ID A0A3Q1EUY0_9TELE Unreviewed; 615 AA.
AC A0A3Q1EUY0;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 18-JUN-2025, entry version 28.
DE RecName: Full=E3 ubiquitin-protein ligase RNF220 {ECO:0000256|ARBA:ARBA00067773};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
DE AltName: Full=RING finger protein 220 {ECO:0000256|ARBA:ARBA00083046};
DE AltName: Full=RING-type E3 ubiquitin transferase RNF220 {ECO:0000256|ARBA:ARBA00079756};
OS Acanthochromis polyacanthus (spiny chromis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Pomacentridae; Acanthochromis.
OX NCBI_TaxID=80966 {ECO:0000313|Ensembl:ENSAPOP00000007854.1, ECO:0000313|Proteomes:UP000257200};
RN [1] {ECO:0000313|Ensembl:ENSAPOP00000007854.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SUBUNIT: Interacts with SIN3B. Interacts with CTNNB1 (via Armadillo
CC repeats 2-8). Interacts with USP7 (via MATH domain).
CC {ECO:0000256|ARBA:ARBA00063526}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496}.
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DR RefSeq; XP_022060795.1; XM_022205103.2.
DR AlphaFoldDB; A0A3Q1EUY0; -.
DR Ensembl; ENSAPOT00000004234.1; ENSAPOP00000007854.1; ENSAPOG00000009927.1.
DR GeneID; 110958524; -.
DR GeneTree; ENSGT00390000016573; -.
DR Proteomes; UP000257200; Unplaced.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:TreeGrafter.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:TreeGrafter.
DR CDD; cd16563; RING-HC_RNF220; 1.
DR FunFam; 3.30.40.10:FF:000195; E3 ubiquitin-protein ligase RNF220; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR052443; E3_ubiq-ligase_RNF220-like.
DR InterPro; IPR031824; RNF220_mid.
DR InterPro; IPR040178; RNF220_RING.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR13459; E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1; 1.
DR PANTHER; PTHR13459:SF1; E3 UBIQUITIN-PROTEIN LIGASE RNF220 ISOFORM X1; 1.
DR Pfam; PF15926; RNF220; 1.
DR Pfam; PF13923; zf-C3HC4_2; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 4: Predicted;
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Isopeptide bond {ECO:0000256|ARBA:ARBA00022499};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000257200};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation {ECO:0000256|ARBA:ARBA00022843};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT DOMAIN 563..602
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT REGION 490..520
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 505..520
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 615 AA; 67134 MW; D314D91396210C9F CRC64;
MDLHRAAFKM ESSSYLPNPL ASPALMVLAS TAEASRDASI PCQQPRPFGV PVSVEKDVHL
PFNNGSYTFA SMYHRQGAVP PGFPNRDFPP SLLHLHHQFA PPNLDCSPIS MLNHSGVGAF
RPFASPPEDR DGAPGGYQSA FTPAKRLKGC LDAEASPHLR YSDAEGKEYD FGGAQIPPGG
SPSSALKAVE DSGKKIFAVS GLLSDRETSS SPEDRIERCK KKMSLYDSQA PVCPICQVLL
RPGELQEHME TEIERLANIC LSKNTSPKDS AATPGTPKSM LLSVHIKREG ESPVVSPLSS
EDAHHSDRYQ TFLRVRANRQ TRLNARIGKM KRRKPEDGGQ VCPLCSAPLA GSEEEMSRHV
EQCLIQREGA LGDDDSADMD GENGRGFEEY EWAGQKRIRA TALLEGGFRG TGFATCSIKE
SAADSDADLD VDGDDTLEYG KAQYTEADII PCSGAGEDQG EAREREALRG AVLNGGMPSN
RITPEFSKWA SDEMPSTSNG ESSKTDPSTP SSSSSSCCVP RTCKNSEIEK VTEEESTATT
MEALKARIRE LEKQILRGDR YKCLICMDSY TMPLTSIQCW HVHCEECWLR TLGNKKLCPQ
CNTITSPGDL RRVYL
//
