ID A0A3Q4G9T2_NEOBR Unreviewed; 374 AA.
AC A0A3Q4G9T2;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 28-JAN-2026, entry version 32.
DE RecName: Full=Deoxyribonuclease-2-alpha {ECO:0000256|ARBA:ARBA00039868};
DE EC=3.1.22.1 {ECO:0000256|ARBA:ARBA00012036};
DE AltName: Full=Acid DNase {ECO:0000256|ARBA:ARBA00041393};
DE AltName: Full=Deoxyribonuclease II alpha {ECO:0000256|ARBA:ARBA00043033};
DE AltName: Full=Lysosomal DNase II {ECO:0000256|ARBA:ARBA00041918};
OS Neolamprologus brichardi (Fairy cichlid) (Lamprologus brichardi).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Ovalentaria; Cichlomorphae; Cichliformes; Cichlidae; African cichlids;
OC Pseudocrenilabrinae; Lamprologini; Neolamprologus.
OX NCBI_TaxID=32507 {ECO:0000313|Ensembl:ENSNBRP00000005231.1, ECO:0000313|Proteomes:UP000261580};
RN [1] {ECO:0000313|Ensembl:ENSNBRP00000005231.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (AUG-2025) to UniProtKB.
RN [2] {ECO:0000313|Ensembl:ENSNBRP00000005231.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (SEP-2025) to UniProtKB.
CC -!- FUNCTION: Hydrolyzes DNA under acidic conditions with a preference for
CC double-stranded DNA. Plays a major role in the clearance of nucleic
CC acids generated through apoptosis, hence preventing autoinflammation.
CC Necessary for proper fetal development and for definitive
CC erythropoiesis in fetal liver and bone marrow, where it degrades
CC nuclear DNA expelled from erythroid precursor cells.
CC {ECO:0000256|ARBA:ARBA00045381}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-
CC phosphooligonucleotide end-products.; EC=3.1.22.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000447};
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000256|ARBA:ARBA00004371}.
CC -!- SIMILARITY: Belongs to the DNase II family.
CC {ECO:0000256|ARBA:ARBA00007527}.
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DR RefSeq; XP_006801855.1; XM_006801792.2.
DR AlphaFoldDB; A0A3Q4G9T2; -.
DR STRING; 32507.ENSNBRP00000005231; -.
DR Ensembl; ENSNBRT00000005388.1; ENSNBRP00000005231.1; ENSNBRG00000004163.1.
DR GeneID; 102785627; -.
DR CTD; 1777; -.
DR GeneTree; ENSGT00390000002634; -.
DR OMA; YLMYNDE; -.
DR OrthoDB; 10261598at2759; -.
DR Proteomes; UP000261580; Unassembled WGS sequence.
DR Bgee; ENSNBRG00000004163; Expressed in mesonephros and 4 other cell types or tissues.
DR GO; GO:0005764; C:lysosome; IEA:UniProtKB-SubCell.
DR GO; GO:0004531; F:deoxyribonuclease II activity; IEA:UniProtKB-EC.
DR GO; GO:0006309; P:apoptotic DNA fragmentation; IEA:TreeGrafter.
DR InterPro; IPR004947; DNase_II.
DR PANTHER; PTHR10858; DEOXYRIBONUCLEASE II; 1.
DR PANTHER; PTHR10858:SF9; DEOXYRIBONUCLEASE-2-ALPHA; 1.
DR Pfam; PF03265; DNase_II; 1.
PE 3: Inferred from homology;
KW Apoptosis {ECO:0000256|ARBA:ARBA00022703};
KW Developmental protein {ECO:0000256|ARBA:ARBA00022473};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Endonuclease {ECO:0000256|ARBA:ARBA00022759};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lysosome {ECO:0000256|ARBA:ARBA00023228};
KW Nuclease {ECO:0000256|ARBA:ARBA00022722};
KW Reference proteome {ECO:0000313|Proteomes:UP000261580};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..18
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 19..374
FT /note="Deoxyribonuclease-2-alpha"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5018715149"
SQ SEQUENCE 374 AA; 41376 MW; 53BAD9C770DCBF3D CRC64;
MLLFLSVLIL YVPLEGSSSP IGCYNDQGDA VDWFYIYKLP KEHGSKSPER GEMYLLLDQG
SEGWTEGKGT VNDTTSALGR TVGQLYTQGK NTDIAYILYN DQKPHEDSSG DRWVHNSESS
GGHTKGVVLL DKNQGFWLVH STPQFPPVQQ AGEYYYPSSG ENNGQNFICV TYPLDHFQTI
GEQLEINQPK VYDCSIPESL ASQVPSLAAL CGKRNRKYEF FPHAKPVSNR SVTLTSKGGT
NFISFAKGAS FDNDLYHAWV APTLQSDLLV QFWIRSTGIL PSDCSDDWKV LNIKLLNPGQ
TFSFKTSQDH SKWAVSPSET GAGGGWVCVG DINRDEAEEK RGGGTVCHRD PVVWKAYRTA
ALDCEDCQGE TVQC
//
