ID A0A3B1IEM5_ASTMX Unreviewed; 254 AA.
AC A0A3B1IEM5;
DT 05-DEC-2018, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 1.
DT 18-JUN-2025, entry version 30.
DE RecName: Full=NFU1 iron-sulfur cluster scaffold homolog, mitochondrial {ECO:0000256|ARBA:ARBA00018782};
DE AltName: Full=HIRA-interacting protein 5 {ECO:0000256|ARBA:ARBA00076495};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Acestrorhamphidae; Acestrorhamphinae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000028388.1, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000028388.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- FUNCTION: Iron-sulfur cluster scaffold protein which can assemble [4Fe-
CC 4S] clusters and deliver them to target proteins.
CC {ECO:0000256|ARBA:ARBA00057718}.
CC -!- SUBUNIT: Monomer and homohexamer; the apo-NFU1 is a monomer, while the
CC holo-NFU1 is a hexamer composed of a trimer of dimer that is probably
CC linked by some 4Fe-4S cluster. Interacts with HIRA and EPM2A/laforin.
CC Interacts with BOLA3. Interacts with HSPA9.
CC {ECO:0000256|ARBA:ARBA00062506}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000256|ARBA:ARBA00004173}.
CC -!- SIMILARITY: Belongs to the NifU family.
CC {ECO:0000256|ARBA:ARBA00006420}.
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DR RefSeq; XP_007234817.1; XM_007234755.2.
DR AlphaFoldDB; A0A3B1IEM5; -.
DR FunCoup; A0A3B1IEM5; 1427.
DR STRING; 7994.ENSAMXP00000028388; -.
DR Ensembl; ENSAMXT00000049693.1; ENSAMXP00000028388.1; ENSAMXG00000036614.1.
DR GeneID; 103021881; -.
DR KEGG; amex:103021881; -.
DR CTD; 27247; -.
DR GeneTree; ENSGT00940000164785; -.
DR InParanoid; A0A3B1IEM5; -.
DR OMA; AIMEHYM; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000036614; Expressed in heart and 14 other cell types or tissues.
DR GO; GO:0005739; C:mitochondrion; IEA:TreeGrafter.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:InterPro.
DR GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:InterPro.
DR FunFam; 3.30.300.130:FF:000001; NFU1 iron-sulfur cluster scaffold; 1.
DR FunFam; 3.30.1370.70:FF:000002; NFU1 iron-sulfur cluster scaffold homolog, mitochondrial; 1.
DR Gene3D; 3.30.300.130; Fe-S cluster assembly (FSCA); 1.
DR Gene3D; 3.30.1370.70; Scaffold protein Nfu/NifU, N-terminal domain; 1.
DR InterPro; IPR034904; FSCA_dom_sf.
DR InterPro; IPR014824; Nfu/NifU_N.
DR InterPro; IPR036498; Nfu/NifU_N_sf.
DR InterPro; IPR001075; NIF_FeS_clus_asmbl_NifU_C.
DR PANTHER; PTHR11178; IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED; 1.
DR PANTHER; PTHR11178:SF46; NFU1 IRON-SULFUR CLUSTER SCAFFOLD HOMOLOG, MITOCHONDRIAL; 1.
DR Pfam; PF08712; Nfu_N; 1.
DR Pfam; PF01106; NifU; 1.
DR SMART; SM00932; Nfu_N; 1.
DR SUPFAM; SSF117916; Fe-S cluster assembly (FSCA) domain-like; 1.
DR SUPFAM; SSF110836; Hypothetical protein SAV1430; 1.
PE 3: Inferred from homology;
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00023014};
KW Mitochondrion {ECO:0000256|ARBA:ARBA00023128};
KW Reference proteome {ECO:0000313|Proteomes:UP000018467};
KW Transit peptide {ECO:0000256|ARBA:ARBA00022946}.
FT DOMAIN 59..146
FT /note="Scaffold protein Nfu/NifU N-terminal"
FT /evidence="ECO:0000259|SMART:SM00932"
SQ SEQUENCE 254 AA; 27956 MW; 148E6C18A70B3B94 CRC64;
MAAYRSSGVL RTLPPLLAAL RGPTCSYHAA VLSRPRFNTV VMRTLPQAPT SMFVRNMFIQ
TQDTPNPNSL KFLPGRIVLE SGTMDFASAR DAFCSPLARQ LFRIDGVKGV FLGPDFITIT
KTNSDAEWKV IKPDVFATIM DFFTSGLPVV SEDAVPSADT APSDDDDEVV AMIKELLDTR
IRPTVQEDGG DVLYRGFEDG IVKLKLQGSC TSCPSSIVTL KNGIQNMLQF YVPEVEGVEQ
VKEEVEDVEQ EAQR
//
