ID A0A2Z6P1Z9_TRISU Unreviewed; 387 AA.
AC A0A2Z6P1Z9;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 08-OCT-2025, entry version 23.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=TSUD_136810 {ECO:0000313|EMBL:GAU42295.1};
OS Trifolium subterraneum (Subterranean clover).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Trifolieae; Trifolium.
OX NCBI_TaxID=3900 {ECO:0000313|EMBL:GAU42295.1, ECO:0000313|Proteomes:UP000242715};
RN [1] {ECO:0000313|Proteomes:UP000242715}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Daliak {ECO:0000313|Proteomes:UP000242715};
RX PubMed=28928752; DOI=10.3389/fpls.2017.01463;
RA Kaur P., Appels R., Bayer P.E., Keeble-Gagnere G., Wang J., Hirakawa H.,
RA Shirasawa K., Vercoe P., Stefanova K., Durmic Z., Nichols P., Revell C.,
RA Isobe S.N., Edwards D., Erskine W.;
RT "Climate Clever Clovers: New Paradigm to Reduce the Environmental Footprint
RT of Ruminants by Breeding Low Methanogenic Forages Utilizing Haplotype
RT Variation.";
RL Front. Plant Sci. 8:1463-1463(2017).
CC -!- FUNCTION: Functions as an E3 ubiquitin ligase.
CC {ECO:0000256|ARBA:ARBA00003861}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
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DR EMBL; DF973909; GAU42295.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A2Z6P1Z9; -.
DR OrthoDB; 7537227at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000242715; Unassembled WGS sequence.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR FunFam; 3.30.40.10:FF:000565; RING-type E3 ubiquitin transferase; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR000225; Armadillo.
DR InterPro; IPR003613; Ubox_domain.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR23315; U BOX DOMAIN-CONTAINING; 1.
DR PANTHER; PTHR23315:SF276; U-BOX DOMAIN-CONTAINING PROTEIN 38; 1.
DR Pfam; PF00514; Arm; 1.
DR Pfam; PF04564; U-box; 1.
DR SMART; SM00185; ARM; 2.
DR SMART; SM00504; Ubox; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS51698; U_BOX; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000242715};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786}.
FT DOMAIN 25..99
FT /note="U-box"
FT /evidence="ECO:0000259|PROSITE:PS51698"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..17
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 387 AA; 42520 MW; 429B4D5312C77A28 CRC64;
MGGNGKHRWK ISFHRSSSHS SRNSQPPKEF LCPITGSLMS DPVVVTSGQT FERLAVQIST
EFKFSPKLED GTRPDFSTII PNLAIKTTIT NWCDKSRTQY PPAPDYTTVE KLIKEAMAAE
KEDPIRTSEK ELLNAVADNP PVIFSHAATE LGPRINRFNS GSSSEESEHA AGALFSLALD
DDNKMAIGVL GALQPLMHAL RSDSERTRHD SALALYHLTL VQSNRVKLVK LGVVPTLLSM
VMTGTMASRV LLILCNLAMS VEGRTAMLDA NAVECMVSLL RGNELDSEAT RENCVAALYA
LSHGSLRFKG LAKEAKAVEV LRVIEETGTE RAREKAKRVL QKMRGFEDGD DEDDSGFDSL
FESSRLTRTR YRGAGARNNN LVNSTTF
//
