ID A0A4Y2CFM8_ARAVE Unreviewed; 969 AA.
AC A0A4Y2CFM8;
DT 18-SEP-2019, integrated into UniProtKB/TrEMBL.
DT 18-SEP-2019, sequence version 1.
DT 28-JAN-2026, entry version 21.
DE RecName: Full=Glucosidase II subunit alpha {ECO:0000256|ARBA:ARBA00042895};
GN Name=GANAB_1 {ECO:0000313|EMBL:GBM03212.1};
GN ORFNames=AVEN_142514_2 {ECO:0000313|EMBL:GBM03212.1};
OS Araneus ventricosus (Orbweaver spider) (Epeira ventricosa).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC Araneomorphae; Entelegynae; Araneoidea; Araneidae; Araneus.
OX NCBI_TaxID=182803 {ECO:0000313|EMBL:GBM03212.1, ECO:0000313|Proteomes:UP000499080};
RN [1] {ECO:0000313|EMBL:GBM03212.1, ECO:0000313|Proteomes:UP000499080}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=31182776;
RA Kono N., Nakamura H., Ohtoshi R., Moran D.A.P., Shinohara A., Yoshida Y.,
RA Fujiwara M., Mori M., Tomita M., Arakawa K.;
RT "Orb-weaving spider Araneus ventricosus genome elucidates the spidroin gene
RT catalogue.";
RL Sci. Rep. 9:8380-8380(2019).
CC -!- PATHWAY: Glycan metabolism; N-glycan metabolism.
CC {ECO:0000256|ARBA:ARBA00004833}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum
CC {ECO:0000256|ARBA:ARBA00004240}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 31 family.
CC {ECO:0000256|ARBA:ARBA00007806, ECO:0000256|RuleBase:RU361185}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GBM03212.1}.
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DR EMBL; BGPR01000189; GBM03212.1; -; Genomic_DNA.
DR AlphaFoldDB; A0A4Y2CFM8; -.
DR OrthoDB; 3237269at2759; -.
DR Proteomes; UP000499080; Unassembled WGS sequence.
DR GO; GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
DR GO; GO:0090599; F:alpha-glucosidase activity; IEA:TreeGrafter.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR GO; GO:0006491; P:N-glycan processing; IEA:TreeGrafter.
DR CDD; cd06603; GH31_GANC_GANAB_alpha; 1.
DR CDD; cd14752; GH31_N; 1.
DR FunFam; 3.20.20.80:FF:000039; Glucosidase, alpha neutral C; 1.
DR FunFam; 2.60.40.1180:FF:000023; neutral alpha-glucosidase AB isoform X2; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.1760; glycosyl hydrolase (family 31); 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 2.
DR InterPro; IPR033403; DUF5110.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR017853; GH.
DR InterPro; IPR048395; Glyco_hydro_31_C.
DR InterPro; IPR025887; Glyco_hydro_31_N_dom.
DR InterPro; IPR000322; Glyco_hydro_31_TIM.
DR InterPro; IPR013780; Glyco_hydro_b.
DR PANTHER; PTHR22762; ALPHA-GLUCOSIDASE; 1.
DR PANTHER; PTHR22762:SF54; BCDNA.GH04962; 1.
DR Pfam; PF17137; DUF5110; 1.
DR Pfam; PF13802; Gal_mutarotas_2; 1.
DR Pfam; PF01055; Glyco_hydro_31_2nd; 1.
DR Pfam; PF21365; Glyco_hydro_31_3rd; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF51011; Glycosyl hydrolase domain; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361185};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361185};
KW Reference proteome {ECO:0000313|Proteomes:UP000499080};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..969
FT /note="Glucosidase II subunit alpha"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5021443323"
FT DOMAIN 184..350
FT /note="Glycoside hydrolase family 31 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF13802"
FT DOMAIN 411..739
FT /note="Glycoside hydrolase family 31 TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01055"
FT DOMAIN 747..835
FT /note="Glycosyl hydrolase family 31 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF21365"
FT DOMAIN 853..909
FT /note="DUF5110"
FT /evidence="ECO:0000259|Pfam:PF17137"
FT REGION 215..244
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 215..225
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 969 AA; 111684 MW; 1591C04BC2840F17 CRC64;
MATSKRHFHI FYILILFVIQ PFTECVDRSL FKTCEQSGFC KRHRSTERRQ SPYYLEMSSF
KIYPTRIEGV IINSQNGVML KLDLVALKNN MLNFKISELN PIRPRYEARE SLVSDPEESS
LEVLSQDSEK VVVGFGPNKA VLNGSPFRID VYSGDLLLLS ANARVLKSCL KIQKKLLIWS
KQSCFEWKPF RIDVYSGDLL LLSANARGLM KFEHYRPKTK GKPEGEGEDQ NEMQQQPPLS
QDEEQFKETL WEETFKGHTD TKPNGPASVG MDFSFVGFEH VYGIPEHADV FPLKTTKGAS
DPYRLFNLDV FEYELNNPMA LYASIPFMVA HSETNSLGLF WLNAAETWVD IEAPSNQGVV
SSIVDFVKGS SQIPQRDTHW FSESGLINVF ILLGPTPKDV MFQYSKLTGS TPLPPLFSLG
YHQSRWNYRD QDDVRQVDAA FDENDIPYDV LWLDLDHTHD RKYFTWEPFK FSDPIGMINN
LTAKGRKMVT LVDPHIKRDS GYYIHKEATD KGFYVKNRDN NDYDGWCWPG SSGYLDFLSP
EIRDWWASKF ALDQYQGSTL ALYTWNDMNE PSVFNGPEVT MERDCKHVGG WEHRDVHNIY
GMLMVMSTYK GHLMRSNGAQ RPFILTRSAF AGSQRFGAIW TGDNIAEWEH LQMSVPMVLS
LSLCGMSHSG ADIGGFFRNP SPELNMRWYQ AGAFQPFFRA HSHHETRRRE PWLFDENYKN
LIREAVRIRY SFLPYWYTLF YENELTGVPP MRPIWMEFPT ERATFDLSDE YMIGDSLLVR
PVMQADVISI NMYFPGTGEV WYDFITHQQH EGGQMLTIPV TLDKIPVFQR GGTIIPKKGR
VRRSSSLTYN DPYTLEIALD KTGSRANGTL YIDDFNSFEY RNGNYLLLKF TYEKNTITSK
IIGGPGKFKT NAWLEKIIVI GVQKPPKSFT LSSKSIKNSE LLFTYDEAKQ KLVIRKPGVN
MGEEWKITF
//
