEDG AVIDWFYDHK PLIDNRKHLN
GSSYRFWNLD LPQMATLHRL GRTLLSDGST SGDRNSFYLF DKKAFFTAKA LNMAIPGGPK
FEPLFKDTDG FDDDLTDFTD FRKLIIRQQI RTEYRVAFPH LYNSRPRAVH IGTYHEPKNV
YIRSDEIDQA FYFDPAINPI SGRTLASSNA FRSEYDDGTP VLSHEDQVFG PDVEGDDELP
DEIAFSLPEE IEPFLEDAPL ENDLTADAIA LWWVPKPYSL RSGRTRRAVD VPLIQPWYLE
HCPPSQPVKV RVSYQKLLKN YVYNSLHTTK QKVHKRTNLL RELNNTKFFQ NTSIDWLEAG
LQVAKQSFNM MNLLIHRKGA SYLHLDYNMN LKPTKTLTTK ERKKSRFGNA FHLVRELSRV
MKLVVDAHVQ FRLGSIDAFQ LADGLQYLFN HVATLSGIYR YKYRSMRQIR ACKDLKHVIY
YRFNAAGVGK GPGVGFWAPS WRVWIFFLRG IVPLLERWLG NLLARQFEGR AGRTGAQSIT
KQRVESAMDL ELRASVMHDI LDAMPEGMRQ NKIKTIMSHL QEAWRACRSN TPWSPNLPAP
IENLILRYVK QKFDWWTSVA HQQRARIARG ATVDKTAVRK NQGRLTRLFL RAEQERQADY
LKSGPYITSE VGVAAFTATV QWLEARRFSP IPFPSLNNKH DVKHLVLALD SLKEAYSVKG
RLNQSQREEL ALIEQAFDNP HETLARIKRL MLTQRAFKEA GFEFFDTFSK LTPTYDIEPI
EKITDAYLSQ YLSYEADKRQ LFPAWIKPSD SEPAPLLVYK WCNGINNAHE IWDTSEGQCN
VLMETTLSKV FEKIDLTLLN RLLRLIMDHN LADYITAKNN ISIVFKDMSH VNSYGLIRGL
QFSAFVFQYY GLVLDLLILG LQRANEMAGP PNMPNGLFQF QNTATETRHP VRLYTRYIDR
IHILYRFTAE EARDLVQRYL TANPDPNNGN VIGYNNRKCW PRDARMRLVK HDVNLGRAVF
WTVKNSLPRS LTTIEWEDTF CSVYSKDNPN LLFSMLGFEV RILPKIRQED VDVARDGVWP
LIQASSGERT STAFLRVSEE GIANFSNRVR ALLMSAGAAP FSKIANKWNT CLIALMTYYR
EAVVNTEDML DLLVRSENKV INRIKSGVNS KMPSRWPIHI LASPKELGGL GMLSMGHVLI
PASDLKYSRQ TTTGITHFRA GLSNQEDQLL PALYRYITPW ASEFEDSARV WQEYAQKRKE
ANEQNRRLTL EDLEDSFHRG LPRIATLFQK DRTILSYDKL WRVRQEFKKY QIAKAQPFAW
TNQRHDGKLH NLNQLRTDTI AALGGVETIL EHSLFAATGH PTWEGLFWEK SSSFEESMQQ
KRLTNAQRSG LSQIPNRRFT LWWSPTLNRS SVYIGFQVQL DLCGVLMSGK MPSLKISYIQ
LFRAHLWQKI HESVVMDLCQ VFDQELEALS IETVQKETIH PRKSYRLTAS SSDILLFASY
KWPMSRPSLL TDSRDVMDGS SGNKWWVDVQ LRWGDYDSHD IERYCRAKFL DYTSDNTSIY
PSATGALVGI DLAYNIHSAY GIWFPGSKPL IQQAMAKIMK ANPALYVLRE RVRKSLQLYS
SEPTEPYLNS NNYAELFSNQ IIWFVDDTNV YRVTIHRTFE GNLVTKPING AIIILNPRTG
QLFLKIIHTS VWAGQKRLGQ LAKWKTAEET IALVRSLPVE EQPKRIIVSR KGMLDPLETH
SLDFPNLSIV GSELQLPFQS FLKLEAIGDL VLKATQPQMV LYSSYDNWMS NVSSFTAFSR
LILLLRSLHV NQEKAKIILR PHPGVITEPH HLWPTLTDEE WIKVEIQLRD LILADFGKRN
NVNVSSLTSS EIRDIILGMT IQAPSVQRQQ MAELEKTAEA AQQVTATQTR TVNVHGEEIQ
TVTTTQYENK VFSSKSDWRV RALSATNLPL RCQHLYVSND NIKDDVGSLT YVMPKNILKT
FIVNADLRTQ VAGYMYGVSP PDAPSIKEIK VIVWVPQRGS NHRVELPDDL PQHDFILKDL
EPLGWIKTSS IDLAHLPPQD VAQHARTMAA HTSWGASTIC ITCAFTPGSV SLSAYNLTVA
GFEWGRKATD TEIAAGGGRN FNVGTMMDRV QLLLSDRILG TTLTPSEGAW NYGLSLSAQW
TSSIKYRVVL DRPAGFWDQV HRPQNFLTFT NAVEETTADT GEVADIQDAF A
//
