ID   A0A1M5YGE9_9FIRM        Unreviewed;       684 AA.
AC   A0A1M5YGE9;
DT   15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT   15-MAR-2017, sequence version 1.
DT   28-JAN-2026, entry version 25.
DE   RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE            EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE   AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE   AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE   AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE   AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN   ORFNames=SAMN02745180_02178 {ECO:0000313|EMBL:SHI10979.1};
OS   Sporanaerobacter acetigenes DSM 13106.
OC   Bacteria; Bacillati; Bacillota; Tissierellia; Tissierellales;
OC   Sporanaerobacteraceae; Sporanaerobacter.
OX   NCBI_TaxID=1123281 {ECO:0000313|EMBL:SHI10979.1, ECO:0000313|Proteomes:UP000184389};
RN   [1] {ECO:0000313|EMBL:SHI10979.1, ECO:0000313|Proteomes:UP000184389}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DSM 13106 {ECO:0000313|EMBL:SHI10979.1,
RC   ECO:0000313|Proteomes:UP000184389};
RA   Jaros S., Januszkiewicz K., Wedrychowicz H.;
RL   Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC         passage and rejoining.; EC=5.6.2.1;
CC         Evidence={ECO:0000256|ARBA:ARBA00000213};
CC   -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC       {ECO:0000256|ARBA:ARBA00009446}.
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DR   EMBL; FQXR01000011; SHI10979.1; -; Genomic_DNA.
DR   RefSeq; WP_072744821.1; NZ_FQXR01000011.1.
DR   AlphaFoldDB; A0A1M5YGE9; -.
DR   STRING; 1123281.SAMN02745180_02178; -.
DR   OrthoDB; 9803554at2; -.
DR   Proteomes; UP000184389; Unassembled WGS sequence.
DR   GO; GO:0043597; C:cytoplasmic replication fork; IEA:TreeGrafter.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006310; P:DNA recombination; IEA:TreeGrafter.
DR   GO; GO:0006281; P:DNA repair; IEA:TreeGrafter.
DR   GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR   CDD; cd00186; TOP1Ac; 1.
DR   CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR   Gene3D; 3.40.50.140; -; 1.
DR   Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR   Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR   Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR   InterPro; IPR000380; Topo_IA.
DR   InterPro; IPR003601; Topo_IA_2.
DR   InterPro; IPR023406; Topo_IA_AS.
DR   InterPro; IPR013497; Topo_IA_cen.
DR   InterPro; IPR013824; Topo_IA_cen_sub1.
DR   InterPro; IPR013825; Topo_IA_cen_sub2.
DR   InterPro; IPR013826; Topo_IA_cen_sub3.
DR   InterPro; IPR023405; Topo_IA_core_domain.
DR   InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR   InterPro; IPR005738; TopoIII.
DR   InterPro; IPR025589; Toprim_C_rpt.
DR   InterPro; IPR006171; TOPRIM_dom.
DR   InterPro; IPR034144; TOPRIM_TopoIII.
DR   NCBIfam; NF005829; PRK07726.1; 1.
DR   NCBIfam; TIGR01056; topB; 1.
DR   PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR   PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR   Pfam; PF01131; Topoisom_bac; 1.
DR   Pfam; PF01751; Toprim; 1.
DR   Pfam; PF13342; Toprim_Crpt; 1.
DR   PRINTS; PR00417; PRTPISMRASEI.
DR   SMART; SM00437; TOP1Ac; 1.
DR   SMART; SM00436; TOP1Bc; 1.
DR   SMART; SM00493; TOPRIM; 1.
DR   SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR   PROSITE; PS00396; TOPO_IA_1; 1.
DR   PROSITE; PS52039; TOPO_IA_2; 1.
DR   PROSITE; PS50880; TOPRIM; 1.
PE   3: Inferred from homology;
KW   DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW   Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:SHI10979.1};
KW   Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW   Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW   Reference proteome {ECO:0000313|Proteomes:UP000184389};
KW   Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT   DOMAIN          1..134
FT                   /note="Toprim"
FT                   /evidence="ECO:0000259|PROSITE:PS50880"
FT   DOMAIN          151..574
FT                   /note="Topo IA-type catalytic"
FT                   /evidence="ECO:0000259|PROSITE:PS52039"
SQ   SEQUENCE   684 AA;  78779 MW;  76B3BDC7140EB4AB CRC64;
     MKLIITEKPS VAMSIAKVLG VHGRKDGYIE SKEYIISWCV GHLVGLAQAD KYNEKYKKWR
     YEDLPIIPSK FQYDLFVKTK KQYEILNKLL NRSDVLEVIN ACDSGREGEL IFRLVYYHSK
     CQKPMKRLWI SSMEDSAIED GFKNLKDGKE FNNLYKSALA RSQADWIVGI NATRLFTVLY
     NQLLSVGRVQ TPTLALIVNR EEDIRSFRKE KFYHLDLYLD GFMASSERIK EKDEANELKN
     LCIGEMAIAK DVKKEKKNVN PPLPFDLTSL QREANRLFGY TAKQTLDYTQ SLYEKKWVTY
     PRTDSRYLTD DMMESLVNLI NGIDRDNLVE NPNYKRILDS KKVTDHHAII PTIQSLNMNI
     EDIPKTEKNI YKLICLKLLE AVADTYVEEI VRVSLLVGDK IFTAKGKKVV NLGFKGIKSS
     FDENFNTMEK EKDNSILLPE IEEGMVFEIQ NSEIREGFTQ SPKHFTEDTL LSAMERAGNE
     ELDKELDVEK KGLGTPATRA GIIEKLISVG YLERKKKQLL PTDKGVSLIT VMPDNLKSPK
     LTAEWENKLT EISLGKGSDE EFLKGIDEMI TDLVKTYSHI AVENKEKFQS NREVLGSCPR
     CKSYIFEGKK NFYCSNKECG FSLLKEDRFF TDKRKKLTKE MVKTLLSKGE VIVKGLYSKK
     TGKTYNAYIS IEDTGKYINY KMRF
//