ID A0A4W6DN36_LATCA Unreviewed; 857 AA.
AC A0A4W6DN36;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 18-JUN-2025, entry version 26.
DE RecName: Full=Phospholipase {ECO:0000256|PIRNR:PIRNR009376};
DE EC=3.1.4.4 {ECO:0000256|PIRNR:PIRNR009376};
GN Name=pld1a {ECO:0000313|Ensembl:ENSLCAP00010026192.1};
OS Lates calcarifer (Barramundi) (Holocentrus calcarifer).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Neoteleostei; Acanthomorphata;
OC Carangaria; Carangaria incertae sedis; Centropomidae; Lates.
OX NCBI_TaxID=8187 {ECO:0000313|Ensembl:ENSLCAP00010026192.1, ECO:0000313|Proteomes:UP000314980};
RN [1] {ECO:0000313|Proteomes:UP000314980}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Sai Rama Sridatta P.;
RL Submitted (SEP-2015) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLCAP00010026192.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (MAR-2025) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phosphocholine + H2O = a 1,2-diacyl-
CC sn-glycero-3-phosphate + choline + H(+); Xref=Rhea:RHEA:14445,
CC ChEBI:CHEBI:15354, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57643, ChEBI:CHEBI:58608; EC=3.1.4.4;
CC Evidence={ECO:0000256|PIRNR:PIRNR009376};
CC -!- SUBCELLULAR LOCATION: Endomembrane system
CC {ECO:0000256|ARBA:ARBA00037868}; Lipid-anchor
CC {ECO:0000256|ARBA:ARBA00037868}.
CC -!- SIMILARITY: Belongs to the phospholipase D family.
CC {ECO:0000256|ARBA:ARBA00008664, ECO:0000256|PIRNR:PIRNR009376}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR AlphaFoldDB; A0A4W6DN36; -.
DR Ensembl; ENSLCAT00010026746.1; ENSLCAP00010026192.1; ENSLCAG00010011374.1.
DR GeneTree; ENSGT00940000155015; -.
DR Proteomes; UP000314980; Unassembled WGS sequence.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IEA:TreeGrafter.
DR GO; GO:0035091; F:phosphatidylinositol binding; IEA:InterPro.
DR GO; GO:0004630; F:phospholipase D activity; IEA:UniProtKB-UniRule.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:InterPro.
DR GO; GO:0006654; P:phosphatidic acid biosynthetic process; IEA:InterPro.
DR GO; GO:0009395; P:phospholipid catabolic process; IEA:TreeGrafter.
DR GO; GO:0032534; P:regulation of microvillus assembly; IEA:TreeGrafter.
DR GO; GO:0060627; P:regulation of vesicle-mediated transport; IEA:TreeGrafter.
DR CDD; cd01254; PH_PLD; 1.
DR FunFam; 2.30.29.30:FF:000114; Phospholipase; 1.
DR FunFam; 3.30.870.10:FF:000005; Phospholipase; 1.
DR FunFam; 3.30.870.10:FF:000018; Phospholipase; 1.
DR Gene3D; 3.30.870.10; Endonuclease Chain A; 3.
DR Gene3D; 3.30.1520.10; Phox-like domain; 1.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR001849; PH_domain.
DR InterPro; IPR025202; PLD-like_dom.
DR InterPro; IPR001736; PLipase_D/transphosphatidylase.
DR InterPro; IPR016555; PLipase_D_euk.
DR InterPro; IPR015679; PLipase_D_fam.
DR InterPro; IPR036871; PX_dom_sf.
DR PANTHER; PTHR18896; PHOSPHOLIPASE D; 1.
DR PANTHER; PTHR18896:SF57; PHOSPHOLIPASE D1; 1.
DR Pfam; PF00169; PH; 1.
DR Pfam; PF00614; PLDc; 1.
DR Pfam; PF13091; PLDc_2; 1.
DR PIRSF; PIRSF009376; Phospholipase_D_euk; 2.
DR SMART; SM00233; PH; 1.
DR SMART; SM00155; PLDc; 2.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR SUPFAM; SSF56024; Phospholipase D/nuclease; 2.
DR SUPFAM; SSF64268; PX domain; 1.
DR PROSITE; PS50003; PH_DOMAIN; 1.
DR PROSITE; PS50035; PLD; 2.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR009376};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963,
KW ECO:0000256|PIRNR:PIRNR009376};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|ARBA:ARBA00023136};
KW Phosphoprotein {ECO:0000256|ARBA:ARBA00022553};
KW Reference proteome {ECO:0000313|Proteomes:UP000314980};
KW Repeat {ECO:0000256|ARBA:ARBA00022737}.
FT DOMAIN 141..250
FT /note="PH"
FT /evidence="ECO:0000259|PROSITE:PS50003"
FT DOMAIN 381..408
FT /note="PLD phosphodiesterase"
FT /evidence="ECO:0000259|PROSITE:PS50035"
FT DOMAIN 674..701
FT /note="PLD phosphodiesterase"
FT /evidence="ECO:0000259|PROSITE:PS50035"
SQ SEQUENCE 857 AA; 99499 MW; CE4141B931089EF3 CRC64;
CRIPFSAVYA TVGFKEASAR VYLPTVPITA RILEVERFTT SLPAVFKIEL KHGEFTWVVK
RKEKHFMELH RELRTYKTFM RIPLPSRRLA PTHLDLHSHM DMHMYKHQQT QDLILLLSLP
VYSQMEFIDV SQLSFIHDLG PKGLEGMIYK RSGGHRIPGM NCCGHSQACY RWSKRWLVVK
DSCLLYMKPD SGAISFVLLV DKEFSIKMDS KDTETKHGVR IDSLSRTLVF KCSSYRHARW
WGQSIESFVR NHGKAFLRDH RFGSFAQEQE NIPAKWYVNG KTYMEDVADA LEEAKEEIFI
TDWWLSPEIF LKRPVVEGNR WRLDCILKRK AQQGVHIFVM LYKEVELALG INSGYSKRTL
MHLHPNIKVM RHPDHVSSSV YLWAHHEKIV VIDQSVAFVG GIDLAYGRWD DREHRLTDVG
SVTRSVALEQ QGNTRFWHGK DYCNFVYKDW IQLEKPFDDF IDRYTTPRMP WHDIASVVHG
RAARDVARHF IQRWNFTKAP KYRSLSYPFL LPKSHSSADD LRYQVPECVD AKVQVLRSSA
DWSAGIKYHE ESIHNAYIQV IAKSKHYIYI ENQFFITHKL YSLWFCFLQQ HCKSGKKYRV
YVVTPLLPGF EGDITTGGGN AIQAVMHFNY RTMIRGEYSI ISQLKREMDD QWMNYISFAG
LRTHAELEGR LVTELIYVHS KMLIADDNTV IIGSANINDR SMLGKRDSEV AVIVEDSQKV
ASVMDGQEYE AGPYALQLRL ECFRTILGGH TDTSIDLSDP ISDRFYKEVW MTTAGRNATI
YEKVFRCLPS SLVRNMSELE QYQSKPGLAQ SDLTRAQEEL RKIRGFLVQF PLDFLSEHNL
MPSVGTKESM VPTEIWT
//
