ID A0A3S2W5U9_9BACI Unreviewed; 719 AA.
AC A0A3S2W5U9;
DT 10-APR-2019, integrated into UniProtKB/TrEMBL.
DT 10-APR-2019, sequence version 1.
DT 18-JUN-2025, entry version 19.
DE RecName: Full=DNA topoisomerase {ECO:0000256|ARBA:ARBA00012891};
DE EC=5.6.2.1 {ECO:0000256|ARBA:ARBA00012891};
DE AltName: Full=Omega-protein {ECO:0000256|ARBA:ARBA00032877};
DE AltName: Full=Relaxing enzyme {ECO:0000256|ARBA:ARBA00032235};
DE AltName: Full=Swivelase {ECO:0000256|ARBA:ARBA00030003};
DE AltName: Full=Untwisting enzyme {ECO:0000256|ARBA:ARBA00031985};
GN ORFNames=EM808_08135 {ECO:0000313|EMBL:RVT65457.1};
OS Niallia taxi.
OC Bacteria; Bacillati; Bacillota; Bacilli; Bacillales; Bacillaceae; Niallia.
OX NCBI_TaxID=2499688 {ECO:0000313|EMBL:RVT65457.1, ECO:0000313|Proteomes:UP000288024};
RN [1] {ECO:0000313|EMBL:RVT65457.1, ECO:0000313|Proteomes:UP000288024}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M5HDSG1-1 {ECO:0000313|EMBL:RVT65457.1,
RC ECO:0000313|Proteomes:UP000288024};
RA Tuo L.;
RT "Bacillus sp. M5HDSG1-1, whole genome shotgun sequence.";
RL Submitted (JAN-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-independent breakage of single-stranded DNA, followed by
CC passage and rejoining.; EC=5.6.2.1;
CC Evidence={ECO:0000256|ARBA:ARBA00000213};
CC -!- SIMILARITY: Belongs to the type IA topoisomerase family.
CC {ECO:0000256|ARBA:ARBA00009446}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:RVT65457.1}.
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DR EMBL; RZTZ01000002; RVT65457.1; -; Genomic_DNA.
DR RefSeq; WP_127737674.1; NZ_CAJCKN010000093.1.
DR AlphaFoldDB; A0A3S2W5U9; -.
DR GeneID; 87616511; -.
DR Proteomes; UP000288024; Unassembled WGS sequence.
DR GO; GO:0043597; C:cytoplasmic replication fork; IEA:TreeGrafter.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003917; F:DNA topoisomerase type I (single strand cut, ATP-independent) activity; IEA:UniProtKB-EC.
DR GO; GO:0006310; P:DNA recombination; IEA:TreeGrafter.
DR GO; GO:0006281; P:DNA repair; IEA:TreeGrafter.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd00186; TOP1Ac; 1.
DR CDD; cd03362; TOPRIM_TopoIA_TopoIII; 1.
DR Gene3D; 3.40.50.140; -; 1.
DR Gene3D; 1.10.460.10; Topoisomerase I, domain 2; 1.
DR Gene3D; 2.70.20.10; Topoisomerase I, domain 3; 1.
DR Gene3D; 1.10.290.10; Topoisomerase I, domain 4; 1.
DR InterPro; IPR000380; Topo_IA.
DR InterPro; IPR003601; Topo_IA_2.
DR InterPro; IPR013497; Topo_IA_cen.
DR InterPro; IPR013824; Topo_IA_cen_sub1.
DR InterPro; IPR013825; Topo_IA_cen_sub2.
DR InterPro; IPR013826; Topo_IA_cen_sub3.
DR InterPro; IPR023405; Topo_IA_core_domain.
DR InterPro; IPR003602; Topo_IA_DNA-bd_dom.
DR InterPro; IPR025589; Toprim_C_rpt.
DR InterPro; IPR006171; TOPRIM_dom.
DR InterPro; IPR034144; TOPRIM_TopoIII.
DR PANTHER; PTHR11390:SF21; DNA TOPOISOMERASE 3-ALPHA; 1.
DR PANTHER; PTHR11390; PROKARYOTIC DNA TOPOISOMERASE; 1.
DR Pfam; PF01131; Topoisom_bac; 1.
DR Pfam; PF01751; Toprim; 1.
DR Pfam; PF13342; Toprim_Crpt; 1.
DR PRINTS; PR00417; PRTPISMRASEI.
DR SMART; SM00437; TOP1Ac; 1.
DR SMART; SM00436; TOP1Bc; 1.
DR SMART; SM00493; TOPRIM; 1.
DR SUPFAM; SSF56712; Prokaryotic type I DNA topoisomerase; 1.
DR PROSITE; PS52039; TOPO_IA_2; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:RVT65457.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000288024};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 1..141
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
FT DOMAIN 161..599
FT /note="Topo IA-type catalytic"
FT /evidence="ECO:0000259|PROSITE:PS52039"
SQ SEQUENCE 719 AA; 81972 MW; 727C6AD6C507173A CRC64;
MKLILAEKPS VSKNIADALK IKNKQDGYFE GNGYIITWAF GHLLQLNDAK DYDEKMATWK
MDNFPFIPES FKYKVKSDPR NREKPDKGAQ KQLRTIQQLM RRQDVTAIIS ACDYDREGQI
IGDSIIYNLR TDKEVYRLLL NEWTPDEVLN GLEQIRANSE MRPLQDAGVS RQWADWLIGI
NLTSVTTLKY QKGKGKALNI GRVLLPTLKI IYDRDKEIRD FVPEDYFKLQ GTFSSTEGKY
TGIYTVGKED KFKTKEELDQ LQKQIAGKKG IVADKTVQKK KEYPPLLFNL SNLQGYITSK
YKGWTADKVL KVAQGLYEKK YITYPRTASM ALEESLIGKT AKVVSAISAN LPYKDDIKFY
ASKRVFDNKK VESHSAIIPT YLIPKRLTND EEQVYEAIKT RLLMQFMPVA EYEETRIVTE
IKDTSLPGAF HTKGKVQLVE GWKKAENIKT KDVLLPYVGI GEEVDVKKIE TTTHTTQAPK
EHTEKTLLRL METCGKNIDA LSDTEEDVMS ILSGFSIGTP ATRADTIKKL KDIGYIEAKG
KSLICTDLGK TLVETFPIKE LFNLDFTGRL EKSLYDIEKG NYSKQAFLQL ISEFTKNSVE
KIKHEQDVTI QEVSYTKRKI ENLGKCPVCG HQVIEGQKGF GCSNWKQGCK FVIWKNDKFL
AALKKKPTKT MVKAILKNGS AEVKGLTSKK GNKFDAILKY VKNTDNDYFS WQMDFASKK
//
