ID A0A1P6BLD8_BRUMA Unreviewed; 634 AA.
AC A0A1P6BLD8; A0A4E9FK99;
DT 15-MAR-2017, integrated into UniProtKB/TrEMBL.
DT 15-MAR-2017, sequence version 1.
DT 28-JAN-2026, entry version 39.
DE RecName: Full=Vesicle-fusing ATPase {ECO:0000256|RuleBase:RU367045};
DE EC=3.6.4.6 {ECO:0000256|RuleBase:RU367045};
GN Name=Bma-nsf-1 {ECO:0000313|EMBL:CDP93919.1,
GN ECO:0000313|WBParaSite:Bm13759b.1};
GN ORFNames=BM_BM13759 {ECO:0000313|EMBL:VIO95888.1}, BM_Bm13759
GN {ECO:0000313|EMBL:CDP93919.1};
OS Brugia malayi (Filarial nematode worm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Spirurina; Spiruromorpha; Filarioidea; Onchocercidae; Brugia.
OX NCBI_TaxID=6279 {ECO:0000313|Proteomes:UP000006672, ECO:0000313|WBParaSite:Bm13759b.1};
RN [1] {ECO:0000313|EMBL:CDP93919.1, ECO:0000313|Proteomes:UP000006672}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FR3 {ECO:0000313|EMBL:CDP93919.1,
RC ECO:0000313|Proteomes:UP000006672};
RX PubMed=17885136; DOI=10.1126/science.1145406;
RA Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E.,
RA Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T.,
RA Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L.,
RA Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M.,
RA Pop M., White O., Barton G.J., Carlow C.K., Crawford M.J., Daub J.,
RA Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F.,
RA Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.W.,
RA Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M.,
RA McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J.,
RA Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E.,
RA Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G.,
RA Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B.,
RA Blaxter M.L., Scott A.L.;
RT "Draft genome of the filarial nematode parasite Brugia malayi.";
RL Science 317:1756-1760(2007).
RN [2] {ECO:0000313|EMBL:CDP93919.1}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=FR3 {ECO:0000313|EMBL:CDP93919.1};
RG WormBase Consortium;
RA Ghedin E., Paulini M.;
RL Submitted (DEC-2012) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:VIO95888.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FR3 {ECO:0000313|EMBL:VIO95888.1};
RA Howe K., Paulini M., Williams G.;
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|WBParaSite:Bm13759b.1}
RP IDENTIFICATION.
RG WormBaseParasite;
RL Submitted (DEC-2019) to UniProtKB.
CC -!- FUNCTION: Required for vesicle-mediated transport. Catalyzes the fusion
CC of transport vesicles within the Golgi cisternae. Is also required for
CC transport from the endoplasmic reticulum to the Golgi stack. Seems to
CC function as a fusion protein required for the delivery of cargo
CC proteins to all compartments of the Golgi stack independent of vesicle
CC origin. {ECO:0000256|RuleBase:RU367045}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=3.6.4.6;
CC Evidence={ECO:0000256|RuleBase:RU367045};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|RuleBase:RU367045};
CC Note=Binds 1 Mg(2+) ion per subunit. {ECO:0000256|RuleBase:RU367045};
CC -!- SUBUNIT: Homohexamer. {ECO:0000256|ARBA:ARBA00011643}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|RuleBase:RU367045}.
CC -!- SIMILARITY: Belongs to the AAA ATPase family.
CC {ECO:0000256|ARBA:ARBA00006914, ECO:0000256|RuleBase:RU003651}.
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DR EMBL; LN856914; CDP93919.1; -; Genomic_DNA.
DR EMBL; CAAKNF010000194; VIO95888.1; -; Genomic_DNA.
DR RefSeq; XP_042935986.1; XM_043080052.1.
DR AlphaFoldDB; A0A1P6BLD8; -.
DR GeneID; 6100329; -.
DR WBParaSite; Bm13759b.1; Bm13759b.1; WBGene00234020.
DR CTD; 6100329; -.
DR OrthoDB; 9982946at2759; -.
DR Proteomes; UP000006672; Unassembled WGS sequence.
DR GO; GO:0005795; C:Golgi stack; IEA:TreeGrafter.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0043001; P:Golgi to plasma membrane protein transport; IEA:TreeGrafter.
DR GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IEA:TreeGrafter.
DR GO; GO:0035494; P:SNARE complex disassembly; IEA:InterPro.
DR CDD; cd19504; RecA-like_NSF-SEC18_r1-like; 1.
DR FunFam; 1.10.8.60:FF:000026; vesicle-fusing ATPase isoform X1; 1.
DR FunFam; 3.40.50.300:FF:000166; vesicle-fusing ATPase isoform X1; 1.
DR FunFam; 3.40.50.300:FF:000187; Vesicular-fusion ATPase SEC18; 1.
DR Gene3D; 1.10.8.60; -; 2.
DR Gene3D; 3.10.330.10; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR041569; AAA_lid_3.
DR InterPro; IPR003959; ATPase_AAA_core.
DR InterPro; IPR003960; ATPase_AAA_CS.
DR InterPro; IPR029067; CDC48_domain_2-like_sf.
DR InterPro; IPR054419; NSF_ATPase_lid.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR039812; Vesicle-fus_ATPase.
DR PANTHER; PTHR23078:SF3; VESICLE-FUSING ATPASE; 1.
DR PANTHER; PTHR23078; VESICULAR-FUSION PROTEIN NSF; 1.
DR Pfam; PF00004; AAA; 2.
DR Pfam; PF17862; AAA_lid_3; 1.
DR Pfam; PF21964; NSF_ATPase_lid; 1.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF54585; Cdc48 domain 2-like; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 2.
DR PROSITE; PS00674; AAA; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU003651};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|RuleBase:RU367045};
KW ER-Golgi transport {ECO:0000256|RuleBase:RU367045};
KW Hydrolase {ECO:0000256|RuleBase:RU367045};
KW Magnesium {ECO:0000256|RuleBase:RU367045};
KW Metal-binding {ECO:0000256|RuleBase:RU367045};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU003651};
KW Protein transport {ECO:0000256|ARBA:ARBA00022927,
KW ECO:0000256|RuleBase:RU367045};
KW Reference proteome {ECO:0000313|Proteomes:UP000006672};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU367045}.
FT DOMAIN 143..290
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 426..562
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT COILED 568..595
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 634 AA; 70039 MW; 3839462F57F7E821 CRC64;
MAREFSIQFS GHAFTRGELL VFKFDDDKGK SYTLALTVTS ILGIDLSLAT NPQTVANLKP
IEIDAGQLLP NSVIVFDKAE DSLLNLTGKS KGKSAYRSII NPDWDFQKMG IGGLDKEFSG
IFRRAFASRV FPPEFIEQLG MKHVRGILLY GPPGTGKTLM ARQIGKMLNA REPKIINGPQ
ILDKYVGESE SNIRKLFADA EEEWKRCGAS SGLHIIIFDE IDAICKQRGS VAGSTAVHDT
VVNQLLAKMD GVDQLNNILV IGMTNRKDMI DEALLRPGRM EVQMEISLPD EAGRLQILKI
HTARMREYDK LDPNVDLIEL AKKTKNFSGA ELEGLVRAAQ SSAMNRLVKA GGKVQLDSDA
VEKLMINVDD FNYALENDVK PAFGHSNEEL EKYLIGGFIS WSTQVTQILE QGALLVKQVR
SPDTKGFTSV LLAGSPNSGK TCFAAMIAKA SDYPFIKVIS AEDMVGYTET AKCAALRKVF
DDAYRSPLSC VIVDGVERLL DFSPIGPRYS NLVLQALFLL VKKLPPQNRR LLVVATSSNR
SFLRELGLLS AFGTIIDVPA LTTVQHIMAV IEDTNALSRQ ECEEIRNELS RTNKEYFIGI
KKLLNVIDMA RECEPVDRVS VVVQSLMSET FSNS
//
