ID A0A4S2JEN5_9HYME Unreviewed; 4090 AA.
AC A0A4S2JEN5;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 18-JUN-2025, entry version 20.
DE RecName: Full=Pre-mRNA-processing-splicing factor 8 {ECO:0000256|ARBA:ARBA00071757};
DE AltName: Full=Splicing factor Prp8 {ECO:0000256|ARBA:ARBA00079260};
GN ORFNames=DBV15_00998 {ECO:0000313|EMBL:TGZ32787.1};
OS Temnothorax longispinosus.
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Hymenoptera; Apocrita; Aculeata; Formicoidea;
OC Formicidae; Myrmicinae; Temnothorax.
OX NCBI_TaxID=300112 {ECO:0000313|EMBL:TGZ32787.1, ECO:0000313|Proteomes:UP000310200};
RN [1] {ECO:0000313|EMBL:TGZ32787.1, ECO:0000313|Proteomes:UP000310200}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC TISSUE=Whole body {ECO:0000313|EMBL:TGZ32787.1};
RX PubMed=30967075;
RA Kaur R., Stoldt M., Jongepier E., Feldmeyer B., Menzel F.,
RA Bornberg-Bauer E., Foitzik S.;
RT "Ant behaviour and brain gene expression of defending hosts depend on the
RT ecological success of the intruding social parasite.";
RL Philos. Trans. R. Soc. Lond., B, Biol. Sci. 374:20180192-20180192(2019).
CC -!- FUNCTION: Plays a role in pre-mRNA splicing as core component of
CC precatalytic, catalytic and postcatalytic spliceosomal complexes, both
CC of the predominant U2-type spliceosome and the minor U12-type
CC spliceosome. Functions as a scaffold that mediates the ordered assembly
CC of spliceosomal proteins and snRNAs. Required for the assembly of the
CC U4/U6-U5 tri-snRNP complex, a building block of the spliceosome.
CC Functions as a scaffold that positions spliceosomal U2, U5 and U6
CC snRNAs at splice sites on pre-mRNA substrates, so that splicing can
CC occur. Interacts with both the 5' and the 3' splice site.
CC {ECO:0000256|ARBA:ARBA00055739}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TGZ32787.1}.
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DR EMBL; QBLH01003812; TGZ32787.1; -; Genomic_DNA.
DR STRING; 300112.A0A4S2JEN5; -.
DR Proteomes; UP000310200; Unassembled WGS sequence.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IEA:TreeGrafter.
DR GO; GO:0005682; C:U5 snRNP; IEA:TreeGrafter.
DR GO; GO:0008237; F:metallopeptidase activity; IEA:InterPro.
DR GO; GO:0097157; F:pre-mRNA intronic binding; IEA:TreeGrafter.
DR GO; GO:0030619; F:U1 snRNA binding; IEA:TreeGrafter.
DR GO; GO:0030620; F:U2 snRNA binding; IEA:TreeGrafter.
DR GO; GO:0030623; F:U5 snRNA binding; IEA:InterPro.
DR GO; GO:0017070; F:U6 snRNA binding; IEA:InterPro.
DR GO; GO:0000244; P:spliceosomal tri-snRNP complex assembly; IEA:TreeGrafter.
DR CDD; cd08056; MPN_PRP8; 2.
DR CDD; cd13838; RNase_H_like_Prp8_IV; 1.
DR FunFam; 1.20.80.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.30.420.230:FF:000003; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.30.43.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR FunFam; 3.40.140.10:FF:000002; Pre-mRNA-processing-splicing factor 8; 2.
DR FunFam; 3.90.1570.40:FF:000001; Pre-mRNA-processing-splicing factor 8; 1.
DR Gene3D; 1.20.80.40; -; 2.
DR Gene3D; 3.30.420.230; -; 1.
DR Gene3D; 3.90.1570.40; -; 1.
DR Gene3D; 3.40.140.10; Cytidine Deaminase, domain 2; 2.
DR Gene3D; 3.30.43.40; Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding domain; 1.
DR InterPro; IPR000555; JAMM/MPN+_dom.
DR InterPro; IPR037518; MPN.
DR InterPro; IPR012591; PRO8NT.
DR InterPro; IPR012592; PROCN.
DR InterPro; IPR012984; PROCT.
DR InterPro; IPR027652; PRP8.
DR InterPro; IPR021983; PRP8_domainIV.
DR InterPro; IPR043173; Prp8_domainIV_fingers.
DR InterPro; IPR043172; Prp8_domainIV_palm.
DR InterPro; IPR019581; Prp8_U5-snRNA-bd.
DR InterPro; IPR042516; Prp8_U5-snRNA-bd_sf.
DR InterPro; IPR019580; Prp8_U6-snRNA-bd.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR019582; RRM_spliceosomal_PrP8.
DR PANTHER; PTHR11140; PRE-MRNA SPLICING FACTOR PRP8; 1.
DR PANTHER; PTHR11140:SF0; PRE-MRNA-PROCESSING-SPLICING FACTOR 8; 1.
DR Pfam; PF01398; JAB; 2.
DR Pfam; PF08082; PRO8NT; 1.
DR Pfam; PF08083; PROCN; 1.
DR Pfam; PF08084; PROCT; 2.
DR Pfam; PF12134; PRP8_domainIV; 1.
DR Pfam; PF10598; RRM_4; 2.
DR Pfam; PF10597; U5_2-snRNA_bdg; 1.
DR Pfam; PF10596; U6-snRNA_bdg; 1.
DR SMART; SM00232; JAB_MPN; 2.
DR SUPFAM; SSF53098; Ribonuclease H-like; 4.
DR PROSITE; PS50249; MPN; 2.
PE 4: Predicted;
KW mRNA processing {ECO:0000256|ARBA:ARBA00022664};
KW mRNA splicing {ECO:0000256|ARBA:ARBA00023187};
KW Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000310200};
KW Ribonucleoprotein {ECO:0000256|ARBA:ARBA00023274};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884};
KW Spliceosome {ECO:0000256|ARBA:ARBA00022728}.
FT DOMAIN 2924..3055
FT /note="MPN"
FT /evidence="ECO:0000259|PROSITE:PS50249"
FT DOMAIN 3328..3451
FT /note="MPN"
FT /evidence="ECO:0000259|PROSITE:PS50249"
FT REGION 114..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 135..164
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..229
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4090 AA; 473555 MW; 6FDE1399524FA0DA CRC64;
MSNFYKTDNF DDSELQARLY ERIYYDFSIQ TDSDIEGDSR ITDAPRTIQL DSMITETNVK
SDRLQHEFEE QPLASKTAGE QLICNNTYIR CSADSQTMPS TEFTQVHTLA DREMNPSAHG
CDNESSEDLP VTQGKENKEN SSKNDKSSKY AKNEHQESSE KDHNNTAYSK YNVVSKCEEC
GECVAKQTDK DEDNSDXKYN VVSKCEECGX CVAKQTDKDE DNSDSEESIC EVPIPPKPEP
PLIDLQDSDA RTEDVEPSAN IDTLSSRINF YILPKEVVDA VVKNIWAVRT KPTQTKVYNV
TFIEIIYSYG YFRNKNHQDA RMILKNLNNI KPFKQLNSLI NRKVAAPVFL NTLLEKPIEF
EVKIGFTDRR NLQLHLLGMK EYIEELYELL RYWLNLRDDE KDYGIDVTTP PINPIKMYNL
LNSRLLQLEK MRFTFNAEHI GGMNDPQWIY SCINDEKIKL VQHEKNTKIY NQSRKKLLQL
QTRLLMIANT ESKSNNRSNS LVHMFQRMMR EFESNRHLMG KRLDTSTYLR LILLYNQLFV
PHTSVSVQQM LRRIRLEEKR KNKKNSQQMQ QEITVPDQGN VVYNVYSSPT ASCVPQNINF
DNYSAINQQN NEMLVEGQNI NNIPLETIKP TDRNCDEITI YSPTQDSKII MPLNIEPVSL
KDYERNESQL STSEVKIEPN LFENKRNINV NNMVHIPCLT KKYEKRLKEL KIKTTVDLIR
KARAFKVPHM MNVADEMERR IRNQTITPKH IKTLSKLVLI EEKYQKEVNV CCKKLQNWET
PAIMSIPPYL LPPGPWATMM AAQQQAQIAA QVHAQAAAHA QAAHHAHMQA HIAGPPLPQM
PKQPEVLSED KLQEKAQKWQ QLQSKRFAEK RKFGFVDAQK EDMPAEHIRK IIRDHGDMSS
RKYRHDKRVY LGALKYMPHA VMKLLENMPM PWEQIRDVKV LYHITGAITF VNEIPWVIEP
VYIAQWGTMW IMMRREKRDR RHFKRMRFPP FDDEEPPLDY ADNVLDVEPL EAIQIELDSE
EDESVASWFY EHKPLVGTKH VNGSTYRRWN LTLPQMATLY RLANQLLTDL VDQNFFYLFD
PKSFFTAKAL NMAIPGGPKF EPLVKDSNAA DEDWNEFNDI NKIIIRQPIR TEYRIAFPYL
YNNMPHFVHL SWYHAPNVVY IKTEDPDLPA FYFDPLINPI SHRNSLKTVE PQIEDDEDFV
LPGEVQPFLQ ETPLYTDNTA NGIALLWAPR PFNTRSGRTR RAIDIPLVKS WYREHCPPGQ
PVKVRVSYQK LLKYFVLNAL KHRPPKPQKK RYLFRSFKST KFFQTTTIDW VEAGLQVCRQ
GYNMLNLLIH RKNLNYLHLD YNFNLKPVKT LTTKERKKSR FGNAFHLCRE ILRLTKLIID
SHVQYRLNNV DAFQLADGLQ YIFAHVGQLT GMYRYKYKLM RQIRMCKDLK HLIYYRFNTG
PVGKGPGCGF WAPGWRVWLF FMRGITPLLE RWLGNLLSRQ FEGRHSKGVA KTVTKQRVES
HFDLELRASV MHDIVDMMPE GIKQNKARTI LQHLSEAWRC WKANIPWKVP GLPIPIENMI
LRYVKMKADW WTNTAHYNRE RIRRGATVDK TVCKKNLGRL TRLYLKAEQE RQHNYLKDGP
YISPEEAVAI FTTSVHWLES RRFAPIPFPP LSYKHDTKLL ILALERLKEA YSVKSRLNQS
QREELGLIEQ AYDNPHEALS RIKRHLLTQR AFKEVGIEFM DLYSHLIPVY DVEPLEKITD
AYLDQYLWYE ADKRRLFPPW VKPADTEPPP LLVYKWCQGI NNLQDVWDVG EGECNVLLES
KFEKLYEKID LTLLNRLLRL IVDHNIADYM TAKNNVVINY KDMNHTNSYG IIRGLQFASF
IAQYYGLVLD LLVLGLQRAS EMAGPPQMPN DFLTFQDVAS ETAHPIRLYC RYVDRIHLFL
RFSADEARDL IQRYLTEHPD PNNENIVGYN NKKCWPRDAR MRLMKHDVNL GRAVFWDIKN
RLPRSTTTIQ WENSFVSVYS KDNPNLLFNM SGFECRILPK CRTTHEEFTH RDGVWNLQNE
ITKERTAQCF LRVDDESLQR FHNRVRQILM ASGSTTFTKI VNKWNTALIG LMTYFREAVV
NTQELLDLLV KCENKIQTRI KIGLNSKMPS RFPPVVFYTP KELGGLGMLS MGHVLIPQSD
LRWSKQTDVG ITHFRSGMSH DEDQLIPNLY RYIQPWESEF IDSQRVWAEY ALKRQEANAQ
NRRLTLEDLE DSWDRGIPRI NTLFQKDRHT LAYDKGWRIR TEFKQYQVLK QNPFWWTHQR
HDGKLWNLNN YRTDMIQALG GVEGILEHTL FKGTYFPTWE GLFWEKASGF EESMKYKKLT
NAQRSGLNQI PNRRFTLWWS PTINRANVYV GFQVQLDLTG IFMHGKIPTL KISLIQIFRA
HLWQKVHESI VMDLCQVFDQ ELDALEIETV QKETIHPRKS YKMNSSCADI LLFSAYKWNV
SRPSLLADSK DVMDNTTTQK YWIDVQLRWG DYDSHDIERY ARAKFLDYTT DNMSIYPSPT
GLLIAIDLAY NLHSAYGNWF PGCKPLIQQA MAKIMKANPA LYVLRERIRK ALQLYSSEPT
EPYLSSQNYG ELFSNQIIWF VDDTNVYRVT IHKTFEGNLT TKPINGAIFI FNPRTGQLFL
KIIHTSVWAG QKRLGQLAKW KTAEEVAALI RSLPVEEQPK QIIVTRKGML DPLEVHLLDF
PNIVIKGSEL QLPFQACLKV EKFGDLILKA TEPQMVLFNL YDDWLKTISS YTAFSRLILI
LRALHVNTER TKVVLKPDKT TITEPHHIWP SLTDDEWIKV EVQLKDLILA DYGKKNNVNV
ASLTQSEIRD IILGMEISAP SAQRQQIAEI EKQTKEQSQL TATTTRTVNK HGDEIITATT
SNYETQTFSS KTEWRVRAIS ATNLHLRTNH IYVSSDDIKE TGYTYILPKN VLKKFVTISD
LRAQIAGYLY GISPPDNPQV KEIRCIVMAP QWGTHQTVHL PNTLPNHQYL KEMEPLGWIH
TQPNELPQLS PQDISTHARI MAENTIWDGE KTIVITCSFT PGSCSLTAYK LTPSGFEWGK
QNTDKGNNPK GYLPSHYEKV QMLLSDRFLG FXMVPSQGSW NYNFMGVRHD PNMKYELQLA
NPKEFYHEVH RPSHFLNFSS LEDGDGXGAD REDICPATAT ISQTTERRKI RVTISKTTET
KRLGNGRDGV TAQYDGRKPA HIHAKQYLKD LILADYGKKN NVNVASLTQS EIRDIILNME
LSASSAQRQQ IAEIEKQTKE QSQLTATTTR TVNKHGDEII TATTSNYETQ TFSSKTEWRV
GAISATNLHL RTNHIYVSSD DIKETGYTYI LPKNVLKEFV TISDLRVQIA GYLYGISPPD
YPQMYCNGAA MGNSSDRSLT KHATQPSEME PLGWIHTQPN ELPQLSPQDI STHARIMAEN
TIWDGEKTIV ITCSFTPGSC SLTAYKLTPS GFEWGKQNTD KGNNPKGYLP SHYEKVQVLL
SDRFLGFFMV PSQGSWNYNF MGERHDPNMK YELQLENPKE FYHEPTRMST KCQSTSRLTR
SSLPKPRKVC ATRFSAPVER SATVSGGLRK LRIDLRTIGQ RARYLSGEHT LLHNAYCCSG
GEKRYSFGKT AETEDRSTDD RTTSEQQKFQ VAPGGVPLTC GEAQHTYRRH VNVVDQDQED
VGDDEADDIA HERLAVEIPQ LRGNRVHQEG GDEERDGDHD ADMVVNLVDV LNLLAHLVPR
QTRIMPGCRA CQLHRIVLGH LLAYRFLLRI PENLVVAAAF LDAVAQFANA TMTSLENINN
ILKTADAVEF DVDSITSSPI LALEHLKEAI SVKSRLNQSQ REELGLITQA YDNPHEALSR
IKRHLFTQRA FKEVNIKFTD LYSHLIPDVE PLEKITDAYL DQYLWYEANK KRICDKKRMF
PPWTKPADTE PPPLLVYKWC QGINNLADVW DVSEVDHNIA DYMTAKNNVV INYKDMNHTN
SYGIIRGLQF ASFIAQYYGL VLDLLVLGLQ RASEMAGPPQ MPNDFLTFQD VASETAHPIR
LYCRYVDRIY LFLRFSADDA RDLIQRYLTE HPDPNNKNIV GYNNKSAHFL NFSSLEDGDG
VGADXEDLFA
//
