ID A0A323TK50_9BACI Unreviewed; 632 AA.
AC A0A323TK50;
DT 10-OCT-2018, integrated into UniProtKB/TrEMBL.
DT 10-OCT-2018, sequence version 1.
DT 28-JAN-2026, entry version 28.
DE RecName: Full=Selenocysteine-specific elongation factor {ECO:0000256|ARBA:ARBA00015953};
DE AltName: Full=SelB translation factor {ECO:0000256|ARBA:ARBA00031615};
GN Name=selB {ECO:0000313|EMBL:PYZ95278.1};
GN ORFNames=CR194_07140 {ECO:0000313|EMBL:PYZ95278.1};
OS Salipaludibacillus keqinensis.
OC Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae.
OX NCBI_TaxID=2045207 {ECO:0000313|EMBL:PYZ95278.1, ECO:0000313|Proteomes:UP000248214};
RN [1] {ECO:0000313|EMBL:PYZ95278.1, ECO:0000313|Proteomes:UP000248214}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KQ-12 {ECO:0000313|EMBL:PYZ95278.1,
RC ECO:0000313|Proteomes:UP000248214};
RA Wang H.;
RT "Bacillus sp. nov., a halophilic bacterium isolated from a Keqin Lake.";
RL Submitted (OCT-2017) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Translation factor necessary for the incorporation of
CC selenocysteine into proteins. It probably replaces EF-Tu for the
CC insertion of selenocysteine directed by the UGA codon. SelB binds GTP
CC and GDP. {ECO:0000256|ARBA:ARBA00025526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:PYZ95278.1}.
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DR EMBL; PDOD01000001; PYZ95278.1; -; Genomic_DNA.
DR RefSeq; WP_110608913.1; NZ_PDOD01000001.1.
DR AlphaFoldDB; A0A323TK50; -.
DR OrthoDB; 9804504at2; -.
DR Proteomes; UP000248214; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0003924; F:GTPase activity; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR GO; GO:0003746; F:translation elongation factor activity; IEA:UniProtKB-KW.
DR GO; GO:0001514; P:selenocysteine incorporation; IEA:InterPro.
DR CDD; cd04171; SelB; 1.
DR CDD; cd03696; SelB_II; 1.
DR CDD; cd15491; selB_III; 1.
DR Gene3D; 1.10.10.2770; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 2.40.30.10; Translation factors; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR InterPro; IPR057335; Beta-barrel_SelB.
DR InterPro; IPR050055; EF-Tu_GTPase.
DR InterPro; IPR004161; EFTu-like_2.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015191; SelB_WHD4.
DR InterPro; IPR005225; Small_GTP-bd.
DR InterPro; IPR000795; T_Tr_GTP-bd_dom.
DR InterPro; IPR009000; Transl_B-barrel_sf.
DR InterPro; IPR009001; Transl_elong_EF1A/Init_IF2_C.
DR InterPro; IPR004535; Transl_elong_SelB.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR NCBIfam; TIGR00475; selB; 1.
DR NCBIfam; TIGR00231; small_GTP; 1.
DR PANTHER; PTHR43721; ELONGATION FACTOR TU-RELATED; 1.
DR PANTHER; PTHR43721:SF11; SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR; 1.
DR Pfam; PF25461; Beta-barrel_SelB; 1.
DR Pfam; PF00009; GTP_EFTU; 1.
DR Pfam; PF03144; GTP_EFTU_D2; 1.
DR Pfam; PF09107; WHD_3rd_SelB; 1.
DR PRINTS; PR00315; ELONGATNFCT.
DR SUPFAM; SSF50465; EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50447; Translation proteins; 1.
DR SUPFAM; SSF46785; Winged helix' DNA-binding domain; 1.
DR PROSITE; PS51722; G_TR_2; 1.
PE 4: Predicted;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Elongation factor {ECO:0000313|EMBL:PYZ95278.1};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000248214}.
FT DOMAIN 3..175
FT /note="Tr-type G"
FT /evidence="ECO:0000259|PROSITE:PS51722"
SQ SEQUENCE 632 AA; 71709 MW; 440FA107D962F728 CRC64;
MSKRFYTIGM AGHIDHGKTT LTKALTNIDT DRLKEEKERA ISIELGYAPL TLDDESQVSV
IDVPGHEKFI RQMIAGVAGI DLVLLVVAAD EGVMPQTVEH LDILNLLGIH QGLIVVTKMD
QVEEEMIELI EADISDQVEG TFFEGSELVF VDSLSQKGIP ELREIIKQKL STIPVRDAKG
AFRLPIDQVF SVHGQGTVVR GTVYEGEVRE GDTLEILPQQ KKVRARQLQV HHEPQKSGRA
GQRVAVNLGG ISKHELNRGD VLVSTQHYST TNTIDISLDT VDQLKYPVKQ RGYIKLHIGT
AEVYGKIVFF DRNELSEDKN VLCQLRLDDP VVTKRGDRFI LRRPSPMETI GGGVVIDPQG
EKYKFGEATI EKLQRKKEGS PEERLMDLLK LKKALSKKEV CAELSLSDDD FNEFVNPLLE
EGTLLRFGNH YLLAKTYEEM LEIIEEELLT YHNEFPLRDG MELAQLKQDL NPYGKEIASS
VIDQAITDGR MKKRGSAVAS TTFEPGYPKQ WEKRMKQVEE TLMDQQLEVE PFSVILERAG
LPDQWAQELK HFLVRQQWMK ELDEKHLVSA KSFTKALSDL YDQTGERFTL QEAKQTLGLT
RKYLVPLMEL VDKSGYTKRE DQERVWVKEP GK
//
