ID A0A1S2YN84_CICAR Unreviewed; 588 AA.
AC A0A1S2YN84;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 18-JUN-2025, entry version 39.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN Name=LOC101493018 {ECO:0000313|RefSeq:XP_004507320.1};
OS Cicer arietinum (Chickpea) (Garbanzo).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Cicereae; Cicer.
OX NCBI_TaxID=3827 {ECO:0000313|Proteomes:UP000087171, ECO:0000313|RefSeq:XP_004507320.1};
RN [1] {ECO:0000313|Proteomes:UP000087171}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. CDC Frontier {ECO:0000313|Proteomes:UP000087171};
RX PubMed=23354103;
RA Varshney R.K., Song C., Saxena R.K., Azam S., Yu S., Sharpe A.G.,
RA Cannon S., Baek J., Rosen B.D., Tar'an B., Millan T., Zhang X.,
RA Ramsay L.D., Iwata A., Wang Y., Nelson W., Farmer A.D., Gaur P.M.,
RA Soderlund C., Penmetsa R.V., Xu C., Bharti A.K., He W., Winter P., Zhao S.,
RA Hane J.K., Carrasquilla-Garcia N., Condie J.A., Upadhyaya H.D., Luo M.C.,
RA Thudi M., Gowda C.L., Singh N.P., Lichtenzveig J., Gali K.K., Rubio J.,
RA Nadarajan N., Dolezel J., Bansal K.C., Xu X., Edwards D., Zhang G.,
RA Kahl G., Gil J., Singh K.B., Datta S.K., Jackson S.A., Wang J., Cook D.R.;
RT "Draft genome sequence of chickpea (Cicer arietinum) provides a resource
RT for trait improvement.";
RL Nat. Biotechnol. 31:240-246(2013).
RN [2] {ECO:0000313|RefSeq:XP_004507320.1}
RP IDENTIFICATION.
RC TISSUE=Etiolated seedlings {ECO:0000313|RefSeq:XP_004507320.1};
RG RefSeq;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
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DR RefSeq; XP_004507320.1; XM_004507263.2.
DR AlphaFoldDB; A0A1S2YN84; -.
DR STRING; 3827.A0A1S2YN84; -.
DR PaxDb; 3827-XP_004507320.1; -.
DR GeneID; 101493018; -.
DR KEGG; cam:101493018; -.
DR eggNOG; KOG1815; Eukaryota.
DR OrthoDB; 10009520at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000087171; Chromosome Ca6.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd20346; BRcat_RBR_ANKIB1; 1.
DR CDD; cd22583; Rcat_RBR_ARI7-like; 1.
DR FunFam; 1.20.120.1750:FF:000005; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000019; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR045840; Ariadne.
DR InterPro; IPR048962; ARIH1-like_UBL.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001876; Znf_RanBP2.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF19422; Ariadne; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22191; IBR_1; 1.
DR Pfam; PF21235; UBA_ARI1; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS01358; ZF_RANBP2_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000087171};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 134..347
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 138..182
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 1..34
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 529..556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 588 AA; 66946 MW; 8EE69CFB354CB971 CRC64;
MDSEDDMHDA NDVESLEDDD DFYSGDTEDA SMDYYSDYDD DAGDYFDDAD KIESRRPEQN
FTILKESDIR VRQEDDITRV AAVLSLSRVF ASILLRHYNW SVSKVHDAWF ADEERVRKAV
GLLEKPVVQS PNARELTCGI CFENHPRSVI EMASCGHPYC FTCWEGYIST SINDGPGCLM
LRCPEPTCGS AIGQDMINLL ASEEDKQKYA RYLLRSYIED NKKSKWCPAP GCEYAVTFDA
GGGNYDVSCL CSYSFCWNCT EEAHRPVDCG TVVKWILKNS AESENMNWIL ANSKPCPKCK
RPIEKNQGCM HITCTPPCKF EFCWLCLGAW SDHGERTGGF YACNRYEAAK QEGVYDDTER
RREMAKNSLE RYTHYYERWA SNQSSRQKAL ADLHQMQTVH IEKLSDTQCQ PESQLKFITE
AWLQIVECRR VLKWTYAYGY YLPEHERAKK QFFEYLQGEA ESGLERLHQC AEKELQQFLS
ADGPSKEFND FRTKLAGLTS VTKNYFENLV RALENGLSDV DGNGAAISKA TSSKNAAGSS
KGRTGRGKGA FRTTMTSRTS DDSHWCCDHC TSTNVRSATV CQICNQRR
//
