ID A0A1U8JY01_GOSHI Unreviewed; 444 AA.
AC A0A1U8JY01;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 10-MAY-2017, sequence version 1.
DT 28-JAN-2026, entry version 37.
DE RecName: Full=U-box domain-containing protein {ECO:0000256|RuleBase:RU369093, ECO:0000259|PROSITE:PS51698};
DE EC=2.3.2.27 {ECO:0000256|RuleBase:RU369093};
DE AltName: Full=RING-type E3 ubiquitin transferase PUB {ECO:0000256|RuleBase:RU369093};
GN Name=LOC107909964 {ECO:0000313|RefSeq:XP_016693169.1};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000818029, ECO:0000313|RefSeq:XP_016693169.1};
RN [1] {ECO:0000313|Proteomes:UP000818029}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000818029};
RX PubMed=32313247; DOI=10.1038/s41588-020-0614-5;
RA Chen Z.J., Sreedasyam A., Ando A., Song Q., De Santiago L.M.,
RA Hulse-Kemp A.M., Ding M., Ye W., Kirkbride R.C., Jenkins J., Plott C.,
RA Lovell J., Lin Y.M., Vaughn R., Liu B., Simpson S., Scheffler B.E., Wen L.,
RA Saski C.A., Grover C.E., Hu G., Conover J.L., Carlson J.W., Shu S.,
RA Boston L.B., Williams M., Peterson D.G., McGee K., Jones D.C., Wendel J.F.,
RA Stelly D.M., Grimwood J., Schmutz J.;
RT "Genomic diversifications of five Gossypium allopolyploid species and their
RT impact on cotton improvement.";
RL Nat. Genet. 52:525-533(2020).
RN [2] {ECO:0000313|RefSeq:XP_016693169.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (AUG-2025) to UniProtKB.
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|RuleBase:RU369093}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900,
CC ECO:0000256|RuleBase:RU369093};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906, ECO:0000256|RuleBase:RU369093}.
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DR RefSeq; XP_016693169.1; XM_016837680.2.
DR AlphaFoldDB; A0A1U8JY01; -.
DR SMR; A0A1U8JY01; -.
DR STRING; 3635.A0A1U8JY01; -.
DR PaxDb; 3635-A0A1U8JY01; -.
DR GeneID; 107909964; -.
DR KEGG; ghi:107909964; -.
DR OrthoDB; 10064100at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000818029; Chromosome D02.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniRule.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR FunFam; 3.30.40.10:FF:000442; RING-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR058678; ARM_PUB.
DR InterPro; IPR045185; PUB22/23/24-like.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR22849:SF139; U-BOX DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR22849; WDSAM1 PROTEIN; 1.
DR Pfam; PF25598; ARM_PUB; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000818029};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU369093};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|RuleBase:RU369093}.
FT DOMAIN 31..105
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
FT REGION 1..26
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..12
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 444 AA; 49894 MW; 7F2569D4A92E5C55 CRC64;
MMISSWRRRR AARREGKLQQ QRNDNGEIEL TVPRDFRCPI SLDLMKDPVT LSTGITYDRE
NIEKWIEAGN FTCPLTNQVL RSLEPIPNHI IRKKIQDWCV ENRSYGIERI PTPRVPVTSM
EVSDILSKIN VACMKQNGKG CRDLVLKIKS LAKESERNKR CFVSNGAGCA LSEAFQAFSR
ESFDENVAVL EEILSALAIM FPLDVEAKGF LGSASAMRCL IWFLSSGDLS RRRNAVLGLR
ELVSIDERKV NELSDMEGAI EALFKLIKDP ICPTSTKASL LVIYKIITSS PTKEKQVKKL
VNLGIVSLLL ETLVDSERGI CEKALGVLDG ICNSEEGRQM ACNNALSMPV LVKKILRVSN
LTTDFSVSIL WKLCKKEKTE DDASVILEAL QVGAFLKLLL LLQVGCVEET KDKASELLKI
LNLHRNKMEC VDPMDNFKDL KRPF
//
