ID A0A2G5E0V4_AQUCA Unreviewed; 720 AA.
AC A0A2G5E0V4;
DT 31-JAN-2018, integrated into UniProtKB/TrEMBL.
DT 31-JAN-2018, sequence version 1.
DT 28-JAN-2026, entry version 32.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=AQUCO_01300291v1 {ECO:0000313|EMBL:PIA49365.1};
OS Aquilegia coerulea (Rocky mountain columbine).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Ranunculales; Ranunculaceae; Thalictroideae;
OC Aquilegia.
OX NCBI_TaxID=218851 {ECO:0000313|EMBL:PIA49365.1, ECO:0000313|Proteomes:UP000230069};
RN [1] {ECO:0000313|EMBL:PIA49365.1, ECO:0000313|Proteomes:UP000230069}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Goldsmith {ECO:0000313|Proteomes:UP000230069};
RA Hodges S., Kramer E., Nordborg M., Tomkins J., Borevitz J., Derieg N.,
RA Yan J., Mihaltcheva S., Hayes R.D., Rokhsar D.;
RT "WGS assembly of Aquilegia coerulea Goldsmith.";
RL Submitted (SEP-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR EMBL; KZ305030; PIA49365.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2G5E0V4; -.
DR OrthoDB; 4062651at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000230069; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd16655; RING-Ubox_WDSUB1-like; 1.
DR CDD; cd14066; STKc_IRAK; 1.
DR FunFam; 3.30.200.20:FF:000039; receptor-like protein kinase FERONIA; 1.
DR Gene3D; 3.30.200.20; Phosphorylase Kinase, domain 1; 1.
DR Gene3D; 1.10.510.10; Transferase(Phosphotransferase) domain 1; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR InterPro; IPR051348; U-box_ubiquitin_ligases.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR45647; OS02G0152300 PROTEIN; 1.
DR PANTHER; PTHR45647:SF100; U-BOX DOMAIN-CONTAINING PROTEIN 33; 1.
DR Pfam; PF00069; Pkinase; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00220; S_TKc; 1.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Coiled coil {ECO:0000256|SAM:Coils};
KW Kinase {ECO:0000256|ARBA:ARBA00022777};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU10141}; Reference proteome {ECO:0000313|Proteomes:UP000230069};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT DOMAIN 361..631
FT /note="Protein kinase"
FT /evidence="ECO:0000259|PROSITE:PS50011"
FT DOMAIN 649..720
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
FT REGION 130..184
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 242..332
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 130..147
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 170..184
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 388
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU10141"
SQ SEQUENCE 720 AA; 80558 MW; A61D443BEE159F28 CRC64;
MLKSKKAIFV NQEAPISCHI WFVCKGSLIQ TREGSLEGME IRITDSPLAS PLTETSHGDC
LRSRSISEGQ SQHVKLTSPV QNFLNRLRSH GRRVATVPSL DVNKGVLLPQ HQLGAEESVN
AWEGMSQGSV LSTWSSSEEQ IGSLESNSEV RDEASEDDSL LSSIHESEED ARRTSPTHEL
DPGTLDRELI DLLQQAMAEA ENSKREAFDE LVKRRRAEKD VIEAIRRVKE SESLYANKVK
RKKEMEVVLA RETSELNKIN SQKDKVMEEL KTALDQKMAL ESQLADSGRM LEEFKEKMAV
AVELLQTFKQ QRDSLQFERD SAVNEAEELR KKRDTEASSS QSQQIFSEFS FSEIDAATVN
FDPRLVIGQG GYGSVYKGVL RNTEVAIKLL NSSSKQGRSE FQQEVDVLSK MRHPNLLTLI
GTCPEAWALI YEYLPNGSVE DRLTCKDNTA PLSWQTRIRI AVEICSALIF LHYNKPHGIV
HGDLKPSNIL LDANFISKLG DFGICRSLSP DDISTYTTTL CSRTEHPKGT LLYMDPQFLS
TGELTAKSDV YSFGIVLLQL LTGRPALGIA KEVQCALDEG TLNALLDCSA GDWPFVQAKQ
LAFLALRCCE MNRKNRPDLV SEVWRVLEPM KAACASSSSF RLGSEEPCQI PSYFICPIFQ
EIMREPRIAA DGFTYEAEAI KGWLDGGHNT SPMTNLKLAN CDLIPNHSLR SAIEEWRQQL
//
