ID A0A4S2M1C0_OPIFE Unreviewed; 410 AA.
AC A0A4S2M1C0;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 02-APR-2025, entry version 15.
DE RecName: Full=RBR-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012251};
DE EC=2.3.2.31 {ECO:0000256|ARBA:ARBA00012251};
GN ORFNames=CRM22_003414 {ECO:0000313|EMBL:TGZ70011.1};
OS Opisthorchis felineus.
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Platyhelminthes; Trematoda;
OC Digenea; Opisthorchiida; Opisthorchiata; Opisthorchiidae; Opisthorchis.
OX NCBI_TaxID=147828 {ECO:0000313|EMBL:TGZ70011.1, ECO:0000313|Proteomes:UP000308267};
RN [1] {ECO:0000313|EMBL:TGZ70011.1, ECO:0000313|Proteomes:UP000308267}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AK-0245 {ECO:0000313|EMBL:TGZ70011.1};
RC TISSUE=Whole organism {ECO:0000313|EMBL:TGZ70011.1};
RX PubMed=31117933; DOI=10.1186/s12864-019-5752-8;
RA Ershov N.I., Mordvinov V.A., Prokhortchouk E.B., Pakharukova M.Y.,
RA Gunbin K.V., Ustyantsev K., Genaev M.A., Blinov A.G., Mazur A.,
RA Boulygina E., Tsygankova S., Khrameeva E., Chekanov N., Fan G., Xiao A.,
RA Zhang H., Xu X., Yang H., Solovyev V., Lee S.M., Liu X., Afonnikov D.A.,
RA Skryabin K.G.;
RT "New insights from Opisthorchis felineus genome: update on genomics of the
RT epidemiologically important liver flukes.";
RL BMC Genomics 20:399-399(2019).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + [acceptor protein]-N(6)-ubiquitinyl-L-lysine.;
CC EC=2.3.2.31; Evidence={ECO:0000256|ARBA:ARBA00001798};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:TGZ70011.1}.
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DR EMBL; SJOL01005652; TGZ70011.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4S2M1C0; -.
DR OrthoDB; 1431934at2759; -.
DR Proteomes; UP000308267; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd20338; BRcat_RBR_RNF19; 1.
DR Gene3D; 1.20.120.1750; -; 1.
DR InterPro; IPR031127; E3_UB_ligase_RBR.
DR InterPro; IPR002867; IBR_dom.
DR InterPro; IPR044066; TRIAD_supradom.
DR PANTHER; PTHR11685; RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING; 1.
DR Pfam; PF01485; IBR; 2.
DR SMART; SM00647; IBR; 2.
DR SUPFAM; SSF57850; RING/U-box; 2.
DR PROSITE; PS51873; TRIAD; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000308267};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT TRANSMEM 332..361
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 381..403
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1..322
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS51873"
SQ SEQUENCE 410 AA; 45769 MW; 149E4B4C90DCA4F5 CRC64;
MPGCCHMACV PCWVTFICTE IENFAMSRLT CIGCKKALST STVLRVLTIP IGNSAAFGLC
MNNPLQLTTI QTHCLKMLSR YEEFLLHSAV SDEPDFRWCP NGCGYGVIAH GFQRCPQLYC
QRPECNGLSF CYNCQRPWIT DPGRPGSSAL TESMHVCYAR SMDKSNTGEE CVAPFFDVRR
MRRRDSILMD PPLKSSLGPL DTVLPRQNSS ISEHANDVQS TDYPELRSCV DHAPLDDKIT
QGDAQTDSNP TRTLSEFMIN KPAPVGGKIK PCPKCNTFIQ KVDDGSCNDM VCAICGFNFC
WLCLRPNSAG HYLVLDGCSI WGKRRWSTRR RLLVLLAVLL LTPFALPLLL AIGIPVGTIS
FVALSCQLVR SRFPTNKHVR WFAVFCTCLG TLIFFPILTG FNLRRFLCTI
//
