ID   A0A4W5N518_9TELE        Unreviewed;       413 AA.
AC   A0A4W5N518;
DT   18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT   18-SEP-2019, sequence version 1.
DT   10-JUN-2026, entry version 24.
DE   RecName: Full=Cytoplasmic tRNA 2-thiolation protein 2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN   Name=CTU2 {ECO:0000256|HAMAP-Rule:MF_03054};
GN   Synonyms=NCS2 {ECO:0000256|HAMAP-Rule:MF_03054};
OS   Hucho hucho (huchen).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Protacanthopterygii; Salmoniformes;
OC   Salmonidae; Salmoninae; Hucho.
OX   NCBI_TaxID=62062 {ECO:0000313|Ensembl:ENSHHUP00000045797.1, ECO:0000313|Proteomes:UP000314982};
RN   [1] {ECO:0000313|Ensembl:ENSHHUP00000045797.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (AUG-2025) to UniProtKB.
RN   [2] {ECO:0000313|Ensembl:ENSHHUP00000045797.1}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (SEP-2025) to UniProtKB.
CC   -!- FUNCTION: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA
CC       wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). May act by
CC       forming a heterodimer with CTU1/ATPBD3 that ligates sulfur from
CC       thiocarboxylated URM1 onto the uridine of tRNAs at wobble position.
CC       {ECO:0000256|HAMAP-Rule:MF_03054}.
CC   -!- PATHWAY: tRNA modification; 5-methoxycarbonylmethyl-2-thiouridine-tRNA
CC       biosynthesis. {ECO:0000256|HAMAP-Rule:MF_03054}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|HAMAP-Rule:MF_03054}.
CC   -!- SIMILARITY: Belongs to the CTU2/NCS2 family. {ECO:0000256|HAMAP-
CC       Rule:MF_03054}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; A0A4W5N518; -.
DR   Ensembl; ENSHHUT00000047489.1; ENSHHUP00000045797.1; ENSHHUG00000027903.1.
DR   GeneTree; ENSGT00390000008797; -.
DR   UniPathway; UPA00988; -.
DR   Proteomes; UP000314982; Unassembled WGS sequence.
DR   GO; GO:0005829; C:cytosol; IEA:TreeGrafter.
DR   GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0016783; F:sulfurtransferase activity; IEA:TreeGrafter.
DR   GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR   GO; GO:0032447; P:protein urmylation; IEA:UniProtKB-UniRule.
DR   GO; GO:0002143; P:tRNA wobble position uridine thiolation; IEA:TreeGrafter.
DR   Gene3D; 3.40.50.620; HUPs; 1.
DR   HAMAP; MF_03054; CTU2; 1.
DR   InterPro; IPR019407; CTU2.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   PANTHER; PTHR20882; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR   PANTHER; PTHR20882:SF14; CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 2; 1.
DR   Pfam; PF10288; CTU2; 1.
DR   SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE   3: Inferred from homology;
KW   Cytoplasm {ECO:0000256|ARBA:ARBA00022490, ECO:0000256|HAMAP-Rule:MF_03054};
KW   Reference proteome {ECO:0000313|Proteomes:UP000314982};
KW   tRNA processing {ECO:0000256|ARBA:ARBA00022694, ECO:0000256|HAMAP-
KW   Rule:MF_03054}.
SQ   SEQUENCE   413 AA;  45811 MW;  721242983D64858C CRC64;
     MCQVDEEYNG QLEHRGAPRV SKKCVKCKEN TAVLIIRAGD AFCRGCFKDY FIHKFRAMLG
     KNRVIFPGEK VLLAVSGGPA SISMLSQVQE GLSRDAPKKL RFVPGIIYID DGGASGQSME
     ERKRAVAQLE SVFRATNYHY HIVHLEQVSS LPSSVLEAGS SATEKPETGL SYKAAVDHFI
     QTRKNQNQHS DLSVENGQSH LSSLSMQEVQ NEVTSPITPE HTQALQRLFA SVRTLTAKED
     LLHTLRQHLI LHTARTQGYT KVMMGDSCSR LAIKLLSNIS LGRGASLAMD TGFSDPRYGD
     VVIVRPMRDY SSKEIAYYNR MFDIPSVFIP GLDTKAQDKA SIQRLTESFV TKLQTDFPST
     VSTIYRTSEK LHTACPPQNT NTQLSAKCLL CVCALDTKLG DCIFHLNCNT LIT
//