ID A0A3A4KS85_9NOCA Unreviewed; 748 AA.
AC A0A3A4KS85;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 18-JUN-2025, entry version 29.
DE RecName: Full=DNA ligase (ATP) {ECO:0000256|ARBA:ARBA00012727};
DE EC=6.5.1.1 {ECO:0000256|ARBA:ARBA00012727};
DE AltName: Full=NHEJ DNA polymerase {ECO:0000256|ARBA:ARBA00029943};
GN ORFNames=D5S18_04710 {ECO:0000313|EMBL:RJO78826.1};
OS Nocardia panacis.
OC Bacteria; Bacillati; Actinomycetota; Actinomycetes; Mycobacteriales;
OC Nocardiaceae; Nocardia.
OX NCBI_TaxID=2340916 {ECO:0000313|EMBL:RJO78826.1, ECO:0000313|Proteomes:UP000266677};
RN [1] {ECO:0000313|EMBL:RJO78826.1, ECO:0000313|Proteomes:UP000266677}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=YIM PH 21724 {ECO:0000313|EMBL:RJO78826.1,
RC ECO:0000313|Proteomes:UP000266677};
RA Miao C.;
RT "YIM PH21274 draft genome.";
RL Submitted (SEP-2018) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-
CC (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP +
CC diphosphate.; EC=6.5.1.1; Evidence={ECO:0000256|ARBA:ARBA00034003};
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SIMILARITY: In the C-terminal section; belongs to the ATP-dependent DNA
CC ligase family. {ECO:0000256|ARBA:ARBA00049981}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the LigD polymerase
CC family. {ECO:0000256|ARBA:ARBA00049990}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RJO78826.1}.
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DR EMBL; QZFU01000012; RJO78826.1; -; Genomic_DNA.
DR RefSeq; WP_120038324.1; NZ_QZFU01000012.1.
DR AlphaFoldDB; A0A3A4KS85; -.
DR OrthoDB; 9802472at2; -.
DR Proteomes; UP000266677; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003910; F:DNA ligase (ATP) activity; IEA:UniProtKB-EC.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0004527; F:exonuclease activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR CDD; cd07906; Adenylation_DNA_ligase_LigD_LigC; 1.
DR CDD; cd04863; MtLigD_Pol_like; 1.
DR CDD; cd07971; OBF_DNA_ligase_LigD; 1.
DR Gene3D; 3.30.1490.70; -; 1.
DR Gene3D; 3.30.470.30; DNA ligase/mRNA capping enzyme; 1.
DR Gene3D; 3.90.920.10; DNA primase, PRIM domain; 1.
DR Gene3D; 2.40.50.140; Nucleic acid-binding proteins; 1.
DR InterPro; IPR012309; DNA_ligase_ATP-dep_C.
DR InterPro; IPR012310; DNA_ligase_ATP-dep_cent.
DR InterPro; IPR014146; LigD_ligase_dom.
DR InterPro; IPR014144; LigD_PE_domain.
DR InterPro; IPR014145; LigD_pol_dom.
DR InterPro; IPR033649; MtLigD_Pol-like.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR052171; NHEJ_LigD.
DR NCBIfam; TIGR02777; LigD_PE_dom; 1.
DR NCBIfam; TIGR02778; ligD_pol; 1.
DR NCBIfam; TIGR02779; NHEJ_ligase_lig; 1.
DR NCBIfam; NF007210; PRK09632.1; 1.
DR PANTHER; PTHR42705; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR PANTHER; PTHR42705:SF2; BIFUNCTIONAL NON-HOMOLOGOUS END JOINING PROTEIN LIGD; 1.
DR Pfam; PF04679; DNA_ligase_A_C; 1.
DR Pfam; PF01068; DNA_ligase_A_M; 1.
DR Pfam; PF13298; LigD_N; 1.
DR Pfam; PF21686; LigD_Prim-Pol; 1.
DR SUPFAM; SSF56091; DNA ligase/mRNA capping enzyme, catalytic domain; 1.
DR SUPFAM; SSF50249; Nucleic acid-binding proteins; 1.
DR PROSITE; PS50160; DNA_LIGASE_A3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA damage {ECO:0000256|ARBA:ARBA00022763};
KW DNA recombination {ECO:0000256|ARBA:ARBA00023172};
KW DNA repair {ECO:0000256|ARBA:ARBA00023204};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Exonuclease {ECO:0000256|ARBA:ARBA00022839};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:RJO78826.1};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Multifunctional enzyme {ECO:0000256|ARBA:ARBA00023268};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000266677};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 549..682
FT /note="ATP-dependent DNA ligase family profile"
FT /evidence="ECO:0000259|PROSITE:PS50160"
FT REGION 298..319
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..319
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 748 AA; 83075 MW; 06DDEA13A33B3E61 CRC64;
MVARREFGEI EVELSNLDKV LYPATGTTKG EVIAYYSAIA PAMLPHIADR PVTRKRWPNG
VDSPAFFEKN LPEHAPSWIP RRAVEHSARR RVVYPLIDSV AGLAWIGQQA ALEVHVPQWR
FDGPEVGPAT RLVFDLDPGP GAGLAECALV ALGVREMIEG IGLRAYPVTS GSKGIHLYVP
LDRVLSSGGA STVAKQVAAN LERLHPDLVT ATMAKSARAG KVFLDWSQNN PAKTTIAPYS
MRGREHPNAA APRTWAEIEN RKKLRHLSFD EVLARFLDGG DLLADLDAPP PPDTLATYRS
MRDPARTPEP VPAAPPRPGP GDRFVVQEHH ARRLHWDVRL ERDGVLVSWA VPKGPPTSPA
QNRLAVHTED HPLEYLEFHG AIPKGEYGAG AMTIWDAGTY ETEKWREDEV IVRLHGRRLD
GRYAFIRTDG KQWLMHLMRD QTNGAAAQVD SLRQKVPFPR GLAPMLAVGG EVAELGDAEW
SFETKWDGFR LMAEIDSGVL TLRSRAGNVV TERYPGMGAL AAELAGHSVV LDGEAVVFDD
HGVANLGLLR ADAARAVLVA FDVLYLDGTS LVRKRYSDRR RVLEALAANA PSLRVPARLD
GPGATALRYS REQGMEGVIA KRRDSVYLPG KRGHSWVKQR NWRIQRVVVG GWRRSAAREF
KSLLVGIPHE GRLFYVGRVG TGFDESEMRE LTARLRRLER KTSPFDNELT KDERESAVWV
TPRVEGSVRF MNWTETGRLW HPAWLGTR
//
