ID A0A226GRQ8_9FLAO Unreviewed; 791 AA.
AC A0A226GRQ8;
DT 25-OCT-2017, integrated into UniProtKB/TrEMBL.
DT 25-OCT-2017, sequence version 1.
DT 18-JUN-2025, entry version 27.
DE RecName: Full=peptidoglycan glycosyltransferase {ECO:0000256|ARBA:ARBA00044770};
DE EC=2.4.99.28 {ECO:0000256|ARBA:ARBA00044770};
GN ORFNames=B0A66_21050 {ECO:0000313|EMBL:OXA84374.1};
OS Flavobacterium hercynium.
OC Bacteria; Pseudomonadati; Bacteroidota; Flavobacteriia; Flavobacteriales;
OC Flavobacteriaceae; Flavobacterium.
OX NCBI_TaxID=387094 {ECO:0000313|EMBL:OXA84374.1, ECO:0000313|Proteomes:UP000198345};
RN [1] {ECO:0000313|EMBL:OXA84374.1, ECO:0000313|Proteomes:UP000198345}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DSM 18292 {ECO:0000313|EMBL:OXA84374.1,
RC ECO:0000313|Proteomes:UP000198345};
RA Stine C., Li C., Tadesse D.;
RT "Whole genomes of Flavobacteriaceae.";
RL Submitted (NOV-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-
CC Ala)](n)-di-trans,octa-cis-undecaprenyl diphosphate + beta-D-GlcNAc-
CC (1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-di-trans,octa-
CC cis-undecaprenyl diphosphate = [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-
CC D-Glu-L-Lys-D-Ala-D-Ala)](n+1)-di-trans,octa-cis-undecaprenyl
CC diphosphate + di-trans,octa-cis-undecaprenyl diphosphate + H(+);
CC Xref=Rhea:RHEA:23708, Rhea:RHEA-COMP:9602, Rhea:RHEA-COMP:9603,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58405, ChEBI:CHEBI:60033,
CC ChEBI:CHEBI:78435; EC=2.4.99.28;
CC Evidence={ECO:0000256|ARBA:ARBA00049902};
CC -!- PATHWAY: Cell wall biogenesis; peptidoglycan biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004752}.
CC -!- SIMILARITY: In the C-terminal section; belongs to the transpeptidase
CC family. {ECO:0000256|ARBA:ARBA00007090}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the
CC glycosyltransferase 51 family. {ECO:0000256|ARBA:ARBA00007739}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OXA84374.1}.
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DR EMBL; MUGW01000062; OXA84374.1; -; Genomic_DNA.
DR RefSeq; WP_089051819.1; NZ_FXTV01000001.1.
DR AlphaFoldDB; A0A226GRQ8; -.
DR OrthoDB; 9766909at2; -.
DR Proteomes; UP000198345; Unassembled WGS sequence.
DR GO; GO:0030288; C:outer membrane-bounded periplasmic space; IEA:TreeGrafter.
DR GO; GO:0004180; F:carboxypeptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0008658; F:penicillin binding; IEA:InterPro.
DR GO; GO:0008955; F:peptidoglycan glycosyltransferase activity; IEA:InterPro.
DR GO; GO:0009252; P:peptidoglycan biosynthetic process; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.10.3810.10; Biosynthetic peptidoglycan transglycosylase-like; 1.
DR Gene3D; 3.40.710.10; DD-peptidase/beta-lactamase superfamily; 1.
DR InterPro; IPR012338; Beta-lactam/transpept-like.
DR InterPro; IPR001264; Glyco_trans_51.
DR InterPro; IPR050396; Glycosyltr_51/Transpeptidase.
DR InterPro; IPR023346; Lysozyme-like_dom_sf.
DR InterPro; IPR011815; PBP_1c.
DR InterPro; IPR009647; PBP_C.
DR InterPro; IPR036950; PBP_transglycosylase.
DR InterPro; IPR001460; PCN-bd_Tpept.
DR NCBIfam; TIGR02073; PBP_1c; 1.
DR PANTHER; PTHR32282; BINDING PROTEIN TRANSPEPTIDASE, PUTATIVE-RELATED; 1.
DR PANTHER; PTHR32282:SF15; PENICILLIN-BINDING PROTEIN 1C; 1.
DR Pfam; PF06832; BiPBP_C; 1.
DR Pfam; PF00912; Transgly; 1.
DR Pfam; PF00905; Transpeptidase; 1.
DR SUPFAM; SSF56601; beta-lactamase/transpeptidase-like; 1.
DR SUPFAM; SSF53955; Lysozyme-like; 1.
PE 3: Inferred from homology;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022670};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000198345};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 71..230
FT /note="Glycosyl transferase family 51"
FT /evidence="ECO:0000259|Pfam:PF00912"
FT DOMAIN 309..569
FT /note="Penicillin-binding protein transpeptidase"
FT /evidence="ECO:0000259|Pfam:PF00905"
FT DOMAIN 697..785
FT /note="Penicillin-binding C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06832"
SQ SEQUENCE 791 AA; 90081 MW; BA4AA7E23CBB82EB CRC64;
MKNKLITFVQ RIINWVKENK KKSVIAFVLL LLYYFAIPRT LFQEPYSTVI ESKEGELLGA
KIARDGQWRF PAQDSVPDKF KKCIVYFEDE YFYKHPGFNP VAMINAIKQN RKAGKVVRGG
STLTQQVIRL SRKGKGRTYF EKIIEIILAT RLELGYSKNE IVELYAAHAP FGGNVVGLEM
ASWRYFGVQS NQLSWAENAT LAVLPNAPSL IYPGKNQIKL LNKRNRLLLK LHQEDVIDKQ
TYELAIEEPL PQKPYDLPQI APHLLQRIAK NEEGTRVKTT VDFALQNRVN QIAKYYYNQY
KQNEVHNLAI LVIDVSNRNV MSYVGNAPAD KYHQKDVDII SAPRSTGSIL KPLLYASMLD
DGELLPNTLV ADVPTQISGY TPENYNLTFD GAVPAHRALS RSLNIPAVLM LQDFGVNKFY
EELQKFKLRD INKTPDHYGL SLILGGAESN LWDLCRTYAG LSSTLNYFTS NQGKYRTKEF
AEINYKNDAE VDFGEESTQK NILGSGSIWL TYNAMEEVNR PEGDEAWKFY DSSLKIAWKT
GTSFGNRDAW AIGTNSKYVV GVWVGNATGE GRPTLTGVSS AAPILFDVFN LLPRQKWFQT
PYNDLTAVEV CSLSGYLAKE ECPKIKQWVS VKGKATVVCP YHKTVHLDKT EQFQVNSSCE
SIDNIVTKSW FVLPPVMAWY YKSQHIAYQP LPPFKEDCQG TQSATMDFIY PKPNSKIYLT
KNFNSEVQPV ILKVAYSERD KELFWYLDDV YKATTKTFHE LQITPTAGTH HITVVDAYGN
EIRRKIEIIR E
//
