ID A0A267F932_9PLAT Unreviewed; 457 AA.
AC A0A267F932;
DT 22-NOV-2017, integrated into UniProtKB/TrEMBL.
DT 22-NOV-2017, sequence version 1.
DT 02-APR-2025, entry version 24.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
DE Flags: Fragment;
GN ORFNames=BOX15_Mlig021995g1 {ECO:0000313|EMBL:PAA70295.1};
OS Macrostomum lignano.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes;
OC Rhabditophora; Macrostomorpha; Macrostomida; Macrostomidae; Macrostomum.
OX NCBI_TaxID=282301 {ECO:0000313|EMBL:PAA70295.1, ECO:0000313|Proteomes:UP000215902};
RN [1] {ECO:0000313|EMBL:PAA70295.1, ECO:0000313|Proteomes:UP000215902}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DV1 {ECO:0000313|EMBL:PAA70295.1};
RC TISSUE=Whole organism {ECO:0000313|EMBL:PAA70295.1};
RA Berezikov E.;
RT "A platform for efficient transgenesis in Macrostomum lignano, a flatworm
RT model organism for stem cell research.";
RL Submitted (JUN-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PAA70295.1}.
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DR EMBL; NIVC01001252; PAA70295.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A267F932; -.
DR STRING; 282301.A0A267F932; -.
DR OrthoDB; 10009520at2759; -.
DR Proteomes; UP000215902; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd20342; BRcat_RBR_RNF217; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR047551; BRcat_RBR_RNF217.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 1.
DR Pfam; PF22191; IBR_1; 1.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 3.
DR PROSITE; PS51873; TRIAD; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000215902};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT TRANSMEM 323..349
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 86..302
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
FT NON_TER 1
FT /evidence="ECO:0000313|EMBL:PAA70295.1"
SQ SEQUENCE 457 AA; 49750 MW; 445AC24FB1B7CD6C CRC64;
EAAASQPAEE LLDPHELTSV PRQSLQRWLA KSLRGQVRCG PPSLSNLAQY PLPYRAIAAH
LAATDGKAEW QTDAHNAIVP LGDLCIGLVC DICLACVSTF ARPCCAKIYC ARCLTAYALE
TMRQAQTTVG CPNLLCDKIM PQAEVMCRLP SDERWRYQRF LDNSRANGPL RKTCPGCACP
TELSAKAWRR PLQVECSSCG LVWCFPCHAP MHRGVTCRQF RASDQELMAW SKRCDAAQGA
QRNARQCPGC GAYIQKSQGC DSMTCSQCGT RFCYLCGQRY ARCPLLGSHK SRMSVMGCRY
NLLPDRPHLR RFVRGCVLAV QLIAAPALLV LGLGTALVGS GVLAAYAAFT ALRRRRLRRA
LRRKAQLSRD AFCVRAAAAA AAADAYSVST TTAERRVTIV SGNSPAAATD GSSSPATMAQ
YYCCVTTVML HEDDDSDSDD GTIVPEWLFA KSPVAES
//
