ID A0A4S4EBR6_CAMSI Unreviewed; 1200 AA.
AC A0A4S4EBR6;
DT 31-JUL-2019, integrated into UniProtKB/TrEMBL.
DT 31-JUL-2019, sequence version 1.
DT 18-JUN-2025, entry version 19.
DE RecName: Full=DNA-directed RNA polymerase subunit beta {ECO:0000256|RuleBase:RU363031};
DE EC=2.7.7.6 {ECO:0000256|RuleBase:RU363031};
GN ORFNames=TEA_007388 {ECO:0000313|EMBL:THG13649.1};
OS Camellia sinensis var. sinensis.
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; Ericales; Theaceae; Camellia.
OX NCBI_TaxID=542762 {ECO:0000313|EMBL:THG13649.1, ECO:0000313|Proteomes:UP000306102};
RN [1] {ECO:0000313|EMBL:THG13649.1, ECO:0000313|Proteomes:UP000306102}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Shuchazao {ECO:0000313|Proteomes:UP000306102};
RC TISSUE=Leaf {ECO:0000313|EMBL:THG13649.1};
RX PubMed=29678829; DOI=.1073/pnas.1719622115;
RA Wei C., Yang H., Wang S., Zhao J., Liu C., Gao L., Xia E., Lu Y., Tai Y.,
RA She G., Sun J., Cao H., Tong W., Gao Q., Li Y., Deng W., Jiang X., Wang W.,
RA Chen Q., Zhang S., Li H., Wu J., Wang P., Li P., Shi C., Zheng F., Jian J.,
RA Huang B., Shan D., Shi M., Fang C., Yue Y., Li F., Li D., Wei S., Han B.,
RA Jiang C., Yin Y., Xia T., Zhang Z., Bennetzen J.L., Zhao S., Wan X.;
RT "Draft genome sequence of Camellia sinensis var. sinensis provides insights
RT into the evolution of the tea genome and tea quality.";
RL Proc. Natl. Acad. Sci. U.S.A. 115:E4151-E4158(2018).
CC -!- FUNCTION: DNA-dependent RNA polymerase catalyzes the transcription of
CC DNA into RNA using the four ribonucleoside triphosphates as substrates.
CC {ECO:0000256|RuleBase:RU363031}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) +
CC diphosphate; Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC EC=2.7.7.6; Evidence={ECO:0000256|ARBA:ARBA00048552,
CC ECO:0000256|RuleBase:RU363031};
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000256|ARBA:ARBA00004123}.
CC -!- SIMILARITY: Belongs to the RNA polymerase beta chain family.
CC {ECO:0000256|ARBA:ARBA00006835, ECO:0000256|RuleBase:RU000434}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:THG13649.1}.
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DR EMBL; SDRB02005770; THG13649.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4S4EBR6; -.
DR STRING; 542762.A0A4S4EBR6; -.
DR Proteomes; UP000306102; Unassembled WGS sequence.
DR GO; GO:0000428; C:DNA-directed RNA polymerase complex; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; IEA:GOC.
DR GO; GO:0009536; C:plastid; IEA:GOC.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003899; F:DNA-directed RNA polymerase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0032549; F:ribonucleoside binding; IEA:InterPro.
DR GO; GO:0006351; P:DNA-templated transcription; IEA:InterPro.
DR CDD; cd00653; RNA_pol_B_RPB2; 1.
DR FunFam; 2.40.50.150:FF:000002; DNA-directed RNA polymerase subunit beta; 1.
DR FunFam; 3.90.1100.10:FF:000003; DNA-directed RNA polymerase subunit beta; 1.
DR FunFam; 3.90.1100.10:FF:000005; DNA-directed RNA polymerase subunit beta; 1.
DR FunFam; 3.90.1110.10:FF:000005; DNA-directed RNA polymerase subunit beta; 1.
DR FunFam; 3.90.1800.10:FF:000002; DNA-directed RNA polymerase subunit beta; 1.
DR Gene3D; 2.40.50.150; -; 1.
DR Gene3D; 3.90.1100.10; -; 1.
DR Gene3D; 2.40.270.10; DNA-directed RNA polymerase, subunit 2, domain 6; 1.
DR Gene3D; 3.90.1800.10; RNA polymerase alpha subunit dimerisation domain; 1.
DR Gene3D; 3.90.1110.10; RNA polymerase Rpb2, domain 2; 1.
DR InterPro; IPR015712; DNA-dir_RNA_pol_su2.
DR InterPro; IPR007120; DNA-dir_RNAP_su2_dom.
DR InterPro; IPR037033; DNA-dir_RNAP_su2_hyb_sf.
DR InterPro; IPR007121; RNA_pol_bsu_CS.
DR InterPro; IPR007644; RNA_pol_bsu_protrusion.
DR InterPro; IPR007642; RNA_pol_Rpb2_2.
DR InterPro; IPR037034; RNA_pol_Rpb2_2_sf.
DR InterPro; IPR007645; RNA_pol_Rpb2_3.
DR InterPro; IPR007646; RNA_pol_Rpb2_4.
DR InterPro; IPR007647; RNA_pol_Rpb2_5.
DR InterPro; IPR007641; RNA_pol_Rpb2_7.
DR InterPro; IPR014724; RNA_pol_RPB2_OB-fold.
DR NCBIfam; NF007175; PRK09606.1; 1.
DR PANTHER; PTHR20856; DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2; 1.
DR Pfam; PF04563; RNA_pol_Rpb2_1; 1.
DR Pfam; PF04561; RNA_pol_Rpb2_2; 1.
DR Pfam; PF04565; RNA_pol_Rpb2_3; 1.
DR Pfam; PF04566; RNA_pol_Rpb2_4; 1.
DR Pfam; PF04567; RNA_pol_Rpb2_5; 1.
DR Pfam; PF00562; RNA_pol_Rpb2_6; 1.
DR Pfam; PF04560; RNA_pol_Rpb2_7; 1.
DR SUPFAM; SSF64484; beta and beta-prime subunits of DNA dependent RNA-polymerase; 1.
DR PROSITE; PS01166; RNA_POL_BETA; 1.
PE 3: Inferred from homology;
KW DNA-directed RNA polymerase {ECO:0000256|ARBA:ARBA00022478,
KW ECO:0000256|RuleBase:RU363031}; Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695,
KW ECO:0000256|RuleBase:RU363031}; Nucleus {ECO:0000256|ARBA:ARBA00023242};
KW Reference proteome {ECO:0000313|Proteomes:UP000306102};
KW Transcription {ECO:0000256|ARBA:ARBA00023163,
KW ECO:0000256|RuleBase:RU363031};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU363031};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771}.
FT DOMAIN 50..459
FT /note="RNA polymerase beta subunit protrusion"
FT /evidence="ECO:0000259|Pfam:PF04563"
FT DOMAIN 220..411
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04561"
FT DOMAIN 504..550
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04565"
FT DOMAIN 585..646
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04566"
FT DOMAIN 671..722
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04567"
FT DOMAIN 729..1099
FT /note="DNA-directed RNA polymerase subunit 2 hybrid-
FT binding"
FT /evidence="ECO:0000259|Pfam:PF00562"
FT DOMAIN 1101..1192
FT /note="RNA polymerase Rpb2"
FT /evidence="ECO:0000259|Pfam:PF04560"
SQ SEQUENCE 1200 AA; 135985 MW; D5C83CC132352AAE CRC64;
MYGGDEYDPS MNYHGDGVVE AYDEDEEEEE QEITQEDAWA VISAYFEEKG LVRQQLDSFD
EFIQNTMQEI VDESAAIEIR PESQHNPGRH ADFVETIYRI SFGQIYLSKP MMTESDGETA
SLFPKAARLR NLTYSSPLYV DVTKRVVKKG HDCEEVTETQ EFTKVFIGKV PIMLRSSYCC
LYQTSEKDLT ELGECPFDQG GYFIINGSEK VLIAQEKMST NHVYVFKKRQ PNKYAFVAEV
RSMAESQNRP PSGMFVRMLS TTSAKGGSSG QYIRATLPYI RTEVPIVVVF RALGFVADKD
ILEHICYEFS DIQMMDLLRP SLEEAFVIQN QQVALDYIGK RGSTVGVTRD KRIRYAKEIL
QREMLPHVGT GEYCETKKAY YFGYIIHRLL LCALGRRPED DRDHYANKRL DLAGPLLGGL
FRMLFRKLTR DVRSYVQKCV DNGKDVNLQF AIKAKSITSG LKYSLATGNW GQANAAGTRA
GVSQVGSYLI HFILLDVLLF NSFMGSFMST GKLAKPRQLH NSHWGMMCPA ETPEGQACGL
VKNLALMVYI TVGSAAHPIL EFLEEWSTEN FEEISPAVIP QATKIFVNGC WVGIHRNPEL
LVKTLRQLRR QVDVNTEVGV IRDIRLKELR LYTDYGRCSR PLFIVDKQKL LIKKKDILIL
QQRESPEESG WHDLVAKGFI EYVDTEEEET TMISMTINDL VTSRVNPDEA YSETYTHCEI
HPSLILGVCA SIIPFPDHNQ SPRNTYQSAM GKQAMGIYVT NYQLRMDTLA YVLYYPQKPL
VTTRAMEHLH FRQLPAGINA TVAIACYSGY NQEDSLIMNQ SSIDRGFFRS LFFRSYRDEE
KKMGTLIKED FGCPNRDNTM GMRHGSYDKL DDDGLAPPGT RVSGEDVIIG KTTPMVPEET
QGPSSRYTRR DHSTALRHSE SGMVDQVLLT TNADGLRFVK IRMRSVRIPQ IGDKFSSRHG
QKGTVGMTYT QEDMPWSIEG ITPDIIVNPH AIPSRMTVGQ LIECIMGKVA AHVGKEGDAT
PFTDVTLDNI SKALHKCGYQ MRGFETMYNG HTGRQLTSMI FLGPTYYQRL KHMVDDKIHS
RGRGPVQILT RQPAEGRSRD GGLRFGEMER DCMIAHGAAH FLKERLFDQS DAYRVHVCEH
CGLVAIANLK KNSFECRSCK NKTDIVQVYL PYACKLLIQE LMSMAIATRL LTNEVKLSKE
//
