ID A0A3Q7K726_CICAR Unreviewed; 323 AA.
AC A0A3Q7K726;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 10-JUN-2026, entry version 33.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN Name=LOC101510489 {ECO:0000313|RefSeq:NP_001351658.1};
OS Cicer arietinum (Chickpea) (Garbanzo).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Cicereae; Cicer.
OX NCBI_TaxID=3827 {ECO:0000313|Proteomes:UP000087171, ECO:0000313|RefSeq:NP_001351658.1};
RN [1] {ECO:0000313|RefSeq:NP_001351658.1}
RP NUCLEOTIDE SEQUENCE.
RX PubMed=19912666;
RA Varshney R.K., Hiremath P.J., Lekha P., Kashiwagi J., Balaji J.,
RA Deokar A.A., Vadez V., Xiao Y., Srinivasan R., Gaur P.M., Siddique K.H.,
RA Town C.D., Hoisington D.A.;
RT "A comprehensive resource of drought- and salinity- responsive ESTs for
RT gene discovery and marker development in chickpea (Cicer arietinum L.).";
RL BMC Genomics 10:523-523(2009).
RN [2] {ECO:0000313|Proteomes:UP000087171}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. CDC Frontier {ECO:0000313|Proteomes:UP000087171};
RX PubMed=23354103;
RA Varshney R.K., Song C., Saxena R.K., Azam S., Yu S., Sharpe A.G.,
RA Cannon S., Baek J., Rosen B.D., Tar'an B., Millan T., Zhang X.,
RA Ramsay L.D., Iwata A., Wang Y., Nelson W., Farmer A.D., Gaur P.M.,
RA Soderlund C., Penmetsa R.V., Xu C., Bharti A.K., He W., Winter P., Zhao S.,
RA Hane J.K., Carrasquilla-Garcia N., Condie J.A., Upadhyaya H.D., Luo M.C.,
RA Thudi M., Gowda C.L., Singh N.P., Lichtenzveig J., Gali K.K., Rubio J.,
RA Nadarajan N., Dolezel J., Bansal K.C., Xu X., Edwards D., Zhang G.,
RA Kahl G., Gil J., Singh K.B., Datta S.K., Jackson S.A., Wang J., Cook D.R.;
RT "Draft genome sequence of chickpea (Cicer arietinum) provides a resource
RT for trait improvement.";
RL Nat. Biotechnol. 31:240-246(2013).
RN [3] {ECO:0000313|RefSeq:NP_001351658.1}
RP IDENTIFICATION.
RG RefSeq;
RL Submitted (JAN-2026) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC {ECO:0000256|ARBA:ARBA00004906}.
CC -!- SIMILARITY: Belongs to the SINA (Seven in absentia) family.
CC {ECO:0000256|ARBA:ARBA00009119}.
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DR RefSeq; NP_001351658.1; NM_001364729.1.
DR AlphaFoldDB; A0A3Q7K726; -.
DR STRING; 3827.A0A3Q7K726; -.
DR PaxDb; 3827-A0A3Q7K726; -.
DR GeneID; 101510489; -.
DR KEGG; cam:101510489; -.
DR OrthoDB; 941555at2759; -.
DR UniPathway; UPA00143; -.
DR Proteomes; UP000087171; Chromosome Ca5.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
DR CDD; cd16571; RING-HC_SIAHs; 1.
DR CDD; cd03829; Sina; 1.
DR FunFam; 3.30.40.10:FF:000041; E3 ubiquitin-protein ligase SINAT3; 1.
DR FunFam; 3.30.40.10:FF:000483; E3 ubiquitin-protein ligase SINAT3; 1.
DR FunFam; 2.60.210.10:FF:000004; E3 ubiquitin-protein ligase SINAT5-like; 1.
DR Gene3D; 2.60.210.10; Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2, Chain A; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 2.
DR InterPro; IPR018121; 7-in-absentia-prot_TRAF-dom.
DR InterPro; IPR052088; E3_ubiquitin-ligase_SINA.
DR InterPro; IPR049548; Sina-like_RING.
DR InterPro; IPR008974; TRAF-like.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR013010; Znf_SIAH.
DR PANTHER; PTHR10315; E3 UBIQUITIN PROTEIN LIGASE SIAH; 1.
DR PANTHER; PTHR10315:SF80; E3 UBIQUITIN-PROTEIN LIGASE SINAT3; 1.
DR Pfam; PF21362; Sina_RING; 1.
DR Pfam; PF03145; Sina_TRAF; 1.
DR Pfam; PF21361; Sina_ZnF; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR SUPFAM; SSF49599; TRAF domain-like; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
DR PROSITE; PS51081; ZF_SIAH; 1.
PE 3: Inferred from homology;
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000087171};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00455}.
FT DOMAIN 61..97
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
FT DOMAIN 114..174
FT /note="SIAH-type"
FT /evidence="ECO:0000259|PROSITE:PS51081"
FT REGION 1..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 323 AA; 36773 MW; 503DD1BA33B5BEFB CRC64;
MELDNIECVS SSDGMDEDEI HQHHHSEFSS TKHRNGANNN NNILGPTAIA PATSVHELLE
CPVCTNSMYP PIHQCHNGHT LCSTCKTRVH NRCPTCRQEL GDIRCLALEK VAESLELPCK
YYSLGCPEIF PYYSKLKHET VCNFRPYSCP YAGSECSVVG DISFLVTHLR DDHKVDMHTG
CTFNHRYVKS NPREVENATW MLTVFHCFGQ YFCLHFEAFQ LGMAPVYMAF LRFMGDENEA
RNYSYSLEVG GNGRKLIWEG TPRSVRDSHR KVRDSHDGLI IQRNMALFFS GGDRKELKLR
VTGRIWKEQQ NPDAGVCIPN LCS
//
