ID A0A1U8NWT5_GOSHI Unreviewed; 893 AA.
AC A0A1U8NWT5;
DT 10-MAY-2017, integrated into UniProtKB/TrEMBL.
DT 08-OCT-2025, sequence version 2.
DT 28-JAN-2026, entry version 32.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN Name=LOC107952768 {ECO:0000313|RefSeq:XP_016743427.2};
OS Gossypium hirsutum (Upland cotton) (Gossypium mexicanum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Malvales; Malvaceae; Malvoideae; Gossypium.
OX NCBI_TaxID=3635 {ECO:0000313|Proteomes:UP000818029, ECO:0000313|RefSeq:XP_016743427.2};
RN [1] {ECO:0000313|Proteomes:UP000818029}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. TM-1 {ECO:0000313|Proteomes:UP000818029};
RX PubMed=32313247; DOI=10.1038/s41588-020-0614-5;
RA Chen Z.J., Sreedasyam A., Ando A., Song Q., De Santiago L.M.,
RA Hulse-Kemp A.M., Ding M., Ye W., Kirkbride R.C., Jenkins J., Plott C.,
RA Lovell J., Lin Y.M., Vaughn R., Liu B., Simpson S., Scheffler B.E., Wen L.,
RA Saski C.A., Grover C.E., Hu G., Conover J.L., Carlson J.W., Shu S.,
RA Boston L.B., Williams M., Peterson D.G., McGee K., Jones D.C., Wendel J.F.,
RA Stelly D.M., Grimwood J., Schmutz J.;
RT "Genomic diversifications of five Gossypium allopolyploid species and their
RT impact on cotton improvement.";
RL Nat. Genet. 52:525-533(2020).
RN [2] {ECO:0000313|RefSeq:XP_016743427.2}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (AUG-2025) to UniProtKB.
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|ARBA:ARBA00003861}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR RefSeq; XP_016743427.2; XM_016887938.2.
DR AlphaFoldDB; A0A1U8NWT5; -.
DR GeneID; 107952768; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000818029; Chromosome D07.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd16655; RING-Ubox_WDSUB1-like; 1.
DR CDD; cd14066; STKc_IRAK; 1.
DR CDD; cd01989; USP_STK_Ubox_N; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR Gene3D; 3.30.200.20; Phosphorylase Kinase, domain 1; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR051348; U-box_ubiquitin_ligases.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR45647; OS02G0152300 PROTEIN; 1.
DR PANTHER; PTHR45647:SF100; U-BOX DOMAIN-CONTAINING PROTEIN 33; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00220; S_TKc; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Coiled coil {ECO:0000256|SAM:Coils};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000818029};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT DOMAIN 541..804
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT DOMAIN 822..893
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
FT REGION 219..320
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 371..513
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 219..257
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 286..302
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..320
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 568
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 893 AA; 99928 MW; F2588869DC896116 CRC64;
MALVSVVPAI TEGMNSMRFC DVRAPGILSS GREIVEETVA RIIEEKIYVA VGKNVDKYKS
VLFWALQHSG GKKICIIHVH QPAKMIPVSE MGTKFPASKL EEQEVKAYWE IERKNMEKML
NDYLLLCLQR GVQAEKLYIE RDSIEQGILE MISENRIRML VMGGAADKHY SKKVVDLKSK
KAIFVRENAP NTCHTIWFLC KGLLIYTRKL SSDVTDTKVA SSSLPASPNL ESSENHFRSQ
SVILRQTSRV KPSTSAPDSL RRVRSENVYG HVGSTLGFPS PDGNEGLSTP WNRSDVEGSS
NEWDGLSRSP QNSVLSSSSS NGMADAALVP YMGTEVNGNG LQSSLIPHAE GNFNLSSLPN
IQDVTTDNTL YDQLQKVMAE ATNSRREAFE EAMKRSEAEK DALVSIRRVK ASEILYAQEL
KQRKEIEEAL AKEKDELGKM KNQRDEVRIE LQAALGQKSS LENQIAESEK EVKELQEKIF
SAVELLQNYK KERDELQMER DNALKEAEEL RKSRAEPSGA HMHQFFTEFS FTEIEEATLN
FDPSLKIGEG GYGSIYKGNL RHTTVAIKRL HSNSLQGPSE FQQEVDVLSK MRHPNLVTLI
GACPDAWTLI YEYLPNGSLE DRLSCRGNSP PLSWQTRIRL ATELCSVLIF LHSSKPHGIV
HGDLKPANIL LDANFVTKLS DFGICRLLSD NTTICCRTDP KGTFAYMDPE FLSTGELTQK
ADVYSFGVIL LRLLTGRQAL GIIKEVQYAL DNGSLKNLLD PLAGDWPFVQ AEQLANLALR
CCEMNRRCRP DLSTDVWRVL EPMRASCGGS SSFQLGSEEQ CHPPSYFICP IFQEVMQDPH
VAADGFTYEA EALRGWLDSG HDTSPMTNIK LAHSNLVPNL ALRSAIQEWL QQH
//
