ID A0A1S3EBG2_CICAR Unreviewed; 534 AA.
AC A0A1S3EBG2;
DT 12-APR-2017, integrated into UniProtKB/TrEMBL.
DT 12-APR-2017, sequence version 1.
DT 18-JUN-2025, entry version 41.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN Name=LOC101501302 {ECO:0000313|RefSeq:XP_012572758.1};
OS Cicer arietinum (Chickpea) (Garbanzo).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Cicereae; Cicer.
OX NCBI_TaxID=3827 {ECO:0000313|Proteomes:UP000087171, ECO:0000313|RefSeq:XP_012572758.1};
RN [1] {ECO:0000313|Proteomes:UP000087171}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. CDC Frontier {ECO:0000313|Proteomes:UP000087171};
RX PubMed=23354103;
RA Varshney R.K., Song C., Saxena R.K., Azam S., Yu S., Sharpe A.G.,
RA Cannon S., Baek J., Rosen B.D., Tar'an B., Millan T., Zhang X.,
RA Ramsay L.D., Iwata A., Wang Y., Nelson W., Farmer A.D., Gaur P.M.,
RA Soderlund C., Penmetsa R.V., Xu C., Bharti A.K., He W., Winter P., Zhao S.,
RA Hane J.K., Carrasquilla-Garcia N., Condie J.A., Upadhyaya H.D., Luo M.C.,
RA Thudi M., Gowda C.L., Singh N.P., Lichtenzveig J., Gali K.K., Rubio J.,
RA Nadarajan N., Dolezel J., Bansal K.C., Xu X., Edwards D., Zhang G.,
RA Kahl G., Gil J., Singh K.B., Datta S.K., Jackson S.A., Wang J., Cook D.R.;
RT "Draft genome sequence of chickpea (Cicer arietinum) provides a resource
RT for trait improvement.";
RL Nat. Biotechnol. 31:240-246(2013).
RN [2] {ECO:0000313|RefSeq:XP_012572758.1}
RP IDENTIFICATION.
RC TISSUE=Etiolated seedlings {ECO:0000313|RefSeq:XP_012572758.1};
RG RefSeq;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- FUNCTION: Might act as an E3 ubiquitin-protein ligase, or as part of E3
CC complex, which accepts ubiquitin from specific E2 ubiquitin-conjugating
CC enzymes and then transfers it to substrates.
CC {ECO:0000256|ARBA:ARBA00003976}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the RBR family. Ariadne subfamily.
CC {ECO:0000256|ARBA:ARBA00005884}.
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DR RefSeq; XP_012572758.1; XM_012717304.2.
DR STRING; 3827.A0A1S3EBG2; -.
DR GeneID; 101501302; -.
DR KEGG; cam:101501302; -.
DR OrthoDB; 9977870at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000087171; Chromosome Ca6.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0004523; F:RNA-DNA hybrid ribonuclease activity; IEA:InterPro.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd22582; BRcat_RBR_unk; 1.
DR CDD; cd22584; Rcat_RBR_unk; 1.
DR FunFam; 1.20.120.1750:FF:000019; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000230; RBR-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.420.10:FF:000076; RBR-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR002156; RNaseH_domain.
DR InterPro; IPR036397; RNaseH_sf.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR017907; Znf_RING_CS.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 2.
DR Pfam; PF13456; RVT_3; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
DR PROSITE; PS00518; ZF_RING_1; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000087171};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 290..507
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT DOMAIN 294..341
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 124..149
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 514..534
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 133..143
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 534 AA; 60371 MW; D0989EBC9D902A16 CRC64;
MDSPEDLQFL LSAHRHALTE AEAMESDLDF AFHLQVQEAL AASLVNHPSS SIAVTFEPPT
LNDDVLNATS IQLKELEKME TEMKDREYSE RVMREARDDL NRRIHDQKMA SEILNMPEDE
WEQWGGNFEK PFGEGSSSSG SKSGNDEGXF FEGTGENVSL AGIGVAICDV ADNLILEVSK
PVVGSGNSKV AVELKALIEA FDAAIALDLK RVICFGDHYP LFQFLSGKWP AKQRKVAMLV
DQVNLLQRKF AYCSPRLVAR HDQKFAFKLA KDAIVSQSKR PAESGSVKSM NETCVICLED
TDVSQFFSVD GCQHRYCFSC MKQHVEVKLL HGMVPKCPHE KCNNELLVDS CRKFLTSKLV
ETMQQRKLEA SIPLTEKVYC PYPRCSALMS KSEVLDYSKS VIASVESQPM KCIKCHGLFC
FSCKVPWHSD MTCSTYKRLN PNPPAEDMKL KFLARTSLWR QCVKCNHMIE LAEGCYHMTC
RCGYEFCYNC GAEWKDKKAT CSCPLWEEDN ILLQDPDEEP EADEVNSDYS DDWY
//
