ID A0A256ID50_9EURY Unreviewed; 1362 AA.
AC A0A256ID50;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 18-JUN-2025, entry version 31.
DE RecName: Full=DNA polymerase II large subunit {ECO:0000256|HAMAP-Rule:MF_00324};
DE Short=Pol II {ECO:0000256|HAMAP-Rule:MF_00324};
DE EC=2.7.7.7 {ECO:0000256|HAMAP-Rule:MF_00324};
DE AltName: Full=Exodeoxyribonuclease large subunit {ECO:0000256|HAMAP-Rule:MF_00324};
DE EC=3.1.11.1 {ECO:0000256|HAMAP-Rule:MF_00324};
GN Name=polC {ECO:0000256|HAMAP-Rule:MF_00324};
GN ORFNames=DJ70_13800 {ECO:0000313|EMBL:OYR54458.1};
OS Halorubrum halodurans.
OC Archaea; Methanobacteriati; Methanobacteriota; Stenosarchaea group;
OC Halobacteria; Halobacteriales; Haloferacaceae; Halorubrum.
OX NCBI_TaxID=1383851 {ECO:0000313|EMBL:OYR54458.1, ECO:0000313|Proteomes:UP000216308};
RN [1] {ECO:0000313|EMBL:OYR54458.1, ECO:0000313|Proteomes:UP000216308}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Cb34 {ECO:0000313|EMBL:OYR54458.1,
RC ECO:0000313|Proteomes:UP000216308};
RX PubMed=24782836; DOI=10.3389/fmicb.2014.00140;
RA Fullmer M.S., Soucy S.M., Swithers K.S., Makkay A.M., Wheeler R.,
RA Ventosa A., Gogarten J.P., Papke R.T.;
RT "Population and genomic analysis of the genus Halorubrum.";
RL Front. Microbiol. 5:140-140(2014).
CC -!- FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an
CC exonucleolytic activity that degrades single-stranded DNA in the 3'- to
CC 5'-direction. Has a template-primer preference which is characteristic
CC of a replicative DNA polymerase. {ECO:0000256|ARBA:ARBA00025068,
CC ECO:0000256|HAMAP-Rule:MF_00324}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) +
CC diphosphate; Xref=Rhea:RHEA:22508, Rhea:RHEA-COMP:17339, Rhea:RHEA-
CC COMP:17340, ChEBI:CHEBI:33019, ChEBI:CHEBI:61560, ChEBI:CHEBI:173112;
CC EC=2.7.7.7; Evidence={ECO:0000256|ARBA:ARBA00049244,
CC ECO:0000256|HAMAP-Rule:MF_00324};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Exonucleolytic cleavage in the 3'- to 5'-direction to yield
CC nucleoside 5'-phosphates.; EC=3.1.11.1; Evidence={ECO:0000256|HAMAP-
CC Rule:MF_00324};
CC -!- SUBUNIT: Heterodimer of a large subunit and a small subunit.
CC {ECO:0000256|ARBA:ARBA00011315, ECO:0000256|HAMAP-Rule:MF_00324}.
CC -!- SIMILARITY: Belongs to the archaeal DNA polymerase II family.
CC {ECO:0000256|ARBA:ARBA00011053, ECO:0000256|HAMAP-Rule:MF_00324}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:OYR54458.1}.
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DR EMBL; NHPJ01000121; OYR54458.1; -; Genomic_DNA.
DR RefSeq; WP_094533973.1; NZ_NHPJ01000121.1.
DR OrthoDB; 7529at2157; -.
DR Proteomes; UP000216308; Unassembled WGS sequence.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008310; F:single-stranded DNA 3'-5' DNA exonuclease activity; IEA:UniProtKB-EC.
DR GO; GO:0006308; P:DNA catabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0006261; P:DNA-templated DNA replication; IEA:UniProtKB-UniRule.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR CDD; cd00350; rubredoxin_like; 1.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1.
DR HAMAP; MF_00324; DNApol_II_L_arch; 1.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004475; PolC_DP2.
DR InterPro; IPR056172; PolC_DP2_cat_dom.
DR InterPro; IPR056171; PolC_DP2_central_dom.
DR InterPro; IPR016033; PolC_DP2_N.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR NCBIfam; TIGR00354; polC; 1.
DR NCBIfam; NF011302; PRK14714.1; 1.
DR PANTHER; PTHR42210; DNA POLYMERASE II LARGE SUBUNIT; 1.
DR PANTHER; PTHR42210:SF1; DNA POLYMERASE II LARGE SUBUNIT; 1.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF24846; PolC_DP2_cat; 2.
DR Pfam; PF24844; PolC_DP2_central; 1.
DR Pfam; PF03833; PolC_DP2_N; 1.
DR PIRSF; PIRSF016275; PolC_DP2; 2.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 3: Inferred from homology;
KW DNA replication {ECO:0000256|ARBA:ARBA00022705, ECO:0000256|HAMAP-
KW Rule:MF_00324};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|HAMAP-
KW Rule:MF_00324};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932,
KW ECO:0000256|HAMAP-Rule:MF_00324};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839, ECO:0000256|HAMAP-
KW Rule:MF_00324};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|HAMAP-Rule:MF_00324};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268, ECO:0000256|HAMAP-
KW Rule:MF_00324};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722, ECO:0000256|HAMAP-Rule:MF_00324};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695, ECO:0000256|HAMAP-
KW Rule:MF_00324};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|HAMAP-
KW Rule:MF_00324}.
FT DOMAIN 933..1055
FT /note="Hint"
FT /evidence="ECO:0000259|SMART:SM00306"
FT REGION 277..332
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1048..1072
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 285..319
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1362 AA; 150002 MW; EF6F57C844EDF54F CRC64;
MRADDERYFA RLEERLDEAF EVAGTAKAQG KDPKPEIEIP VARDMADRVE NILGIDGVAE
RVRELEGEMS REEAALELVT DFVEGTVGDY DSRAGKIEGA VRTAVALLTE GVVAAPIEGI
DRVELLENDD GTEFINVYYA GPIRSAGGTA QALSVLVADY ARALLGIDEY HARTEEVERY
AEEIALYDRE TGLQYTPKDE EAKFIAENIP IMLDGEATGD EEVSGFRDLE RVDTNSARGG
MCLVAAEGIA LKAPKIQRYT RDLEEVDWPW LQDLIDGTIG KDGGDGEEEG GGDDAGEGDG
GDDGTTDGNA DEASGDDAAD PAGPPRPKPS RKFLRDLIAG RPVFTHPSEA GGFRLRYGRA
RNHGFATAGV HPATMHLVDD FLATGTQIKT ERPGKAAGVV PVDSIEGPTV KLANGEVRRI
DDPEEALAVR NGVEEILDLG EYLVNYGEFV ENNHPLAPAS YVYEWWVQEF AAAGADVQAL
ADDPNVDLER PEPGAALEWA EAYDCPLHPA YTYLWHDVSV ADVEALAEAV AGGEVVEGVL
AIEHTERVAR ALETLLVEHA ATPDALRIPT WRPLARSLGV DDGLRKEWTP EDLPREAREW
DGGDDAVKAV NAVAPFAVRE RAPVRIGNRM GRPEKSESRD LSPAVHTLFP INEAGGPQRD
VAEAARTMDD RGRRGHLELE VADRACPDCG THTYRSACPD CGTHTEPHYE CGDCGTVCEP
DEAGRVECPR CEWEVTAATR QEVDLNDAYR SALESVGERE SAFEILKGVQ GLTSTNKTPE
PIEKGILRAK NGVTSFKDGT VRYDMTDLPV TSVRPEELDV TADHFRELGY ETDIDGDPLR
HDDQLVELKV QDVVLSDGAA EHMLRTADFV DDLLEQFYDL PRFYEVNERD DLVGELVFGM
APHTSAATVG RVIGFTSAAV GYAHPYFHAA KRRNCFHPAT TIAYLEEGER REISIEAFVE
SRLGDPETDD FGTLVQELDG DVRVPSIDER GNGTTRSVTA VSKHPSQDHF VRIRTRSGRT
IRVTPDHTML RVADGEIRRT PARELAVGDR VPAAHSGERE QSTDASAASA DGGVDVDEIV
EAELVESGVE YTYNITVSDT HRLSANELWV EQCDGDEDCV MLLMDGLLNF SKTFLPNQRG
GKMDAPLVMS SRIDPSEIDD EAHNMDIVRR YPLEFYEATR EMADPEVVED RIKLGEDTLG
TDDEYRGFDH THDTTDIAMG PDLSAYKTLG DMMEKMNAQL ELARKLRAVD ETDVAERVIE
YHFLPDIIGN LRAFSRQETR CLDCGEKYRR MPLSGDCREC GGRVNLTVHE GSVSKYVDTA
IEVAERFDCR PYTKQRLYVL EDALESIFED DTNKQSGIAD FM
//
