ID A0A318ZTN6_9EURO Unreviewed; 500 AA.
AC A0A318ZTN6;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 28-JAN-2026, entry version 24.
DE RecName: Full=DNA primase large subunit {ECO:0000256|PIRNR:PIRNR009449};
GN ORFNames=BP01DRAFT_352572 {ECO:0000313|EMBL:PYH50034.1};
OS Aspergillus saccharolyticus JOP 1030-1.
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Circumdati.
OX NCBI_TaxID=1450539 {ECO:0000313|EMBL:PYH50034.1, ECO:0000313|Proteomes:UP000248349};
RN [1] {ECO:0000313|EMBL:PYH50034.1, ECO:0000313|Proteomes:UP000248349}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JOP 1030-1 {ECO:0000313|EMBL:PYH50034.1,
RC ECO:0000313|Proteomes:UP000248349};
RG DOE Joint Genome Institute;
RA Vesth T.C., Nybo J., Theobald S., Brandl J., Frisvad J.C., Nielsen K.F.,
RA Lyhne E.K., Kogle M.E., Kuo A., Riley R., Clum A., Nolan M., Lipzen A.,
RA Salamov A., Henrissat B., Wiebenga A., De Vries R.P., Grigoriev I.V.,
RA Mortensen U.H., Andersen M.R., Baker S.E.;
RT "The genomes of Aspergillus section Nigri reveals drivers in fungal
RT speciation.";
RL Submitted (DEC-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: DNA primase is the polymerase that synthesizes small RNA
CC primers for the Okazaki fragments made during discontinuous DNA
CC replication. {ECO:0000256|PIRNR:PIRNR009449}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|PIRNR:PIRNR009449};
CC Note=Binds 1 [4Fe-4S] cluster. {ECO:0000256|PIRNR:PIRNR009449};
CC -!- SIMILARITY: Belongs to the eukaryotic-type primase large subunit
CC family. {ECO:0000256|ARBA:ARBA00010564, ECO:0000256|PIRNR:PIRNR009449}.
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DR EMBL; KZ821218; PYH50034.1; -; Genomic_DNA.
DR RefSeq; XP_025436016.1; XM_025573974.1.
DR AlphaFoldDB; A0A318ZTN6; -.
DR STRING; 1450539.A0A318ZTN6; -.
DR GeneID; 37075202; -.
DR OrthoDB; 421393at2759; -.
DR Proteomes; UP000248349; Unassembled WGS sequence.
DR GO; GO:0005658; C:alpha DNA polymerase:primase complex; IEA:TreeGrafter.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006270; P:DNA replication initiation; IEA:TreeGrafter.
DR GO; GO:0006269; P:DNA replication, synthesis of primer; IEA:UniProtKB-KW.
DR CDD; cd07322; PriL_PriS_Eukaryotic; 1.
DR FunFam; 1.20.930.80:FF:000003; DNA primase large subunit; 1.
DR Gene3D; 1.20.930.80; -; 1.
DR InterPro; IPR058560; DNA_primase_C.
DR InterPro; IPR016558; DNA_primase_lsu_euk.
DR InterPro; IPR007238; DNA_primase_lsu_euk/arc.
DR PANTHER; PTHR10537; DNA PRIMASE LARGE SUBUNIT; 1.
DR PANTHER; PTHR10537:SF3; DNA PRIMASE LARGE SUBUNIT; 1.
DR Pfam; PF04104; DNA_primase_lrg; 1.
DR Pfam; PF26466; DNA_primase_lrg_N; 1.
DR PIRSF; PIRSF009449; DNA_primase_large_subunit; 1.
DR SUPFAM; SSF140914; PriB N-terminal domain-like; 1.
PE 3: Inferred from homology;
KW 4Fe-4S {ECO:0000256|ARBA:ARBA00022485, ECO:0000256|PIRNR:PIRNR009449};
KW DNA replication {ECO:0000256|ARBA:ARBA00022705,
KW ECO:0000256|PIRNR:PIRNR009449};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125, ECO:0000256|PIRNR:PIRNR009449};
KW Iron {ECO:0000256|ARBA:ARBA00023004, ECO:0000256|PIRNR:PIRNR009449};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014, ECO:0000256|PIRNR:PIRNR009449};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRNR:PIRNR009449};
KW Primosome {ECO:0000256|ARBA:ARBA00022515, ECO:0000256|PIRNR:PIRNR009449};
KW Reference proteome {ECO:0000313|Proteomes:UP000248349}.
FT DOMAIN 301..484
FT /note="DNA primase large subunit C-terminal"
FT /evidence="ECO:0000259|Pfam:PF04104"
FT BINDING 307
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 390
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 407
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
FT BINDING 448
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000256|PIRSR:PIRSR009449-1"
SQ SEQUENCE 500 AA; 58398 MW; 4D268DFF1A617E23 CRC64;
MIRGDFLRID PKRRANLDHK KKQFAAPTFK QQDYPHRLNF YDVPPTEEIT LEQFEQWAID
RLRVLAEIEA CSYRNKSAAE TEAHLTPLLQ KYLPLSSNTS SSHGAADQRL KNERQKDHYS
HFILRLAFSA TEDLRRRFVR AETMLFRFRF QRDDTREKRA FIESLNLDWE PVENEERQRL
AENLVNATPG LRRVDEEAWY KVDWERVPEL VERRAVYLYK GKAYVPGREQ LSMIIAEFTA
RLERALELTS RALPRLDEDD RLTPILNHLS KNFGSAESVY SEGEGFVDGA AITAGNIDQL
SQHFPLCMRS LHMNLRKNNH LKHYGRLQYT LFLKGIGLSL EECIVFWRQS FKGFTDDEFN
SRYKYNIRHA YGDVGGDVNR RGRGYPPYSC QKILGDSNPG VGQTHGCPYR HYSIDNLIGL
LQSTGVHDKE VLRGVREDVE KMRYHIACNR VFESTHKTEI KRVKEDGSWN QTDLDTIVHP
NTYFKRSFLL KQTARASKNS
//
