ID A0A4D9DVV7_9SAUR Unreviewed; 487 AA.
AC A0A4D9DVV7;
DT 03-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 03-JUL-2019, sequence version 1.
DT 08-OCT-2025, entry version 15.
DE RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Name=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN Synonyms=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN ORFNames=DR999_PMT19019 {ECO:0000313|EMBL:TFJ99002.1};
OS Platysternon megacephalum (big-headed turtle).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Testudinata; Testudines; Cryptodira; Durocryptodira;
OC Testudinoidea; Platysternidae; Platysternon.
OX NCBI_TaxID=55544 {ECO:0000313|EMBL:TFJ99002.1, ECO:0000313|Proteomes:UP000297703};
RN [1] {ECO:0000313|EMBL:TFJ99002.1, ECO:0000313|Proteomes:UP000297703}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DO16091913 {ECO:0000313|EMBL:TFJ99002.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:TFJ99002.1};
RA Gong S.;
RT "Draft genome of the big-headed turtle Platysternon megacephalum.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:TFJ99002.1, ECO:0000313|Proteomes:UP000297703}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DO16091913 {ECO:0000313|EMBL:TFJ99002.1};
RC TISSUE=Muscle {ECO:0000313|EMBL:TFJ99002.1};
RA Gong S.;
RT "The genome sequence of big-headed turtle.";
RL Submitted (APR-2019) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from
CC thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC Rule:MF_03054}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TFJ99002.1}.
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DR EMBL; QXTE01000355; TFJ99002.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4D9DVV7; -.
DR STRING; 55544.A0A4D9DVV7; -.
DR OrthoDB; 25129at2759; -.
DR UniPathway; UPA00988; -.
DR Proteomes; UP000297703; Unassembled WGS sequence.
DR GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR HAMAP; MF_03054; CTU2; 1.
DR InterPro; IPR019407; CTU2.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR Pfam; PF10288; CTU2; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 3: Inferred from homology;
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW Reference proteome {ECO:0000313|Proteomes:UP000297703};
KW tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW Rule:MF_03054}.
SQ SEQUENCE 487 AA; 53649 MW; 27E53CF6055D611F CRC64;
MKCKEASPVL VIRVGDAFCK ACFREYFVHK FRAMLGKNRA IYPGEKVLLA LSGGPASSSM
LRQVQEGLSR ETAKKLRFIP GIIYVDEGAV CGQSLAEREE TLLQMETILQ ASGFPYHLAH
LEEVFDLPSS ILQRVPHNPA GPKNSYKEAV EGFIRQQRQE EGGGPSLPER QIQEKLAEIS
MRDAPGMEGL AAPEARLLAA VRTEQLIRLF GSVKTLTAKE ELLQTLRNHL ILHTARAQGY
SKAMMGDSCT RLAVKLLTNL SLGRGASLAM DTGFSDDRHG DVVVVRPMRE YSAKEIAFYN
HLFGVPTVFT PALDTKALEK ASIHRVIERF LYRLQAEFPS TVSTVYRTGE KLSATPGDAS
PAAEPERCLL CLCALDTNVE DGSALQAILV SEQLSQQKLP AALPTERGCC PGAEEQRGCC
MNTTAPGADF LPLLCYGCRL TIKDTTCLES LPLYVRAEAE RRKRRAAMKL EIQEFLLEDE
AVTPDES
//
