ID A0A2U1NNG6_ARTAN Unreviewed; 622 AA.
AC A0A2U1NNG6;
DT 18-JUL-2018, integrated into UniProtKB/TrEMBL.
DT 18-JUL-2018, sequence version 1.
DT 28-JAN-2026, entry version 26.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=CTI12_AA246080 {ECO:0000313|EMBL:PWA75026.1};
OS Artemisia annua (Sweet wormwood).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; campanulids; Asterales; Asteraceae; Asteroideae; Anthemideae;
OC Artemisiinae; Artemisia.
OX NCBI_TaxID=35608 {ECO:0000313|EMBL:PWA75026.1, ECO:0000313|Proteomes:UP000245207};
RN [1] {ECO:0000313|EMBL:PWA75026.1, ECO:0000313|Proteomes:UP000245207}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Huhao1 {ECO:0000313|Proteomes:UP000245207};
RC TISSUE=Leaf {ECO:0000313|EMBL:PWA75026.1};
RX PubMed=29703587; DOI=10.1016/j.molp.2018.03.015;
RA Shen Q., Zhang L., Liao Z., Wang S., Yan T., Shi P., Liu M., Fu X., Pan Q.,
RA Wang Y., Lv Z., Lu X., Zhang F., Jiang W., Ma Y., Chen M., Hao X., Li L.,
RA Tang Y., Lv G., Zhou Y., Sun X., Brodelius P.E., Rose J.K.C., Tang K.;
RT "The genome of Artemisia annua provides insight into the evolution of
RT Asteraceae family and artemisinin biosynthesis.";
RL Mol. Plant 11:776-788(2018).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|ARBA:ARBA00003861}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PWA75026.1}.
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DR EMBL; PKPP01002471; PWA75026.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2U1NNG6; -.
DR STRING; 35608.A0A2U1NNG6; -.
DR OrthoDB; 7537227at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000245207; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR CDD; cd16664; RING-Ubox_PUB; 1.
DR FunFam; 1.25.10.10:FF:000082; RING-type E3 ubiquitin transferase; 1.
DR FunFam; 3.30.40.10:FF:000292; RING-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR058678; ARM_PUB.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR057623; PUB12-19-like_N.
DR InterPro; IPR045210; RING-Ubox_PUB.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR23315:SF342; RING-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR PANTHER; PTHR23315; U BOX DOMAIN-CONTAINING; 1.
DR Pfam; PF25598; ARM_PUB; 1.
DR Pfam; PF25368; PUB10_N; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 6.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS50176; ARM_REPEAT; 2.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054};
KW Reference proteome {ECO:0000313|Proteomes:UP000245207};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT DOMAIN 233..307
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
FT REPEAT 374..416
FT /note="ARM"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00259"
FT REPEAT 415..457
FT /note="ARM"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00259"
FT REGION 150..176
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 155..164
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 622 AA; 68353 MW; 6E8DEEDE2675123F CRC64;
MQSPLMARRD VENSNAMITE IQSLLCDVSK SSCIGFTGVF KKDCVDLSRR VALLYYLLEE
IKDFEVPVGS LSELMEAIKS VEGLVVSAGR FDHDMSSDGA AQKIVLQFHS VTWKLEKALA
NLPYDQFDIS EEVKEQVDLV RGQLKRATKR YCGPQDSTSH SCGVSDSLAE ESGSTQAIVN
DHQEVESKVE VIEHETHSND SDDMIDQVER SRIESAKASS DVSGLSKPSG STVIPDDFLC
PISLELMKDP VIVSTGQTYE RLYIQRWINC GNTTCPKTRQ KLQNMTLTPN YVLRSLITQW
CTDHNVDHPT LLANRRLKKS DGTCLDVSQD IDLIEAIVLN LSSHSIEEQR TAASKIRSLS
KRSTDNRILI AEAGAIPILV ELLTSEDAIT LEHVVTSMLN LSIYDNNREL IMLANAVPSL
VQVLKTGTME TRENAAATLF SLSLDDENKI IIGASGAIPL LVDLLRSGSK RGKNDAATAL
FNLCIYQGNK GKAVRAGIIF VLINMLTESN GLMVDEALTI LSILASHQEA KAAMVRACVI
PNLVNHLTSG NSRNKENATS ILLSLCKRDN DNLGFISRLG AVIPLTEIAE SGTERAQRKA
SSLLGYLQRF QDSQQQPPEQ VD
//
