ID A0A3Q1MX43_BOVIN Unreviewed; 851 AA.
AC A0A3Q1MX43;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 29-MAY-2024, sequence version 2.
DT 18-JUN-2025, entry version 29.
DE RecName: Full=Oxysterol-binding protein {ECO:0000256|RuleBase:RU003845};
GN Name=OSBPL3 {ECO:0000313|Ensembl:ENSBTAP00000060232.2,
GN ECO:0000313|VGNC:VGNC:32462};
OS Bos taurus (Bovine).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Bovinae; Bos.
OX NCBI_TaxID=9913 {ECO:0000313|Ensembl:ENSBTAP00000060232.2, ECO:0000313|Proteomes:UP000009136};
RN [1] {ECO:0000313|Ensembl:ENSBTAP00000060232.2, ECO:0000313|Proteomes:UP000009136}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000060232.2,
RC ECO:0000313|Proteomes:UP000009136};
RA Rosen B.D., Bickhart D.M., Koren S., Schnabel R.D., Hall R., Zimin A.,
RA Dreischer C., Schultheiss S., Schroeder S.G., Elsik C.G., Couldrey C.,
RA Liu G.E., Van Tassell C.P., Phillippy A.M., Smith T.P.L., Medrano J.F.;
RT "ARS-UCD1.2.";
RL Submitted (MAR-2018) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSBTAP00000060232.2}
RP IDENTIFICATION.
RC STRAIN=Hereford {ECO:0000313|Ensembl:ENSBTAP00000060232.2};
RG Ensembl;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- FUNCTION: Phosphoinositide-binding protein which associates with both
CC cell and endoplasmic reticulum (ER) membranes. Can bind to the ER
CC membrane protein VAPA and recruit VAPA to plasma membrane sites, thus
CC linking these intracellular compartments. The ORP3-VAPA complex
CC stimulates RRAS signaling which in turn attenuates integrin beta-1
CC (ITGB1) activation at the cell surface. With VAPA, may regulate ER
CC morphology. Has a role in regulation of the actin cytoskeleton, cell
CC polarity and cell adhesion. Binds to phosphoinositides with preference
CC for PI(3,4)P2 and PI(3,4,5)P3. Also binds 25-hydroxycholesterol and
CC cholesterol. {ECO:0000256|ARBA:ARBA00055107}.
CC -!- SUBUNIT: Homodimer. Interacts with RRAS. Interacts (phosphorylated
CC form) with VAPA. Interacts with OSBPL6.
CC {ECO:0000256|ARBA:ARBA00064163}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|ARBA:ARBA00004202};
CC Peripheral membrane protein {ECO:0000256|ARBA:ARBA00004202}. Cell
CC projection, filopodium tip {ECO:0000256|ARBA:ARBA00004495}. Cytoplasm,
CC cytosol {ECO:0000256|ARBA:ARBA00004514}. Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004406}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004406}. Nucleus membrane
CC {ECO:0000256|ARBA:ARBA00004617}; Peripheral membrane protein
CC {ECO:0000256|ARBA:ARBA00004617}.
CC -!- SIMILARITY: Belongs to the OSBP family. {ECO:0000256|ARBA:ARBA00008842,
CC ECO:0000256|RuleBase:RU003844}.
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DR AlphaFoldDB; A0A3Q1MX43; -.
DR Ensembl; ENSBTAT00000085579.2; ENSBTAP00000060232.2; ENSBTAG00000019342.6.
DR VEuPathDB; HostDB:ENSBTAG00000019342; -.
DR VGNC; VGNC:32462; OSBPL3.
DR GeneTree; ENSGT00940000155957; -.
DR OrthoDB; 1854502at2759; -.
DR Proteomes; UP000009136; Chromosome 4.
DR Bgee; ENSBTAG00000019342; Expressed in oocyte and 100 other cell types or tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008289; F:lipid binding; IEA:UniProtKB-KW.
DR GO; GO:0005319; F:lipid transporter activity; IEA:UniProtKB-ARBA.
DR CDD; cd13287; PH_ORP3_ORP6_ORP7; 1.
DR FunFam; 2.30.29.30:FF:000011; Oxysterol-binding protein; 1.
DR FunFam; 2.40.160.120:FF:000001; Oxysterol-binding protein; 1.
DR FunFam; 3.30.70.3490:FF:000004; Oxysterol-binding protein; 1.
DR Gene3D; 2.40.160.120; -; 1.
DR Gene3D; 3.30.70.3490; -; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR InterPro; IPR037239; OSBP_sf.
DR InterPro; IPR000648; Oxysterol-bd.
DR InterPro; IPR018494; Oxysterol-bd_CS.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR041680; PH_8.
DR InterPro; IPR001849; PH_domain.
DR PANTHER; PTHR10972; OXYSTEROL-BINDING PROTEIN-RELATED; 1.
DR PANTHER; PTHR10972:SF15; OXYSTEROL-BINDING PROTEIN-RELATED PROTEIN 3; 1.
DR Pfam; PF01237; Oxysterol_BP; 1.
DR Pfam; PF15409; PH_8; 1.
DR SMART; SM00233; PH; 1.
DR SUPFAM; SSF144000; Oxysterol-binding protein-like; 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR PROSITE; PS01013; OSBP; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Lipid transport {ECO:0000256|ARBA:ARBA00023055,
KW ECO:0000256|RuleBase:RU003845};
KW Lipid-binding {ECO:0000256|ARBA:ARBA00023121};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000009136};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU003845}.
FT DOMAIN 51..146
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 261..326
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 782..809
FT /evidence="ECO:0000256|SAM:Coils"
FT COMPBIAS 16..32
FT /note="Low complexity"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 268..280
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 851 AA; 97271 MW; 0802A762AA7278BE CRC64;
RMSDEKSLAV SQKLVSPSRS TSSCSSKQGS RQDSWEVVEG LRGEMNYTQE PPIQKGFLLK
KRKWPLKGWH KRFFYLDRGI LKYAKNQTDI EREKLHGCID VGLSVMSVKK SSKCIDLDTE
EHIYHLKVKS EDVFDEWVSK LRHHRMYRQN EIAMFPHEVN HFFPGSTVPD SGPGVMDTIS
SRKRSSISKQ NSFQTGSNLS FSCGGETRVP LWLQSSEDME KCSKDLAHCH AYLVEMSQLL
QNMDILHRTY SAPAINAIQG GAFESPKKEK RSHRRWRSRA LGKDAKGTLQ VPKPFSGPIR
LHSSNPNLST LDFGEEKNYS DGSETSSEFS KMQEDLCHIA HKVYFTLRSA FNTISTEREK
LKQLMEQDVS ASPSAQVLGL KHALSSENSR DDSRALVHQL SNESRLSITD SLSEFFDAQE
VLLSPSSSEN EISEDDSYVS DISDNFSLDN LSNDLDNERQ TLGPVIDSGG EARSKRRTCL
PAPCPKSSNI SLWNILRNNI GKDLSKVAMP VELNEPLNTL QRLCEELEYS ELLDKAAQLP
NALERMVYVA AFAVSAYASS YFRAGSKPFN PVLGETYECI REDKGFRFFS EQVSHHPPIS
ACHAESANFV FWQDVRWKNK FWGKSMEIVP IGTTHVTLPA FGDHFEWNKV TSCIHNILSG
QRWIEHYGEI VIKNLNDDSC HCKVNFIKAK YWSTNAHEIE GTVFDQSGKA VHRLFGKWHE
SIYCGGSSSS ACIWRANPMP KGYEQYYGFT QFALELNEMD PLLKSLLPPT DTRFRPDQRF
LEEGNLEEAE IQKQRIEQLQ RERRRVLEEN KVEHQPRFFR KSIDDSWVSN GTYLELRKDF
GFSKLDHPVL W
//
