ID A0A251UK88_HELAN Unreviewed; 1162 AA.
AC A0A251UK88;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 18-JUN-2025, entry version 32.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000256|RuleBase:RU363031};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU363031};
GN ORFNames=HannXRQ_Chr06g0183351 {ECO:0000313|EMBL:OTG23514.1},
GN HanXRQr2_Chr15g0693661 {ECO:0000313|EMBL:KAF5764572.1};
OS Helianthus annuus (Common sunflower).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC Heliantheae alliance; Heliantheae; Helianthus.
OX NCBI_TaxID=4232 {ECO:0000313|EMBL:OTG23514.1, ECO:0000313|Proteomes:UP000215914};
RN [1] {ECO:0000313|EMBL:KAF5764572.1, ECO:0000313|Proteomes:UP000215914}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. SF193 {ECO:0000313|Proteomes:UP000215914};
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF5764572.1};
RX PubMed=28538728; DOI=10.1038/nature22380;
RA Badouin H., Gouzy J., Grassa C.J., Murat F., Staton S.E., Cottret L.,
RA Lelandais-Briere C., Owens G.L., Carrere S., Mayjonade B., Legrand L.,
RA Gill N., Kane N.C., Bowers J.E., Hubner S., Bellec A., Berard A.,
RA Berges H., Blanchet N., Boniface M.C., Brunel D., Catrice O., Chaidir N.,
RA Claudel C., Donnadieu C., Faraut T., Fievet G., Helmstetter N., King M.,
RA Knapp S.J., Lai Z., Le Paslier M.C., Lippi Y., Lorenzon L., Mandel J.R.,
RA Marage G., Marchand G., Marquand E., Bret-Mestries E., Morien E.,
RA Nambeesan S., Nguyen T., Pegot-Espagnet P., Pouilly N., Raftis F.,
RA Sallet E., Schiex T., Thomas J., Vandecasteele C., Vares D., Vear F.,
RA Vautrin S., Crespi M., Mangin B., Burke J.M., Salse J., Munos S.,
RA Vincourt P., Rieseberg L.H., Langlade N.B.;
RT "The sunflower genome provides insights into oil metabolism, flowering and
RT Asterid evolution.";
RL Nature 546:148-152(2017).
RN [2] {ECO:0000313|EMBL:OTG23514.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Leaves {ECO:0000313|EMBL:OTG23514.1};
RA Langlade N., Munos S.;
RT "Sunflower complete genome.";
RL Submitted (FEB-2017) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000313|EMBL:KAF5764572.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaves {ECO:0000313|EMBL:KAF5764572.1};
RA Gouzy J., Langlade N., Munos S.;
RT "Helianthus annuus Genome sequencing and assembly Release 2.";
RL Submitted (JUN-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU363031}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) +
CC diphosphate; Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00048552,
CC ECO:0000256|RuleBase:RU363031};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000256|ARBA:ARBA00006835, ECO:0000256|RuleBase:RU000434}.
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DR EMBL; MNCJ02000330; KAF5764572.1; -; Genomic_DNA.
DR EMBL; CM007895; OTG23514.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A251UK88; -.
DR FunCoup; A0A251UK88; 4704.
DR STRING; 4232.A0A251UK88; -.
DR EnsemblPlants; mRNA:HanXRQr2_Chr15g0693661; mRNA:HanXRQr2_Chr15g0693661; HanXRQr2_Chr15g0693661.
DR Gramene; mRNA:HanXRQr2_Chr15g0693661; mRNA:HanXRQr2_Chr15g0693661; HanXRQr2_Chr15g0693661.
DR InParanoid; A0A251UK88; -.
DR OMA; IVRANHE; -.
DR OrthoDB; 10248617at2759; -.
DR Proteomes; UP000215914; Chromosome 6.
DR GO; GO:0005739; C:mitochondrion; IEA:GOC.
DR GO; GO:0009536; C:plastid; IEA:GOC.
DR GO; GO:0005666; C:RNA polymerase III complex; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR FunFam; 2.40.270.10:FF:000006; DNA-directed RNA polymerase subunit beta; 1.
DR FunFam; 2.40.270.10:FF:000011; DNA-directed RNA polymerase subunit beta; 1.
DR FunFam; 3.90.1070.20:FF:000002; DNA-directed RNA polymerase subunit beta; 1.
DR FunFam; 3.90.1100.10:FF:000021; DNA-directed RNA polymerase subunit beta; 1.
DR FunFam; 3.90.1800.10:FF:000002; DNA-directed RNA polymerase subunit beta; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1070.20; -; 1.
DR Gene3D; 3.90.1100.10; -; 1.
DR Gene3D; 2.40.270.10; DNA-directed RNA polymerase, subunit 2, domain 6; 1.
DR Gene3D; 3.90.1800.10; RNA polymerase alpha subunit dimerisation domain; 1.
DR Gene3D; 3.90.1110.10; RNA polymerase Rpb2, domain 2; 1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007646; RNA_pol_Rpb2_4.
DR InterPro; IPR007647; RNA_pol_Rpb2_5.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR PANTHER; PTHR20856; DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF04566; RNA_pol_Rpb2_4; 1.
DR Pfam; PF04567; RNA_pol_Rpb2_5; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU363031};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU363031}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000215914};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU363031};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363031};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 41..426
FT /note="RNA polymerase beta subunit protrusion"
FT /evidence="ECO:0000259|Pfam:PF04563"
FT DOMAIN 204..387
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04561"
FT DOMAIN 463..527
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04565"
FT DOMAIN 563..623
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04566"
FT DOMAIN 645..677
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04567"
FT DOMAIN 692..1066
FT /note="DNA-directed RNA polymerase subunit 2 hybrid-
FT binding"
FT /evidence="ECO:0000259|Pfam:PF00562"
FT DOMAIN 1068..1158
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04560"
SQ SEQUENCE 1162 AA; 130669 MW; E7060B5DB57504E1 CRC64;
MEIEDSTMHN GEVDKQFLAA PVKSPVDKFQ LIPEVWKARG LHLESFNYFV TTDIKKIVRA
NSLVTSEYNP EIYLRYKDVR IGRPSEIIDG VTEPLTPYKC RQSDKTYAAP IFVDIEYRNA
DSSYMENRKA GSSFIENRKA GFSLNKSNVI IGRMPIMLKS QSCVLHGKDE DELAKFGECP
LDPGGYFVVK GNEKVILIQE QLSKNRIIID KDNKGCIQAS VMSSCNITKK ISKTIIKMEK
GKIYLVLNSF STKVPIMLVL KAMGMQSDQE VVQMLGRDPQ YANLLLPSIE ESDQTLQKVK
NDGASNGVSY QQQALDYLQQ KRKYKSAKDD GALGVLRDTF LLNVPVRENN FHLKCVYVVV
MLRRMMDAIL NEDAMDDKDY VGNKRLELSG QLISILFEDL FKSLNENIRK QVDGDLKNGK
RVDISMILAG GCKQLITTGL ERSLSTGNWD VRRFKMHRKG ITQIVSRLSF INAFGSMTKI
QPQFEKSRKV SGPRALQPSQ WGMLCPCDTP EGEACGLVKN LALMTHVTTD EETGPIISLC
YCLGVDLSLL SGEELHSPRS FLIMLNGLIL GKHTKPQWFA DSMRKLRRAG KLSDFVSIYV
NEKHRCVYIA SDGGRVCRPL VIADNGVSRI KHQHMKELKD GARNFNGFLR EGLIEYLDVN
EENNSLIALY EGETTRETTH IEIEPFTILS ACAGLIPYPH HNQSPRNTYQ CAMGKQAMGN
IAYNQLSRLD SLIYLLVYPQ RPLLTTRTIE LIGYDKLGAG QNATVAVMSY SGYDIEDAIV
MNKASLDRGF GRCIVLKRML EQYKSYKNTS VRERICAPRR SQRDSDKMQI LDNDGIASPG
EILRPQDIYL QKECPVDPNT TSHPSQIQYR TRNESYKGAG GEPVVVDRVA LCSDNDRNLT
VKFMIRHTRR PEVGDKFSSR HGQKGVCGTI VQQEDLPFSE RGVCPDLIMN PHGFPSRMTV
GKMLELLGSK AAVSCGKFHY GSAFGEPSGH AHKVEDISET LVKHGFCYDG KDFLYSGTTG
TPLQAYIFMG PIYYQKLKHM VFDKMHARGI GPKTQLTRQP TEGRAKNGGL RVGEMERDCL
IAYGASNLAY ERLMLSSDPF NAQVCRKCGL LGYINYQTKT KICSTCKTGD NISDMKLPYA
CKLLFQELQS MNIVPRLQLN DS
//
