ID A0A1Q3E6A4_LENED Unreviewed; 1773 AA.
AC A0A1Q3E6A4;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 28-JAN-2026, entry version 35.
DE RecName: Full=DNA-directed RNA polymerase subunit {ECO:0000256|RuleBase:RU004279};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU004279};
GN ORFNames=LENED_004433 {ECO:0000313|EMBL:GAW02765.1};
OS Lentinula edodes (Shiitake mushroom) (Lentinus edodes).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC Agaricomycetidae; Agaricales; Marasmiineae; Omphalotaceae; Lentinula.
OX NCBI_TaxID=5353 {ECO:0000313|EMBL:GAW02765.1, ECO:0000313|Proteomes:UP000188533};
RN [1] {ECO:0000313|EMBL:GAW02765.1, ECO:0000313|Proteomes:UP000188533}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 111202 {ECO:0000313|EMBL:GAW02765.1,
RC ECO:0000313|Proteomes:UP000188533};
RG Lentinula edodes genome sequencing consortium;
RA Sakamoto Y., Nakade K., Sato S., Yoshida Y., Miyazaki K., Natsume S.,
RA Konno N.;
RL Submitted (AUG-2016) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:GAW02765.1, ECO:0000313|Proteomes:UP000188533}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 111202 {ECO:0000313|EMBL:GAW02765.1,
RC ECO:0000313|Proteomes:UP000188533};
RA Sakamoto Y., Nakade K., Sato S., Yoshida Y., Miyazaki K., Natsume S.,
RA Konno N.;
RT "A genome survey and senescence transcriptome analysis in Lentinula
RT edodes.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU004279}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) +
CC diphosphate; Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00048552,
CC ECO:0000256|RuleBase:RU004279};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta' chain family.
CC {ECO:0000256|ARBA:ARBA00006460, ECO:0000256|RuleBase:RU004279}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAW02765.1}.
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DR EMBL; BDGU01000112; GAW02765.1; -; Genomic_DNA.
DR STRING; 5353.A0A1Q3E6A4; -.
DR Proteomes; UP000188533; Unassembled WGS sequence.
DR GO; GO:0005665; C:RNA polymerase II, core complex; IEA:TreeGrafter.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006368; P:transcription elongation by RNA polymerase II; IEA:UniProtKB-ARBA.
DR GO; GO:0006367; P:transcription initiation at RNA polymerase II promoter; IEA:UniProtKB-ARBA.
DR CDD; cd02584; RNAP_II_Rpb1_C; 1.
DR CDD; cd02733; RNAP_II_RPB1_N; 1.
DR FunFam; 2.40.40.20:FF:000019; DNA-directed RNA polymerase II subunit RPB1; 1.
DR FunFam; 1.10.132.30:FF:000001; DNA-directed RNA polymerase subunit; 1.
DR FunFam; 1.10.150.390:FF:000001; DNA-directed RNA polymerase subunit; 1.
DR FunFam; 1.10.274.100:FF:000001; DNA-directed RNA polymerase subunit; 1.
DR FunFam; 3.30.1360.140:FF:000001; DNA-directed RNA polymerase subunit; 1.
DR FunFam; 3.30.1490.180:FF:000001; DNA-directed RNA polymerase subunit; 1.
DR FunFam; 4.10.860.120:FF:000003; DNA-directed RNA polymerase subunit; 1.
DR Gene3D; 1.10.132.30; -; 1.
DR Gene3D; 1.10.150.390; -; 1.
DR Gene3D; 2.40.40.20; -; 1.
DR Gene3D; 3.30.1360.140; -; 1.
DR Gene3D; 6.10.250.2940; -; 1.
DR Gene3D; 6.20.50.80; -; 1.
DR Gene3D; 3.30.1490.180; RNA polymerase ii; 1.
DR Gene3D; 4.10.860.120; RNA polymerase II, clamp domain; 2.
DR Gene3D; 1.10.274.100; RNA polymerase Rpb1, domain 3; 1.
DR InterPro; IPR045867; DNA-dir_RpoC_beta_prime.
DR InterPro; IPR000722; RNA_pol_asu.
DR InterPro; IPR000684; RNA_pol_II_repeat_euk.
DR InterPro; IPR006592; RNA_pol_N.
DR InterPro; IPR007080; RNA_pol_Rpb1_1.
DR InterPro; IPR007066; RNA_pol_Rpb1_3.
DR InterPro; IPR042102; RNA_pol_Rpb1_3_sf.
DR InterPro; IPR007083; RNA_pol_Rpb1_4.
DR InterPro; IPR007081; RNA_pol_Rpb1_5.
DR InterPro; IPR007075; RNA_pol_Rpb1_6.
DR InterPro; IPR007073; RNA_pol_Rpb1_7.
DR InterPro; IPR038593; RNA_pol_Rpb1_7_sf.
DR InterPro; IPR044893; RNA_pol_Rpb1_clamp_domain.
DR InterPro; IPR038120; Rpb1_funnel_sf.
DR NCBIfam; NF006336; PRK08566.1; 1.
DR PANTHER; PTHR19376; DNA-DIRECTED RNA POLYMERASE; 1.
DR PANTHER; PTHR19376:SF37; DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB1; 1.
DR Pfam; PF04997; RNA_pol_Rpb1_1; 1.
DR Pfam; PF00623; RNA_pol_Rpb1_2; 1.
DR Pfam; PF04983; RNA_pol_Rpb1_3; 1.
DR Pfam; PF05000; RNA_pol_Rpb1_4; 1.
DR Pfam; PF04998; RNA_pol_Rpb1_5; 1.
DR Pfam; PF04992; RNA_pol_Rpb1_6; 1.
DR Pfam; PF04990; RNA_pol_Rpb1_7; 1.
DR Pfam; PF05001; RNA_pol_Rpb1_R; 3.
DR SMART; SM00663; RPOLA_N; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
DR PROSITE; PS00115; RNA_POL_II_REPEAT; 2.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU004279}; Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU004279}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000188533};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU004279};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU004279};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT DOMAIN 243..546
FT /note="RNA polymerase N-terminal"
FT /evidence="ECO:0000259|SMART:SM00663"
FT REGION 152..177
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1545..1568
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1598..1662
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 152..165
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1545..1555
FT /note="Gly residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1556..1568
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1601..1618
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1619..1662
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1773 AA; 198084 MW; CFFB9C5C54A8C0D2 CRC64;
MLGHQFAFSA APVRRVKEVQ FGILSPEEIK AYSVAKIEHP EVMDEGTHKP KMGGLMDPRL
GTIDRNFKCQ TCGEGMAECP GHFGHIELAR PVFHPGFIIK VKKILECICV NCGKLKADIA
DPNFADKIRH VRDPKKRMAV VWSHCKSKMV CEPDDPKEEG AEPEPEDTKK GHGGCGHIQP
LVRKEGLKLF LQYKKPKDDD EEIKSMQPDK RLMTPQEVYT TFKKMSDSDL HLIGLSEEFA
RPEWMILTVM PVPPPPVRPS IAVDGGAMRS EDDLTYKLGD IIKASANVRR CEQEGAPAHV
ITEFEQLLQF HVATYMDNDI AGIPQALQKS GRPVKAIRAR LKGKEGRLRG NLMGKRVDFS
ARTVITGDPN LELDEVGVPR SIAMNLTFPE RVTPYNITYL QELVRNGPTT YPGARYVVRD
TGERIDLRYN KRADAFLQYG WVVERHLKDG DFVLFNRQPS LHKMSMMSHR VRLMPYSTFR
LNLSVTPPYN ADFDGDEMNM HVPQSEETRA ELSQIAWVAR QIISPQANKP VMGIVQDTLC
GIRKFTLRDS FLDWNQVQNI LLWVPEWDGS VPIPAILKPK PLWTGKQILS LVIPRGINIH
RSPDPKSSNP VFDDGMLIEN GEIIFGIVEK KTVGASQGGL VHVVFREKGP EVTRQLFTGL
QTVVNYWLFH NGFSIGIGDT IADKNTMSYI TETIASRKAN VSKIIEDATH DRLKPAPGMT
IRESFESLVE RELNLARDTS GQHAQKSLKE DNNVKQMVVA GSKGSFINIS QMSVCVGQQS
VEGRRIPFGF RHRTLPHFTK DDFSPESRGF VENSYLRGLT PQEFFFHAMA GREGLIDTAV
KTAETGYIQR RLVKALEDVM VCYDGTVRNS LGDLIQFVYG EDGMDGAFIE KQNIDTFSLN
DREFEHNYRV DVTDPAGGFL PGVLQVGIDD SSLELQNKLD EEYDQLLSDR RLLREFIFPQ
HATSFNQYLP VNLSRIVQNA AQIFHIDRRK PSDLDPAFII DSVKTLCDRL VVVRGDGPLS
HEAQANATLL FRMHLRATFG CRRVLERFHL NKEAFEWVLG EVEAKFNQSV ANPGEMCGTL
AAQSIGEPAT QMTLNTFHYA GVSSKNVTLG VPRLKEIINV ATNIKTPSLS VYLEPALRFD
PNLAKNVQQE LAYTSLRTVT AAVEIWYDPD PSSTIIEEDE VFVESFFAIP DEEVESKLHL
QSPWLLRLEL DRAKMLDRKL TMAYVASRIA ESFKTDLFVI WSEDNAEKLV IRCRVLGGAD
KDEDGTETLE EDIFLRQLEN TMLNSVSLRG VQGINRVFLT RHDLVETKDD GSIVAEKDKQ
WVLETDGVNL KTVMCIDGVD FTRTYSNSCV EVFNVLGIEA ARAAILKELR GVIEFDGSYV
NYRHLALLCD LMTHRGSLMA ITRHGINRAD TGALMRCSFE ETVEILMEAA AVGEKDDCHG
VAENVMFGQM APMGTGAFDV ALDIDMLKDA IVDHRLPVQS MLAAQVDGGM TPGQVAMTPY
DTNSPMWDNH LFKGSDQAAF SPLAVNSNEE SGNFNYLPWG QSPRGAGGMS PGGPGYSPSS
PNAYSPNSPA FAPTSPFGGG ATSPMVYGGT SPYYDPGRGV TSPTYSPTSP AFNPTSPAFS
PTSPRYSPTS PSFSPTSPRY SPQSPSFSPT SPRYSPTSPS FSPASPRLFT LCVFIRFADR
SSSTRNFIFQ LPLRNYLLHP LDIHPLLRWP RQLRPDILLL LPRTLLRRPL TPPHLPHTVL
HLHYAHPRAL LRTGPLTAVT HTSHLLLGID QSW
//
